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1. 发明申请

US20070166726A1 Method for detecting radiation exposure 失效
标题翻译：辐射暴露检测方法
公开(公告)号：US20070166726A1
公开(公告)日：2007-07-19
申请号：US11370079
申请日：2006-03-06
申请人： Albert Fornace , Sally Amundson , Jeffrey Trent
发明人： Albert Fornace , Sally Amundson , Jeffrey Trent
IPC分类号： C12Q1/68 , C07H21/04 , C12M3/00
CPC分类号： C12Q1/689 , C12Q1/68 , C12Q1/6888 , C12Q2600/106 , C12Q2600/158 , C12Q2565/501 , C12Q2523/313
摘要： A method is disclosed for detecting exposure of organisms to biologically significant or hazardous amounts of ionizing radiation. The method uses nucleic acid microarray hybridization to evaluate biological effects, such as patterns of expression of genes after radiation exposure. Numerous genes are provided which have been found to be responsive to radiation exposure in a variety of cell lines. These genes are incorporated into probe sets, which are exposed to a labeled nucleic acid composition from a test cell, such as cDNA reverse transcribed from mRNA in the test cell, which specifically hybridizes to members of the probe set when the cell has been exposed to a biologically significant amount of ionizing radiation. Whether the nucleic acid composition hybridizes to the nucleic acid molecules representing genes that are differentially expressed is determined. The invention also includes methods for determining a dose response relationship between radiation exposure and differential expression of one or more genes, for example to determine a probable radiation dose in cells that have actually or potentially been exposed to the ionizing radiation. The invention also includes probe sets and microarrays used in this method.
摘要翻译：公开了一种用于检测生物体对生物显着或有害量的电离辐射的暴露的方法。该方法使用核酸微阵列杂交来评估生物学效应，例如辐射暴露后基因表达的模式。已经发现已经发现许多基因对多种细胞系中的辐射暴露有反应。将这些基因并入探针组中，其从测试细胞暴露于标记的核酸组合物，例如在测试细胞中从mRNA反向转录的cDNA，当细胞暴露于细胞时，该特异性与探针组的成员杂交生物显着量的电离辐射。确定核酸组合物是否与表达差异表达基因的核酸分子杂交。本发明还包括用于确定辐射暴露与一种或多种基因的差异表达之间的剂量反应关系的方法，例如确定实际上或潜在地暴露于电离辐射的细胞中的可能的辐射剂量。本发明还包括在该方法中使用的探针组和微阵列。

2. 发明申请

US20060280683A1 Method of identifying inhibitors of GADD45 polypeptide activity, and inhibitors of such activity 失效
标题翻译：识别GADD45多肽活性抑制剂的方法和这种活性的抑制剂
公开(公告)号：US20060280683A1
公开(公告)日：2006-12-14
申请号：US11506947
申请日：2006-08-17
申请人： Xin Wang , Curtis Harris , Albert Fornace , Jill Coursen , Qimin Zhan
发明人： Xin Wang , Curtis Harris , Albert Fornace , Jill Coursen , Qimin Zhan
IPC分类号： A61K49/00 , G01N33/53 , G01N33/574
CPC分类号： G01N33/5008 , C07K14/47 , G01N33/5011 , G01N33/68 , G01N33/6842 , G01N2500/00
摘要： The present invention is directed to novel methods for assaying for modulators of GADD45 polypeptide activity. The invention also provides means to sensitize a proliferating cell to a DNA base-damaging agent by administration of novel inhibitors of GADD45 polypeptide activity. The invention further provides polypeptides which interfere with the ability of Cdc2/cyclin B1 complexes to cause a pause at the G2/M stage of the cell cycle in response to GADD45, and nucleic acids which encode such polypeptides.
摘要翻译：本发明涉及用于测定GADD45多肽活性调节剂的新方法。本发明还提供了通过施用GADD45多肽活性的新型抑制剂使增殖细胞对DNA碱损伤剂敏感的方法。本发明还提供了干扰Cdc2 /细胞周期蛋白B1复合物响应于GADD45在细胞周期的G2 / M阶段停止的能力的多肽，以及编码这种多肽的核酸。

3. 发明申请

US20060088888A1 Methods of identifying inhibitors of GADD45 polypeptide activity, and inhibitors of such activity 有权
公开(公告)号：US20060088888A1
公开(公告)日：2006-04-27
申请号：US11297160
申请日：2005-12-07
申请人： Xin Wang , Curtis Harris , Albert Fornace , Jill Coursen , Qimin Zhan
发明人： Xin Wang , Curtis Harris , Albert Fornace , Jill Coursen , Qimin Zhan
IPC分类号： G01N33/53 , C07K14/705
CPC分类号： G01N33/5008 , C07K14/47 , G01N33/5011 , G01N33/68 , G01N33/6842 , G01N2500/00
摘要： The present invention is directed to novel methods for assaying for modulators of GADD45 polypeptide activity. The invention also provides means to sensitize a proliferating cell to a DNA base-damaging agent by administration of novel inhibitors of GADD45 polypeptide activity. The invention further provides polypeptides which interfere with the ability of Cdc2/cyclin B1 complexes to cause a pause at the G2/M stage of the cell cycle in response to GADD45, and nucleic acids which encode such polypeptides.

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