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1. 发明申请

US20090081656A1 METHOD FOR DETECTING A BACTERIAL PATHOGEN 有权
标题翻译：检测细菌病原体的方法
公开(公告)号：US20090081656A1
公开(公告)日：2009-03-26
申请号：US12056633
申请日：2008-03-27
申请人： Yiping W. Han , Akihiko Ikegami
发明人： Yiping W. Han , Akihiko Ikegami
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/04
摘要： A method is provided for detecting a bacterial pathogen in a sample. One step of the method includes obtaining a sample and then subjecting the sample to nested PCR. The nested PCR is conducted in the presence of at least two outer oligonucleotide primers complementary to a target nucleotide sequence of the bacterial pathogen so that a first amplified product is produced. The target nucleotide sequence includes at least a portion of a 16S-23S ribosomal RNA sequence. The first amplified product is subjected to the nested PCR in the presence of at least two inner oligonucleotide primers complementary to the nucleotide sequence of the first amplified product so that a second amplified product is obtained. Detection of the second amplified product indicates the presence of the bacterial pathogen in the sample.
摘要翻译：提供了用于检测样品中细菌病原体的方法。该方法的一个步骤包括获得样品，然后使样品进行巢式PCR。在至少两个与细菌病原体的靶核苷酸序列互补的外部寡核苷酸引物的存在下进行嵌套PCR，从而产生第一个扩增产物。靶核苷酸序列包括16S-23S核糖体RNA序列的至少一部分。在与第一扩增产物的核苷酸序列互补的至少两个内部寡核苷酸引物的存在下，将第一扩增产物进行嵌套PCR，从而获得第二扩增产物。第二次扩增产物的检测表明样品中存在细菌病原体。

2. 发明申请

US20210292400A1 METHODS FOR TREATING, PREVENTING AND DETECTING THE PROGNOSIS OF COLORECTAL CANCER 有权
公开(公告)号：US20210292400A1
公开(公告)日：2021-09-23
申请号：US17055962
申请日：2019-05-17
申请人： Yiping W. HAN
发明人： Yiping W. HAN , Piero D. DALERBA
IPC分类号： C07K16/18 , A61K45/06 , C12Q1/6886 , C12N15/113 , A61P35/00 , A61K39/395 , A61K31/7105
摘要： The present invention relates to treating cancer including colorectal cancer (CRC) by inhibiting or blocking Annexin 1. The present invention also relates to preventing CRC in a patient at high risk by inhibiting or blocking Annexin A1. The present invention also relates to reducing chemo-resistance by inhibiting or blocking Annexin A1. The present invention also relates to detecting a poor prognosis in a subject with CRC by detecting or measuring the expression level of ANXA1 and/or the level of Annexin 1 protein.

3. 发明授权

US09181592B2 Method for detecting a bacterial pathogen 有权
标题翻译：检测细菌病原体的方法
公开(公告)号：US09181592B2
公开(公告)日：2015-11-10
申请号：US12056633
申请日：2008-03-27
申请人： Yiping W. Han , Akihiko Ikegami
发明人： Yiping W. Han , Akihiko Ikegami
IPC分类号： C12Q1/68 , C12Q1/04
CPC分类号： C12Q1/689 , C12Q1/04
摘要： A method is provided for detecting a bacterial pathogen in a sample. One step of the method includes obtaining a sample and then subjecting the sample to nested PCR. The nested PCR is conducted in the presence of at least two outer oligonucleotide primers complementary to a target nucleotide sequence of the bacterial pathogen so that a first amplified product is produced. The target nucleotide sequence includes at least a portion of a 16S-23S ribosomal RNA sequence. The first amplified product is subjected to the nested PCR in the presence of at least two inner oligonucleotide primers complementary to the nucleotide sequence of the first amplified product so that a second amplified product is obtained. Detection of the second amplified product indicates the presence of the bacterial pathogen in the sample.
摘要翻译：提供了用于检测样品中细菌病原体的方法。该方法的一个步骤包括获得样品，然后使样品进行巢式PCR。在至少两个与细菌病原体的靶核苷酸序列互补的外部寡核苷酸引物的存在下进行嵌套PCR，从而产生第一个扩增产物。靶核苷酸序列包括16S-23S核糖体RNA序列的至少一部分。在与第一扩增产物的核苷酸序列互补的至少两个内部寡核苷酸引物的存在下，将第一扩增产物进行嵌套PCR，从而获得第二扩增产物。第二次扩增产物的检测表明样品中存在细菌病原体。

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