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1. 发明申请

US20110028352A1 HYBRIDIZATION DEVICE, METHODS, AND SYSTEM USING MIXING BEADS 有权
标题翻译：混合装置，方法和使用混合珠的系统
公开(公告)号：US20110028352A1
公开(公告)日：2011-02-03
申请号：US12847570
申请日：2010-07-30
申请人： Bell Laurent , Martin J. Goldberg , Robert J. Lipshutz , Kaliyur Narasimhan
发明人： Bell Laurent , Martin J. Goldberg , Robert J. Lipshutz , Kaliyur Narasimhan
IPC分类号： C40B50/18 , C40B60/14
CPC分类号： C40B60/12 , B01J2219/00479 , B01J2219/00608 , B01J2219/00659 , B01J2219/00722 , C12Q1/6834
摘要： A method, device and system for hybridizing a target oligonucleotide to at least one array comprising a plurality of mixing beads are provided. A target solution is mixed by agitating the mixing beads while the target oligonucleotides are hybridizing to the complementary probes on the array. In another embodiment, a permeable barrier contains the mixing beads, thereby preventing them from contacting the array surface.
摘要翻译：提供了将目标寡核苷酸与包括多个混合珠的至少一个阵列杂交的方法，装置和系统。通过搅拌混合珠混合目标溶液，同时靶寡核苷酸与阵列上的互补探针杂交。在另一个实施方案中，可渗透屏障包含混合珠，从而防止它们与阵列表面接触。

2. 发明授权

US08445201B2 Hybridization device, methods, and system using mixing beads 有权
标题翻译：杂交装置，方法和使用混合珠的系统
公开(公告)号：US08445201B2
公开(公告)日：2013-05-21
申请号：US12847570
申请日：2010-07-30
申请人： Bellon Laurent , Martin J. Goldberg , Robert J. Lipshutz , Kaliyur Narasimhan
发明人： Bellon Laurent , Martin J. Goldberg , Robert J. Lipshutz , Kaliyur Narasimhan
IPC分类号： C12Q1/68 , C12P19/34 , C12M1/34 , C07H21/02 , C07H21/04
CPC分类号： C40B60/12 , B01J2219/00479 , B01J2219/00608 , B01J2219/00659 , B01J2219/00722 , C12Q1/6834
摘要： A method, device and system for hybridizing a target oligonucleotide to at least one array comprising a plurality of mixing beads are provided. A target solution is mixed by agitating the mixing beads while the target oligonucleotides are hybridizing to the complementary probes on the array. In another embodiment, a permeable barrier contains the mixing beads, thereby preventing them from contacting the array surface.
摘要翻译：提供了将目标寡核苷酸与包括多个混合珠的至少一个阵列杂交的方法，装置和系统。通过搅拌混合珠混合目标溶液，同时靶寡核苷酸与阵列上的互补探针杂交。在另一个实施方案中，可渗透屏障包含混合珠，从而防止它们与阵列表面接触。

3. 发明申请

US20120329677A9 ARRAYS OF NUCLEIC ACID PROBES FOR DETECTING CYSTIC FIBROSIS 审中-公开
标题翻译：用于检测CYSTIC FIBROSIS的核酸探针阵列
公开(公告)号：US20120329677A9
公开(公告)日：2012-12-27
申请号：US11506176
申请日：2006-08-18
申请人： Maureen T. Cronin , Charles Garrett Miyada , Earl A. Hubbell , Mark Chee , Stephen P.A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris , Edward L. Sheldon
发明人： Maureen T. Cronin , Charles Garrett Miyada , Earl A. Hubbell , Mark Chee , Stephen P.A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris , Edward L. Sheldon
IPC分类号： C40B40/06 , C40B50/14
CPC分类号： C12Q1/6874 , C12Q1/6886
摘要： The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the CFTR gene.
摘要翻译：本发明提供了固定化探针的阵列，以及采用阵列的方法，用于检测CFTR基因中的突变。

4. 发明申请

US20120258868A1 Capturing sequences adjacent to type IIs restriction sites for genomic library mapping 审中-公开
标题翻译：捕获与II型限制性位点相邻的序列用于基因组文库作图
公开(公告)号：US20120258868A1
公开(公告)日：2012-10-11
申请号：US13159626
申请日：2011-06-14
申请人： Ronald J. Sapolsky , Robert J. Lipshutz , Thomas R. Gingeras
发明人： Ronald J. Sapolsky , Robert J. Lipshutz , Thomas R. Gingeras
IPC分类号： C40B20/02 , C12Q1/68
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译：本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。本文描述的方法导致捕获和确定位于II型限制性位点附近的特异性寡核苷酸序列。所得序列可用作用于遗传图谱，筛选和操纵的有效标记。

5. 发明申请

US20120172246A1 Detection of Nucleic Acids 审中-公开
标题翻译：核酸检测
公开(公告)号：US20120172246A1
公开(公告)日：2012-07-05
申请号：US13342392
申请日：2012-01-03
申请人： Quan N. Nguyen , Robert J. Lipshutz , Gary K. McMaster , Yunqing Ma , Yen-Chieh Wu , Audrey Lin , Chunfai Lai , Cung-Tuong Nguyen
发明人： Quan N. Nguyen , Robert J. Lipshutz , Gary K. McMaster , Yunqing Ma , Yen-Chieh Wu , Audrey Lin , Chunfai Lai , Cung-Tuong Nguyen
IPC分类号： C40B30/04 , C12Q1/68
CPC分类号： C12Q1/682 , C12Q2525/113 , C12Q2525/161 , C12Q2537/125 , C12Q2537/143
摘要： Methods of detecting various types of nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Detection assays may be conducted at least in vitro, in cellulo, and in situ. Nucleic acids which are optionally captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label probe system with the nucleic acids. Various label probe system embodiments are provided. Compositions, kits, and systems related to the methods are also described.
摘要翻译：提供检测各种类型核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。检测试验可以至少在体外，纤维素和原位进行。可以例如通过导致标记探针系统与核酸的特异性缔合的协同杂交事件来检测任选地捕获在固体支持物上的核酸。提供了各种标签探针系统实施例。还描述了与方法相关的组合物，试剂盒和系统。

6. 发明申请

US20100261616A1 Capturing Sequences Adjacent to type-IIs restriction sites for Genomic Library Mapping 失效
标题翻译：捕获序列与基因组图谱绘制的II型限制性位点相邻
公开(公告)号：US20100261616A1
公开(公告)日：2010-10-14
申请号：US12272680
申请日：2008-11-17
申请人： Ronald J. Sapolsky , Robert J. Lipshutz , Thomas R. Gingeras
发明人： Ronald J. Sapolsky , Robert J. Lipshutz , Thomas R. Gingeras
IPC分类号： C12Q1/68 , C40B30/04 , C40B50/00
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译：本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。所得序列可用作用于遗传图谱，筛选和操纵的有效标记。

7. 发明授权

US07399584B2 Method of comparing a target nucleic acid and a reference nucleic acid 失效
标题翻译：比较目标核酸和参照核酸的方法
公开(公告)号：US07399584B2
公开(公告)日：2008-07-15
申请号：US09510378
申请日：2000-02-22
申请人： Maureen T. Cronin , Charles Garrett Miyada , Earl A. Hubbell , Mark Chee , Stephen P. A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris , Edward L. Sheldon
发明人： Maureen T. Cronin , Charles Garrett Miyada , Earl A. Hubbell , Mark Chee , Stephen P. A. Fodor , Xiaohua C. Huang , Robert J. Lipshutz , Peter E. Lobban , MacDonald S. Morris , Edward L. Sheldon
IPC分类号： C12Q1/68 , C12P19/34 , C12M1/34 , C07H21/00
CPC分类号： C07H21/00 , B01J19/0046 , B01J2219/00432 , B01J2219/00529 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C12Q1/6827 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , C12Q1/6883 , C12Q1/6886 , C12Q2600/106 , C12Q2600/156 , C40B40/06 , C40B60/14 , C12Q2565/513
摘要： The invention provides methods of comparing a target nucleic acid with a reference nucleic acid using nucleic acid arrays.
摘要翻译：本发明提供了使用核酸阵列比较靶核酸与参考核酸的方法。

8. 发明授权

US06284460B1 Hybridization and sequencing of nucleic acids using base pair mismatches 失效
标题翻译：使用碱基对错配的核酸的杂交和测序
公开(公告)号：US06284460B1
公开(公告)日：2001-09-04
申请号：US08877196
申请日：1997-06-17
申请人： Stephen P. A. Fodor , Robert J. Lipshutz , Xiaohua Huang
发明人： Stephen P. A. Fodor , Robert J. Lipshutz , Xiaohua Huang
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , B01J19/0046 , B01J2219/00432 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , C12Q2600/156 , C40B40/06 , C40B60/14 , G06F19/20 , C12Q2565/513 , C12Q2525/186 , C12Q2565/501 , C12Q2565/518 , C12Q2525/161 , C12Q2525/204
摘要： Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. Arrays of nucleic acids are formed by techniques, preferably high resolution, light-directed techniques. Positions of hybridization of a target nucleic acid are determined by, e.g., epifluorescence microscopy. Devices and techniques are proposed to determine the sequence of a target nucleic acid more efficiently and more quickly through such synthesis and detection techniques.
摘要翻译：用于核酸杂交和用于确定核酸序列的装置和技术。核酸序列通过技术，优选高分辨率，光导技术形成。通过例如落射荧光显微镜测定靶核酸的杂交位置。提出了设备和技术，以通过这种合成和检测技术更有效和更快地确定目标核酸的序列。

9. 发明授权

US06228593B1 Computer-aided probability base calling for arrays of nucleic acid probes on chips 有权
标题翻译：计算机辅助概率库，要求芯片上的核酸探针阵列
公开(公告)号：US06228593B1
公开(公告)日：2001-05-08
申请号：US09483190
申请日：2000-01-14
申请人： Robert J. Lipshutz , Michael G. Walker
发明人： Robert J. Lipshutz , Michael G. Walker
IPC分类号： C12Q168
CPC分类号： C12Q1/6837 , C12Q1/68 , G06F19/20 , Y10S435/973
摘要： A computer system for analyzing nucleic acid sequences is provided. The computer system is used to calculate probabilities for determining unknown bases by analyzing the fluorescence intensities of hybridized nucleic acid probes on biological chips. Additionally, information from multiple experiments is utilized to improve the accuracy of calling unknown bases.
摘要翻译：提供了用于分析核酸序列的计算机系统。计算机系统用于通过分析生物芯片上杂交的核酸探针的荧光强度来计算用于确定未知碱基的概率。另外，利用来自多个实验的信息来提高呼叫未知基数的准确性。

10. 发明申请

US20120178081A1 Methods of Labeling Cells, Labeled Cells, and uses Thereof 审中-公开
标题翻译：标记细胞的方法，标记细胞及其用途
公开(公告)号：US20120178081A1
公开(公告)日：2012-07-12
申请号：US13342912
申请日：2012-01-03
申请人： Quan N. Nguyen , Robert J. Lipshutz , Yunqing Ma
发明人： Quan N. Nguyen , Robert J. Lipshutz , Yunqing Ma
IPC分类号： G01N33/53
CPC分类号： G01N33/54313
摘要： Methods of detecting nucleic acids, proteins and cells including methods of detecting two or more nucleic acids, proteins and cells in multiplex bDNA assays, are provided. Assays may be conducted at least in vitro, in vivo, in cellulo, and in situ. Nucleic acids are detected, through cooperative hybridization that results in specific association of a label probe system with target nucleic acids. Embodiments are directed to concurrent detection of one or more nucleic acids and/or one or more proteins. The detected proteins may be intracellular or external markers on the surface of the cell. Detection of protein components is accomplished by use of specific antibodies and a label probe system and/or coated microparticles which bind to the outside surface of specific cells and contain specific probes that can be detected using the same label probe system. Compositions, kits, and systems related to the methods are also described.
摘要翻译：提供了检测核酸，蛋白质和细胞的方法，包括在多重bDNA测定中检测两种或更多种核酸，蛋白质和细胞的方法。测定可至少在体外，体内，纤维素和原位进行。通过协调杂交检测核酸，其导致标记探针系统与靶核酸的特异性结合。实施方案涉及同时检测一种或多种核酸和/或一种或多种蛋白质。检测到的蛋白质可以是细胞表面上的细胞内或外部标记。通过使用特异性抗体和标记探针系统和/或结合特定细胞的外表面并包含可以使用相同标记探针系统检测的特异性探针的包被的微粒来实现蛋白质组分的检测。还描述了与方法相关的组合物，试剂盒和系统。

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