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1. 发明授权

US5508164A Isolation of biological materials using magnetic particles 失效
标题翻译：使用磁性颗粒分离生物材料
公开(公告)号：US5508164A
公开(公告)日：1996-04-16
申请号：US146434
申请日：1993-10-29
申请人： Albert P. Kausch , Sandya Narayanswami , Jerry E. Manning , Barbara A. Hamkalo
发明人： Albert P. Kausch , Sandya Narayanswami , Jerry E. Manning , Barbara A. Hamkalo
IPC分类号： B03C1/00 , C12M1/00 , C12N15/10 , C12Q1/68 , G01N33/483 , G01N33/53 , G01N33/543 , G01N33/547 , G01N33/553 , G01N33/58 , G01N35/00 , G01N35/10 , C12Q1/00
CPC分类号： G01N33/54353 , C12N15/1006 , C12N15/1013 , C12Q1/6834 , G01N33/54326 , G01N2035/1025 , G01N35/0098
摘要： A method for the isolation and sorting of biological materials has been developed. Biological material includes chromosomes, segments of chromosomes, cell organelles, or other minute cellular components. The biological material is separated from the cellular milieu, if necessary, and anchored to a support. Example of a support are glass coverslips, glass or polymer beads. The anchoring is by means of a reversible cross-linking system. The supported biological material is then labelled with compositions capable of binding to said material, and with magnetic particles. Examples of the binding material include nucleic acid probes and antibodies. An example of the antibodies would be those directed to histones. Other labels, for example, fluoresceinbiotin-avidin may be used. The material may be released from the support and sorted by a magnetic force. This method is an alternative to flow cytometry and presents numerous advantages in terms of time, resolution, purity, and preservation of the structure of the biological material during isolation and separation.
摘要翻译：已经开发了用于分离和分选生物材料的方法。生物材料包括染色体，染色体片段，细胞器或其他微细胞组分。如果需要，将生物材料从细胞环境中分离并锚定到载体上。支架的例子是玻璃盖玻片，玻璃或聚合物珠。锚定是借助可逆的交联系统。然后用支持的生物材料用能够结合所述材料的组合物和磁性颗粒进行标记。结合材料的实例包括核酸探针和抗体。抗体的一个例子是针对组蛋白的那些。可以使用其它标记，例如荧光素 - 生物素 - 抗生物素蛋白。材料可以从支撑件释放并且通过磁力分选。该方法是流式细胞术的替代方法，并且在分离和分离期间在生物材料的结构的时间，分辨率，纯度和保存方面呈现许多优点。

2. 发明授权

US5665582A Isolation of biological materials 失效
标题翻译：生物材料的分离
公开(公告)号：US5665582A
公开(公告)日：1997-09-09
申请号：US229288
申请日：1994-04-18
申请人： Albert P. Kausch , Sandya Narayanswami
发明人： Albert P. Kausch , Sandya Narayanswami
IPC分类号： C12N15/10 , C12Q1/68 , G01N33/543 , G01N35/00 , C12N7/02 , C08B37/04 , C12N11/06
CPC分类号： G01N33/54353 , C12N15/1006 , C12N15/1013 , C12Q1/6834 , G01N35/0098 , Y10S435/82
摘要： A method for the isolation and sorting of biological materials has been developed. Biological material includes chromosomes, segments of chromosomes, cell organelles, or other minute cellular components. The biological material is separated from the cellular milieu, if necessary, and anchored to a support. Examples of a support are glass coverslips, glass or polymer beads. The anchoring is by means of a reversible polymer and cross-linking system. The supported biological material may then be labelled with compositions capable of binding to said material, and with magnetic particles. Examples of the binding material include nucleic acid probes and antibodies. An example of the antibodies would be those directed to histones. Other labels, for example, fluorescein-biotin-avidin may be used. The material may be released from the support and sorted by a magnetic force. This method is an alternative to flow cytometry and presents numerous advantages in terms of time, resolution, purity, and preservation of the structure of the biological material during isolation and separation.
摘要翻译：已经开发了用于分离和分选生物材料的方法。生物材料包括染色体，染色体片段，细胞器或其他微细胞组分。如果需要，将生物材料从细胞环境中分离并锚定到载体上。支撑体的实例是玻璃盖玻片，玻璃或聚合物珠。锚固是借助可逆聚合物和交联体系。然后可以用能够结合所述材料的组合物和磁性颗粒来标记所支持的生物材料。结合材料的实例包括核酸探针和抗体。抗体的一个例子是针对组蛋白的那些。可以使用其它标记，例如荧光素 - 生物素 - 抗生物素蛋白。材料可以从支撑件释放并且通过磁力分选。该方法是流式细胞术的替代方法，并且在分离和分离期间在生物材料的结构的时间，分辨率，纯度和保存方面呈现许多优点。

3. 发明申请

US20100122366A1 DEVELOPMENT OF CONTROLLED TOTAL VEGETATIVE GROWTH FOR PREVENTION OF TRANSGENE ESCAPE FROM GENETICALLY MODIFIED PLANTS AND FOR ENHANCING BIOMASS PRODUCTION 审中-公开
标题翻译：控制总体植物生长发育，用于预防遗传改良植物转基因植物和增强生物量生产
公开(公告)号：US20100122366A1
公开(公告)日：2010-05-13
申请号：US12573806
申请日：2009-10-05
申请人： Hong Luo , Joel M. Chandlee , Albert P. Kausch , Melvin J. Oliver
发明人： Hong Luo , Joel M. Chandlee , Albert P. Kausch , Melvin J. Oliver
IPC分类号： A01H5/00 , C12N15/82 , A01H1/02
CPC分类号： C12N15/8265 , C12N15/827
摘要： Genes can be introduced into plants that confer desirable traits such as, drought and stress tolerance, insect and pest resistance, as well as environmental qualities such as phyto-remediation. However, possibility for transgene escape to wild and non-transformed species raises commercial and ecological concerns. Disclosed herein are methods and compositions for generating plants with total vegetative growth for the reduction, and in some examples prevention of, transgene escape. The same methods and compositions can also be used to increase biomass production in a plant.
摘要翻译：基因可以引入植物，赋予所需的性状，如干旱和胁迫耐受性，昆虫和害虫抗性，以及环境质量，如植物修复。然而，转基因逃避野生和未转化的物种的可能性引起商业和生态问题。本文公开了用于产生具有总营养生长的植物用于还原的方法和组合物，在一些实例中，预防转基因逃逸。相同的方法和组合物也可以用于增加植物中的生物量生产。

4. 发明申请

US20100031387A1 PREVENTION OF TRANSGENE ESCAPE IN GENETICALLY MODIFIED PERENNIALS 审中-公开
标题翻译：在遗传修饰过的地区预防转基因疫苗
公开(公告)号：US20100031387A1
公开(公告)日：2010-02-04
申请号：US12430766
申请日：2009-04-27
申请人： Hong Luo , Qian Hu , Kimberly Nelson Vasilchik , John P. Longo, JR. , Albert P. Kausch , Barbara Zilinskas , Subha Lakkaraju
发明人： Hong Luo , Qian Hu , Kimberly Nelson Vasilchik , John P. Longo, JR. , Albert P. Kausch , Barbara Zilinskas , Subha Lakkaraju
IPC分类号： A01H5/00 , C12N15/82 , A01H1/06
CPC分类号： C12N15/8289
摘要： Genes can be introduced into plants that confer desirable traits such as, drought and stress tolerance, insect and pest resistance, as well as environmental qualities such as phyto-remediation. However, possibility for transgene escape to wild and non-transformed species raises commercial and ecological concerns. Disclosed herein are methods and compositions for generating sterile perennial plants for the prevention of transgene escape. For example, sterile switchgrass can be produced using these methods, for example which can be used to produce biofuel.
摘要翻译：基因可以引入植物，赋予所需的性状，如干旱和胁迫耐受性，昆虫和害虫抗性，以及环境质量，如植物修复。然而，转基因逃避野生和未转化的物种的可能性引起商业和生态问题。本文公开了用于产生用于预防转基因逃逸的无菌多年生植物的方法和组合物。例如，可以使用这些方法生产无菌柳枝稷，例如可用于生产生物燃料的柳枝稷。

5. 发明授权

US5489520A Process of producing fertile transgenic zea mays plants and progeny comprising a gene encoding phosphinothricin acetyl transferase 失效
标题翻译：生产可育转基因玉米植物和后代的方法，其包含编码膦丝菌素乙酰转移酶的基因
公开(公告)号：US5489520A
公开(公告)日：1996-02-06
申请号：US233067
申请日：1994-04-26
申请人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
发明人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
IPC分类号： A01H4/00 , C07K14/415 , C12N5/00 , C12N5/04 , C12N15/05 , C12N15/82 , C12N15/87
CPC分类号： A01H4/008 , A01H4/00 , A01H4/001 , C07K14/415 , C12N15/8207 , C12N15/87 , C12N5/0025 , C12N5/04 , Y10S47/01
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译：本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

6. 发明授权

US07151204B2 Maize chloroplast aldolase promoter compositions and methods for use thereof 有权
标题翻译：玉米叶绿体醛缩酶启动子组合物及其使用方法
公开(公告)号：US07151204B2
公开(公告)日：2006-12-19
申请号：US09757089
申请日：2001-01-09
申请人： Nancy Houmard , Lucille B. Laccetti , Albert P. Kausch , Emil M. Orozco, Jr.
发明人： Nancy Houmard , Lucille B. Laccetti , Albert P. Kausch , Emil M. Orozco, Jr.
IPC分类号： C12N15/29 , C12N15/82 , C12N5/04 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8223 , C12N9/88
摘要： The current invention provides the promoter of the Zea mays nuclear gene encoding chloroplast-localized fructose-1,6-bisphosphate (F16BP) aldolase. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the chloroplastic F16BP aldolase promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.
摘要翻译：本发明提供了编码叶绿体定位的果糖-1,6-二磷酸（F16BP）醛缩酶的玉米核基因的启动子。描述了包含该序列的组合物，以及用这种组合物转化的植物。还提供了在植物中表达转基因的方法，其中包括使用这些序列。本发明的方法包括通过遗传转化直接产生具有叶绿体F16BP醛缩酶启动子的转基因植物，以及通过植物育种方法。本发明的序列代表了用于产生转基因植物的有价值的新工具，优选具有一个或多个添加的有益特征。

7. 发明授权

US5969213A Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof 失效
标题翻译：用于生产稳定转化的可育单子叶植物及其细胞的方法和组合物
公开(公告)号：US5969213A
公开(公告)日：1999-10-19
申请号：US446930
申请日：1995-05-23
申请人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , May L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
发明人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , May L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
IPC分类号： A01H4/00 , C07K14/415 , C12N5/00 , C12N5/04 , C12N15/05 , C12N15/82 , C12N15/87 , C12N15/00 , A01H1/06
CPC分类号： A01H4/008 , A01H4/00 , A01H4/001 , C07K14/415 , C12N15/8207 , C12N15/87 , C12N5/0025 , C12N5/04 , Y10S47/01
摘要： This invention relates to stable and genetically transformed cereal plants, other than maize or rice, that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said cereal plants. Cereal plants within the scope of the invention include, but are not limited to, wheat, barley and oats. The invention also pertains to transgenic cereal plants which further comprise screenable markers and transgenic seed obtained from said plants.
摘要翻译：本发明涉及除了玉米或水稻之外的稳定和遗传转化的谷类植物，其包含表达膦丝菌素乙酰转移酶的外源DNA，以在所述谷类植物中赋予对于草丁膦的抗性。本发明范围内的谷类植物包括但不限于小麦，大麦和燕麦。本发明还涉及进一步包含从所述植物获得的可筛选标记和转基因种子的转基因谷物植物。

8. 发明授权

US5780709A Transgenic maize with increased mannitol content 失效
标题翻译：具有增加的甘露醇含量的转基因玉米
公开(公告)号：US5780709A
公开(公告)日：1998-07-14
申请号：US594861
申请日：1996-01-19
申请人： Thomas R. Adams , Paul C. Anderson , Richard J. Daines , William Gordon-Kamm , Albert P. Kausch , Michael T. Mann , Peter M. Orr , David C. Warner
发明人： Thomas R. Adams , Paul C. Anderson , Richard J. Daines , William Gordon-Kamm , Albert P. Kausch , Michael T. Mann , Peter M. Orr , David C. Warner
IPC分类号： C12N9/04 , C12N9/88 , C12N15/29 , C12N15/82 , A01H5/00 , C12N15/00 , C12N15/05
CPC分类号： C12N9/0006 , C12N15/8209 , C12N15/8241 , C12N15/8245 , C12N15/8273 , C12N9/88 , Y10S47/01
摘要： The present invention provides a method for conferring tolerance or resistance to water or salt stress in a monocot plant, and/or altering the osmoprotectant content of a monocot plant, by introducing a preselected DNA segment into the plant. This invention also relates to the transformed cells and seeds, and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译：本发明提供了通过将预先选择的DNA片段引入植物中来赋予单子叶植物中耐水性或耐盐性胁迫的方法和/或改变单子叶植物的渗透保护剂含量。本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

9. 发明授权

US06803499B1 Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效
标题翻译：用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物
公开(公告)号：US06803499B1
公开(公告)日：2004-10-12
申请号：US08852340
申请日：1997-05-07
申请人： Paul C. Anderson , Christopher E. Flick , William J. Gordon-Kamm , Albert P. Kausch , Catherine J. Mackey , Emil M. Orozco , Peter Orr , Michael A. Stephens , David A. Walters , Donald S. Walters
发明人： Paul C. Anderson , Christopher E. Flick , William J. Gordon-Kamm , Albert P. Kausch , Catherine J. Mackey , Emil M. Orozco , Peter Orr , Michael A. Stephens , David A. Walters , Donald S. Walters
IPC分类号： C12N504
CPC分类号： A01H4/001 , A01H4/00 , C07K14/415 , C12N5/0025 , C12N5/04 , C12N15/8207 , C12N15/8241 , C12N15/87
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译：本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

10. 发明授权

US06281411B1 Transgenic monocots plants with increased glycine-betaine content 失效
标题翻译：具有甘氨酸 - 甜菜碱含量增加的转基因单子叶植物
公开(公告)号：US06281411B1
公开(公告)日：2001-08-28
申请号：US08599714
申请日：1996-01-19
申请人： Thomas R. Adams , Paul C. Anderson , Richard J. Daines , William Gordon-Kamm , Albert P. Kausch , Michael T. Mann , Peter M. Orr , David C. Warner
发明人： Thomas R. Adams , Paul C. Anderson , Richard J. Daines , William Gordon-Kamm , Albert P. Kausch , Michael T. Mann , Peter M. Orr , David C. Warner
IPC分类号： A01H106
CPC分类号： C12N9/88 , C12N9/0006 , C12N15/8207 , C12N15/8209 , C12N15/8241
摘要： The present invention provides a method for conferring tolerance or resistance to water or salt stress in a monocot plant, and/or altering the osmoprotectant content of a monocot plant, by introducing a preselected DNA segment into the plant. This invention also relates to the transformed cells and seeds, and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译：本发明提供了通过将预先选择的DNA片段引入植物中来赋予单子叶植物中耐水性或耐盐性胁迫的方法和/或改变单子叶植物的渗透保护剂含量。本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。

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