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    • 4. 发明授权
    • Buffer for immunoassay, kit including same and immunoassay method using
said buffer
    • 用于免疫测定的缓冲液,包含相同的试剂盒和使用所述缓冲液的免疫测定法
    • US5486479A
    • 1996-01-23
    • US235876
    • 1994-05-02
    • Michio ItoSatoshi SugawaAtsushi Yanagida
    • Michio ItoSatoshi SugawaAtsushi Yanagida
    • G01N33/543G01N33/546
    • G01N33/54313G01N33/54393Y10S435/962Y10S436/822Y10S436/825Y10S436/909
    • The invention relates to immunoassay reagents that are both sensitive and specific and which require no sample pretreatment. The invention reagents are particularly useful for assaying digoxin concentrations in patient sera. More particularly, the invention relates to methods and kits comprising (A) an immunoreactant immobilized on a support; and, (B) a buffer agent comprising (i) a buffering agent, (ii) sodium chloride, (iii) choline chloride, (iv) a polysaccharide, (v) fatty-acid-free serum albumin, and (vi) a non-specific reaction suppressor of the formula: ##STR1## wherein X is --NH--(CO)--NH--, --NH--(CS)--NH--, or --N.dbd.C.dbd.N--, R.sub.1 and R.sub.2, which may be the same or different, are C.sub.1 -C.sub.5 linear or branched alkyl groups, or R.sub.1 and R.sub.2, together with nitrogen, is ##STR2## or the metho-p-toluenesulfonate salt thereof, Y, which may be the same or different, is any of H, OH and halogen,R.sub.3 is --NR.sub.1 R.sub.2, --NH.sub.2, --CHY, cyclohexyl, or H,m is an integer of from 2 to 5,p is an integer of from 0 to 5, andn is 0 or 1,and the addition salts thereof.
    • 本发明涉及既敏感又特异性且不需要样品预处理的免疫测定试剂。 本发明试剂特别可用于测定患者血清中的地高辛浓度。 更具体地说,本发明涉及包含(A)固定在载体上的免疫反应物的方法和试剂盒; 和(B)缓冲剂,其包含(i)缓冲剂,(ii)氯化钠,(iii)氯化胆碱,(iv)多糖,(v)不含脂肪酸的血清白蛋白和(vi) 其中X是-NH-(CO)-NH-,-NH-(CS)-NH-或-N = C = N-,R 1和R 2,其可以是下式的非特异性反应抑制剂: 相同或不同的是C1-C5直链或支链烷基,或者R1和R2与氮一起是或其对甲苯磺酸盐,可以相同或不同的Y是 H,OH和卤素中的任一个,R 3是-NR 1 R 2,-NH 2,-CHY,环己基或H,m是2至5的整数,p是0至5的整数,n是0或1 ,及其加成盐。