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    • 5. 发明申请
    • Mutated Alkaline Cellulase
    • 突变的碱性纤维素酶
    • US20130029897A1
    • 2013-01-31
    • US13583508
    • 2011-03-11
    • Ayako ShimazuKeiji EndoMitsuyoshi Okuda
    • Ayako ShimazuKeiji EndoMitsuyoshi Okuda
    • C12N9/42C12N1/21C11D3/386C12N1/19C12N5/10C12N15/63C12N15/56
    • C11D3/38645C12N9/2437C12Y302/01004
    • The present invention provides a mutant alkaline cellulase having enhanced anti-redeposition ability. The present invention provides a mutant alkaline cellulase having an amino acid sequence in which at least amino acid residue selected from the amino acid residues at the positions corresponding to positions 58, 45, 52, 56, 60, 64, 66, 71, 103, 119, 122, 123, 124, 125, 127, 130, 140, 161, 164, 175, 176, 178, 179, 181, 193, 194, 195, 196, 197, 199, 202, 203, 217, 225, 227, 228, 251, 267, 272, 276, 277, 280, 282, 297, 310, 312, 318, 324, 345, 354, 356, 357, 360, 363, 368, 418, 419, 420, 421, 422, 453, 454, 455, 457, 458, 459, 494, 495, 496, 500, 501, 502, 503, 504, 550, 551, 552, 604, 605, 606, 607, 608, 640, 641, 642, 644, 645, 646, 683, 684, 685, 690, 691, 692, 693, 694, 739, 740, and 741 of the amino acid sequence of an alkaline cellulase set forth in SEQ ID NO: 2 or of the amino acid sequence of an alkaline cellulase having at least 70% identity with the amino acid sequence of SEQ ID NO: 2, is substituted with other amino acid residue.
    • 本发明提供具有增强的抗再沉积能力的突变型碱性纤维素酶。 本发明提供了一种具有氨基酸序列的突变型碱性纤维素酶,其中至少选自氨基酸残基的氨基酸残基位于对应于位置58,45,52,56,66,66,61,103,60,64,66,67,60,62,68,62,62,64,68,62,62,62,67,67,69,68,62,62,67 119,122,123,124,125,127,130,140,​​161,164,175,176,178,179,181,193,194,195,196,197,199,202,203,217,225, 227,228,251,267,272,276,277,280,282,277,310,312,318,324,345,354,356,357,360,363,368,418,419,420,421,418,417,318,472,427,268,267,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282 422,453,454,455,457,458,459,494,495,496,5500,501,502,503,504,550,551,552,604,605,606,607,608,640,641, 或SEQ ID NO:2所示的碱性纤维素酶的氨基酸序列的642,644,645,646,683,684,685,690,691,692,693,694,739,740和741,或 与SEQ ID NO:2的氨基酸序列具有至少70%同一性的碱性纤维素酶的氨基酸序列被其它氨基酸残基取代。
    • 6. 发明授权
    • Mutated alkaline cellulase
    • 突变碱性纤维素酶
    • US09169458B2
    • 2015-10-27
    • US13583508
    • 2011-03-11
    • Ayako ShimazuKeiji EndoMitsuyoshi Okuda
    • Ayako ShimazuKeiji EndoMitsuyoshi Okuda
    • C12N9/42C12N1/20C12N15/00C07H21/04C11D3/386
    • C11D3/38645C12N9/2437C12Y302/01004
    • The present invention provides a mutant alkaline cellulase having enhanced anti-redeposition ability. The present invention provides a mutant alkaline cellulase having an amino acid sequence in which at least amino acid residue selected from the amino acid residues at the positions corresponding to positions 58, 45, 52, 56, 60, 64, 66, 71, 103, 119, 122, 123, 124, 125, 127, 130, 140, 161, 164, 175, 176, 178, 179, 181, 193, 194, 195, 196, 197, 199, 202, 203, 217, 225, 227, 228, 251, 267, 272, 276, 277, 280, 282, 297, 310, 312, 318, 324, 345, 354, 356, 357, 360, 363, 368, 418, 419, 420, 421, 422, 453, 454, 455, 457, 458, 459, 494, 495, 496, 500, 501, 502, 503, 504, 550, 551, 552, 604, 605, 606, 607, 608, 640, 641, 642, 644, 645, 646, 683, 684, 685, 690, 691, 692, 693, 694, 739, 740, and 741 of the amino acid sequence of an alkaline cellulase set forth in SEQ ID NO: 2 or of the amino acid sequence of an alkaline cellulase having at least 70% identity with the amino acid sequence of SEQ ID NO: 2, is substituted with other amino acid residue.
    • 本发明提供具有增强的抗再沉积能力的突变型碱性纤维素酶。 本发明提供了一种具有氨基酸序列的突变型碱性纤维素酶,其中至少选自氨基酸残基的氨基酸残基位于对应于位置58,45,52,56,66,66,61,103,60,64,66,67,60,62,68,62,62,64,68,62,62,62,67,67,69,68,62,62,67 119,122,123,124,125,127,130,140,​​161,164,175,176,178,179,181,193,194,195,196,197,199,202,203,217,225, 227,228,251,267,272,276,277,280,282,277,310,312,318,324,345,354,356,357,360,363,368,418,419,420,421,418,417,318,472,427,268,267,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282 422,453,454,455,457,458,459,494,495,496,5500,501,502,503,504,550,551,552,604,605,606,607,608,640,641, 或SEQ ID NO:2所示的碱性纤维素酶的氨基酸序列的642,644,645,646,683,684,685,690,691,692,693,694,739,740和741,或 与SEQ ID NO:2的氨基酸序列具有至少70%同一性的碱性纤维素酶的氨基酸序列被其它氨基酸残基取代。