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1. 发明申请

US20060121045A1 Antibody fragments for protection from pathogen infection and methods of use thereof 审中-公开
标题翻译：用于防止病原体感染的抗体片段及其使用方法
公开(公告)号：US20060121045A1
公开(公告)日：2006-06-08
申请号：US11270894
申请日：2005-11-08
申请人： Brent Iverson , George Georgiou , Robert Mabry
发明人： Brent Iverson , George Georgiou , Robert Mabry
IPC分类号： A61K39/40 , A61K39/07
CPC分类号： C07K16/1278 , A61K2039/505 , C07K2317/54 , C07K2317/55 , C07K2317/56 , C07K2317/622 , C07K2317/76
摘要： Methods and compositions are provided for an antibody fragment or functional fragment thereof, which fragments do not contain an Fc region. The invention also describes in vitro and in vivo treatment of a subject exposed to a pathogen, pathogen infection, or pathogen protein product by administering to the subject a therapeutically effective amount of the described antibody fragments and functional fragments thereof.
摘要翻译：提供了抗体片段或其功能片段的方法和组合物，其片段不含有Fc区。本发明还描述了暴露于病原体，病原体感染或病原体蛋白质产物的受试者的体外和体内治疗，其通过向受试者施用治疗有效量的所述抗体片段及其功能性片段。

2. 发明申请

US20070065913A1 ISOLATION OF BINDING PROTEINS WITH HIGH AFFINITY TO LIGANDS 有权
标题翻译：分离具有高亲和力的结合蛋白
公开(公告)号：US20070065913A1
公开(公告)日：2007-03-22
申请号：US11461757
申请日：2006-08-01
申请人： Gang Chen , Andrew Hayhurst , Jeffrey Thomas , Brent Iverson , George Georgiou
发明人： Gang Chen , Andrew Hayhurst , Jeffrey Thomas , Brent Iverson , George Georgiou
IPC分类号： C12P21/06 , C07H21/04 , C12N9/00 , C12N1/21 , C07K14/195 , C12N15/74
CPC分类号： C12N15/1034
摘要： The invention overcomes the deficiencies of the prior art by providing a rapid approach for isolating binding proteins capable of binding small molecules and peptides via “display-less” library screening. In the technique, libraries of candidate binding proteins, such as antibody sequences, are expressed in soluble form in the periplasmic space of gram negative bacteria, such as Escherichia coli, and are mixed with a labeled ligand. In clones expressing recombinant polypeptides with affinity for the ligand, the concentration of the labeled ligand bound to the binding protein is increased and allows the cells to be isolated from the rest of the library. Where fluorescent labeling of the target ligand is used, cells may be isolated by fluorescence activated cell sorting (FACS). The approach is more rapid than prior art methods and avoids problems associated with the surface-expression of ligand fusion proteins employed with phage display.
摘要翻译：本发明通过提供通过“无显示”文库筛选分离能够结合小分子和肽的结合蛋白的快速方法来克服现有技术的缺陷。在该技术中，候选结合蛋白（例如抗体序列）的文库以可溶形式表达在革兰氏阴性细菌如大肠杆菌的周质空间中，并与标记的配体混合。在表达对配体具有亲和性的重组多肽的克隆中，与结合蛋白结合的标记配体的浓度增加，并允许细胞与文库的其余部分分离。当使用靶配体的荧光标记时，可以通过荧光激活细胞分选（FACS）分离细胞。该方法比现有技术方法更快速，并避免与噬菌体展示所用配体融合蛋白的表面表达相关的问题。

3. 发明申请

US20050267294A1 Antibodies with increased affinities for anthrax antigens 有权
公开(公告)号：US20050267294A1
公开(公告)日：2005-12-01
申请号：US11179244
申请日：2005-07-12
申请人： Barrett Harvey , George Georgiou , Brent Iverson
发明人： Barrett Harvey , George Georgiou , Brent Iverson
IPC分类号： A61K39/00 , A61K39/07 , A61K39/395 , A61K39/40 , C07H21/04 , C07K7/00 , C07K16/00 , C07K16/12 , C12N20060101 , C12N1/21 , C12N15/10 , C12N15/13 , C12N15/70 , C12Q1/02 , C12Q1/04 , G01N33/554 , G01N33/569 , G01N33/68
CPC分类号： C07K16/005 , C07K2317/622 , C07K2317/92 , C12N15/1034 , C12Q1/025 , G01N33/6845
摘要： The invention overcomes the deficiencies of the prior art by providing antibody compositions having improved affinities for Bacillus anthracis antigens. The compositions have important thereapeutic and diagnostic applications, including treatment or detection of infection by Bacillus anthracis.

4. 发明申请

US20050260736A1 Selection of bacterial inner-membrane anchor polypeptides 有权
标题翻译：细菌内膜锚定多肽的选择
公开(公告)号：US20050260736A1
公开(公告)日：2005-11-24
申请号：US11084717
申请日：2005-03-18
申请人： George Georgiou , Ki Jun Jeong , Barrett Harvey , Brent Iverson
发明人： George Georgiou , Ki Jun Jeong , Barrett Harvey , Brent Iverson
IPC分类号： C12N1/21 , C12N15/10 , C12N15/74 , C40B40/02
CPC分类号： C40B40/02 , C07K2319/04 , C12N15/1037 , C12N15/1086
摘要： The invention overcomes the deficiencies of the prior art by providing a rapid approach for isolating polypeptides capable of anchoring heterologous polypeptides to a bacterial inner membrane. In the technique, libraries of candidate anchor polypeptides are expressed as fusions with a heterologous polypeptide that is capable of being detected when bound to the inner membrane. In bacteria expressing a functional anchor sequence, the heterologous polypeptide becomes bound to outer face of the inner membrane. Bacteria with the functional anchor sequence can be identified by removing the outer membrane to remove non-anchored heterologous polypeptide followed by detection of anchored heterologous polypeptide. Such bacteria may be detected in numerous ways, including use of direct fluorescence or secondary antibodies that are fluorescently labeled, allowing use of efficient techniques such as fluorescence activated cell sorting (FACS).
摘要翻译：本发明克服了现有技术的缺陷，提供了一种用于分离能够将异源多肽固定在细菌内膜上的多肽的快速方法。在该技术中，候选锚多肽的文库表达为与异源多肽的融合，当与内膜结合时能够被检测。在表达功能性锚定序列的细菌中，异源多肽与内膜的外表面结合。具有功能性锚定序列的细菌可以通过除去外膜以除去非锚定的异源多肽，然后检测锚定的异源多肽来鉴定。可以以许多方式检测这些细菌，包括使用荧光标记的直接荧光或二次抗体，允许使用有效技术，例如荧光激活细胞分选（FACS）。

5. 发明申请

US20050106647A1 ANTIBODIES WITH INCREASED AFFINITIES FOR ANTHRAX ANTIGENS 有权
标题翻译：抗体对ANTHRAX抗原具有增加的作用
公开(公告)号：US20050106647A1
公开(公告)日：2005-05-19
申请号：US10620049
申请日：2003-07-15
申请人： Barrett Harvey , George Georgiou , Brent Iverson
发明人： Barrett Harvey , George Georgiou , Brent Iverson
IPC分类号： A61K39/00 , A61K39/07 , A61K39/395 , A61K39/40 , C07H21/04 , C07K7/00 , C07K16/00 , C07K16/12 , C12N20060101 , C12N1/21 , C12N15/10 , C12N15/13 , C12N15/70 , C12Q1/02 , C12Q1/04 , G01N33/554 , G01N33/569 , G01N33/68
CPC分类号： C07K16/005 , C07K2317/622 , C07K2317/92 , C12N15/1034 , C12Q1/025 , G01N33/6845
摘要： The invention overcomes the deficiencies of the prior art by providing antibody compositions having improved affinities for Bacillus anthracis antigens. The compositions have important thereapeutic and diagnostic applications, including treatment or detection of infection by Bacillus anthracis.
摘要翻译：本发明通过提供具有改善的炭疽芽孢杆菌抗原亲和力的抗体组合来克服现有技术的缺陷。该组合物具有重要的治疗和诊断应用，包括炭疽杆菌感染的治疗或检测。

6. 发明申请

US20060029947A1 Combinatorial protein library screening by periplasmic expression 审中-公开
标题翻译：组织蛋白质文库筛选周质表达
公开(公告)号：US20060029947A1
公开(公告)日：2006-02-09
申请号：US11084055
申请日：2005-03-18
申请人： George Georgiou , Ki Jeong , Brent Iverson
发明人： George Georgiou , Ki Jeong , Brent Iverson
IPC分类号： C40B40/02 , C12Q1/68 , G01N33/53 , C12P21/06 , C12N15/74
CPC分类号： C12N15/1086 , C07K2319/02 , C07K2319/03 , C07K2319/034 , C07K2319/21 , C12N15/1037 , C40B40/02 , G01N33/5082 , G01N33/56911 , G01N33/6803
摘要： The invention overcomes the deficiencies of the prior art by providing a rapid approach for isolating binding proteins capable of binding small molecules and peptides. In the technique, libraries of candidate binding proteins, such as antibody sequences, may be expressed in the periplasm of gram negative bacteria with at least one target ligand. In clones expressing recombinant polypeptides with affinity for the ligand, the ligand becomes bound and retained by the cell even after removal of the outer membrane, allowing the cell to be isolated from cells not expressing a binding polypeptide with affinity for the target ligand. The target ligand may be detected in numerous ways, including use of direct fluorescence or secondary antibodies that are fluorescently labeled, allowing use of efficient screening techniques such as fluorescence activated cell sorting (FACS). The approach is more rapid and robust than prior art methods and avoids problems associated with the outer surface-expression of ligand fusion proteins employed with phage display.
摘要翻译：本发明通过提供用于分离能够结合小分子和肽的结合蛋白的快速方法来克服现有技术的缺陷。在该技术中，候选结合蛋白（例如抗体序列）的文库可以用至少一种靶配体在革兰氏阴性细菌的周质中表达。在表达具有对配体具有亲和力的重组多肽的克隆中，甚至在去除外膜之后，配体也被细胞结合并保留，使细胞从不表达具有对靶配体的亲和力的结合多肽的细胞中分离。可以以多种方式检测靶配体，包括使用荧光标记的直接荧光或二次抗体，允许使用有效的筛选技术，例如荧光激活细胞分选（FACS）。该方法比现有技术方法更快速和鲁棒，并且避免与用噬菌体展示使用的配体融合蛋白的外表面表达相关的问题。

7. 发明申请

US20070258954A1 Directed evolution of enzymes and antibodies 审中-公开
标题翻译：酶和抗体的定向进化
公开(公告)号：US20070258954A1
公开(公告)日：2007-11-08
申请号：US11317680
申请日：2005-12-22
申请人： Brent Iverson , George Georgiou , Gang Chen , Mark Olsen , Patrick Daugherty
发明人： Brent Iverson , George Georgiou , Gang Chen , Mark Olsen , Patrick Daugherty
IPC分类号： G01N33/554 , C07K16/00 , C12N1/00
CPC分类号： C07K14/245 , C07K16/00 , C07K16/16 , C07K16/44 , C07K2317/565 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/035 , C12N9/16 , C12N9/86 , C12N15/1037 , C12N15/625 , C40B40/02
摘要： The invention relates to methods of selecting proteins, out of large libraries, having desirable characteristics. Exemplified are methods of expressing enzymes and antibodies on the surface of host cells and selecting for desired activities. These methods have the advantage of speed and ease of operation when compared with current methods. They also provide, without additional cloning, a source of significant quantities of the protein of interest.
摘要翻译：本发明涉及从具有所需特征的大型文库中选择蛋白质的方法。示例是在宿主细胞表面上表达酶和抗体并选择所需活性的方法。与当前的方法相比，这些方法具有速度快和易操作的优点。他们还提供了大量的目标蛋白质来源，无需额外的克隆。

8. 发明申请

US20070099267A1 COMBINATORIAL PROTEIN LIBRARY SCREENING BY PERIPLASMIC EXPRESSION 审中-公开
标题翻译：通过周边表达组合蛋白图书馆筛选
公开(公告)号：US20070099267A1
公开(公告)日：2007-05-03
申请号：US11466352
申请日：2006-08-22
申请人： Barrett Harvey , George Georgiou , Brent Iverson
发明人： Barrett Harvey , George Georgiou , Brent Iverson
IPC分类号： C12P21/06 , C07H21/04 , C12N15/74 , C07K14/245
CPC分类号： C12N15/1034 , C07K2319/034 , C12N15/1037 , C12N15/1086 , C40B40/02
摘要： The invention overcomes the deficiencies of the prior art by providing a rapid approach for isolating binding proteins capable of binding small molecules and peptides. In the technique, libraries of candidate binding proteins, such as antibody sequences, are expressed in the periplasm of gram negative bacteria and mixed with a labeled ligand. In clones expressing recombinant polypeptides with affinity for the ligand, the concentration of the labeled ligand bound to the binding protein is increased and allows the cells to be isolated from the rest of the library. Where fluorescent labeling of the target ligand is used, cells may be isolated by fluorescence activated cell sorting (FACS). The approach is more rapid than prior art methods and avoids problems associated with the outer surface-expression of ligand fusion proteins employed with phage display.
摘要翻译：本发明通过提供用于分离能够结合小分子和肽的结合蛋白的快速方法来克服现有技术的缺陷。在该技术中，候选结合蛋白（例如抗体序列）的文库在革兰氏阴性细菌的周质中表达并与标记的配体混合。在表达对配体具有亲和性的重组多肽的克隆中，与结合蛋白结合的标记配体的浓度增加，并允许细胞与文库的其余部分分离。当使用靶配体的荧光标记时，可以通过荧光激活细胞分选（FACS）分离细胞。该方法比现有技术方法更快速，并且避免了与噬菌体展示一起使用的配体融合蛋白的外表面表达相关的问题。

9. 发明申请

US20060177198A1 Media player configured to receive playback filters from alternative storage mediums 失效
标题翻译：媒体播放器配置为从替代存储介质接收播放过滤器
公开(公告)号：US20060177198A1
公开(公告)日：2006-08-10
申请号：US11327103
申请日：2006-01-05
申请人： Matthew Jarman , Brent Iverson
发明人： Matthew Jarman , Brent Iverson
IPC分类号： H04N5/91
CPC分类号： H04N21/45452 , H04L65/4084 , H04L2012/2849 , H04N5/765 , H04N5/781 , H04N5/85 , H04N5/907 , H04N9/8042 , H04N9/87 , H04N21/435
摘要： A media player configured with a first removable memory reader, such as a DVD drive, and a second removable memory reader, such as a flash memory reader, adapted to communicate with a removable memory containing filter data. The media player is configured to allow filtered playback of a multimedia presentation, such as a movie. Filtered playback causes certain portions of the multimedia presentation to be skipped, muted, blurred, cropped, or otherwise modified to eliminate or reduce potentially objectionable scenes, language, or other content. The second memory reader provides a convenient medium for the loading of filter information, whether data files, executable program code, or the like, to local memory of the media player to employ during filtered playback. Alternatively, the filters may be accessed from the removable storage media during playback rather than loading to local memory.
摘要翻译：配置有诸如DVD驱动器的第一可移动存储器读取器和诸如闪存读取器的第二可移动存储器读取器的媒体播放器，其适于与包含滤波器数据的可移动存储器通信。媒体播放器被配置为允许多媒体呈现（例如电影）的过滤回放。被过滤的播放导致多媒体呈现的某些部分被跳过，静音，模糊，裁剪或以其它方式进行修改，以消除或减少可能令人反感的场景，语言或其他内容。第二存储器读取器提供用于将过滤器信息（无论是数据文件，可执行程序代码等）加载到媒体播放器的本地存储器以在滤波回放期间采用的方便的介质。或者，可以在播放期间从可移动存储介质访问过滤器，而不是加载到本地存储器。

10. 发明申请

US20090210897A9 Method and user interface for downloading audio and video content filters to a media player 有权
标题翻译：用于将音频和视频内容过滤器下载到媒体播放器的方法和用户界面
公开(公告)号：US20090210897A9
公开(公告)日：2009-08-20
申请号：US11256419
申请日：2005-10-20
申请人： Matthew Jarman , Brent Iverson
发明人： Matthew Jarman , Brent Iverson
IPC分类号： H04N5/445 , H04N7/025 , H04N7/18 , H04N7/10
CPC分类号： H04N21/45455 , G06Q30/0601 , G11B27/031 , G11B27/105 , G11B27/32 , H04L65/60 , H04L67/1095 , H04L67/42 , H04N5/765 , H04N5/85 , H04N7/17318 , H04N21/44029 , H04N21/4508 , H04N21/4532 , H04N21/454 , H04N21/4542 , H04N21/472 , H04N21/47815 , H04N21/84 , H04N21/8456 , H04N21/8541
摘要： A client server arrangement for downloading media content filters from a server device to a client device. The media content filters define portions of a separate audio visual presentation containing potentially objectionable subject matter. Depending on user selections, identified portions of the audio/visual presentation may be skipped and/or muted during play. In one particular implementation, the client device, e.g., a DVD player, is configured to initiate a connection with a server device. Upon successful connection, the server device transmits one or more media content filters to the client device. The client device may be configured to determine whether a particular media content filter is available, to facilitate deletion of some existing media content filters in order to secure adequate memory space, and to ensure that the media player has an active account, before initiating a connection with the server device. The server device may be configured to determine whether the media player is associated with an active user account, whether a requested filter is available, and whether adequate memory space is available at the media player, before transmitting media content filters to the client device.
摘要翻译：一种用于从服务器设备向客户端设备下载媒体内容过滤器的客户机服务器装置。媒体内容过滤器定义包含潜在令人反感的主题的单独的视听呈现的部分。根据用户选择，可以在播放期间跳过和/或静音音频/视频呈现的识别部分。在一个特定实现中，客户端设备（例如，DVD播放器）被配置为发起与服务器设备的连接。成功连接后，服务器设备向客户端设备发送一个或多个媒体内容过滤器。客户端设备可以被配置为确定特定的媒体内容过滤器是否可用，以便于删除一些现有的媒体内容过滤器以便确保足够的存储器空间，并且在开始连接之前确保媒体播放器具有活动帐户与服务器设备。服务器设备可以被配置为在向客户端设备传送媒体内容过滤器之前，确定媒体播放器是否与活动用户帐户相关联，无论请求的过滤器是否可用，以及媒体播放器是否有足够的存储空间可用。

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