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1. 发明授权

US4868115A Enzyme composition 失效
标题翻译：酶组成
公开(公告)号：US4868115A
公开(公告)日：1989-09-19
申请号：US72843
申请日：1987-07-09
申请人： Akira Obayashi , Nobutsugu Hiraoka , Yukuo Ishizaki , Atsushi Ohshima , Mitsuo Kasai
发明人： Akira Obayashi , Nobutsugu Hiraoka , Yukuo Ishizaki , Atsushi Ohshima , Mitsuo Kasai
IPC分类号： G01N33/50 , C12N9/96 , C12Q1/00 , C12Q1/68
CPC分类号： C12N9/96
摘要： An enzyme composition comprising a series of enzymes used for DNA base sequence analysis by gel electrophoresis and a dihydric alcohol.
摘要翻译：一种酶组合物，其包含用于通过凝胶电泳进行DNA碱基序列分析的一系列酶和二元醇。

2. 发明授权

US4808531A New restriction enzyme and process for producing the same 失效
标题翻译：新的限制酶及其制备方法
公开(公告)号：US4808531A
公开(公告)日：1989-02-28
申请号：US756380
申请日：1985-07-18
申请人： Akira Obayashi , Nobutsugu Hiraoka , Keiko Kita , Hiroshi Nakajima
发明人： Akira Obayashi , Nobutsugu Hiraoka , Keiko Kita , Hiroshi Nakajima
IPC分类号： C12N15/09 , C12N9/16 , C12N9/22 , C12R1/01 , C12N9/14 , C12N9/00 , C12P21/00
CPC分类号： C12N9/22 , Y10S435/822
摘要： The invention provides restriction endonuclease Mf1 I capable of recognizing the base sequence as shown below on a double-stranded DNA molecule and cleaving the DNA chain at the arrow-marked positions, but has no such action when A is methylated5'--Pu.dwnarw.GATC Py--3'3'--Py CTAG.uparw.Pu--5'(wherein A represents adenosine, G guanosine, T thymidine, C cytidine, Pu adenosine or guanosine, and Py thymidine or cytidine). The restriction endonuclease is produced by culturing Microbacterium flavum IAM 1642, FERM BP-938 in a culture medium and recovering it from the culture.
摘要翻译：本发明提供了限制性内切核酸酶Mf1I，其能够识别如下所示的碱基序列在双链DNA分子上并且在箭头标记位置处切割DNA链，但当A是甲基化5'-Pu＆darr＆GATC时没有这样的作用 Py-3'3'-Py CTAG＆uarr＆Pu-5'（其中A表示腺苷，G鸟苷，T胸苷，C胞苷，Pu腺苷或鸟苷，Py胸苷或胞苷）。限制性内切酶通过在培养基中培养黄色微生物IAM1642，FERM BP-938并从培养物中回收来产生。

3. 发明授权

US4668631A Process for producing a restriction enzyme 失效
标题翻译：生产限制酶的方法
公开(公告)号：US4668631A
公开(公告)日：1987-05-26
申请号：US777674
申请日：1985-09-19
申请人： Akira Obayashi , Nobutsugu Hiraoka , Keiko Kita , Hiroshi Nakajima
发明人： Akira Obayashi , Nobutsugu Hiraoka , Keiko Kita , Hiroshi Nakajima
IPC分类号： C12N15/09 , C12N9/16 , C12N9/22 , C12R1/01
CPC分类号： C12N9/22 , Y10S435/822
摘要： A restriction endonuclease having the ability to recognize the same base sequence and cleavage sites as SacII and SstII can be produced from Gluconobacter and isolated in pure form because no other restriction enzyme is formed.
摘要翻译：具有识别与SacII和SstII相同的碱基序列和切割位点的能力的限制性内切核酸酶可由葡糖杆菌产生并以纯形式分离，因为不形成其它限制酶。

4. 发明授权

US5026645A RNA polymerase gene, microorganism having said gene and the production of RNA polymerase by the use of said microorganism 失效
标题翻译： RNA聚合酶基因，具有所述基因的微生物和通过使用所述微生物产生RNA聚合酶
公开(公告)号：US5026645A
公开(公告)日：1991-06-25
申请号：US109615
申请日：1987-10-19
申请人： Hirokazu Kotani , Nobutsugu Hiraoka , Akira Obayashi
发明人： Hirokazu Kotani , Nobutsugu Hiraoka , Akira Obayashi
IPC分类号： C12N15/09 , C12N1/20 , C12N9/12 , C12N15/00 , C12N15/54 , C12R1/19
CPC分类号： C12N9/1247
摘要： SP6 bacteriophage RNA polymerase is produced by cultivating a new microorganism (particularly new strains of Escherichia coli) harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene and recovering SP6 bacteriophage RNA polymerase from the culture broth. SP6 bacteriophage RNA polymerase gene is provided as are new microorganisms harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene.
摘要翻译：通过培养携带SP6噬菌体RNA聚合酶基因的质粒并从培养液中回收SP6噬菌体RNA聚合酶的新的微生物（特别是大肠杆菌的新菌株）产生SP6噬菌体RNA聚合酶。提供了SP6噬菌体RNA聚合酶基因，其含有携带SP6噬菌体RNA聚合酶基因的质粒的新微生物。

5. 发明授权

US4724209A Process for producing restriction enzyme 失效
标题翻译：生产限制酶的方法
公开(公告)号：US4724209A
公开(公告)日：1988-02-09
申请号：US783634
申请日：1985-10-03
申请人： Akira Obayashi , Nobutsugu Hiraoka , Keiko Kita , Hiroshi Nakajima
发明人： Akira Obayashi , Nobutsugu Hiraoka , Keiko Kita , Hiroshi Nakajima
IPC分类号： C12N15/09 , C12N9/16 , C12N9/22 , C12R1/01 , C12N15/00 , C12Q1/68
CPC分类号： C12N9/22 , Y10S435/822
摘要： A process for producing a restriction endonuclease capable of recognizing the nucleotide sequence ##STR1## (wherein A, C, G and T represent adenosine, cytidine, guanosine and thymidine, respectively) on a DNA chain and specifically cleaving the double-stranded chain at the arrow-marked positions. This process comprises growing a microorganism belonging to the genus Halococcus and capable of producing said restriction endonuclease, and collecting the enzyme thus formed from the culture broth.
摘要翻译：（其中A，C，G和T分别代表腺苷，胞苷，鸟苷和胸腺嘧啶核苷酸）分别限定在DNA链上并特异性切割双链的限制性内切核酸酶的方法链在箭头标记的位置。该方法包括生长属于卤虫属的微生物并且能够产生所述限制性内切核酸酶，并收集由培养液形成的酶。

6. 发明授权

US07942223B2 Vehicular radiator supporting apparatus 有权
标题翻译：车载散热器配套装置
公开(公告)号：US07942223B2
公开(公告)日：2011-05-17
申请号：US12427877
申请日：2009-04-22
申请人： Akira Obayashi , Akiyoshi Kato
发明人： Akira Obayashi , Akiyoshi Kato
IPC分类号： B60K11/04
CPC分类号： B60K11/04
摘要： A vehicular radiator supporting apparatus 10 has a pair of radiator support sides 16 and 18 connected to a radiator support upper 12 to be pivoted about a predetermined pivot axis O. To the pair of radiator support sides 16 and 18, a pair of abutting brackets 34 and 36 are integrally fixed at positions corresponding to a height of the bumper reinforcement 54, and they are connected to a pair of vehicle-body side brackets 38 and 40. When applied with a load exceeding a predetermined value and acting rearward of the vehicle, the pair of abutting brackets 34 and 36 are pivoted about the pivot axis O integrally with the pair of radiator support sides 16 and 18 to be displaced rearward of the vehicle. Thus, the vehicular radiator supporting apparatus 10 itself in addition to the radiator main body 20 is prevented from being damaged.
摘要翻译：车辆散热器支撑装置10具有连接到散热器支撑上部12的一对散热器支撑侧16和18，以绕预定的枢转轴线O枢转。在一对散热器支撑侧16和18中，一对邻接支架34 36对应于保险杠加强件54的高度的位置被一体地固定，并且与一对车身侧支架38,40连接。当施加超过规定值的载荷并且作用在车辆后方时，一对邻接支架34和36围绕枢转轴线O与一对散热器支撑侧16和18一体地枢转，以便在车辆后方移位。因此，除了散热器主体20之外，防止车辆用散热器支撑装置10本身被损坏。

7. 发明申请

US20080315628A1 Reinforcing member for vehicle center pillar 有权
标题翻译：车辆中心支柱加固件
公开(公告)号：US20080315628A1
公开(公告)日：2008-12-25
申请号：US12213526
申请日：2008-06-20
申请人： Akira Obayashi
发明人： Akira Obayashi
IPC分类号： B60R27/00
CPC分类号： B62D25/04 , B62D25/025
摘要： Deformation by camber recovery etc. due to non-uniform residual stress is suppressed, and occurrence of crack and burr is suppressed by improving press forming property of a roof side T-shaped portion, so that a reinforcing member having a predetermined shape accuracy is manufactured easily and inexpensively by pressing. A reinforcing member (12) is constructed by three members, i.e. an upper member (20), a lower member (22) and a central member (24). A tensile strength of the upper member (20) is selected lower than that of the central member (24), and is higher than that of the lower member (22). In comparison to a case where the upper member (20) and the central member (24) are constructed integrally by an ultra-high tensile steel plate, fluctuation of internally generated residual stress is made small. Shape accuracy is thereby improved by the reduced deforming amount due to camber recovery etc., thus press forming being facilitated. Also, a roof side T-shaped portion constructed by the upper member (20) is improved in the drawing property, and the door frame corner portions (30) and (32) are improved in the outer trimming property. Thus, the shape accuracy is improved, and yield and productivity is increased by suppression of crack generation.
摘要翻译：抑制由于不均匀残余应力引起的弯曲恢复等的变形，通过提高屋顶侧T字形部分的冲压成形性能来抑制裂纹和毛刺的发生，从而制造具有预定形状精度的加强构件轻松而便宜的按压。加强构件（12）由三个构件构成，即上构件（20），下构件（22）和中心构件（24）。上部构件（20）的拉伸强度选择为低于中心构件（24）的拉伸强度，并且高于下部构件（22）的拉伸强度。与上部构件（20）和中央构件（24）由超高强度钢板一体构成的情况相比，内部产生的残余应力的波动变小。由于由于弯曲恢复等而导致的变形量减小，因此提高了形状精度，从而便于冲压成形。此外，由上部构件（20）构成的屋顶侧T形部分的拉伸性提高，并且门框角部（30）和（32）的外部修整性得到改善。因此，提高了形状精度，并且通过抑制裂纹产生而提高了产率和生产率。

8. 发明授权

US4940837A Novel strains and their cultivation 失效
标题翻译：新品种及其种植
公开(公告)号：US4940837A
公开(公告)日：1990-07-10
申请号：US105342
申请日：1987-10-07
申请人： Yukita Kawano , Katsuhiko Kusakabe , Hitoshi Maruyama , Susumu Matsui , Tsutomu Taniguchi , Akira Obayashi
发明人： Yukita Kawano , Katsuhiko Kusakabe , Hitoshi Maruyama , Susumu Matsui , Tsutomu Taniguchi , Akira Obayashi
IPC分类号： A01G1/04 , A01H1/00 , A01H15/00 , C12N1/14 , C12R1/645
CPC分类号： A01G1/04
摘要： The invention provides novel strains of Basidiomycetes and more particularly, novel strains of Lyophyllum ulmarium. The novel strains are characterized by having a cap which is not concave when the fruiting body has matured. A process is provided comprising inoculation of Lyophyllum ulmarium with a cap which is not concave when the fruiting body has matured on a medium to form the fruiting body.Also provided is a method comprising the mating of Lyophyllum ulmarium Lu 1-8 with other Lyophyllum ulmarium and the harvesting of Lyophyllum ulmarium with a cap which is not concave when the fruiting body has matured in a term of cultivation up to harvest of the fruiting body that does not exceed 100 days.
摘要翻译：本发明提供新的担子菌菌株，更具体地说，是新菌株的叶绿素藻。新型菌株的特征在于，当子实体已经成熟时，具有不是凹的帽。提供了一种方法，其包括：当果实体在培养基上成熟以形成子实体时，用具有不凹陷的帽接种Lyophyllum ulmarium。还提供了一种方法，其包括将Lyophyllum ulmarium Lu 1-8与其他Lyophyllum ulmarium进行交配，并且当子实体在培育直至收获子实体的期限中已经成熟时收获具有不凹陷的叶绿素不超过100天。

9. 发明授权

US4454289A Polysaccharides having anticarcinogenic activity and method for producing same 失效
标题翻译：具有抗癌活性的多糖及其制备方法
公开(公告)号：US4454289A
公开(公告)日：1984-06-12
申请号：US351364
申请日：1982-02-23
申请人： Kazuo Nakajima , Yoshiaki Hirata , Hiroyuki Uchida , Tomoko Shiomi , Tsutomu Taniguchi , Akira Obayashi , Osamu Tanabe , Takuma Sasaki
发明人： Kazuo Nakajima , Yoshiaki Hirata , Hiroyuki Uchida , Tomoko Shiomi , Tsutomu Taniguchi , Akira Obayashi , Osamu Tanabe , Takuma Sasaki
IPC分类号： C12P19/00 , A61K31/715 , C07H3/06 , C12P19/04 , C08B37/00
CPC分类号： A61K31/715 , C07H3/06 , C12P19/04
摘要： The invention relates to a high molecular weight .beta.-1,3-glucan composed of repeated glucopyranose units, each unit being represented by the formula: ##STR1## The .beta.-1,3-glucan exhibits anticarcinogenic activity. A process for producing said glucan is also claimed.
摘要翻译：本发明涉及由重复的吡喃葡萄糖单元组成的高分子量β-1,3-葡聚糖，每个单元由下式表示：β-1,3-葡聚糖表现出抗癌活性。还要求生产所述葡聚糖的方法。

10. 发明授权

US08122988B2 Vehicular cooling device supporting apparatus 有权
标题翻译：车辆冷却装置支撑装置
公开(公告)号：US08122988B2
公开(公告)日：2012-02-28
申请号：US12422452
申请日：2009-04-13
申请人： Akira Obayashi , Akiyoshi Kato
发明人： Akira Obayashi , Akiyoshi Kato
IPC分类号： B60K11/04
CPC分类号： B60K11/04
摘要： A vehicular cooling device supporting apparatus has a radiator support lower 14 formed with a plurality of support-side connecting portions 32 and 34 for altering connecting positions to radiator support sides 16 and 18. This enables a layout position of a radiator main body 20 to be altered depending on size of an engine to use component parts in common. Placing the radiator main body 20 in a rearward position when the engine is small in size can suppresses damage to the radiator main body 20 upon collision of a vehicle at a low vehicle speed. In this case, it is suffice for the connecting positions of the radiator support lower 14 to the radiator support sides 16 and 18 to be altered in a vehicle fore and aft direction. Thus, the cooling device supporting apparatus can be constructed simply at the low cost with a minimum number of component parts.
摘要翻译：车辆冷却装置支撑装置具有形成有多个支撑侧连接部32和34的散热器支撑件14，用于改变与散热器支撑侧16和18的连接位置。这使得散热器主体20的布局位置能够根据发动机的尺寸而改变，以使用共同的部件。当发动机尺寸小时，将散热器主体20放置在后方位置可以抑制车辆在低车速下碰撞时对散热器主体20的损坏。在这种情况下，散热器支撑件14与散热器支撑侧16和18的连接位置在车辆前后方向上变化就足够了。因此，冷却装置支撑装置可以以低成本简单地构成最少数量的部件。

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