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1. 发明申请

WO2005080545A2 VERFAHREN ZUR HERSTELLUNG VON REKOMBINANTEN PROTEINEN IN EUKARYONTISCHEN WIRTSZELLEN 审中-公开
标题翻译：在真核细胞宿主细胞中生产重组蛋白质的方法
公开(公告)号：WO2005080545A2
公开(公告)日：2005-09-01
申请号：PCT/EP2005/001575
申请日：2005-02-16
申请人： BIOCEUTICALS ARZNEIMITTEL AG , HAKEMEYER, Christian , LÜTKEMEYER, Dirk , LEHMANN, Jürgen
发明人： HAKEMEYER, Christian , LÜTKEMEYER, Dirk , LEHMANN, Jürgen
IPC分类号： C12M1/00
CPC分类号： C07K14/505 , C12M41/00 , C12P21/00
摘要： Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung von rekombinanten Proteinen in einer eukaryontischen Wirtszelllinie, das dadurch gekennzeichnet ist, dass die Kultivierung der Wirtszellen unter kontinuierlicher Zufütterung von Nährstoffen erfolgt, wobei die Geschwindigkeit der Zufütterung in linearer Abhängigkeit von der Dauer der Zufütterung erhöht wird.
摘要翻译：
本发明涉及一种用于在真核宿主细胞系中的重组蛋白的生产，其特征在于在N BEAR的连续Zuf导航用途SHAKE进行hrstoffen宿主细胞的培养的方法，其中的Zuf导航使用的速度是根据饲料持续时间的线性依赖性获得的。

2. 发明授权

EP1716225B1 VERFAHREN ZUR HERSTELLUNG VON REKOMBINANTEN PROTEINEN IN EUKARYONTISCHEN WIRTSZELLEN 无效
标题翻译：过程在真核宿主细胞中生产重组蛋白的
公开(公告)号：EP1716225B1
公开(公告)日：2007-11-14
申请号：EP05715356.1
申请日：2005-02-16
申请人： Bioceuticals Arzneimittel AG
发明人： HAKEMEYER, Christian , LÜTKEMEYER, Dirk , LEHMANN, Jürgen
IPC分类号： C12M1/00 , C12M3/00 , C12P21/00 , C12N5/00 , C07K14/505
CPC分类号： C07K14/505 , C12M41/00 , C12P21/00
摘要： The invention relates to a method for the production of recombined proteins in a eukaryontic host cell line. The invention is characterised in that the host cells are cultivated by continuously feeding nutrients and the speed of feeding is increased in a linear manner according to the duration of feeding.

3. 发明公开

EP1716225A2 VERFAHREN ZUR HERSTELLUNG VON REKOMBINANTEN PROTEINEN IN EUKARYONTISCHEN WIRTSZELLEN 无效
标题翻译：过程在真核宿主细胞中生产重组蛋白的
公开(公告)号：EP1716225A2
公开(公告)日：2006-11-02
申请号：EP05715356.1
申请日：2005-02-16
申请人： Bioceuticals Arzneimittel AG
发明人： HAKEMEYER, Christian , LÜTKEMEYER, Dirk , LEHMANN, Jürgen
IPC分类号： C12M1/00 , C12M3/00 , C12P21/00 , C12N5/00 , C07K14/505
CPC分类号： C07K14/505 , C12M41/00 , C12P21/00
摘要： The invention relates to a method for the production of recombined proteins in a eukaryontic host cell line. The invention is characterised in that the host cells are cultivated by continuously feeding nutrients and the speed of feeding is increased in a linear manner according to the duration of feeding.

4. 发明申请

WO2012059520A1 SPECTROSCOPIC FINGER-PRINTING OF RAW MATERIALS 审中-公开
标题翻译：原料的光谱手指打印
公开(公告)号：WO2012059520A1
公开(公告)日：2012-05-10
申请号：PCT/EP2011/069267
申请日：2011-11-03
申请人： F. HOFFMANN-LA ROCHE AG , CARDOSO-MENEZES, José , HAKEMEYER, Christian , JOSE, Gledson Emidio , STRAUSS, Ulrike , WERZ, Silke
发明人： CARDOSO-MENEZES, José , HAKEMEYER, Christian , JOSE, Gledson Emidio , STRAUSS, Ulrike , WERZ, Silke
IPC分类号： G01N21/35 , G01N33/68 , C12M1/34 , C12M3/00 , G01N21/64 , G01J3/44
CPC分类号： G01N21/78 , G01N21/3577 , G01N21/359 , G01N21/6486 , G01N24/087 , G01N2021/6423 , G01N2201/1293
摘要： Herein is reported a method for the selection of cultivation component batches to be used in the cultivation of a mammalian cell expressing a protein of interest wherein at least two different components are employed in the cultivation comprising the following steps: a) providing spectra of different batches of a first component obtained with a first spectroscopic method and spectra of a second component obtained with a second different spectroscopic method and the cultivation supernatant yield of the protein of interest obtained in a cultivation using combinations of these different batches of the first and the second component, b) processing the spectra, filtering the spectra, smoothing the spectra, and transforming the spectra to their first derivative, c) identifying patterns in the spectra, d) identifying a relation of the patterns identified in d) with the yield of the cultivation, e) providing a spectrum of a further batch of the first component obtained with the first spectroscopic method and a spectrum of a further batch of the second component obtained with the second spectroscopic method, f) processing the spectra, filtering the spectra, smoothing the spectra, and transforming the spectra to their first derivative, g) selecting the combination of the provided first component and the provided second component if the predicted cultivation supernatant yield based on the relation identified in d) is within +/- 10 % of the mean yield provided in a).
摘要翻译：本文报道了选择用于培养表达目的蛋白质的哺乳动物细胞培养组分批次的方法，其中在培养中使用至少两种不同的组分，其包括以下步骤：a）提供不同批次的光谱使用第一光谱法获得的第一组分和用第二种不同分光光度法获得的第二组分的光谱和使用这些不同批次的第一和第二组分的组合在培养中获得的目标蛋白的培养上清液产率，b）处理光谱，过滤光谱，平滑光谱，以及将光谱转换为其一阶导数，c）识别光谱中的图案，d）确定d）中鉴定的图案与培养产量的关系 e）提供用第一光谱获得的另一批第一组分的光谱 opic方法和用第二光谱法获得的另一批第二组分的光谱，f）处理光谱，过滤光谱，平滑光谱，以及将光谱转换成其一阶导数，g）选择提供第一组分和所提供的第二组分，如果基于在d）中鉴定的关系的预测培养上清液产量在a）中提供的平均产量的+/- 10％内。

5. 发明申请

WO2011110598A1 METHOD FOR PURIFYING IMMUNOGLOBULIN SOLUTIONS 审中-公开
标题翻译：纯化免疫球蛋白溶液的方法
公开(公告)号：WO2011110598A1
公开(公告)日：2011-09-15
申请号：PCT/EP2011/053547
申请日：2011-03-09
申请人： F. HOFFMANN-LA ROCHE AG , BINDER, Vinzenz , HAKEMEYER, Christian , SCHWARZ, Felizitas
发明人： BINDER, Vinzenz , HAKEMEYER, Christian , SCHWARZ, Felizitas
IPC分类号： C07K1/30 , C07K16/18
CPC分类号： C07K16/00 , C07K1/30
摘要： Herein is reported a method for purifying cell cultivation supernatants either directly after fermentation or after one or more preliminary purification steps, such as protein A affinity chromatography. By adjusting the pH value in the acid range and subsequent incubation of the acidified solution host cell nucleic acid and host cell protein can be precipitated but the target polypeptide remains in solution. Thereafter the precipitate and therewith the contaminating host cell components can be removed by a simple physical separation step.
摘要翻译：本文报道了直接在发酵后或在一个或多个初步纯化步骤如蛋白A亲和层析之后纯化细胞培养上清液的方法。通过调节酸值范围内的pH值，随后孵育酸化的溶液宿主细胞核酸和宿主细胞蛋白质可以沉淀，但目标多肽保留在溶液中。此后，可以通过简单的物理分离步骤除去沉淀物和污染的宿主细胞成分。

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