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1. 发明授权

US5633129A Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices 失效
标题翻译：使用可替换的聚合物基质进行电泳检测和分离突变体DNA
公开(公告)号：US5633129A
公开(公告)日：1997-05-27
申请号：US190919
申请日：1994-02-03
申请人： Barry L. Karger , William G. Thilly , Frantisek Foret , Konstaintin Khrapko , Phouthone Koehavong , Aharon S. Cohen , Roger W. Giese
发明人： Barry L. Karger , William G. Thilly , Frantisek Foret , Konstaintin Khrapko , Phouthone Koehavong , Aharon S. Cohen , Roger W. Giese
IPC分类号： C12N15/09 , C12Q1/68 , G01N33/566
CPC分类号： C12Q1/6827 , C12Q1/6809 , Y10S435/81
摘要： The disclosure relates to a method for resolving double-stranded DNA species differing by at least one base pair. Each of the species is characterized by an iso-melting domain with a unique melting temperature contiguous with a melting domain of higher thermal stability.
摘要翻译：本公开涉及一种解决由至少一个碱基对不同的双链DNA种的方法。每个物种的特征在于具有与具有较高热稳定性的熔融区域相邻的独特熔融温度的等熔融区域。

2. 发明授权

US5089106A High performance capillary gel electrophoresis 失效
标题翻译：高性能毛细管凝胶电泳
公开(公告)号：US5089106A
公开(公告)日：1992-02-18
申请号：US649673
申请日：1991-02-01
申请人： Barry L. Karger , Roger W. Giese , Eva Szoko
发明人： Barry L. Karger , Roger W. Giese , Eva Szoko
IPC分类号： G01N27/447
CPC分类号： G01N27/44721 , G01N27/44747 , G01N27/44752
摘要： An improved, highly stable capillary column used in high performance capillary electrophoresis and method of preparing the same. The improved capillary column can significantly reduce the effects of electroosmosis during electrophoresis, is stable for reuse, and enables operation of the capillary column in high electric fields, resulting in high resolution separations in a minimum amount of time. The improved column comprises a capillary having a highly stable, coating matrix on the inner surface of the capillary wall. The highly stable coating matrix includes an intermediate layer hydrophobic phase chemically bonded to the inner wall of the capillary, a detergent which is physically adsorbed to the hydrophobic phase, and a polymerized monomer which is covalently bonded to the adsorbed detergent thereby forming a hydrophilic outer layer of the coating matrix.
摘要翻译：用于高效毛细管电泳的改进的，高度稳定的毛细管柱及其制备方法。改进的毛细管柱可以显着降低电泳过程中电渗的影响，对于重复使用是稳定的，并且能够在高电场下操作毛细管柱，从而在最短时间内产生高分辨率的分离。改进的柱包括在毛细管壁的内表面上具有高度稳定的涂层基质的毛细管。高度稳定的涂层基质包括化学键合到毛细管的内壁的中间层疏水相，物理吸附到疏水相的洗涤剂，和共价键合到吸附的洗涤剂上的聚合单体，从而形成亲水的外层的涂层基体。

3. 发明申请

US20100255607A1 AFFINITY CONTROL OF TAGGING NOISE 审中-公开
标题翻译：标签噪声的控制
公开(公告)号：US20100255607A1
公开(公告)日：2010-10-07
申请号：US12743462
申请日：2008-11-21
申请人： Roger W. Giese
发明人： Roger W. Giese
IPC分类号： G01N33/53
CPC分类号： G01N33/5306 , G01N33/537 , G01N33/58
摘要： Analytical methodology is disclosed in which a trace analyte in a sample is tagged to increase its signal strength for measurement, and an affinity substance is used at least after the tagging step (late in the method) to separate the tagged analyte from tagging noise, wherein the affinity substance is directed at the reactive part of the tag, at the reagent-reacted part of the tag, or at a nonreactive part of the analyte. This method includes the case where exposure of the sample to the affinity substance begins prior to and continues after the tagging reaction. The affinity substance comprises an affinity reagent such as an immobilized anti-body (immuno-affinity chromatography). This late use of an affinity substance (after the tagging reaction) is particularly important for methods in which trace analytes are reacted with a charge tag (tag possessing a charge) prior to detection by mass spectrometry.
摘要翻译：公开了分析方法，其中样品中的痕量分析物被标记以增加其用于测量的信号强度，并且至少在标记步骤（方法后期）使用亲和物质以将标记的分析物与标记噪声分离，其中亲和性物质指向标签的反应性部分，标签的试剂反应部分或分析物的非反应性部分。该方法包括样品暴露于亲和性物质之前开始标签反应之前和之后的情况。亲和性物质包括亲和试剂如固定化抗体（免疫亲和层析）。亲和性物质的后期使用（标记反应后）对于通过质谱法检测之前将痕量分析物与电荷标签（具有电荷的标签）反应的方法特别重要。

4. 发明申请

US20090242756A1 INTENSIFIED NEUTRAL LOSS TAGS AND USE THEREOF IN MASS SPECTROMETRY 有权
标题翻译：强化的中性损失标签及其在大量光谱中的应用
公开(公告)号：US20090242756A1
公开(公告)日：2009-10-01
申请号：US12479283
申请日：2009-06-05
申请人： Poguang Wang , Guodong Li , Jianxin Gao , Roger W. Giese , Xin Zhang
发明人： Poguang Wang , Guodong Li , Jianxin Gao , Roger W. Giese , Xin Zhang
IPC分类号： H01J49/00
CPC分类号： G01N33/6848 , Y10T436/143333 , Y10T436/145555 , Y10T436/24
摘要： Ultrasensitive detection of a chemical substance (analyte) by mass spectrometry is achieved by employing a molecular tag that yields an intense parent ion and then an intense daughter ion in a multi-stage mass spectrometer.
摘要翻译：通过质谱法对化学物质（分析物）进行超灵敏检测是通过使用在多级质谱仪中产生强亲本离子，然后产生强烈的子离子的分子标签来实现的。

5. 发明授权

US5512486A Single step signal group-imidazole labeling of organic phosphate groups under aqueous conditions 失效
标题翻译：在水性条件下单步信号组 - 有机磷酸酯基团的咪唑标记
公开(公告)号：US5512486A
公开(公告)日：1996-04-30
申请号：US60569
申请日：1993-05-10
申请人： Roger W. Giese , Poguang Wang
发明人： Roger W. Giese , Poguang Wang
IPC分类号： C07F5/02 , G01N33/533 , G01N33/48
CPC分类号： G01N33/533 , C07F5/022 , Y10T436/143333 , Y10T436/16 , Y10T436/163333
摘要： Compounds and methods for single step, covalent labeling of the phosphate group of an organic substance under aqueous conditions are described. The labeling compound includes any kind of detectable signal group covalently bound to an imidazole moiety, which can be imidazole or a substituted imidazole. A preferred labeling compound has the formula ##STR1##
摘要翻译：描述了在水性条件下单步，共价标记有机物质的磷酸酯基的化合物和方法。标记化合物包括与咪唑部分共价结合的任何种类的可检测信号基团，其可以是咪唑或取代的咪唑。优选的标记化合物具有式

6. 发明授权

US5650270A Molecular analytical release tags and their use in chemical analysis 失效
标题翻译：分子分析释放标签及其在化学分析中的应用
公开(公告)号：US5650270A
公开(公告)日：1997-07-22
申请号：US496251
申请日：1990-03-20
申请人： Roger W. Giese , Samy Abdel-Baky , Kariman Allam
发明人： Roger W. Giese , Samy Abdel-Baky , Kariman Allam
IPC分类号： G01N33/532 , C12Q1/68 , G01N31/00 , G01N33/53 , G01N33/561
CPC分类号： G01N33/532
摘要： Analytical reagents designated "release tags", for labeling molecular species with a highly detectable signal group which can be released in the form of a volatile compound at a desired point in an analytical procedure. In one embodiment, the release tags have the formula(SgCo).sub.x L(Rx).sub.rwherein each Sg is a signal group bearing one or more electronegative substituents, L is any of a wide variety of groups which when attached to a carbonyl group form a readily cleaved linkage, each COL moiety is a release group which upon scission releases signal group Sg in the form of a volative compound, and each Rx is a reactivity group for attaching the release tag compound to a molecular species to be labeled. In a second embodiment, the release tags have the formulaSgReRxwherein Sg and Rx are defined as above and Re is a release group which is an olefin, .alpha.-hydroxy ketone or vicinal diol. Conjugates of the release tag compounds and assay methods employing them are also disclosed.
摘要翻译：分析试剂被指定为“释放标签”，用于通过高度可检测的信号组来标记分子物质，其可以在分析程序中的所需点以挥发性化合物的形式释放。在一个实施方案中，释放标签具有式（SgCo）xL（Rx）r，其中每个Sg是带有一个或多个电负性取代基的信号基团，L是当连接到羰基上时易于形成的各种基团中的任何一种每个COL部分是释放基团，其在断裂时以挥发性化合物的形式释放信号基团Sg，并且每个Rx是用于将释放标签化合物连接到待标记的分子种类上的反应性基团。在第二实施方案中，释放标签具有式SgReRx，其中Sg和Rx如上所定义，Re是作为烯烃，α-羟基酮或连二醇的释放基团。还公开了释放标签化合物的缀合物和采用它们的测定方法。

7. 发明授权

US5516931A Release tag compounds producing ketone signal groups 失效
标题翻译：释放标签生成酮信号组的化合物
公开(公告)号：US5516931A
公开(公告)日：1996-05-14
申请号：US53608
申请日：1993-04-22
申请人： Roger W. Giese , Samy Abdel-Baky , Linxiao Xu
发明人： Roger W. Giese , Samy Abdel-Baky , Linxiao Xu
IPC分类号： C07D207/46 , G01N33/532 , G01N33/58 , G01N33/78 , C07C69/76
CPC分类号： G01N33/582 , C07D207/46 , G01N33/532 , G01N33/78
摘要： A release tag reagent suitable for use in the chemical analysis of a substance to be detected comprises signal, release, and reactivity groups. A class of release tag compounds that are cleaved to release as signal groups very stable electrophoric ketones which are sufficiently volatile for determination in the gas phase of an analytical reaction mixture is disclosed.
摘要翻译：适用于待检测物质的化学分析的释放标签试剂包括信号，释放和反应性基团。公开了一类释放标签化合物，其被切割以作为信号组释放非常稳定的电化学酮，其具有足够的挥发性以便在分析反应混合物的气相中测定。

8. 发明授权

US5445966A Extractive hydrogenation for chemical analyses 失效
标题翻译：萃取氢化用于化学分析
公开(公告)号：US5445966A
公开(公告)日：1995-08-29
申请号：US49278
申请日：1993-04-20
申请人： Roger W. Giese , Mohammad S. Itani
发明人： Roger W. Giese , Mohammad S. Itani
IPC分类号： G01N30/00 , G01N30/02 , G01N30/06 , G01N30/72
CPC分类号： G01N30/00 , G01N2030/025 , G01N2030/062 , G01N30/7206 , Y10T436/16 , Y10T436/20
摘要： A general purpose technique called extractive hydrogenation analysis, useful for both trace component and imaging analysis of samples containing organic matter. In processes according to the invention, organic matter is thoroughly decomposed in the presence of a hydrogenation catalyst under substantially aqueous conditions into products, and one or more of the products is then detected.
摘要翻译：一种通用技术，称为萃取氢化分析，可用于含有机物质样品的痕量成分和成像分析。在根据本发明的方法中，有机物在氢化催化剂存在下，在基本上含水条件下彻底分解成产物，然后检测一种或多种产物。

9. 发明授权

US5190864A Enzyme amplification by using free enzyme to release enzyme from an immobilized enzyme material 失效
标题翻译：通过使用游离酶从固定化酶材料释放酶的酶扩增
公开(公告)号：US5190864A
公开(公告)日：1993-03-02
申请号：US516321
申请日：1990-04-30
申请人： Roger W. Giese , Markus Ehrat , Douglas J. Cecchini
发明人： Roger W. Giese , Markus Ehrat , Douglas J. Cecchini
IPC分类号： C12Q1/00 , C12Q1/68
CPC分类号： C12N9/22 , C12Q1/00 , C12Q1/68
摘要： A method for amplifying enzyme activity is disclosed. Enzyme amplification is achieved by covalently bonding enzyme to a supporting material via a molecular chain which is a substrate for the enzyme, then introducing a small amount of enzyme in the free state to this system, causing release of a large amount of bound enzyme. In an alternative embodiment, complementary enzymatically inactive fragments of an active enzyme, which fragments can recombine to form active enzyme, are covalently attached to separate support materials by a molecular chain material which is a substrate for the active enzyme, and these two fragment-supported conjugates are connected in series. Upon application of free enzyme or free complementary enzyme to one of these fragment-support conjugates, followed by application of the resulting product mixture to the second fragment-support conjugate, a large amount of free enzyme is ultimately produced. In a second alternative embodiment, two different active enzymes are each attached to separate supporting materials by different leashes, in which the leash for the first enzyme only is cleaved in the system by the second enzyme, and the leash for the second enzyme only is cleaved in the system by the first enzyme. These two materials are connected in series, and upon application of the second enzyme to the first enzyme-support conjugate, followed by application of the released first enzyme to the second enzyme-support conjugate material, ultimately a large amount of released active second enzyme is produced. Also disclosed are molecular conjugates of enzyme material on supports, required for the enzyme amplification.
摘要翻译：公开了扩增酶活性的方法。酶扩增是通过将作为酶的底物的分子链共价键合到支持材料上，然后将少量酶以自由状态引入该系统，引起大量结合的酶的释放。在替代实施方案中，活性酶的互补的酶促无活性的片段可以重组以形成活性酶，通过作为活性酶底物的分子链材料共价连接到分离的载体材料上，并且这两个片段支持缀合物串联连接。当将游离酶或游离的互补酶应用于这些片段 - 支持的缀合物之一时，随后将所得的产物混合物施用到第二片段 - 支持缀合物上，最终产生大量的游离酶。在第二替代实施方案中，两种不同的活性酶各自通过不同的皮带连接到分离的支持材料，其中第一酶的皮带仅在系统中被第二酶切割，并且第二酶的皮带仅被切割在系统中由第一种酶。这两种材料是串联连接的，并且在将第二种酶施用于第一种酶 - 载体缀合物后，随后将释放的第一种酶施用于第二种酶 - 载体缀合物材料，最终大量释放的活性第二种酶是生产。还公开了在酶扩增所需的载体上的酶材料的分子缀合物。

10. 再颁专利

USRE31712E Biochemical avidin-biotin multiple-layer system 失效
标题翻译：生化抗生物素蛋白 - 生物素多层系统
公开(公告)号：USRE31712E
公开(公告)日：1984-10-23
申请号：US532037
申请日：1983-09-14
申请人： Roger W. Giese
发明人： Roger W. Giese
IPC分类号： A61L27/54 , A61L33/00 , G01N33/543
CPC分类号： B82Y30/00 , A61L27/54 , A61L33/0005 , G01N33/543 , Y10T428/2991 , Y10T428/2998 , Y10T428/31768
摘要： A multiple-layer process for applying, in alternate, successive layers, the protein, avidin, and a biotin-containing extender material to a solid surface to modify the properties of the surface and to the multiple-layer product so prepared.

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