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    • 4. 发明授权
    • Method for detecting a bacterial pathogen
    • 检测细菌病原体的方法
    • US09181592B2
    • 2015-11-10
    • US12056633
    • 2008-03-27
    • Yiping W. HanAkihiko Ikegami
    • Yiping W. HanAkihiko Ikegami
    • C12Q1/68C12Q1/04
    • C12Q1/689C12Q1/04
    • A method is provided for detecting a bacterial pathogen in a sample. One step of the method includes obtaining a sample and then subjecting the sample to nested PCR. The nested PCR is conducted in the presence of at least two outer oligonucleotide primers complementary to a target nucleotide sequence of the bacterial pathogen so that a first amplified product is produced. The target nucleotide sequence includes at least a portion of a 16S-23S ribosomal RNA sequence. The first amplified product is subjected to the nested PCR in the presence of at least two inner oligonucleotide primers complementary to the nucleotide sequence of the first amplified product so that a second amplified product is obtained. Detection of the second amplified product indicates the presence of the bacterial pathogen in the sample.
    • 提供了用于检测样品中细菌病原体的方法。 该方法的一个步骤包括获得样品,然后使样品进行巢式PCR。 在至少两个与细菌病原体的靶核苷酸序列互补的外部寡核苷酸引物的存在下进行嵌套PCR,从而产生第一个扩增产物。 靶核苷酸序列包括16S-23S核糖体RNA序列的至少一部分。 在与第一扩增产物的核苷酸序列互补的至少两个内部寡核苷酸引物的存在下,将第一扩增产物进行嵌套PCR,从而获得第二扩增产物。 第二次扩增产物的检测表明样品中存在细菌病原体。
    • 10. 发明授权
    • Charger apparatus
    • 充电器
    • US5453819A
    • 1995-09-26
    • US214268
    • 1994-03-17
    • Hidetsugu ShimuraHajime KuriharaAkihiko IkegamiTakehiko OkamuraTakashi HamaKenjiro Yoshioka
    • Hidetsugu ShimuraHajime KuriharaAkihiko IkegamiTakehiko OkamuraTakashi HamaKenjiro Yoshioka
    • G03G15/02
    • B82Y15/00G03G15/0216
    • A charger capable of reliably and uniformly maintaining a discharging gap formed in the vicinity of a contact portion between a charging member and a photosensitive member. A charging member 101 is arranged by supporting and fixing both ends of a flexible film 102 by means of support members 103 to 105. The film 102 forms a flexible portion oriented downwardly from fixed ends S1, S2. The film 102 is brought into contact with the member 110 to be charged in a state in which an unsupported side of the film 102 is oriented toward the downstream side in the rotating direction of the member 110 to be charged. Then, the film 102 is brought into contact with the member 110 to be charged in a contact area N, and the radius of curvature of the film 102 in a zone P2 located downstream of that zone N in the rotating direction of the member 110 to be charged becomes smaller than the radius of curvature of the film 102 in an upstream zone P1.
    • 一种能够可靠且均匀地保持形成在充电构件和感光构件之间的接触部分附近的放电间隙的充电器。 通过支撑构件103至105支撑和固定柔性膜102的两端来布置充电构件101.膜102形成从固定端S1,S2向下定向的柔性部分。 膜102与构件110接触,以使膜102的未支撑侧朝向要被充电的构件110的旋转方向的下游侧的状态进行充电。 然后,使膜102与构件110接触,以在接触区域N中被填充,并且膜102在位于构件110的旋转方向的下游的区域P2中的曲率半径为 被充电变得小于上游区域P1中的膜102的曲率半径。