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    • 3. 发明申请
    • EFFICIENT ALGORITHM FOR PCR TESTING OF BLOOD SAMPLES
    • 用于血液样品PCR检测的有效算法
    • US20090263788A1
    • 2009-10-22
    • US12493622
    • 2009-06-29
    • Lorraine B. PeddadaCharles M. HeldebrantAndrew J. ConradPeter Schmid
    • Lorraine B. PeddadaCharles M. HeldebrantAndrew J. ConradPeter Schmid
    • C12Q1/70
    • B01L3/505B01L3/0296C12Q1/686C12Q1/701C12Q2537/165
    • Systems, processes, and devices are provided which are useful for testing blood or plasma donations to detect those specific donations which are contaminated by a virus above a predetermined level. Samples are formed into pools which are subsequently tested for virus contamination by a high-sensitivity test such as PCR. The pools are tested in accordance with an algorithm by which a sample from each donation is mapped to each element of an N-dimensional matrix or grid. Each element of the matrix is identified by a matrix identifier, Xrcs, where rcs defines the dimensional index. An aliquot is taken from each sample, and subpools are formed, each subpool comprising aliquots of samples in which one dimensional index is fixed. All of the subpools are tested in one PCR test cycle. The dimensional indicia of each positive subpool is evaluated mathematically in accordance with a reduction by the method of minors, thereby unambiguously identifying a unique element in the grid, thereby unambiguously identifying a uniquely positive blood or plasma donation.
    • 提供了用于测试血液或血浆捐赠以检测被预定水平以上的病毒污染的特定捐赠的系统,过程和装置。 将样品形成池,随后通过高灵敏度测试(例如PCR)测试病毒污染。 根据每个捐赠的样本被映射到N维矩阵或网格的每个元素的算法来测试池。 矩阵的每个元素由矩阵标识符Xrcs来标识,其中rcs定义维度索引。 从每个样品获取等分试样,并形成子池,每个子池包含其中一维索引固定的样品的等分试样。 所有的子项目在一个PCR测试周期中进行测试。 根据通过未成年人的方法的减少,数学地评估每个阳性子宫的维度标记,从而明确地识别网格中的唯一元素,从而明确地识别唯一的阳性血液或血浆捐赠。
    • 6. 发明授权
    • Efficient algorithm for PCR testing of blood samples
    • 血液样品PCR检测的有效算法
    • US08158341B2
    • 2012-04-17
    • US11374484
    • 2006-03-09
    • Lorraine B. PeddadaCharles M. HeldebrantAndrew J. ConradPeter Schmid
    • Lorraine B. PeddadaCharles M. HeldebrantAndrew J. ConradPeter Schmid
    • C12Q1/70C12Q1/68
    • B01L3/505B01L3/0296C12Q1/686C12Q1/701C12Q2537/165
    • A method is provided for identifying viral positive biological fluid donations in the fewest number of test cycles. The method comprises providing biological fluid donations and defining an n-dimensional matrix comprising a multiplicity of elements, each element being identified by a matrix notation comprising an index for each dimension of the array. Samples are taken from each fluid donation and mapped to a matrix element with each sample identified by its corresponding element's notation; n aliquots being taken from each sample and subpools formed from the aliquots with each subpool containing an aliquot from all samples mapped to elements in which one of the dimensional indices is fixed. Subpools are tested for viral indication and the dimensional index of each subpool that returns a positive viral indication is determined. The dimensional indices are combined to identify a subset of matrix elements that contain a viral positive sample.
    • 提供了用于在最少数量的测试周期中鉴定病毒阳性生物流体捐赠的方法。 该方法包括提供生物流体捐赠并且定义包括多个元素的n维矩阵,每个元素由包括阵列的每个维度的索引的矩阵符号标识。 从每个流体捐赠取样,并将其映射到矩阵元素,每个样本由其对应元素的符号识别; 从每个样品取出等分试样,并从等分试样形成的子泳池,每个子泳池含有来自所有样品的等分试样,其映射到其中一个维度指数是固定的元素。 测试子池的病毒指示,并确定返回阳性病毒指示的每个子池的维度指数。 组合尺寸指数以识别含有病毒阳性样品的矩阵元素的子集。
    • 9. 发明授权
    • Efficient algorithm for PCR testing of blood samples
    • 血液样品PCR检测的有效算法
    • US06566052B1
    • 2003-05-20
    • US09549477
    • 2000-04-14
    • Lorraine B. PeddadaCharles M. HeldebrantAndrew J. ConradPeter Schmid
    • Lorraine B. PeddadaCharles M. HeldebrantAndrew J. ConradPeter Schmid
    • C12Q170
    • B01L3/505B01L3/0296C12Q1/686C12Q1/701C12Q2537/165
    • Systems, processes, and devices are provided which are useful for testing blood or plasma donations to detect those specific donations which are contaminated by a virus above a predetermined level. An apparatus and process is described which forms individual, separately sealed and connected sample containers from a flexible hollow tubing segment connected to a fluid donation container. The tubing segment is sealed at spaced-apart intervals along its length, with tubing segment portions in the intervals between the seals defining containers, each of which holds a portion of a plasma sample. The contents of the containers are formed into pools which are subsequently tested for virus contamination by a high-sensitivity test such as PCR. The pools are tested in accordance with an algorithm by which a sample from each donation is mapped to each element of an N-dimensional matrix or grid. Each element of the matrix is identified by a matrix identifier, Xrcs, where rcs defines the dimensional index. An aliquot is taken from each sample, and subpools are formed, each subpool comprising aliquots of samples in which one dimensional index is fixed. All of the subpools are tested in one PCR test cycle. The dimensional indicia of each positive subpool is evaluated mathematically in accordance with a reduction by the method of minors, thereby unambiguously identifying a unique element in the grid, thereby unambiguously identifying a uniquely positive blood or plasma donation.
    • 提供了用于测试血液或血浆捐赠以检测被预定水平以上的病毒污染的特定捐赠的系统,过程和装置。 描述了一种从与流体供给容器连接的柔性中空管段形成单独的,分开的密封和连接的样品容器的装置和方法。 管段沿着其长度以间隔间隔密封,密封件之间的间隔中的管段部分限定容器,每个容器保持一部分等离子体样品。 将容器的内容物形成池,随后通过诸如PCR的高灵敏度试验来测试病毒污染。 根据每个捐赠的样本被映射到N维矩阵或网格的每个元素的算法来测试池。 矩阵的每个元素由矩阵标识符Xrcs来标识,其中rcs定义维度索引。 从每个样品获取等分试样,并形成子池,每个子池包含其中一维索引固定的样品的等分试样。 所有的子项目在一个PCR测试周期中进行测试。 根据通过未成年人的方法的减少,数学地评估每个阳性子宫的维度标记,从而明确地识别网格中的唯一元素,从而明确地识别唯一的阳性血液或血浆捐赠。
    • 10. 发明授权
    • Method of PCR testing of pooled blood samples
    • 汇集血液样本的PCR检测方法
    • US6063563A
    • 2000-05-16
    • US81926
    • 1998-05-20
    • Lorraine B. PeddadaCharles M. HeldebrantAndrew J. Conrad
    • Lorraine B. PeddadaCharles M. HeldebrantAndrew J. Conrad
    • C12N15/09A61K39/12A61K48/00B01L3/00C12Q1/68C12Q1/70G01N33/483G01N33/50G01N35/00G01N35/02C07H21/00
    • B01L3/505B01L3/0296C12Q1/686C12Q1/701
    • Systems, processes, and devices are provided which are useful for testing blood or plasma donations to detect those specific donations which are contaminated by a virus above a predetermined level. An apparatus and process is described which forms individual, separately sealed and connected sample containers from a flexible hollow tubing segment connected to a fluid donation container. The tubing segment is sealed at spaced-apart intervals along its length, with tubing segment portions in the intervals between the seals defining containers, each of which holds a portion of a plasma sample. The contents of the containers are formed into pools which are subsequently tested for virus contamination by a high-sensitivity test such as PCR The pools are tested in accordance with an algorithm by which a sample from each donation is mapped to each element of an N-dimensional matrix or grid. Each element of the matrix is identified by a matrix identifier, X.sub.rcs, where rcs defines the dimensional index. An aliquot is taken from each sample, and subpools are formed, each subpool comprising aliquots of samples in which one dimensional index is fixed. All of the subpools are tested in one PCR test cycle. The dimensional indicia of each positive subpool is evaluated mathematically in accordance with a reduction by the method of minors, thereby unambiguously identifying a unique element in the grid, thereby unambiguously identifying a uniquely positive blood or plasma donation.
    • 提供了用于测试血液或血浆捐赠以检测被预定水平以上的病毒污染的特定捐赠的系统,过程和装置。 描述了一种从与流体供给容器连接的柔性中空管段形成单独的,分开的密封和连接的样品容器的装置和方法。 管段沿着其长度以间隔间隔密封,密封件之间的间隔中的管段部分限定容器,每个容器保持一部分等离子体样品。 容器的内容物形成池,其随后通过诸如PCR的高灵敏度测试来检测病毒污染。根据将来自每个捐赠的样品映射到N-末端的每个元件的算法来测试池。 三维矩阵或网格。 矩阵的每个元素由矩阵标识符Xrcs来标识,其中rcs定义维度索引。 从每个样品获取等分试样,并形成子池,每个子池包含其中一维索引固定的样品的等分试样。 所有的子项目在一个PCR测试周期中进行测试。 根据通过未成年人的方法的减少,数学地评估每个阳性子宫的维度标记,从而明确地识别网格中的唯一元素,从而明确地识别唯一的阳性血液或血浆捐赠。