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1. 发明申请

WO2012059520A1 SPECTROSCOPIC FINGER-PRINTING OF RAW MATERIALS 审中-公开
标题翻译：原料的光谱手指打印
公开(公告)号：WO2012059520A1
公开(公告)日：2012-05-10
申请号：PCT/EP2011/069267
申请日：2011-11-03
申请人： F. HOFFMANN-LA ROCHE AG , CARDOSO-MENEZES, José , HAKEMEYER, Christian , JOSE, Gledson Emidio , STRAUSS, Ulrike , WERZ, Silke
发明人： CARDOSO-MENEZES, José , HAKEMEYER, Christian , JOSE, Gledson Emidio , STRAUSS, Ulrike , WERZ, Silke
IPC分类号： G01N21/35 , G01N33/68 , C12M1/34 , C12M3/00 , G01N21/64 , G01J3/44
CPC分类号： G01N21/78 , G01N21/3577 , G01N21/359 , G01N21/6486 , G01N24/087 , G01N2021/6423 , G01N2201/1293
摘要： Herein is reported a method for the selection of cultivation component batches to be used in the cultivation of a mammalian cell expressing a protein of interest wherein at least two different components are employed in the cultivation comprising the following steps: a) providing spectra of different batches of a first component obtained with a first spectroscopic method and spectra of a second component obtained with a second different spectroscopic method and the cultivation supernatant yield of the protein of interest obtained in a cultivation using combinations of these different batches of the first and the second component, b) processing the spectra, filtering the spectra, smoothing the spectra, and transforming the spectra to their first derivative, c) identifying patterns in the spectra, d) identifying a relation of the patterns identified in d) with the yield of the cultivation, e) providing a spectrum of a further batch of the first component obtained with the first spectroscopic method and a spectrum of a further batch of the second component obtained with the second spectroscopic method, f) processing the spectra, filtering the spectra, smoothing the spectra, and transforming the spectra to their first derivative, g) selecting the combination of the provided first component and the provided second component if the predicted cultivation supernatant yield based on the relation identified in d) is within +/- 10 % of the mean yield provided in a).
摘要翻译：本文报道了选择用于培养表达目的蛋白质的哺乳动物细胞培养组分批次的方法，其中在培养中使用至少两种不同的组分，其包括以下步骤：a）提供不同批次的光谱使用第一光谱法获得的第一组分和用第二种不同分光光度法获得的第二组分的光谱和使用这些不同批次的第一和第二组分的组合在培养中获得的目标蛋白的培养上清液产率，b）处理光谱，过滤光谱，平滑光谱，以及将光谱转换为其一阶导数，c）识别光谱中的图案，d）确定d）中鉴定的图案与培养产量的关系 e）提供用第一光谱获得的另一批第一组分的光谱 opic方法和用第二光谱法获得的另一批第二组分的光谱，f）处理光谱，过滤光谱，平滑光谱，以及将光谱转换成其一阶导数，g）选择提供第一组分和所提供的第二组分，如果基于在d）中鉴定的关系的预测培养上清液产量在a）中提供的平均产量的+/- 10％内。

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