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1. 发明授权

US07604935B2 Soluble rubella E1 envelope protein variants 有权
标题翻译：可溶性风疹E1包膜蛋白变体
公开(公告)号：US07604935B2
公开(公告)日：2009-10-20
申请号：US11584887
申请日：2006-10-23
申请人： Barbara Upmeier , Ralf Bollhagen , Alfred Engel , Elke Faatz , Peter Schaarschmidt , Christian Scholz , Toralf Zarnt
发明人： Barbara Upmeier , Ralf Bollhagen , Alfred Engel , Elke Faatz , Peter Schaarschmidt , Christian Scholz , Toralf Zarnt
IPC分类号： C12Q1/70 , C12N7/01 , A61K39/20
CPC分类号： C07K14/005 , C07K2319/35 , C12N2770/36222 , Y10S530/826
摘要： A soluble rubella E1 antigen variant is disclosed that comprises amino acids 334-409 of the native rubella E1 peptide, but lacks the C-terminal end and at least the transmembrane region and the anchor segment as well as at least the amino acids 143 to 164. Also described is a recombinant DNA molecule encoding the rubella E1 antigen variants which are recombinantly expressed as a chaperone fusion protein, refolded into a soluble and immunoreactive conformation, and further used for the serological detection of anti-rubella antibodies. In addition, also disclosed is a method for the detection, determination and quantification of anti-rubella antibodies of IgG and/or IgM subclass in a sample wherein the rubella E1 antigen is used as a capture reagent and/or binding partner for the antibodies.
摘要翻译：公开了可溶性风疹E1抗原变体，其包含天然风疹E1肽的氨基酸334-409，但缺少C末端和至少跨膜区和锚定区以及至少氨基酸143至164 还描述了编码风疹E1抗原变体的重组DNA分子，其被重组表达为伴侣融合蛋白，重折叠成可溶性和免疫反应性构象，并进一步用于抗风疹抗体的血清学检测。此外，还公开了用于检测，测定和定量样品中IgG和/或IgM亚类的抗风疹抗体的方法，其中所述风疹E1抗原用作抗体的捕获试剂和/或结合配偶体。

2. 发明授权

US08551696B2 Rubella E1 envelope protein variants and their use in detection of anti-rubella antibodies 有权
标题翻译：风疹E1包膜蛋白变体及其在抗风疹抗体检测中的应用
公开(公告)号：US08551696B2
公开(公告)日：2013-10-08
申请号：US12793134
申请日：2010-06-03
申请人： Christian Scholz , Ralf Bollhagen , Alfred Engel , Elke Faatz , Peter Schaarschmidt , Barbara Upmeier , Toralf Zarnt
发明人： Christian Scholz , Ralf Bollhagen , Alfred Engel , Elke Faatz , Peter Schaarschmidt , Barbara Upmeier , Toralf Zarnt
IPC分类号： C12Q1/70 , A61K39/20 , C12N7/01
CPC分类号： G01N33/56983 , C07K14/005 , C07K2319/35 , C12N2770/36222 , G01N2333/19 , G01N2469/20
摘要： The invention relates to soluble rubella E1 antigens and variants of these antigens. The antigens contain amino acids 201 to 432 or 169 to 432 and are lacking amino acids 453 to 481 as well as at least the amino acids 143 to 164. They further contain a region spanning two disulfide-bridges. The invention also relates to a recombinant DNA molecule encoding the rubella E1 antigens, the expression of rubella E1 antigens as chaperone fusion proteins and their use in a method of detecting antibodies against rubella in a sample.
摘要翻译：本发明涉及可溶性风疹E1抗原和这些抗原的变体。抗原含有氨基酸201至432或169至432，并且缺少氨基酸453至481以及至少氨基酸143至164.它们还包含横跨两个二硫键的区域。本发明还涉及编码风疹E1抗原的重组DNA分子，风疹E1抗原作为伴侣融合蛋白的表达，以及它们在检测样品中风疹抗体的方法中的用途。

3. 发明申请

US20070154883A1 Soluble rubella E1 envelope protein variants 有权
标题翻译：可溶性风疹E1包膜蛋白变体
公开(公告)号：US20070154883A1
公开(公告)日：2007-07-05
申请号：US11584887
申请日：2006-10-23
申请人： Barbara Upmeier , Ralf Bollhagen , Alfred Engel , Elke Faatz , Peter Schaarschmidt , Christian Scholz , Toralf Zarnt
发明人： Barbara Upmeier , Ralf Bollhagen , Alfred Engel , Elke Faatz , Peter Schaarschmidt , Christian Scholz , Toralf Zarnt
IPC分类号： C12Q1/70
CPC分类号： C07K14/005 , C07K2319/35 , C12N2770/36222 , Y10S530/826
摘要： The invention disclosed relates to a soluble rubella E1 antigen and variants of this peptide characterized by lacking at the C-terminal end at least the transmembrane region and the anchor segment as well as at least the amino acids 143 to 164 and containing at least the region spanning the disulfide bridges Cys 349-Cys 352 and Cys 368-401 whereas the N-terminus (Cys 349) of this region contains additionally at least 15 amino acids and/or the C-terminus (Cys 401) of this region contains additionally at least 8 amino acids of the adjacent rubella E1 antigen sequence. Also described are a recombinant DNA molecule encoding the rubella E1 antigen and variants which are recombinantly expressed as a chaperone fusion protein, refolded into a soluble and immunoreactive conformation, and further used for the serological detection of anti-rubella antibodies. In addition, also disclosed is a method for the detection, determination and quantification of anti-rubella antibodies of IgG and/or IgM subclass in a sample wherein the rubella E1 antigen is used as a capture reagent and/or binding partner for the antibodies.
摘要翻译：所公开的本发明涉及可溶性风疹E1抗原和该肽的变体，其特征在于至少在C-末端缺少至少跨膜区和锚段以及至少氨基酸143至164并且至少含有该区域跨越二硫键Cys 349-Cys 352和Cys 368-401，而该区域的N末端（Cys 349）另外包含至少15个氨基酸和/或该区域的C末端（Cys 401）相邻风疹E1抗原序列的至少8个氨基酸。还描述了编码风疹E1抗原的重组DNA分子和重组表达为伴侣融合蛋白的变体，重新折叠成可溶性和免疫反应性构象，并进一步用于抗风疹抗体的血清学检测。此外，还公开了用于检测，测定和定量样品中IgG和/或IgM亚类的抗风疹抗体的方法，其中所述风疹E1抗原用作抗体的捕获试剂和/或结合配偶体。

4. 发明授权

US08426167B2 Methods for producing fusion polypeptides or enhancing expression of fusion polypeptides 有权
公开(公告)号：US08426167B2
公开(公告)日：2013-04-23
申请号：US11786847
申请日：2007-04-13
申请人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
发明人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
IPC分类号： C12P21/04
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
摘要： The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria, such as Escherichia coli. In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor; methods of recombinant protein expression, the recombinant polypeptides thus obtained, as well as to the use of such polypeptides.

5. 发明申请

US20090028893A1 Methods for producing fusion polypeptides or enhancing expression of fusion polypeptides 有权
标题翻译：用于产生融合多肽或增强融合多肽表达的方法
公开(公告)号：US20090028893A1
公开(公告)日：2009-01-29
申请号：US11786847
申请日：2007-04-13
申请人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
发明人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
IPC分类号： A61K39/00 , C12P21/02
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
摘要： The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria, such as Escherichia coli. In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor; methods of recombinant protein expression, the recombinant polypeptides thus obtained, as well as to the use of such polypeptides.
摘要翻译：本发明涉及在细菌如大肠杆菌中克隆和表达外源蛋白质或多肽。特别地，本发明涉及包含选自FkpA，SlyD和触发因子的FKBP型肽基脯氨酰异构酶的表达工具; 重组蛋白表达的方法，如此获得的重组多肽，以及这些多肽的用途。

6. 发明授权

US07244819B2 Fusion polypeptides, vaccines and compositions of FKBP chaperones and target polypeptides 有权
标题翻译：融合多肽，疫苗和FKBP伴侣和靶多肽的组合物
公开(公告)号：US07244819B2
公开(公告)日：2007-07-17
申请号：US10179038
申请日：2002-06-24
申请人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
发明人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
IPC分类号： C01K1/00
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
摘要： The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria, such as Escherichia coli. In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor; methods of recombinant protein expression, the recombinant polypeptides thus obtained, as well as to the use of such polypeptides.
摘要翻译：本发明涉及在细菌如大肠杆菌中克隆和表达外源蛋白质或多肽。特别地，本发明涉及包含选自FkpA，SlyD和触发因子的FKBP型肽基脯氨酰异构酶的表达工具; 重组蛋白表达的方法，如此获得的重组多肽，以及这些多肽的用途。

7. 发明授权

US08790659B2 SlpA as a tool for recombinant protein and enzyme technology 有权
标题翻译： SlpA作为重组蛋白和酶技术的工具
公开(公告)号：US08790659B2
公开(公告)日：2014-07-29
申请号：US13569353
申请日：2012-08-08
申请人： Christian Scholz , Elke Faatz , Urban Schmitt , Peter Schaarschmidt
发明人： Christian Scholz , Elke Faatz , Urban Schmitt , Peter Schaarschmidt
IPC分类号： A61K39/245
CPC分类号： C12N15/62 , C12N9/90 , G01N33/536 , G01N33/54393 , Y02A50/57
摘要： Disclosed are a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Also disclosed are a method for producing the fusion protein, a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein, and a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide.
摘要翻译：公开了编码包含SlpA伴侣和靶多肽的融合蛋白的重组DNA分子，其中人FK506结合蛋白（FKBP）被排除为靶多肽，编码所述融合蛋白的相应表达载体以及用所述表达载体转化的宿主细胞。还公开了产生融合蛋白的方法，重组产生的包含SlpA伴侣和靶多肽的融合蛋白。本发明的另一方面是使用重组产生的融合蛋白，以及含有重组产生的包含SlpA伴侣和靶多肽的融合蛋白的试剂盒。

8. 发明授权

US07094884B2 Soluble complexes of amylod β peptide and peptidyl prolyl isomerase chaperone and methods of making and using them 有权
标题翻译：淀粉状β肽和肽基脯氨酰异构酶伴侣的可溶性复合物及其制备和使用方法
公开(公告)号：US07094884B2
公开(公告)日：2006-08-22
申请号：US10443654
申请日：2003-05-22
申请人： Christian Scholz , Elke Faatz , Peter Schaarschmidt
发明人： Christian Scholz , Elke Faatz , Peter Schaarschmidt
IPC分类号： A61K38/24 , C07K14/00
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20 , G01N2800/2821 , Y02A50/472
摘要： The present invention relates to the diagnosis of Alzheimer's Disease. It especially teaches the production of a soluble Aβ-chaperone complex and the advantageous use of such chaperone-Aβ complex, especially in the detection of Aβ in an immunoassay, as well as its use as an immunogen.
摘要翻译：本发明涉及阿尔茨海默病的诊断。它特别教导了可溶性的Abeta-伴侣复合物的生产和这种伴侣 - Aβ复合物的有利使用，特别是在免疫测定中检测Abeta以及其作为免疫原的用途。

9. 发明申请

US20050112720A1 SOLUBLE COMPLEXES OF AMYLOD BETA PEPTIDE AND PEPTIDYL PROLYL ISOMERASE CHAPERONE AND METHODS OF MAKING AND USING THEM 有权
标题翻译：氨基酸肽和磷脂酰胆碱异构酶的可溶性复合物及其制备和使用方法
公开(公告)号：US20050112720A1
公开(公告)日：2005-05-26
申请号：US10443654
申请日：2003-05-22
申请人： Christian Scholz , Elke Faatz , Peter Schaarschmidt
发明人： Christian Scholz , Elke Faatz , Peter Schaarschmidt
IPC分类号： A61K39/00 , A61K47/48 , C07K14/16 , C12N9/90 , G01N33/543 , G01N33/569 , C12P21/06
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20 , G01N2800/2821 , Y02A50/472
摘要： The present invention relates to the diagnosis of Alzheimer's Disease. It especially teaches the production of a soluble Aβ-chaperone complex and the advantageous use of such chaperone-Aβ complex, especially in the detection of Aβ in an immunoassay, as well as its use as an immunogen.
摘要翻译：本发明涉及阿尔茨海默病的诊断。它特别教导了可溶性的Abeta-伴侣复合物的生产和这种伴侣 - Aβ复合物的有利使用，特别是在免疫测定中检测Abeta以及其作为免疫原的用途。

10. 发明授权

US07947494B2 Fusion protein comprising an E. coli chaperone protein and a human chaperone protein 有权
标题翻译：包含大肠杆菌伴侣蛋白和人伴侣蛋白的融合蛋白
公开(公告)号：US07947494B2
公开(公告)日：2011-05-24
申请号：US12166574
申请日：2008-07-02
申请人： Christian Scholz , Elke Faatz , Peter Schaarschmidt , Urban Schmitt
发明人： Christian Scholz , Elke Faatz , Peter Schaarschmidt , Urban Schmitt
IPC分类号： C12N15/00 , C07H21/04
CPC分类号： C12N9/90 , C07K2319/35
摘要： The invention discloses the cloning, expression and uses of a chimeric fusion protein with superior chaperone and folding activities compared to the wild type chaperones. This invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule comprising nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for an FK506 binding protein (FKBP) or an FK506-binding-protein-like domain (FKBP-like domain). In particular, this invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule comprising nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for a human FKBP type peptidyl-prolyl-cis/trans isomerase (PPIase), methods of producing these chimeric fusion proteins and their uses as folding helpers in the production of other proteins and in the process of the production of vaccines or pharmaceuticals, and as folding helpers for performing immunoassays.
摘要翻译：本发明公开了与野生型伴侣相比，具有优异的伴侣和折叠活性的嵌合融合蛋白的克隆，表达和用途。本发明涉及由重组DNA分子编码的嵌合融合蛋白，其包含编码非人伴侣蛋白多肽结合片段的核苷酸序列和编码FK506结合蛋白（FKBP）或FK506-结合蛋白 - 像域（FKBP样域）。特别地，本发明涉及由重组DNA分子编码的嵌合融合蛋白，其包含编码非人伴侣蛋白多肽结合片段的核苷酸序列和编码人FKBP型肽基 - 脯氨酰 - 顺/反异构酶的核苷酸序列（PPIase），生产这些嵌合融合蛋白的方法及其在生产其他蛋白质和疫苗或药物生产过程中作为折叠助剂的用途，以及用于进行免疫测定的折叠助剂。

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