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1. 发明申请

WO2018087760A1 HIGHLY EFFICIENT AND TUNABLE SYSTEM FOR THE INCORPORATION OF UNNATURAL AMINO ACIDS INTO PROTEINS IN ESCHERICHIA COLI 审中-公开
标题翻译：将非天然氨基酸并入大肠杆菌蛋白质的高效可转化体系
公开(公告)号：WO2018087760A1
公开(公告)日：2018-05-17
申请号：PCT/IL2017/051221
申请日：2017-11-09
申请人： B. G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITY , REGENTS OF THE UNIVERSITY OF MINNESOTA
发明人： ALFONTA, Lital , NOIREAUX, Vincent , SCHLESINGER, Orr , CHEMLA, Yonatan , OZER, Eden
IPC分类号： C12P21/00 , C12N1/21
摘要： The present invention provides a composition with a cell having an orthogonal translation system, and an expression vector. The invention also provides a method of producing a protein carrying an unnatural amino acid, and a kit for use in producing a protein carrying the unnatural amino acid.
摘要翻译：本发明提供了具有细胞的组合物，所述细胞具有正交翻译系统和表达载体。本发明还提供了生产携带非天然氨基酸的蛋白质的方法，以及用于生产携带非天然氨基酸的蛋白质的试剂盒。

2. 发明申请

WO2022044011A1 OLIGONUCLEOTIDES AND METHODS OF USING SAME 审中-公开
公开(公告)号：WO2022044011A1
公开(公告)日：2022-03-03
申请号：PCT/IL2021/051044
申请日：2021-08-25
申请人： B. G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITY
发明人： KUSHMARO, Ariel , YANIV, Karin , OZER, Eden
IPC分类号： C12Q1/6806 , C12Q1/70
摘要： The present invention is directed to specific oligonucleotides, a kit comprising same, and a method of using same, such as for determining the presence of SARS-CoV-2 RNA in a biological or an environmental sample.

3. 发明申请

WO2015193897A1 GENETICALLY EXPANDED CELL FREE PROTEIN SYNTHESIS SYSTEMS, METHODS AND KITS 审中-公开
标题翻译：基因扩增无细胞免疫蛋白合成系统，方法和工具
公开(公告)号：WO2015193897A1
公开(公告)日：2015-12-23
申请号：PCT/IL2015/050616
申请日：2015-06-17
申请人： B. G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITY , ALFONTA, Lital , CHEMLA, Yonatan , OZER, Eden
发明人： B. G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITY , ALFONTA, Lital , CHEMLA, Yonatan , OZER, Eden
IPC分类号： C12P21/02 , C12P13/00
CPC分类号： C12P21/00 , C12N9/93 , C12P13/005 , C12P21/02 , C12Y601/01001 , C12Y601/01026
摘要： This invention relates to methods of producing a rare amino acid- or non-natural amino acid-containing protein in a cell free protein synthesis system and kits for use in and for accomplishing same. Specifically, the methods comprise the steps of expressing at least one orthogonal suppressor tRNA (o-tRNA)/aminoacyl-tRNA synthetase (aaRS) pair or derivatives thereof specific for incorporation of a rare amino acid- or non-natural amino acid in an E. coli organism; preparing a lysate of said E. coli organism expressing said orthogonal suppressor tRNA (o- tRNA)/aminoacyl-tRNA synthetase (aaRS) pair; and contacting said lysate with a template DNA containing a mutant gene in which at least one amino acid codon at a given site of the protein- encoding gene has been mutated into an amber or ochre mutation and further providing a cognate rare amino acid or non-natural amino acid and other factors necessary for protein synthesis; wherein protein synthesis occurs following said contact to produce a protein containing said at least one rare amino acid or said non-natural amino acid. Kits for use are described, as well.
摘要翻译：本发明涉及在无细胞蛋白质合成系统中生产稀有氨基酸或非天然氨基酸的蛋白质的方法以及用于和用于实现其的试剂盒。具体地说，该方法包括以下步骤：在E中特异性地掺入稀有氨基酸或非天然氨基酸的至少一种正交抑制基因tRNA（o-tRNA）/氨基酰-tRNA合成酶（aaRS）对或其衍生物大肠杆菌生物; 制备表达所述正交抑制子tRNA（o-tRNA）/氨酰-tRNA合成酶（aaRS）对的所述大肠杆菌生物的裂解物; 并使所述裂解物与含有突变基因的模板DNA接触，其中所述蛋白质编码基因的给定位点处的至少一个氨基酸密码子突变为琥珀色或赭色突变，并进一步提供相关的稀有氨基酸或非 - 天然氨基酸等蛋白质合成所必需的因素; 其中在所述接触之后发生蛋白质合成以产生含有所述至少一种稀有氨基酸或所述非天然氨基酸的蛋白质。也可以使用套件。

4. 发明申请

WO2021009760A1 TRANSGENIC BACTERIA AND METHODS OF USING SAME 审中-公开
公开(公告)号：WO2021009760A1
公开(公告)日：2021-01-21
申请号：PCT/IL2020/050796
申请日：2020-07-15
申请人： B. G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITY
发明人： ALFONTA, Lital , BIRNBAUM, Ramon , TSURIM, Ido , ABDU, Uri , OZER, Eden , PASI, Mor , EYZIK, Dror , BUIUM, Hadar , VITAL, Assaf , BANAR, Meytal , COHEN, Nofar , ZINGER, Hen , HORVITZ, Ofir , DAGMI, Yoav , RAHAMIM, Reut , FISHER, Tom , GLASSWINE, Erick , SCHAYEK, Or , ELIMELEH, Shir , KESHET, Yoav
IPC分类号： C12N1/20 , C12R1/43 , C07K14/325 , A01N63/20 , A01N63/23 , C12N15/32 , C12N1/21
摘要： The present invention is directed to a transgenic bacterium including at least one polynucleotide encoding a Bacillus thuringiensis israelensis (Bti) proteinaceous toxin. Further provided are compositions comprising the bacterium of the invention as well as methods of using same, such as for controlling a pest insect.

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