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1. 发明授权

US09169458B2 Mutated alkaline cellulase 有权
标题翻译：突变碱性纤维素酶
公开(公告)号：US09169458B2
公开(公告)日：2015-10-27
申请号：US13583508
申请日：2011-03-11
申请人： Ayako Shimazu , Keiji Endo , Mitsuyoshi Okuda
发明人： Ayako Shimazu , Keiji Endo , Mitsuyoshi Okuda
IPC分类号： C12N9/42 , C12N1/20 , C12N15/00 , C07H21/04 , C11D3/386
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention provides a mutant alkaline cellulase having enhanced anti-redeposition ability. The present invention provides a mutant alkaline cellulase having an amino acid sequence in which at least amino acid residue selected from the amino acid residues at the positions corresponding to positions 58, 45, 52, 56, 60, 64, 66, 71, 103, 119, 122, 123, 124, 125, 127, 130, 140, 161, 164, 175, 176, 178, 179, 181, 193, 194, 195, 196, 197, 199, 202, 203, 217, 225, 227, 228, 251, 267, 272, 276, 277, 280, 282, 297, 310, 312, 318, 324, 345, 354, 356, 357, 360, 363, 368, 418, 419, 420, 421, 422, 453, 454, 455, 457, 458, 459, 494, 495, 496, 500, 501, 502, 503, 504, 550, 551, 552, 604, 605, 606, 607, 608, 640, 641, 642, 644, 645, 646, 683, 684, 685, 690, 691, 692, 693, 694, 739, 740, and 741 of the amino acid sequence of an alkaline cellulase set forth in SEQ ID NO: 2 or of the amino acid sequence of an alkaline cellulase having at least 70% identity with the amino acid sequence of SEQ ID NO: 2, is substituted with other amino acid residue.
摘要翻译：本发明提供具有增强的抗再沉积能力的突变型碱性纤维素酶。本发明提供了一种具有氨基酸序列的突变型碱性纤维素酶，其中至少选自氨基酸残基的氨基酸残基位于对应于位置58,45,52,56,66,66,61,103,60,64,66,67,60,62,68,62,62,64,68,62,62,62,67,67,69,68,62,62,67 119,122,123,124,125,127,130,140,161,164,175,176,178,179,181,193,194,195,196,197,199,202,203,217,225， 227,228,251,267,272,276,277,280,282,277,310,312,318,324,345,354,356,357,360,363,368,418,419,420,421,418,417,318,472,427,268,267,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282 422,453,454,455,457,458,459,494,495,496,5500,501,502,503,504,550,551,552,604,605,606,607,608,640,641，或SEQ ID NO：2所示的碱性纤维素酶的氨基酸序列的642,644,645,646,683,684,685,690,691,692,693,694,739,740和741，或与SEQ ID NO：2的氨基酸序列具有至少70％同一性的碱性纤维素酶的氨基酸序列被其它氨基酸残基取代。

2. 发明申请

US20130029897A1 Mutated Alkaline Cellulase 有权
标题翻译：突变的碱性纤维素酶
公开(公告)号：US20130029897A1
公开(公告)日：2013-01-31
申请号：US13583508
申请日：2011-03-11
申请人： Ayako Shimazu , Keiji Endo , Mitsuyoshi Okuda
发明人： Ayako Shimazu , Keiji Endo , Mitsuyoshi Okuda
IPC分类号： C12N9/42 , C12N1/21 , C11D3/386 , C12N1/19 , C12N5/10 , C12N15/63 , C12N15/56
CPC分类号： C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要： The present invention provides a mutant alkaline cellulase having enhanced anti-redeposition ability. The present invention provides a mutant alkaline cellulase having an amino acid sequence in which at least amino acid residue selected from the amino acid residues at the positions corresponding to positions 58, 45, 52, 56, 60, 64, 66, 71, 103, 119, 122, 123, 124, 125, 127, 130, 140, 161, 164, 175, 176, 178, 179, 181, 193, 194, 195, 196, 197, 199, 202, 203, 217, 225, 227, 228, 251, 267, 272, 276, 277, 280, 282, 297, 310, 312, 318, 324, 345, 354, 356, 357, 360, 363, 368, 418, 419, 420, 421, 422, 453, 454, 455, 457, 458, 459, 494, 495, 496, 500, 501, 502, 503, 504, 550, 551, 552, 604, 605, 606, 607, 608, 640, 641, 642, 644, 645, 646, 683, 684, 685, 690, 691, 692, 693, 694, 739, 740, and 741 of the amino acid sequence of an alkaline cellulase set forth in SEQ ID NO: 2 or of the amino acid sequence of an alkaline cellulase having at least 70% identity with the amino acid sequence of SEQ ID NO: 2, is substituted with other amino acid residue.
摘要翻译：本发明提供具有增强的抗再沉积能力的突变型碱性纤维素酶。本发明提供了一种具有氨基酸序列的突变型碱性纤维素酶，其中至少选自氨基酸残基的氨基酸残基位于对应于位置58,45,52,56,66,66,61,103,60,64,66,67,60,62,68,62,62,64,68,62,62,62,67,67,69,68,62,62,67 119,122,123,124,125,127,130,140,161,164,175,176,178,179,181,193,194,195,196,197,199,202,203,217,225， 227,228,251,267,272,276,277,280,282,277,310,312,318,324,345,354,356,357,360,363,368,418,419,420,421,418,417,318,472,427,268,267,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282,276,277,280,282 422,453,454,455,457,458,459,494,495,496,5500,501,502,503,504,550,551,552,604,605,606,607,608,640,641，或SEQ ID NO：2所示的碱性纤维素酶的氨基酸序列的642,644,645,646,683,684,685,690,691,692,693,694,739,740和741，或与SEQ ID NO：2的氨基酸序列具有至少70％同一性的碱性纤维素酶的氨基酸序列被其它氨基酸残基取代。

3. 发明授权

US08778650B2 Alkaline protease variants 有权
标题翻译：碱性蛋白酶变体
公开(公告)号：US08778650B2
公开(公告)日：2014-07-15
申请号：US13266525
申请日：2010-04-26
申请人： Masatoshi Tohata , Mitsuyoshi Okuda , Tsuyoshi Sato , Keiji Endo , Katsuhisa Saeki
发明人： Masatoshi Tohata , Mitsuyoshi Okuda , Tsuyoshi Sato , Keiji Endo , Katsuhisa Saeki
IPC分类号： C12N9/54 , C11D3/386
CPC分类号： C11D3/38618 , C11D3/386 , C11D3/38681 , C12N9/54
摘要： The invention is directed to an alkaline protease that has the amino acid sequence of SEQ ID NO: 2 or of an amino acid sequence having an identity of 90% or more thereto, except that one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of SEQ ID NO: 2, or at positions corresponding thereto, are substituted with certain other amino acid residues. The invention is also directed to detergent compositions and culture medium that contain the protease.
摘要翻译：本发明涉及具有SEQ ID NO：2的氨基酸序列或90％以上的氨基酸序列的碱性蛋白酶，除了选自（a）的位置的一个或多个氨基酸残基）位置6，（b）位置15，（c）位置16，（d）位置65，（e）位置66，（f）位置82，（g）位置83，（h）位置204，（i） 319和（j）SEQ ID NO：2的位置337或与其相应的位置被某些其它氨基酸残基取代。本发明还涉及含有蛋白酶的洗涤剂组合物和培养基。

4. 发明申请

US20120058928A1 Alkaline Protease Variants 有权
标题翻译：碱性蛋白酶变体
公开(公告)号：US20120058928A1
公开(公告)日：2012-03-08
申请号：US13266525
申请日：2010-04-26
申请人： Masatoshi Tohata , Mitsuyoshi Okuda , Tsuyoshi Sato , Keiji Endo , Katsuhisa Saeki
发明人： Masatoshi Tohata , Mitsuyoshi Okuda , Tsuyoshi Sato , Keiji Endo , Katsuhisa Saeki
IPC分类号： C12N9/50 , C12N15/63 , C12P21/02 , C12N1/19 , C12N1/15 , C11D3/386 , C12N15/57 , C12N1/21
CPC分类号： C11D3/38618 , C11D3/386 , C11D3/38681 , C12N9/54
摘要： An alkaline protease variant derived from an alkaline protease consisting of an amino acid sequence represented by SEQ ID NO: 2 or consisting of an amino acid sequence having an identity of 90% or more therewith, which variant has mutations wherein one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of the amino acid sequence represented by SEQ ID NO: 2, or at positions corresponding thereto are substituted with the following amino acid residues: (a) or a position corresponding thereto: Typ, Leu, Val, Ile, Met, Tyr, Gln, Lys, Thr, Phe, Arg, Ser, Cys, Ala, or His; (b) or a position corresponding thereto: Glu, Met, Asp, Val, Gln, Arg, Cys, Trp, Ala, or Phe; (c) or a position corresponding thereto: Met, Glu, Arg, Val, Lys, Phe, Tyr, Ile, His, Asp, or Cys; (d) or a position corresponding thereto: Trp; (e) or a position corresponding thereto: His, Trp, Ser, or Leu; (f) or a position corresponding thereto: Ala, Glu, Gln, Ser, Cys, Gly, H is, Lys, Arg, Met, or Asn; (g) or a position corresponding thereto: Ala, Ser, or Cys; (h) or a position corresponding thereto: Glu, Asp, Cys, Val, Thr, Pro, His, Ile, Trp, Ser, Asn, Lys, or Arg; (i) or a position corresponding thereto: Trp, Val, Thr, Leu, Ile, Cys, Glu, Lys, Tyr, Arg, Phe, Gln, Met, Pro, Asp, Asn, H is, or Ser; and (j) or a position corresponding thereto: Arg, Gly, Ser, Lys, Gln, Thr, His, Ala, Cys, or Val.
摘要翻译：由碱性蛋白酶衍生的碱性蛋白酶变体，其由SEQ ID NO：2所示的氨基酸序列组成或由与其同一性为90％以上的氨基酸序列组成，所述变体具有突变，其中一个或多个氨基酸残基在从（a）位置6，（b）位置15，（c）位置16，（d）位置65，（e）位置66，（f）位置82，（g）位置83，（h） 204，（i）位置319和（j）由SEQ ID NO：2表示的氨基酸序列的位置337或与其相对应的位置被以下氨基酸残基取代：（a）或与其对应的位置： Typ，Leu，Val，Ile，Met，Tyr，Gln，Lys，Thr，Phe，Arg，Ser，Cys，Ala或His; （b）或相应位置：Glu，Met，Asp，Val，Gln，Arg，Cys，Trp，Ala或Phe; （c）或对应的位置：Met，Glu，Arg，Val，Lys，Phe，Tyr，Ile，His，Asp或Cys; （d）或与之对应的位置：Trp; （e）或与之对应的位置：His，Trp，Ser或Leu; （f）或对应位置：Ala，Glu，Gln，Ser，Cys，Gly，H is，Lys，Arg，Met或Asn; （g）或对应的位置：Ala，Ser或Cys; （h）或相应位置：Glu，Asp，Cys，Val，Thr，Pro，His，Ile，Trp，Ser，Asn，Lys或Arg; （i）或与其相对应的位置：Trp，Val，Thr，Leu，Ile，Cys，Glu，Lys，Tyr，Arg，Phe，Gln，Met，Pro，Asp，Asn，H is或Ser; 和（j）或对应的位置：Arg，Gly，Ser，Lys，Gln，Thr，His，Ala，Cys或Val。

5. 发明授权

US07429642B2 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US07429642B2
公开(公告)日：2008-09-30
申请号：US10820714
申请日：2004-04-09
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
IPC分类号： C07K1/00
CPC分类号： C12N9/54 , C11D3/386
摘要： An alkaline protease having an amino acid with one or more amino acid residues selected from among those located at (a) position 15, (b) position 16, (c) position 166, (d) position 167, (e) position 187, (f) position 346, and (g) position 405 of the amino acid sequence of SEQ No; 1, or at positions corresponding to these positions, are the following amino acid residues, respectively: (a): histidine, (b): threonine or glutamine, (c): glycine, (d): valine, (e): serine, (f): arginine, and (g); and a gene encoding the alkaline protease.
摘要翻译：一种具有一个或多个氨基酸残基的氨基酸的碱性蛋白酶，其选自位于（a）位置15，（b）位置16，（c）位置166，（d）位置167，（e）位置187，（f）位置346和（g）SEQ ID NO：1的氨基酸序列的位置405; 分别为：（a）：组氨酸，（b）：苏氨酸或谷氨酰胺，（c）：甘氨酸，（d）：缬氨酸，（e））：丝氨酸，（f）：精氨酸和（g）; 和编码碱性蛋白酶的基因。

6. 发明授权

US07405271B2 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US07405271B2
公开(公告)日：2008-07-29
申请号：US10820712
申请日：2004-04-09
申请人： Tsuyoshi Sato , Mitsuyoshi Okuda , Shingo Koyama , Yoshifumi Izawa , Tohru Kobayashi
发明人： Tsuyoshi Sato , Mitsuyoshi Okuda , Shingo Koyama , Yoshifumi Izawa , Tohru Kobayashi
IPC分类号： C07K1/00
CPC分类号： C11D3/386 , C12N9/54
摘要： An alkaline protease having an amino acid with one or more amino acid residues selected from among those located at (a) position 63, (b) position 89, (c) position 120, (d) positions 63 and 187, (e) position 226, (f) position 296, (g) position 304 of the amino acid sequence of SEQ ID NO: 1, or at positions corresponding to these positions, are the following amino acid residues, respectively: (a): serine, (b): histidine, (c): arginine, (d): serine,(e): tyrosine, (f): valine, and (g): serine; and a gene encoding the alkaline protease.
摘要翻译：一种具有一个或多个氨基酸残基的氨基酸的碱性蛋白酶，其选自（a）位置63，（b）位置89，（c）位置120，（d）位置63和187，（e）位置 226，（f）位置296，（g）SEQ ID NO：1的氨基酸序列的位置304或与这些位置相应的位置分别是以下氨基酸残基：（a）：丝氨酸，（b ）：组氨酸，（c）：精氨酸，（d）：丝氨酸，（e）：酪氨酸，（f）：缬氨酸和（g）：丝氨酸; 和编码碱性蛋白酶的基因。

7. 发明申请

US20050026804A1 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US20050026804A1
公开(公告)日：2005-02-03
申请号：US10820712
申请日：2004-04-09
申请人： Tsuyoshi Sato , Mitsuyoshi Okuda , Shingo Koyama , Yoshifumi Izawa , Tohru Kobayashi
发明人： Tsuyoshi Sato , Mitsuyoshi Okuda , Shingo Koyama , Yoshifumi Izawa , Tohru Kobayashi
IPC分类号： C12N15/09 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/54 , C11D3/00
CPC分类号： C11D3/386 , C12N9/54
摘要： An alkaline protease having an amino acid, wherein one or more amino acid residues selected from among those located at (a) position 63, (b) position 89, (c) position 120, (d) positions 63 and 187, (e) position 226, (f) position 296, (g) position 304 of the amino acid sequence of SEQ NO; 1, or at positions corresponding to these positions are the following amino acid residues, respectively: (a): serine, (b): histidine, (c): arginine, (d): serine, (e): tyrosine, (f): valine, and (g): serine; and a gene encoding the alkaline protease. The alkaline protease has for example, excellent detergency against complex soil and has improved thermal stability.
摘要翻译：具有氨基酸的碱性蛋白酶，其中选自位于（a）位置63，（b）位置89，（c）位置120，（d）位置63和187的那些中的一个或多个氨基酸残基，（e）位置226，（f）位置296，（g）SEQ ID NO：1的氨基酸序列的位置304; （a）：丝氨酸，（b）：组氨酸，（c）：精氨酸，（d）：丝氨酸，（e）：酪氨酸，（f ）：缬氨酸和（g）：丝氨酸; 和编码碱性蛋白酶的基因。碱性蛋白酶具有例如对复杂土壤的优异的去污力并且具有改善的热稳定性。

8. 发明授权

US07368273B2 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US07368273B2
公开(公告)日：2008-05-06
申请号：US10385662
申请日：2003-03-12
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Kazuhiro Saito , Nobuyuki Sumitomo , Yoshifumi Izawa , Katsuhisa Saeki , Tohru Kobayashi , Masafumi Nomura
IPC分类号： C12N9/48 , C11D3/386
CPC分类号： C11D3/386 , C12N9/54 , C12Y304/21062
摘要： An alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 17-0, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO: 2 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (e): histidine, aspartic acid, phenylalanine, lysine, asparagine, seine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (K): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): seine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine. A method to an alkaline protease having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore useful as an ingredient in a detergent.
摘要翻译：（a）位置65，（b）位置101，（c）位置163，（d）位置17-0，（e）位置171，（f）位置273，（g）位置320，（h）位置359或（i）SEQ ID NO：位置（a）：脯氨酸，位置（b）：天冬酰胺，位置（e）：组氨酸，天冬氨酸，苯丙氨酸，赖氨酸，天冬酰胺，（e）：丙氨酸，谷氨酸，甘氨酸和苏氨酸，位置（K）：异亮氨酸，甘氨酸和苏氨酸，位置（g）：缬氨酸和亮氨酸，（i）：丙氨酸，赖氨酸，谷氨酰胺，谷氨酸，精氨酸和组氨酸，位置（h）：ine碱，亮氨酸，缬氨酸，异亮氨酸和谷氨酰胺。一种碱性蛋白酶的方法，即使在高度浓缩的脂肪酸的存在下也具有活性，并且对于去除含有蛋白质，皮脂等的复合污渍具有优异的去污力，因此可用作洗涤剂中的成分。

9. 发明申请

US20050214922A1 Alkaline protease 有权
标题翻译：碱性蛋白酶
公开(公告)号：US20050214922A1
公开(公告)日：2005-09-29
申请号：US10820714
申请日：2004-04-09
申请人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
发明人： Mitsuyoshi Okuda , Tsuyoshi Sato , Yasushi Takimura , Nobuyuki Sumitomo , Tohru Kobayashi
IPC分类号： C12N15/09 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/54 , C12N9/64
CPC分类号： C12N9/54 , C11D3/386
摘要： An alkaline protease having an amino acid sequence, wherein one or more amino acid residues selected from those located at (a) position 15, (b) position 16, (c) position 166, (d) position 167, (e) position 187, (f) position 346, and (g) position 405 of the amino acid sequence of SEQ ID NO: 1, or at positions corresponding to these positions are the following amino acid residues, respectively: (a): histidine, (b): threonine or glutamine, (c): glycine, (d) valine, (e): serine, (f): arginine, and (g): aspartic acid; and a gene encoding the alkaline protease. The alkaline protease exhibits for example excellent detergency against complex soil, and improved protein secretion amount and specific activity, and attains enhanced productivity.
摘要翻译：一种具有氨基酸序列的碱性蛋白酶，其中选自位于（a）位置15，（b）位置16，（c）位置166，（d）位置167，（e）位置187的氨基酸残基中的一个或多个氨基酸残基，（f）位置346和（g）SEQ ID NO：1的氨基酸序列的位置405或对应于这些位置的位置分别是以下氨基酸残基：（a）：组氨酸，（b）：苏氨酸或谷氨酰胺，（c）：甘氨酸，（d）缬氨酸，（e）：丝氨酸，（f）：精氨酸，和（g）：天冬氨酸; 和编码碱性蛋白酶的基因。碱性蛋白酶表现出对复杂土壤的优异的去污力，以及提高的蛋白质分泌量和比活性，并且提高了生产率。

10. 发明授权

US06803222B2 Alkaline proteases 有权
标题翻译：碱性蛋白酶
公开(公告)号：US06803222B2
公开(公告)日：2004-10-12
申请号：US09985689
申请日：2001-11-05
申请人： Yuji Hatada , Akinori Ogawa , Yasushi Kageyama , Tsuyoshi Sato , Hiroyuki Araki , Nobuyuki Sumitomo , Mitsuyoshi Okuda , Katsuhisa Saeki
发明人： Yuji Hatada , Akinori Ogawa , Yasushi Kageyama , Tsuyoshi Sato , Hiroyuki Araki , Nobuyuki Sumitomo , Mitsuyoshi Okuda , Katsuhisa Saeki
IPC分类号： C12N948
CPC分类号： C12N9/54 , C11D3/38609 , C11D3/38618
摘要： The present invention relates to alkaline proteases having high specific activity and strong oxidant resistance. The present invention also relates to alkaline proteases having excellent detergency that is to be added to a detergent.
摘要翻译：本发明涉及具有高比活性和强氧化剂抗性的碱性蛋白酶。本发明还涉及具有优异的去污力的碱性蛋白酶，其被添加到洗涤剂中。

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