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1. 发明申请

WO2004065623A3 ASSAY FOR INHIBITORS OF ISPH 审中-公开
标题翻译： ISPH抑制剂测定
公开(公告)号：WO2004065623A3
公开(公告)日：2004-09-30
申请号：PCT/EP2004000264
申请日：2004-01-15
申请人： BACHER ADELBERT , ADAM PETRA , EISENREICH WOLFGANG , GRAEWERT TOBIAS , HECHT STEFAN , ROHDICH FELIX , ZEPECK FERDINAND
发明人： BACHER ADELBERT , ADAM PETRA , EISENREICH WOLFGANG , GRAEWERT TOBIAS , HECHT STEFAN , ROHDICH FELIX , ZEPECK FERDINAND
IPC分类号： C12Q1/26
CPC分类号： C12Q1/26
摘要： Assay for testing a sample for the presence or absence of inhibition of the enzymatic conversion of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate into isopentenyl diphosphate and/or dimethylallyl diphosphate by the following steps: a) reacting an aqueous mixture containing 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate, a 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate reductase, and a reducing agent under predetermined reaction conditions for a predetermined period of time; b) analyzing the reaction mixture obtained in step (a) for the consumed amount of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate and/or NAD(P)H and/or for the produced amount of isopentenyl diphosphate, and/or dimethylallyl diphosphate and/or NAD(P) ; c) repeating step (a) in the presence of the sample to be tested; d) repeating step (b) with the reaction mixture defined in step (c); e) comparing the results of steps (b) and (d).
摘要翻译：用于通过以下步骤测试样品中是否存在抑制1-羟基-2-甲基 - （E） - 丁基-4-二磷酸酯转化成异戊烯基二磷酸酯和/或二甲基烯丙基二磷酸酯的步骤：a）使含有1-羟基-2-甲基 - （E） - 丁基-4-二磷酸酯，1-羟基-2-甲基 - （E） - 丁基-4-二磷酸还原酶的水性混合物和还原剂，在预定的反应条件下预定一段的时间; b）分析步骤（a）中获得的反应混合物中消耗量的4-羟基-2-甲基 - （E） - 丁基-4-二磷酸酯和/或NAD（P）H和/或产生的异戊烯基二磷酸酯和/或二甲基烯丙基二磷酸酯和/或NAD（P）+; c）在待测试样品存在下重复步骤（a）; d）用步骤（c）中定义的反应混合物重复步骤（b）; e）比较步骤（b）和（d）的结果。

2. 发明申请

WO0194561A3 THE NON-MEVALONATE ISOPRENOID PATHWAY 审中-公开
标题翻译：非诺贝特伊斯兰布尔途径
公开(公告)号：WO0194561A3
公开(公告)日：2002-05-30
申请号：PCT/EP0106255
申请日：2001-06-01
申请人： BACHER ADELBERT , ZENK MEINHART H , ADAM PETRA , EISENREICH WOLFGANG , FELLERMEIER MONIKA , HECHT STEFAN , ROHDICH FELIX , SCHUHR CHRISTOPH A , WUNGSINTAWEEKUL JURAITHIP
发明人： BACHER ADELBERT , ZENK MEINHART H , ADAM PETRA , EISENREICH WOLFGANG , FELLERMEIER MONIKA , HECHT STEFAN , ROHDICH FELIX , SCHUHR CHRISTOPH A , WUNGSINTAWEEKUL JURAITHIP
IPC分类号： C12N9/00 , C12N15/52 , C12N15/82 , C12P9/00 , A01H5/10
CPC分类号： C12N9/00 , C12N15/52 , C12N15/8274 , C12P9/00
摘要： The present invention is directed to enzymes and intermediates of the non-mevalonate isoprenoid pathway downstream of 2C-methyl-D-erythritol 2,4-cyclopyrophosphate and upstream of isopentenyl pyrophosphate or dimethylallyl pyrophosphate. These are used as a basis for a screening method for inhibitors of these enzymes, and a method for identifying inhibitor-resistant variants thereof. Further disclosures refer to DNA coding for said enzymes and for inhibitor-resistant variants thereof, vectors containing said DNA, cells containing said vector, and plant seeds comprising cells containing said vector. This invention is useful for the inhibition of the biosynthesis of isoprenoids in plants, bacteria and protozoa, for confering herbicide-resistance to plants, as well as for weed control in agriculture using a crop containing a herbicide-resistant gene and an effective amount of a suitable herbicide.
摘要翻译：本发明涉及2-甲基-D-赤藓糖醇2,4-环丙基磷酸酯和异戊烯基焦磷酸或二甲基烯丙基焦磷酸的上游的非甲羟戊酸异戊二烯通路的酶和中间体。这些用作这些酶的抑制剂的筛选方法的基础，以及用于鉴定其抑制剂抗性变体的方法。还涉及编码所述酶和其抑制剂抗性变体的DNA，含有所述DNA的载体，含有所述载体的细胞，以及含有所述载体的细胞的植物种子。本发明可用于抑制植物，细菌和原生动物中的类异戊二烯的生物合成，以赋予对植物的除草剂抗性，以及在使用含有除草剂抗性基因的作物和有效量的合适的除草剂。

3. 发明专利

DE602004003334D1 ASSAY FÜR ISPH-INHIBITOREN 未知
公开(公告)号：DE602004003334D1
公开(公告)日：2007-01-04
申请号：DE602004003334
申请日：2004-01-15
申请人： BACHER ADELBERT
发明人： BACHER ADELBERT , ADAM PETRA , EISENREICH WOLFGANG , GRAEWERT TOBIAS , HECHT STEFAN , ROHDICH FELIX , ZEPECK FERDINAND
IPC分类号： C12Q1/26
摘要： Assay for testing a sample for the presence or absence of inhibition of the enzymatic conversion of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate into isopentenyl diphosphate and/or dimethylallyl diphosphate by the following steps: (a) reacting an aqueous mixture containing 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate, a 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphnte reductase, and a reducing agent under predetermined reaction conditions for a predetermined period of time; (b) analyzing the reaction mixture obtained in step (a) for the consumed amount of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate and/or NAD(P)H and/or for the produced amount of isopentenyl diphosphate, and/or dimethylallyl diphosphate and/or NAD(P) + ; (c) repeating step (a) in the presence of the sample to be tested; (d) repeating step (b) with the reaction mixture defined in step (c); (e) comparing the results of steps (b) and (d).

4. 发明专利

DE10027821A1 New intermediate in isoprenoid biosynthesis, useful in screening for potential herbicides, comprises mutant encoding-enzymes sequences for imparting herbicide resistance 未知
公开(公告)号：DE10027821A1
公开(公告)日：2001-12-06
申请号：DE10027821
申请日：2000-06-05
申请人： BACHER ADELBERT , ZENK MEINHART
发明人： ADAM PETRA , BACHER ADELBERT , EISENREICH WOLFGANG , FELLERMEIER MONIKA , HECHT STEFAN , ROHDICH FELIX , SCHUHR CHRISTOPH A , WUNGSINTAWEEKUL JURAITHIP , ZENK MEINHART H
IPC分类号： C12N9/00 , C12N15/52 , C12N15/82 , C12P9/00 , C12N15/63 , C12N5/10 , C07H19/207 , C07H11/04 , C07H1/02 , C07B59/00 , A01N57/10
摘要： Intermediate (A), optionally isotopically labeled, in the mevalonate-independent pathway of isoprenoid biosynthesis, is new. Independent claims are also included for the following: (a) aqueous cell fluid containing (I) and (A), with at least one present at higher concentration than in fluids from normal cells or organisms; (b) preparing (A); (c) enzymes (B) that convert (I) to (A); (d) isolated, purified nucleic acid (C) encoding (B); (e) DNA vectors containing (C); (f) recombinant cells containing the vector of (e); (g) screening chemical libraries for inhibitors of isoprenoid biosynthesis based on blocking conversion of (I); (h) identifying inhibitor-resistant variants of (B); (i) (B) variants identified by method (h); (j) nucleic acid (C') encoding the variants of (f); (k) (l) vectors containing (C'); (l) cells containing the vectors of (l); (m) imparting herbicide resistance to plants by incorporation of (C'); (n) weed control that involves growing crop plants of (n); (o) inhibitors (D) identified by method (g); (p) composition for inhibiting biosynthesis of isoprenoids containing (D); and (q) inhibiting biosynthesis of isoprenoids by treatment with (D) or the compositions of (q).

5. 发明申请

WO02092800A3 CRYSTAL STRUCTURE OF 2C-METHYL-D-ERYTHRITOL 2,4-CYCLODIPHOSPHATE SYNTHASE 审中-公开
标题翻译： 2-甲基-D-赖氨酸2,4-环己基磷酸酯合成酶的晶体结构
公开(公告)号：WO02092800A3
公开(公告)日：2003-08-21
申请号：PCT/EP0205238
申请日：2002-05-13
申请人： BACHER ADELBERT , MAX PLANCK GESELLSCHAFT , HECHT STEFAN , HUBER ROBERT , KAISER JOHANNES , ROHDICH FELIX , STEINBACHER STEFAN
发明人： BACHER ADELBERT , HECHT STEFAN , HUBER ROBERT , KAISER JOHANNES , ROHDICH FELIX , STEINBACHER STEFAN
IPC分类号： C12N9/88 , G06F17/50 , G06F19/00
CPC分类号： C12Y406/01012 , C07K2299/00 , C12N9/88
摘要： This invention discloses the crystal structure of 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase and crystal structures of said synthase with 4-diphosphocytidyl-2C-methyl-D-erythritol, 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate, cytidine monophosphate, cytidine diphosphate, cytidine and a combination of cytidine monophosphate and 2C-methyl-D-erythritol 2,4-cyclodiphosphate; with or without zinc. Further, computer-aided methods of identifying inhibitors of said synthase and inhibitors are provided.
摘要翻译：本发明公开了2-甲基-D-赤藓糖醇2,4-环二磷酸合成酶的晶体结构以及所述合成酶与4-二磷酸二巯基-2C-甲基-D-赤藓糖醇，4-二磷酸胞苷-2C-甲基-D-赤藓糖醇的晶体结构二磷酸胞苷，胞苷二磷酸，胞苷和胞苷单磷酸和2-甲基-D-赤藓糖醇2,4-环磷酸二酯的组合; 有或没有锌。此外，提供了鉴定所述合成酶和抑制剂的抑制剂的计算机辅助方法。

6. 发明申请

WO02083720A2 INTERMEDIATES AND ENZYMES OF THE NON-MEVALONATE ISOPRENOID PATHWAY 审中-公开
标题翻译：非甲酸异丙酯途径的中间体和酶
公开(公告)号：WO02083720A2
公开(公告)日：2002-10-24
申请号：PCT/EP0204005
申请日：2002-04-10
申请人： BACHER ADELBERT , ROHDICH FELIX , ADAM PETRA , AMSLINGER SABINE , EISENREICH WOLFGANG , HECHT STEFAN
发明人： BACHER ADELBERT , ROHDICH FELIX , ADAM PETRA , AMSLINGER SABINE , EISENREICH WOLFGANG , HECHT STEFAN
IPC分类号： G01N33/50 , A61K31/663 , A61P31/04 , A61P37/02 , A61P37/04 , A61P43/00 , C07B61/00 , C07C33/025 , C07C33/035 , C07C47/263 , C07F9/09 , C07F9/113 , C07H19/10 , C07K16/44 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N9/16 , C12N9/88 , C12N9/99 , C12N15/09 , C12N15/52 , C12N15/55 , C12P9/00 , C12Q1/02 , G01N33/15 , G01N33/569 , C07K14/00
CPC分类号： C07C33/025 , C07B2200/05 , C07F9/098 , C07F9/113 , C07H19/10 , C12N15/52 , C12P9/00 , C40B40/00 , G01N2500/04
摘要： The invention provides a protein in a form that is functional for the enzymatic conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate to 1-hydroxy-2-methyl-2-butenyl 4-diphosphate notably in its (E)-form of the non-mevalonate biosynthetic pathway to isoprenoids. The invention also provides a protein in a form that is functional for the enzymatic conversion of 1-hydroxy-2-methyl-2butenyl 4-diphosphate, notably in its (E)-form, to isopentenyl diphosphate and/or dimethylallyl diphosphate. Further, screening methods for inhibitors of these proteins are provided. Further, 1-hydroxy-2-methyl-2-butenyl 4-diphosphate is provided and chemical and enzymatic methods of its preparation.
摘要翻译：本发明提供了一种形式的蛋白质，其功能是将2-甲基-D-赤藓糖醇2,4-环磷酸酯酶催化转化成1-羟基-2-甲基-2-丁烯基4-二磷酸酯，特别是其（E） - 形式的非甲羟戊酸生物合成途径到类异戊二烯。本发明还提供了一种形式的蛋白质，其特征在于其（E） - 形式的1-羟基-2-甲基 - 二烯丙基二磷酸酯的酶促转化为异戊烯基二磷酸酯和/或二甲基烯丙基二磷酸酯。此外，提供了这些蛋白质的抑制剂的筛选方法。此外，提供1-羟基-2-甲基-2-丁烯基4-二磷酸酯及其制备的化学和酶法。

8. 发明专利

AU7408401A The non-mevalonate isoprenoid pathway 未知
公开(公告)号：AU7408401A
公开(公告)日：2001-12-17
申请号：AU7408401
申请日：2001-06-01
申请人： BACHER ADELBERT , ZENK MEINHART H
发明人： BACHER ADELBERT , ZENK MEINHART H , ADAM PETRA , EISENREICH WOLFGANG , FELLERMEIER MONIKA , HECHT STEFAN , ROHDICH FELIX , SCHUHR CHRISTOPH A , WUNGSINTAWEEKUL JURAITHIP
IPC分类号： C12N9/00 , C12N15/52 , C12N15/82 , C12P9/00
摘要： Intermediate (A), optionally isotopically labeled, in the mevalonate-independent pathway of isoprenoid biosynthesis, is new. Independent claims are also included for the following: (a) aqueous cell fluid containing (I) and (A), with at least one present at higher concentration than in fluids from normal cells or organisms; (b) preparing (A); (c) enzymes (B) that convert (I) to (A); (d) isolated, purified nucleic acid (C) encoding (B); (e) DNA vectors containing (C); (f) recombinant cells containing the vector of (e); (g) screening chemical libraries for inhibitors of isoprenoid biosynthesis based on blocking conversion of (I); (h) identifying inhibitor-resistant variants of (B); (i) (B) variants identified by method (h); (j) nucleic acid (C') encoding the variants of (f); (k) (l) vectors containing (C'); (l) cells containing the vectors of (l); (m) imparting herbicide resistance to plants by incorporation of (C'); (n) weed control that involves growing crop plants of (n); (o) inhibitors (D) identified by method (g); (p) composition for inhibiting biosynthesis of isoprenoids containing (D); and (q) inhibiting biosynthesis of isoprenoids by treatment with (D) or the compositions of (q).

9. 发明专利

AT415479T 未知
公开(公告)号：AT415479T
公开(公告)日：2008-12-15
申请号：AT01940547
申请日：2001-06-01
申请人： BACHER ADELBERT , ZENK MEINHART H
发明人： BACHER ADELBERT , ZENK MEINHART , ADAM PETRA , EISENREICH WOLFGANG , FELLERMEIER MONIKA , HECHT STEFAN , ROHDICH FELIX , SCHUHR CHRISTOPH , WUNGSINTAWEEKUL JURAITHIP
IPC分类号： C12N15/52 , A01H5/10 , C12N9/00 , C12N15/82 , C12P9/00
摘要： Intermediate (A), optionally isotopically labeled, in the mevalonate-independent pathway of isoprenoid biosynthesis, is new. Independent claims are also included for the following: (a) aqueous cell fluid containing (I) and (A), with at least one present at higher concentration than in fluids from normal cells or organisms; (b) preparing (A); (c) enzymes (B) that convert (I) to (A); (d) isolated, purified nucleic acid (C) encoding (B); (e) DNA vectors containing (C); (f) recombinant cells containing the vector of (e); (g) screening chemical libraries for inhibitors of isoprenoid biosynthesis based on blocking conversion of (I); (h) identifying inhibitor-resistant variants of (B); (i) (B) variants identified by method (h); (j) nucleic acid (C') encoding the variants of (f); (k) (l) vectors containing (C'); (l) cells containing the vectors of (l); (m) imparting herbicide resistance to plants by incorporation of (C'); (n) weed control that involves growing crop plants of (n); (o) inhibitors (D) identified by method (g); (p) composition for inhibiting biosynthesis of isoprenoids containing (D); and (q) inhibiting biosynthesis of isoprenoids by treatment with (D) or the compositions of (q).

10. 发明专利

DE10302370A1 未知
公开(公告)号：DE10302370A1
公开(公告)日：2004-08-05
申请号：DE10302370
申请日：2003-01-22
申请人： BACHER ADELBERT
发明人： ADAM PETRA , BACHER ADELBERT , EISENREICH WOLFGANG , GRAEWERT TOBIAS , HECHT STEFAN , ROHDICH FELIX , ZEPECK FERDINAND
IPC分类号： C12Q1/26
摘要： Assay for testing a sample for the presence or absence of inhibition of the enzymatic conversion of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate into isopentenyl diphosphate and/or dimethylallyl diphosphate by the following steps: (a) reacting an aqueous mixture containing 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate, a 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphnte reductase, and a reducing agent under predetermined reaction conditions for a predetermined period of time; (b) analyzing the reaction mixture obtained in step (a) for the consumed amount of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate and/or NAD(P)H and/or for the produced amount of isopentenyl diphosphate, and/or dimethylallyl diphosphate and/or NAD(P) + ; (c) repeating step (a) in the presence of the sample to be tested; (d) repeating step (b) with the reaction mixture defined in step (c); (e) comparing the results of steps (b) and (d).

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