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1. 发明申请

WO2006110182A3 ORTHOGONAL TRANSLATION COMPONENTS FOR THE IN VIVO INCORPORATION OF UNNATURAL AMINO ACIDS 审中-公开
标题翻译：正交翻译组件用于体内掺入非天然氨基酸
公开(公告)号：WO2006110182A3
公开(公告)日：2006-12-21
申请号：PCT/US2005039210
申请日：2005-10-27
申请人： SCRIPPS RESEARCH INST , SCHULTZ PETER , ALFONTA LITAL , CHITTULURU JONATHAN R , DEITERS ALEXANDER , GROFF DAN , SUMMERER DANIEL , TSAO MENG-LIN , WANG JIANGYUN , WU NING , XIE JIANMING , ZENG HUAQIANG
发明人： SCHULTZ PETER , ALFONTA LITAL , CHITTULURU JONATHAN R , DEITERS ALEXANDER , GROFF DAN , SUMMERER DANIEL , TSAO MENG-LIN , WANG JIANGYUN , WU NING , XIE JIANMING , ZENG HUAQIANG
IPC分类号： C12N15/11 , C07K14/00 , C12N9/00 , C12N15/00
CPC分类号： C12N9/93 , C12N15/1058 , C12P21/00 , C12P21/02
摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E.coli,or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.
摘要翻译：本发明涉及正交配对的tRNA和氨酰基-tRNA合成酶，其可以将非天然氨基酸并入在真细菌宿主细胞例如大肠杆菌或真核宿主例如酵母细胞中产生的蛋白质中。本发明提供例如但不限于新颖的正交合成酶，鉴定和制备新型合成酶的方法，生产含有非天然氨基酸的蛋白质的方法和翻译系统。

2. 发明申请

WO2006001832A2 SELECTIVE INCORPORATION OF 5-HYROXYTRYPTOPHAN INTO PROTEINS IN MAMMALIAN CELLS 审中-公开
标题翻译： 5-羟色胺在马马里兰细胞中选择性蛋白质的选择性
公开(公告)号：WO2006001832A2
公开(公告)日：2006-01-05
申请号：PCT/US2004042535
申请日：2004-12-16
申请人： SCRIPPS RESEARCH INST , ZHANG ZHIWEN , ALFONTA LITAL , SCHULTZ PETER
发明人： ZHANG ZHIWEN , ALFONTA LITAL , SCHULTZ PETER
IPC分类号： C12P21/06 , C07K14/47 , C12N9/00
CPC分类号： C07K14/47 , C12N9/93 , C12N15/67 , C12P21/02
摘要： This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.
摘要翻译：本发明提供了使用正交氨酰基tRNA合成酶/ tRNA对将非天然氨基酸并入肽的方法和组合物。特别地，提供了正交对以在由蛋白石突变编码的位置中并入5-羟基-L-色氨酸。

3. 发明申请

WO2010089741A1 SYSTEMS AND METHODS FOR BIO-ELECTRICITY PRODUCTION 审中-公开
标题翻译：用于生物电生产的系统和方法
公开(公告)号：WO2010089741A1
公开(公告)日：2010-08-12
申请号：PCT/IL2010/000099
申请日：2010-02-04
申请人： BEN-GURION UNIVERSITY OF THE NEGEV RESEARCH AND DEVELOPMENT AUTHORITY , ALFONTA, Lital , FISHILEVICH, Simon , AMIR, Liron
发明人： ALFONTA, Lital , FISHILEVICH, Simon , AMIR, Liron
IPC分类号： H01M4/88 , H01M8/16 , H01M4/86
CPC分类号： H01M8/16 , H01M4/86 , H01M4/88 , Y02E60/527 , Y02P70/56
摘要： A system and method for bio-electricity production are provided. The system includes a microorganism fuel cell in which the anode compartment comprises a microorganism cell having displayed thereon an enzyme to oxidize the substrate and generate electrons. Microorganism cells, such as bacteria or yeast, may be transformed to display enzymes such as oxidases, alcohol dehydrigenases and glucoamylases.
摘要翻译：提供了用于生物电生产的系统和方法。该系统包括微生物燃料电池，其中阳极室包含微生物细胞，微生物细胞上显示有酶以氧化该基质并产生电子。微生物细胞如细菌或酵母可被转化以展示酶如氧化酶，醇脱氢酶和葡糖淀粉酶。

4. 发明申请

WO2006110182A2 ORTHOGONAL TRANSLATION COMPONENTS FOR THE IN VIVO INCORPORATION OF UNNATURAL AMINO ACIDS 审中-公开
标题翻译：非营利性翻译组件，用于公民参与联合氨基酸
公开(公告)号：WO2006110182A2
公开(公告)日：2006-10-19
申请号：PCT/US2005/039210
申请日：2005-10-27
申请人： THE SCRIPPS RESEARCH INSTITUTE , SCHULTZ, Peter , ALFONTA, Lital , CHITTULURU, Jonathan, R. , DEITERS, Alexander , GROFF, Dan , SUMMERER, Daniel , TSAO, Meng-lin , WANG, Jiangyun , WU, Ning , XIE, Jianming , ZENG, Huaqiang
发明人： SCHULTZ, Peter , ALFONTA, Lital , CHITTULURU, Jonathan, R. , DEITERS, Alexander , GROFF, Dan , SUMMERER, Daniel , TSAO, Meng-lin , WANG, Jiangyun , WU, Ning , XIE, Jianming , ZENG, Huaqiang
IPC分类号： C12Q1/68 , C12P21/06
CPC分类号： C12N9/93 , C12N15/1058 , C12P21/00 , C12P21/02
摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E.coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.
摘要翻译：本发明涉及tRNA和氨酰-tRNA合成酶的正交对，其可以将非天然氨基酸掺入到在细菌宿主细胞如大肠杆菌或真核宿主如酵母细胞中产生的蛋白质中。本发明提供了例如但不限于新的正交合成酶，用于鉴定和制备新合成酶的方法，用于产生含有非天然氨基酸的蛋白质的方法和翻译系统。

5. 发明申请

WO2015193897A1 GENETICALLY EXPANDED CELL FREE PROTEIN SYNTHESIS SYSTEMS, METHODS AND KITS 审中-公开
标题翻译：基因扩增无细胞免疫蛋白合成系统，方法和工具
公开(公告)号：WO2015193897A1
公开(公告)日：2015-12-23
申请号：PCT/IL2015/050616
申请日：2015-06-17
申请人： B. G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITY , ALFONTA, Lital , CHEMLA, Yonatan , OZER, Eden
发明人： B. G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITY , ALFONTA, Lital , CHEMLA, Yonatan , OZER, Eden
IPC分类号： C12P21/02 , C12P13/00
CPC分类号： C12P21/00 , C12N9/93 , C12P13/005 , C12P21/02 , C12Y601/01001 , C12Y601/01026
摘要： This invention relates to methods of producing a rare amino acid- or non-natural amino acid-containing protein in a cell free protein synthesis system and kits for use in and for accomplishing same. Specifically, the methods comprise the steps of expressing at least one orthogonal suppressor tRNA (o-tRNA)/aminoacyl-tRNA synthetase (aaRS) pair or derivatives thereof specific for incorporation of a rare amino acid- or non-natural amino acid in an E. coli organism; preparing a lysate of said E. coli organism expressing said orthogonal suppressor tRNA (o- tRNA)/aminoacyl-tRNA synthetase (aaRS) pair; and contacting said lysate with a template DNA containing a mutant gene in which at least one amino acid codon at a given site of the protein- encoding gene has been mutated into an amber or ochre mutation and further providing a cognate rare amino acid or non-natural amino acid and other factors necessary for protein synthesis; wherein protein synthesis occurs following said contact to produce a protein containing said at least one rare amino acid or said non-natural amino acid. Kits for use are described, as well.
摘要翻译：本发明涉及在无细胞蛋白质合成系统中生产稀有氨基酸或非天然氨基酸的蛋白质的方法以及用于和用于实现其的试剂盒。具体地说，该方法包括以下步骤：在E中特异性地掺入稀有氨基酸或非天然氨基酸的至少一种正交抑制基因tRNA（o-tRNA）/氨基酰-tRNA合成酶（aaRS）对或其衍生物大肠杆菌生物; 制备表达所述正交抑制子tRNA（o-tRNA）/氨酰-tRNA合成酶（aaRS）对的所述大肠杆菌生物的裂解物; 并使所述裂解物与含有突变基因的模板DNA接触，其中所述蛋白质编码基因的给定位点处的至少一个氨基酸密码子突变为琥珀色或赭色突变，并进一步提供相关的稀有氨基酸或非 - 天然氨基酸等蛋白质合成所必需的因素; 其中在所述接触之后发生蛋白质合成以产生含有所述至少一种稀有氨基酸或所述非天然氨基酸的蛋白质。也可以使用套件。

6. 发明申请

WO2009055616A3 GENETIC INCORPORATION OF 3-AMINOTYROSINE INTO REDUCTASES 审中-公开
公开(公告)号：WO2009055616A3
公开(公告)日：2009-04-30
申请号：PCT/US2008/081024
申请日：2008-10-23
申请人： THE SCRIPPS RESEARCH INSTITUTE , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , SEYEDSAYAMDOST, Mohammad, R. , XIE, Jianming , CHAN, Clement, Tsz , ALFONTA, Lital , SCHULTZ, Peter, G. , STUBBE, Joanne
发明人： SEYEDSAYAMDOST, Mohammad, R. , XIE, Jianming , CHAN, Clement, Tsz , ALFONTA, Lital , SCHULTZ, Peter, G. , STUBBE, Joanne
IPC分类号： C12N15/53 , C12N5/10 , C12Q1/26 , G01N33/573
摘要： This invention provides reductase proteins that comprise NH 2 Y unnatural amino acid residues, systems of orthogonal elements for incorporating NH 2 Y into reductases and methods of using NH 2 Y amino acid residues in reductases as molecular probes for probing reductases function, structure and activity.

7. 发明申请

WO2005038002A3 SITE-SPECIFIC INCORPORATION OF REDOX ACTIVE AMINO ACIDS INTO PROTEINS 审中-公开
标题翻译：红霉素活性氨基酸进入蛋白质的特定地点
公开(公告)号：WO2005038002A3
公开(公告)日：2006-11-23
申请号：PCT/US2004034089
申请日：2004-10-13
申请人： SCRIPPS RESEARCH INST , ALFONTA LITAL , SCHULTZ PETER G , ZHANG ZHIWEN
发明人： ALFONTA LITAL , SCHULTZ PETER G , ZHANG ZHIWEN
IPC分类号： C12N9/22 , C07H21/04 , C12N20060101 , C12N1/21 , C12N9/00 , C12N9/10 , C12N15/12 , C12N15/54 , C12N15/74 , C12P21/02 , C12P21/06
CPC分类号： C12N9/93 , C12P21/02
摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.
摘要翻译：提供了将氧化还原活性氨基酸掺入蛋白质的蛋白质生物合成机理组分的组成和方法，其中包括正交tRNA，正交氨酰-tRNA合成酶和tRNA /合成酶正交对。还提供了用于鉴定这些正交对的方法以及使用这些正交对产生具有氧化还原活性氨基酸的蛋白质的方法。

8. 发明申请

WO2010089741A9 SYSTEMS AND METHODS FOR BIO-ELECTRICITY PRODUCTION 审中-公开
公开(公告)号：WO2010089741A9
公开(公告)日：2010-08-12
申请号：PCT/IL2010/000099
申请日：2010-02-04
申请人： BEN-GURION UNIVERSITY OF THE NEGEV RESEARCH AND DEVELOPMENT AUTHORITY , ALFONTA, Lital , FISHILEVICH, Simon , AMIR, Liron
发明人： ALFONTA, Lital , FISHILEVICH, Simon , AMIR, Liron
IPC分类号： H01M4/88 , H01M8/16 , H01M4/86
摘要： A system and method for bio-electricity production are provided. The system includes a microorganism fuel cell in which the anode compartment comprises a microorganism cell having displayed thereon an enzyme to oxidize the substrate and generate electrons. Microorganism cells, such as bacteria or yeast, may be transformed to display enzymes such as oxidases, alcohol dehydrigenases and glucoamylases.

9. 发明申请

WO2009055616A2 GENETIC INCORPORATION OF 3-AMINOTYROSINE INTO REDUCTASES 审中-公开
标题翻译：将氨基苯甲酸的遗传转化为降低成分
公开(公告)号：WO2009055616A2
公开(公告)日：2009-04-30
申请号：PCT/US2008081024
申请日：2008-10-23
申请人： SCRIPPS RESEARCH INST , MASSACHUSETTS INST TECHNOLOGY , SEYEDSAYAMDOST MOHAMMAD R , XIE JIANMING , CHAN CLEMENT TSZ , ALFONTA LITAL , SCHULTZ PETER G , STUBBE JOANNE
发明人： SEYEDSAYAMDOST MOHAMMAD R , XIE JIANMING , CHAN CLEMENT TSZ , ALFONTA LITAL , SCHULTZ PETER G , STUBBE JOANNE
CPC分类号： C12N9/0093 , C12Q1/26 , G01N2333/9029
摘要： This invention provides reductase proteins that comprise NH2Y unnatural amino acid residues, systems of orthogonal elements for incorporating NH2Y into reductases and methods of using NH2Y amino acid residues in reductases as molecular probes for probing reductases function, structure and activity.
摘要翻译：本发明提供了包含NH2Y非天然氨基酸残基的还原酶蛋白，用于将NH2Y并入还原酶的正交元件的系统和在还原酶中使用NH 2 Y氨基酸残基作为探针还原酶功能，结构和活性的分子探针的方法。

10. 发明申请

WO2006001832A3 SELECTIVE INCORPORATION OF 5-HYROXYTRYPTOPHAN INTO PROTEINS IN MAMMALIAN CELLS 审中-公开
公开(公告)号：WO2006001832A3
公开(公告)日：2006-01-05
申请号：PCT/US2004/042535
申请日：2004-12-16
申请人： THE SCRIPPS RESEARCH INSTITUTE , ZHANG, Zhiwen , ALFONTA, Lital , SCHULTZ, Peter
发明人： ZHANG, Zhiwen , ALFONTA, Lital , SCHULTZ, Peter
IPC分类号： A61K38/43 , C12N9/00 , C12P21/06 , C07K1/00 , C07H21/02
摘要： This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.

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