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1. 发明授权

US5298411A Glucose dehydrogenase from pseudomonas 失效
标题翻译：来自假单胞菌的葡萄糖脱氢酶
公开(公告)号：US5298411A
公开(公告)日：1994-03-29
申请号：US761280
申请日：1991-09-17
申请人： Atsushi Sogabe , Michiyo Minami , Yukihiro Sogabe , Shigenori Emi
发明人： Atsushi Sogabe , Michiyo Minami , Yukihiro Sogabe , Shigenori Emi
IPC分类号： C12N9/04 , C12R1/38 , C12N1/20
CPC分类号： C12N9/0006 , Y10S435/874
摘要： A substantially purified glucose dehydrogenase is disclosed. The enzyme has activity at a temperature of from about 30.degree. C. to about 65.degree. C. at a pH of from about pH 6 to about pH 10 and has an optimum activity at a temperature from about 50.degree. C. to about 60.degree. C. at a pH of from about 8.5 to about 9.0. The enzyme is further characterized by retaining at least 90% residual activity after treatment at 50.degree. C. for 15 minutes, being NAD or NADP dependent, having a molecular weight of about 101,000 daltons as determined by gel filtration using TSK gel, having an isoelectric point of about 4.5 by ampholyte isoelectric focusing, and having a specificity for at least .beta.-D-glucose and 2-deoxyglucose. The preferred source of the enzyme is Pseudomonas sp. FH1227.
摘要翻译：公开了一种基本上纯化的葡萄糖脱氢酶。该酶在约pH6至约pH10的pH为约30℃至约65℃的温度下具有活性，并且在约50℃至约60℃的温度下具有最佳活性在约8.5至约9.0的pH下。该酶的特征还在于在50℃下保持至少90％的残留活性，其为NAD或NADP依赖性，分子量为约101,000道尔顿，通过使用TSK凝胶的凝胶过滤测定，具有等电点通过两性电等离子聚焦约4.5的点，并且对至少（β）-D-葡萄糖和2-脱氧葡萄糖具有特异性。酶的优选来源是假单胞菌属（Pseudomonas sp。） FH1227。

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