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1. 发明授权

US5089106A High performance capillary gel electrophoresis 失效
标题翻译：高性能毛细管凝胶电泳
公开(公告)号：US5089106A
公开(公告)日：1992-02-18
申请号：US649673
申请日：1991-02-01
申请人： Barry L. Karger , Roger W. Giese , Eva Szoko
发明人： Barry L. Karger , Roger W. Giese , Eva Szoko
IPC分类号： G01N27/447
CPC分类号： G01N27/44721 , G01N27/44747 , G01N27/44752
摘要： An improved, highly stable capillary column used in high performance capillary electrophoresis and method of preparing the same. The improved capillary column can significantly reduce the effects of electroosmosis during electrophoresis, is stable for reuse, and enables operation of the capillary column in high electric fields, resulting in high resolution separations in a minimum amount of time. The improved column comprises a capillary having a highly stable, coating matrix on the inner surface of the capillary wall. The highly stable coating matrix includes an intermediate layer hydrophobic phase chemically bonded to the inner wall of the capillary, a detergent which is physically adsorbed to the hydrophobic phase, and a polymerized monomer which is covalently bonded to the adsorbed detergent thereby forming a hydrophilic outer layer of the coating matrix.
摘要翻译：用于高效毛细管电泳的改进的，高度稳定的毛细管柱及其制备方法。改进的毛细管柱可以显着降低电泳过程中电渗的影响，对于重复使用是稳定的，并且能够在高电场下操作毛细管柱，从而在最短时间内产生高分辨率的分离。改进的柱包括在毛细管壁的内表面上具有高度稳定的涂层基质的毛细管。高度稳定的涂层基质包括化学键合到毛细管的内壁的中间层疏水相，物理吸附到疏水相的洗涤剂，和共价键合到吸附的洗涤剂上的聚合单体，从而形成亲水的外层的涂层基体。

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