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1. 发明授权

US5744342A Protein having heat-resistant malate dehydrogenase activity 失效
标题翻译：具有耐热苹果酸脱氢酶活性的蛋白质
公开(公告)号：US5744342A
公开(公告)日：1998-04-28
申请号：US838418
申请日：1997-04-07
申请人： Atsushi Sogabe , Seiji Takeshima , Kazumi Yamamoto , Shinichi Teshima , Shigenori Emi , Yoshihisa Kawamura
发明人： Atsushi Sogabe , Seiji Takeshima , Kazumi Yamamoto , Shinichi Teshima , Shigenori Emi , Yoshihisa Kawamura
IPC分类号： C12N9/04 , C12N15/53
CPC分类号： C12N9/0006
摘要： A novel protein having a heat-resistant malate dehydrogenase activity, a DNA fragment having a gene encoding said protein, a recombinant vector having said DNA fragment, a transformant transformed with said vector, a method for producing the protein having heat-resistant malate dehydrogenase activity by the use of said transformant, a reagent for GOT determination, comprising the above-mentioned novel protein having a heat-resistant malate dehydrogenase activity and a method for determining GOT activity, which comprises the use of said reagent. According to the present invention, a protein having a heat-resistant malate dehydrogenase activity and having higher purity and superior heat stability can be obtained. In addition, a reagent for GOT determination which is superior in long-term storage can be prepared by the use of the protein having a heat-resistant malate dehydrogenase activity.
摘要翻译：具有耐热苹果酸脱氢酶活性的新型蛋白质，具有编码所述蛋白质的基因的DNA片段，具有所述DNA片段的重组载体，用该载体转化的转化体，具有耐热性苹果酸脱氢酶活性的蛋白质的制造方法通过使用所述转化体，用于GOT测定的试剂，其包含上述具有耐热苹果酸脱氢酶活性的新型蛋白质和确定GOT活性的方法，其包括使用所述试剂。根据本发明，可以得到具有耐热苹果酸脱氢酶活性，纯度高，热稳定性优异的蛋白质。此外，可以通过使用具有耐热苹果酸脱氢酶活性的蛋白质来制备长期保存优良的GOT测定用试剂。

2. 发明授权

US5686294A Protein having heat-resistant malate dehydrogenase activity 失效
标题翻译：具有耐热苹果酸脱氢酶活性的蛋白质
公开(公告)号：US5686294A
公开(公告)日：1997-11-11
申请号：US270013
申请日：1994-07-01
申请人： Atsushi Sogabe , Seiji Takeshima , Kazumi Yamamoto , Shinichi Teshima , Shigenori Emi , Yoshihisa Kawamura
发明人： Atsushi Sogabe , Seiji Takeshima , Kazumi Yamamoto , Shinichi Teshima , Shigenori Emi , Yoshihisa Kawamura
IPC分类号： C12N9/04 , C12N15/53
CPC分类号： C12N9/0006
摘要： A novel protein having a heat-resistant malate dehydrogenase activity, a DNA fragment having a gene encoding said protein, a recombinant vector having said DNA fragment, a transformant transformed with said vector, a method for producing the protein having heat-resistant malate dehydrogenase activity by the use of said transformant, a reagent for GOT determination, comprising the above-mentioned novel protein having a heat-resistant malate dehydrogenase activity and a method for determining GOT activity, which comprises the use of said reagent. According to the present invention, a protein having a heat-resistant malate dehydrogenase activity and having higher purity and superior heat stability can be obtained. In addition, a reagent for GOT determination which is superior in long-term storage can be prepared by the use of the protein having a heat-resistant malate dehydrogenase activity.
摘要翻译：具有耐热苹果酸脱氢酶活性的新型蛋白质，具有编码所述蛋白质的基因的DNA片段，具有所述DNA片段的重组载体，用该载体转化的转化体，具有耐热性苹果酸脱氢酶活性的蛋白质的制造方法通过使用所述转化体，用于GOT测定的试剂，其包含上述具有耐热苹果酸脱氢酶活性的新型蛋白质和确定GOT活性的方法，其包括使用所述试剂。根据本发明，可以得到具有耐热苹果酸脱氢酶活性，纯度高，热稳定性优异的蛋白质。此外，可以通过使用具有耐热苹果酸脱氢酶活性的蛋白质来制备长期保存优良的GOT测定用试剂。

3. 发明授权

US06884416B2 Stable PQQ-dependent glucose dehydrogenase composition 失效
标题翻译：稳定的PQQ依赖性葡萄糖脱氢酶组成
公开(公告)号：US06884416B2
公开(公告)日：2005-04-26
申请号：US09781703
申请日：2001-02-12
申请人： Shizuo Hattori , Atsushi Sogabe , Seiji Takeshima , Yoshihisa Kawamura
发明人： Shizuo Hattori , Atsushi Sogabe , Seiji Takeshima , Yoshihisa Kawamura
IPC分类号： C12N9/04 , C12N9/96 , C12R1/01 , C12N9/02 , C12N9/06 , C12N9/08
CPC分类号： C12N9/0006 , C12N9/96 , C12Y101/05002
摘要： The present invention provides a stable lyophilized PQQ-dependent glucose dehydrogenase composition comprising a PQQ-dependent glucose dehydrogenase together with (i) at least one compound selected from the group consisting of aspartic acid, glutamic acid, α-ketoglutaric acid, malic acid, α-ketogluconic acid, α-cyclodextrin and their salts and (ii) an albumin.
摘要翻译：本发明提供一种稳定的冻干的PQQ依赖性葡萄糖脱氢酶组合物，其包含PQQ依赖性葡萄糖脱氢酶以及（i）至少一种选自天冬氨酸，谷氨酸，α-酮戊二酸，苹果酸，α - 酮葡萄糖酸，α-环糊精及其盐和（ii）白蛋白。

4. 发明授权

US5795766A Protein having .alpha.-glucosidase activity, DNA having genetic information thereof, and production of .alpha.-glucosidase 失效
标题翻译：具有α-葡糖苷酶活性的蛋白质，具有遗传信息的DNA和α-葡糖苷酶的产生
公开(公告)号：US5795766A
公开(公告)日：1998-08-18
申请号：US611361
申请日：1996-03-06
申请人： Yuzuru Suzuki , Yukio Takii , Kazumi Yamamoto , Yoshiaki Nishiya , Atsushi Sogabe , Yukihiro Sogabe , Shigenori Emi
发明人： Yuzuru Suzuki , Yukio Takii , Kazumi Yamamoto , Yoshiaki Nishiya , Atsushi Sogabe , Yukihiro Sogabe , Shigenori Emi
IPC分类号： C12N9/26 , C12N15/56 , C12N9/28 , C12N1/00 , C12N5/10 , C12N15/63
CPC分类号： C12N9/2414 , C12N9/2408
摘要： A protein having an .alpha.-glucosidase activity, wherein its N-terminal amino acid sequence is the sequence depicted in the Sequence Listing of the present invention at Sequence No. 1; a DNA having the genetic information of the protein having an .alpha.-glucosidase activity; a recombinant vector containing said DNA; a transformant transformed with said vector; and production of .alpha.-glucosidase comprising culture of said transformant in a medium so as to grow .alpha.-glucosidase, and harvesting said .alpha.-glucosidase. According to the present invention, .alpha.-glucosidase without contamination of amylase can be easily produced in high yields and in large amounts by genetic engineering.
摘要翻译：具有α-葡糖苷酶活性的蛋白质，其N-末端氨基酸序列是序列号1的本发明的序列表中所示的序列; 具有具有α-葡糖苷酶活性的蛋白质的遗传信息的DNA; 含有所述DNA的重组载体; 用所述载体转化的转化体; 和α-葡萄糖苷酶的生产，包括在培养基中培养所述转化体，以便生长α-葡萄糖苷酶，并收获所述α-葡糖苷酶。根据本发明，通过遗传工程可以容易地以高产率和大量生产不污染淀粉酶的α-葡糖苷酶。

5. 发明授权

US5550046A DNA encoding .alpha.-glucosidase and method of producing same by genetic engineering 失效
标题翻译：编码α-葡萄糖苷酶的DNA及其基因工程生产方法
公开(公告)号：US5550046A
公开(公告)日：1996-08-27
申请号：US39777
申请日：1993-03-22
申请人： Yuzuru Suzuki , Yukio Takii , Kazumi Yamamoto , Yoshiaki Nishiya , Atsushi Sogabe , Yukihiro Sogabe , Shigenori Emi
发明人： Yuzuru Suzuki , Yukio Takii , Kazumi Yamamoto , Yoshiaki Nishiya , Atsushi Sogabe , Yukihiro Sogabe , Shigenori Emi
IPC分类号： C12N9/26 , C12N15/56 , C12N9/28 , C12N1/00 , C12N5/10 , C12N15/63
CPC分类号： C12N9/2414 , C12N9/2408
摘要： A protein having an .alpha.-glucosidase activity, wherein its N-terminal amino acid sequence is the sequence depicted in the Sequence Listing of the present invention at Sequence No: 1; a DNA having the genetic information of the protein having an .alpha.-glucosidase activity; a recombinant vector containing said DNA; a transformant transformed with said vector; and production of .alpha.-glucosidase comprising culture of said transformant in a medium so as to grow .alpha.-glucosidase, and harvesting said .alpha.-glucosidase. According to the present invention, .alpha.-glucosidase without contamination of amylase can be easily produced in high yields and in large amounts by genetic engineering.
摘要翻译：具有α-葡糖苷酶活性的蛋白质，其N-末端氨基酸序列是序列号1中本发明的序列表中所示的序列; 具有具有α-葡糖苷酶活性的蛋白质的遗传信息的DNA; 含有所述DNA的重组载体; 用所述载体转化的转化体; 和α-葡萄糖苷酶的生产，包括在培养基中培养所述转化体，以便生长α-葡萄糖苷酶，并收获所述α-葡糖苷酶。根据本发明，通过遗传工程可以容易地以高产率和大量生产不污染淀粉酶的α-葡糖苷酶。

6. 发明授权

US5298411A Glucose dehydrogenase from pseudomonas 失效
标题翻译：来自假单胞菌的葡萄糖脱氢酶
公开(公告)号：US5298411A
公开(公告)日：1994-03-29
申请号：US761280
申请日：1991-09-17
申请人： Atsushi Sogabe , Michiyo Minami , Yukihiro Sogabe , Shigenori Emi
发明人： Atsushi Sogabe , Michiyo Minami , Yukihiro Sogabe , Shigenori Emi
IPC分类号： C12N9/04 , C12R1/38 , C12N1/20
CPC分类号： C12N9/0006 , Y10S435/874
摘要： A substantially purified glucose dehydrogenase is disclosed. The enzyme has activity at a temperature of from about 30.degree. C. to about 65.degree. C. at a pH of from about pH 6 to about pH 10 and has an optimum activity at a temperature from about 50.degree. C. to about 60.degree. C. at a pH of from about 8.5 to about 9.0. The enzyme is further characterized by retaining at least 90% residual activity after treatment at 50.degree. C. for 15 minutes, being NAD or NADP dependent, having a molecular weight of about 101,000 daltons as determined by gel filtration using TSK gel, having an isoelectric point of about 4.5 by ampholyte isoelectric focusing, and having a specificity for at least .beta.-D-glucose and 2-deoxyglucose. The preferred source of the enzyme is Pseudomonas sp. FH1227.
摘要翻译：公开了一种基本上纯化的葡萄糖脱氢酶。该酶在约pH6至约pH10的pH为约30℃至约65℃的温度下具有活性，并且在约50℃至约60℃的温度下具有最佳活性在约8.5至约9.0的pH下。该酶的特征还在于在50℃下保持至少90％的残留活性，其为NAD或NADP依赖性，分子量为约101,000道尔顿，通过使用TSK凝胶的凝胶过滤测定，具有等电点通过两性电等离子聚焦约4.5的点，并且对至少（β）-D-葡萄糖和2-脱氧葡萄糖具有特异性。酶的优选来源是假单胞菌属（Pseudomonas sp。） FH1227。

7. 发明授权

US07476525B2 Modified pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase with superior substrate specificity and stability 失效
标题翻译：修饰的吡咯喹啉醌（PQQ）依赖性葡萄糖脱氢酶具有优异的底物特异性和稳定性
公开(公告)号：US07476525B2
公开(公告)日：2009-01-13
申请号：US10445789
申请日：2003-05-27
申请人： Seiji Takeshima , Atsushi Sogabe , Masanori Oka
发明人： Seiji Takeshima , Atsushi Sogabe , Masanori Oka
IPC分类号： C12N9/04 , C12N1/20 , C12N15/00 , C12Q1/00 , C12Q1/32 , C12P21/04 , C07H21/04 , C12Q1/68
CPC分类号： C12N9/0006 , C12Q1/006 , G01N2333/904
摘要： The present invention relates to modified pyrroloquinoline quinone dependent glucose dehydrogenase (PQQGDH) having lower activity with respect to disaccharides and/or greater stability than wild-type PQQGDH.
摘要翻译：本发明涉及相对于二糖具有较低活性和/或比野生型PQQGDH更高稳定性的改性吡咯并喹啉醌依赖性葡萄糖脱氢酶（PQQGDH）。

8. 再颁专利

USRE38687E1 Creatine amidinohydrolase, production thereof and use thereof 有权
标题翻译：肌酸脒基水解酶，其制备及其用途
公开(公告)号：USRE38687E1
公开(公告)日：2005-01-11
申请号：US09940941
申请日：2001-08-28
申请人： Atsushi Sogabe , Takashi Hattori , Yoshiaki Nishiya , Yoshihisa Kawamura
发明人： Atsushi Sogabe , Takashi Hattori , Yoshiaki Nishiya , Yoshihisa Kawamura
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N9/78 , C12N15/00 , C12N15/52 , C12Q1/34 , C12R1/05 , C12R1/19 , G01N33/52
CPC分类号： G01N33/52 , C12Q1/34 , G01N2333/978 , Y10S435/829
摘要： A creatine amidinohydrolase having the following physicochemical properties: Action: catalyzing the following reaction; creatine+H2O→sarcosine+urea Optimum temperature: about 40-50° C. Optimum pH: pH about 8.0-9.0 Heat stability: not more than about 50° C. (pH 7.5, 30 min) Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5-10.0 mM Molecule weight: about 43,000 (SDS-PAGE) Isoelectric point: about 3.5 4.5, a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor.

9. 发明授权

US6080553A Creatine amidinohydrolase, production thereof and use thereof 失效
标题翻译：肌酸脒基水解酶，其制备及其用途
公开(公告)号：US6080553A
公开(公告)日：2000-06-27
申请号：US799897
申请日：1997-02-13
申请人： Atsushi Sogabe , Takashi Hattori , Yoshiaki Nishiya , Yoshihisa Kawamura
发明人： Atsushi Sogabe , Takashi Hattori , Yoshiaki Nishiya , Yoshihisa Kawamura
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N9/78 , C12N15/00 , C12N15/52 , C12Q1/34 , C12R1/05 , C12R1/19 , G01N33/52 , C12N1/00 , C12N1/20
CPC分类号： G01N33/52 , C12Q1/34 , G01N2333/978 , Y10S435/829
摘要： A creatine amidinohydrolase having the following physicochemical properties:Action: catalyzing the following reaction;creatine+H.sub.2 O.fwdarw.sarcosine+ureaOptimum temperature: about 40-50.degree. C.Optimum pH: pH about 8.0-9.0Heat stability: not more than about 50.degree. C. (pH 7.5, 30 min)Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5-10.0 mMMolecular weight: about 43,000 (SDS-PAGE)Isoelectric point: about 3.5,a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor.
摘要翻译：具有以下物理化学性质的肌酸脒基水解酶：作用：催化以下反应; 肌酸+ H2O->肌氨酸+尿素最佳温度：约40-50℃最佳pH：pH约8.0-9.0热稳定性：不超过约50℃（pH 7.5,30分钟）肌酸中的Km值使用肌氨酸氧化酶和过氧化物酶的偶联试验：约3.5-10.0mM分子量：约43,000（SDS-PAGE）等电点：约3.5，一种生产所述酶的方法，包括产生所述酶的微生物的培养，使用所述酶测定样品中的肌酸或肌酸酐及其试剂。

10. 发明授权

US06867012B2 Determination method of biological component and reagent kit used therefor 失效
标题翻译：用于其的生物成分和试剂盒的测定方法
公开(公告)号：US06867012B2
公开(公告)日：2005-03-15
申请号：US09998130
申请日：2001-12-03
申请人： Takahide Kishimoto , Atsushi Sogabe , Shizuo Hattori , Masanori Oka , Yoshihisa Kawamura
发明人： Takahide Kishimoto , Atsushi Sogabe , Shizuo Hattori , Masanori Oka , Yoshihisa Kawamura
IPC分类号： C12N9/02 , C12Q1/26 , C12Q1/32 , C12Q1/48
CPC分类号： C12N9/0008 , C12Q1/26 , C12Q1/32 , C12Q1/48 , Y10S435/975
摘要： The present invention provides novel glutathione-dependent formaldehyde dehydrogenase that makes possible quantitative measurement of formaldehyde by cycling reaction, and a determination method of formaldehyde and biological components, such as creatinine, creatine, and homocysteine, which produces formaldehyde as a reaction intermediate. In addition, the present invention provides a reagent kit for the above-mentioned determination method. The present invention provides a novel determination method of a homocysteine using transferase utilizing homocysteine and other compound as a pair of substrates. Particularly, the present invention provides a determination method of homocysteine which includes bringing betaine-homocysteine methyltransferase and dimethylglycine oxidase into contact with a sample and measuring produced hydrogen peroxide or formaldehyde. Moreover, the present invention provides novel dimethylglycine oxidase stable to thiol compound, which is suitably used for the measurement. The present invention provides a reagent kit used for any of the above-mentioned determination methods of homocysteine.
摘要翻译：本发明提供了新的谷胱甘肽依赖性甲醛脱氢酶，其可以通过循环反应定量测定甲醛，以及产生甲醛作为反应中间体的甲醛和生物成分如肌酸酐，肌酸和高半胱氨酸的测定方法。此外，本发明提供了用于上述确定方法的试剂盒。本发明提供使用高半胱氨酸和其它化合物作为一对底物的转移酶的同型半胱氨酸的新型测定方法。特别地，本发明提供了同型半胱氨酸的测定方法，其包括使甜菜碱 - 高半胱氨酸甲基转移酶和二甲基甘氨酸氧化酶与样品接触并测量产生的过氧化氢或甲醛。此外，本发明提供了适用于测定的硫醇化合物稳定的新型二甲基甘氨酸氧化酶。本发明提供了用于任何上述同型半胱氨酸测定方法的试剂盒。

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