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1. 发明申请

WO2011049995A1 HOMOLOGOUS RECOMBINATION IN AN ALGAL NUCLEAR GENOME 审中-公开
标题翻译： ALGAL核基因组中的同源重组
公开(公告)号：WO2011049995A1
公开(公告)日：2011-04-28
申请号：PCT/US2010/053265
申请日：2010-10-19
申请人： AURORA BIOFUELS, INC. , KILIAN, Oliver , VICK, Bertrand
发明人： KILIAN, Oliver , VICK, Bertrand
IPC分类号： C12N15/74 , C12P19/34
CPC分类号： C12N15/79
摘要： Exemplary transformation methods are provided for introducing deoxyribonucleic acid (DNA) into the nucleus of an algal cell. A transformation construct may be prepared, with the transformation construct having a first sequence of DNA similar to a corresponding first sequence of nuclear DNA, a second sequence of DNA similar to a corresponding second sequence of the nuclear DNA, and a sequence of DNA of interest inserted between the first and second sequences of DNA of the transformation construct. A target sequence of DNA inserted between the first and second corresponding sequences of the nuclear DNA may be transformed, resulting in replacement of the target sequence of DNA with the sequence of DNA of interest. Also provided are exemplary transformation constructs, with some transformation constructs having a first sequence of DNA similar to a corresponding first sequence of nuclear DNA of an algal cell, a second sequence of DNA similar to a corresponding second sequence of nuclear DNA of the algal cell, and a sequence of DNA of interest inserted between the first and second sequences of the transformation construct.
摘要翻译：提供了将脱氧核糖核酸（DNA）引入藻细胞核的示例性转化方法。可以制备转化构建体，其中转化构建体具有与相应的核DNA序列相似的DNA的第一序列，与核DNA相应的第二序列类似的第二DNA序列，以及感兴趣的DNA序列插入转化构建体的第一和第二DNA序列之间。插入在核DNA的第一和第二相应序列之间的DNA的靶序列可能被转化，导致用目的DNA序列替换DNA的靶序列。还提供了示例性转化构建体，其中一些转化构建体具有类似于藻类细胞的核DNA的对应的第一序列的第一DNA序列，与藻类细胞的相应的第二核DNA序列相似的第二DNA序列，以及在转化构建体的第一和第二序列之间插入的目的DNA序列。

2. 发明申请

WO2009149470A1 VCP-BASED VECTORS FOR ALGAL CELL TRANSFORMATION 审中-公开
标题翻译：用于ALGAL细胞转化的基于VCP的向量
公开(公告)号：WO2009149470A1
公开(公告)日：2009-12-10
申请号：PCT/US2009/046656
申请日：2009-06-08
申请人： AURORA BIOFUELS, INC. , VICK, Bertrand , KILIAN, Oliver
发明人： VICK, Bertrand , KILIAN, Oliver
IPC分类号： C12N15/63 , C12N15/82 , C12P21/00
CPC分类号： C12N15/90 , C12N1/12 , C12N15/79 , C12N15/8206 , C12N15/8207 , C12N15/8237 , C12N15/8247
摘要： Methods and compositions are provided herein for vector-transformed algal cells The vector comprises a Violaxanthinchlorophyll a binding protein (Vcp) promoter driving expression of an antibiotic resistance gene in an algal cell, and may be used to introduce a gene (or genes) into the alga Nannochloropsis, such that the gene(s) are expressed and functional This unprecedented ability to transform Nannochloropsis with high efficiency makes possible new developments in phycology, aquaculture and biofuels applications
摘要翻译：本文提供了用于载体转化的藻类细胞的方法和组合物。载体包含驱动藻类细胞中抗生素抗性基因表达的结合蛋白（Vcp）启动子的黄花黄绿叶绿素（Vcp）启动子，并且可用于将基因（或基因）引入到藻类Nannochloropsis，使得基因被表达和功能这种以高效率转化Nannochloropsis的前所未有的能力使得可能在真菌学，水产养殖和生物燃料应用中的新发展

3. 发明申请

WO2009149465A1 TRANSFORMATION OF ALGAL CELLS 审中-公开
标题翻译： ALGAL细胞的转化
公开(公告)号：WO2009149465A1
公开(公告)日：2009-12-10
申请号：PCT/US2009/046650
申请日：2009-06-08
申请人： AURORA BIOFUELS, INC. , VICK, Bertrand , KILIAN, Oliver
发明人： VICK, Bertrand , KILIAN, Oliver
IPC分类号： C12N15/82
CPC分类号： C12N15/90 , C12N1/12 , C12N15/79 , C12N15/8206 , C12N15/8207 , C12N15/8237 , C12N15/8247
摘要： Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell.
摘要翻译：示例性方法包括通过制备转化构建体，制备用于轰击藻类细胞的颗粒，将转化构建体粘附到颗粒，用颗粒轰击藻类细胞并将藻细胞生长成菌落的方法来转化藻类细胞。转化构建体在藻细胞的核基因组内复制，并且藻细胞的生长在营养培养基中。另一个示例性方法可以包括通过在藻细胞悬浮在低电导率的溶液中的情况下向藻细胞中加入核酸来转基因修饰藻细胞的方法，通过施加电脉冲将核酸引入藻细胞在转化的藻类细胞中，并选择包含转化的藻类细胞的菌落。

4. 发明申请

WO2011011463A2 MANIPULATION OF AN ALTERNATIVE RESPIRATORY PATHWAY IN PHOTO-AUTOTROPHS 审中-公开
标题翻译：操纵光动自由空间中的替代呼吸途径
公开(公告)号：WO2011011463A2
公开(公告)日：2011-01-27
申请号：PCT/US2010/042666
申请日：2010-07-20
申请人： AURORA BIOFUELS, INC. , BAILEY, Shaun , VICK, Bertrand , MOSELEY, Jeff
发明人： BAILEY, Shaun , VICK, Bertrand , MOSELEY, Jeff
IPC分类号： C12P7/64 , C12N1/12 , C12N15/63
CPC分类号： C12N1/12 , C12P7/6463
摘要： Exemplary methods for increasing TAG production in an algal cell during imbalanced growth conditions are provided. Some methods comprise knocking out an AOX gene, wherein the AOX gene produces an amino acid sequence having substantial similarity to the amino acid sequence of SEQ. ID. NO. 2. In further methods, the algal cell may be of genus Nannochloropsis. The AOX gene may be replaced by a construct having a nucleotide sequence having substantial similarity to SEQ ID. NOS. 3 through 5 (inclusive), wherein each of the sequences are next to or in close proximity to one another in a linear fashion. In some methods, the AOX gene may be replaced via homologous recombination. As a result, lipid production by the selected recombinant algal cell may be increased over that produced by a wild-type algal cell.
摘要翻译：提供了用于在不平衡的生长条件期间增加藻细胞中TAG产生的示例性方法。一些方法包括敲除AOX基因，其中AOX基因产生与SEQ.ID.NO.1的氨基酸序列具有基本相似性的氨基酸序列。 ID。没有。 2.在其他方法中，藻细胞可以是Nannochloropsis属。 AOX基因可以被具有与SEQ ID NO.1具有基本相似性的核苷酸序列的构建体置换。 NOS。 3至5（含），其中每个序列以线性方式彼此相邻或彼此靠近。在一些方法中，AOX基因可以通过同源重组置换。结果，所选重组藻细胞的脂质产量可能会比野生型藻细胞产生的脂质产量增加。

5. 发明申请

WO2010008490A1 THE USE OF 2-HYDROXY-5-OXOPROLINE IN CONJUNCTION WITH ALGAE 审中-公开
标题翻译：使用2-羟基-5-氧杂环戊烷与阿拉伯糖连接
公开(公告)号：WO2010008490A1
公开(公告)日：2010-01-21
申请号：PCT/US2009/003819
申请日：2009-06-25
申请人： AURORA BIOFUELS, INC. , VICK, Bertrand , FLEISCHER, Daniel
发明人： VICK, Bertrand , FLEISCHER, Daniel
IPC分类号： C07D207/28
CPC分类号： C12N1/12
摘要： Provided herein are exemplary methods for the use of 2-hydroxy-5-oxoproline in conjunction with algae. One exemplary method includes applying an effective amount of 2-hydroxy-5-oxoproline to algae in an aqueous environment to accelerate creation of a high-cell density of the algae. The effective amount of the 2-hydroxy-5-oxoproline may be approximately 0.1 grams per liter of the aqueous environment, or up to approximately 0.1 grams per liter of the aqueous environment. The effective amount of the 2-hydroxy-5-oxoproline may be applied to the aqueous environment at or near a same time, or applied to the aqueous environment over a period of time. Exemplary algae cultivation systems are also provided herein. One exemplary system includes an aqueous environment having a pale-green mutant Nannochloropsis, and an effective amount of 2-hydroxy-5-oxoproline to accelerate creation of a high-cell density of the pale-green mutant Nannochloropsis.
摘要翻译：本文提供了使用2-羟基-5-氧代脯氨酸与藻类结合使用的示例性方法。一种示例性方法包括在水性环境中向藻类施加有效量的2-羟基-5-氧代脯氨酸以加速藻类的高细胞密度的产生。 2-羟基-5-氧代脯氨酸的有效量可以为约0.1克/升的水性环境，或高达约0.1克/升的水性环境。 2-羟基-5-氧代脯氨酸的有效量可以在或同时施加到水性环境中，或者在一段时间内施加到水性环境中。本文还提供了典型的藻类培养系统。一个示例性系统包括具有淡绿色突变体Nannochloropsis的水性环境和有效量的2-羟基-5-氧代脯氨酸，以加速产生浅绿色突变体Nannochloropsis的高细胞密度。

6. 发明申请

WO2010129041A1 EFFICIENT LIGHT HARVESTING 审中-公开
标题翻译：高效收藏
公开(公告)号：WO2010129041A1
公开(公告)日：2010-11-11
申请号：PCT/US2010/001315
申请日：2010-05-04
申请人： AURORA BIOFUELS, INC. , BAILEY, Shaun , TAM, Yuen, Yee , VICK, Bertrand
发明人： BAILEY, Shaun , TAM, Yuen, Yee , VICK, Bertrand
IPC分类号： C12N15/01 , C12N1/12
CPC分类号： C12Q1/02 , A01H1/04 , A01H1/06 , C12N1/12 , C12N5/04 , C12N15/01 , G01N2333/405
摘要： Various aspects provide for genetically modifying photosynthetic cells. In some cases, an integrated light harvesting efficiency of photosynthetic cells may be increased by reducing the amount of incident light that is absorbed but not used for photosynthesis. In some cases, an increased transparency may be associated with an increased light harvesting efficiency when absorption due to non-photosynthetic processes is reduced. A reduced capacity of various light-harvesting antenna apparatus may increase transparency. In some cases, a capacity of an organism to adapt to varying light levels may be reduced, and in certain cases, a modified organism may have a reduced ability to acclimate to a low light irradiance.
摘要翻译：各种方面为转基因细胞提供遗传修饰。在某些情况下，通过减少被吸收但不用于光合作用的入射光的量，可以增加光合细胞的综合光收集效率。在一些情况下，由于非光合作用过程的吸收减少，增加的透明度可能与增加的光收集效率有关。各种光采收天线装置的容量降低可能增加透明度。在某些情况下，可以减少生物体适应不同光照水平的能力，并且在某些情况下，修饰的生物可能具有适应于低光照度的降低的能力。

7. 发明申请

WO2008060571A2 METHODS AND COMPOSITIONS FOR PRODUCTION AND PURIFICATION OF BIOFUEL FROM PLANTS AND MICROALGAE 审中-公开
标题翻译：用于从植物和微藻生产和纯化生物燃料的方法和组合物
公开(公告)号：WO2008060571A2
公开(公告)日：2008-05-22
申请号：PCT/US2007/023943
申请日：2007-11-13
申请人： AURORA BIOFUELS, INC. , CASPARI, Matthew , VICK, Bertrand , RADAELLI, Guido
发明人： CASPARI, Matthew , VICK, Bertrand , RADAELLI, Guido
IPC分类号： C11B1/12
CPC分类号： C11B1/10 , C11C3/003 , Y02E50/13 , Y10S977/894
摘要： Methods and compositions are provided for producing purified oil from an organism, whether these organisms are wild type, selectively bred or genetically modified, and are suitable for the large scale production of an oil product. The organism may be an animal, a plant or a microorganism such as yeast, bacteria or algae. The organism is processed to create a biomass which can be extracted to remove the lipids contained within the biomass. The extraction produces a crude extract rich in lipids and containing residual contaminants. These contaminants are removed by contacting the crude extract with a composition that comprises a nanomaterial. Subsequently an oil product is recovered which is substantially free from residual contaminants, such as pigments.
摘要翻译：提供了用于从生物体生产纯化油的方法和组合物，无论这些生物体是野生型的，选择性繁殖的还是转基因的，并且适用于大规模生产油品。生物体可以是动物，植物或微生物如酵母，细菌或藻类。处理生物体以产生可以提取以除去生物质中所含的脂质的生物质。提取产生富含脂质并含有残留污染物的粗提取物。通过使粗提取物与包含纳米材料的组合物接触来除去这些污染物。随后，回收基本没有残留污染物如颜料的油产物。

8. 发明申请

WO2010147662A1 OPTIMIZATION OF RESPONSE TO LIGHT 审中-公开
标题翻译：对光的反应优化
公开(公告)号：WO2010147662A1
公开(公告)日：2010-12-23
申请号：PCT/US2010/001754
申请日：2010-06-16
申请人： AURORA BIOFUELS, INC. , BAILEY, Shaun , VICK, Bertrand
发明人： BAILEY, Shaun , VICK, Bertrand
IPC分类号： G01N21/00
CPC分类号： C12N13/00
摘要： Various aspects provide for exposing a substance to light. Certain aspects include exposing a suspension of photosynthetic organisms to sunlight, and may include optimizing exposure to improve photosynthesis conditions. Certain embodiments include controlling an opacity or opacity profile of a suspension of algae and/or diatoms. Optimizing exposure may include maximizing growth rate, maximizing photosynthesis efficiency, maximizing lipid production, minimizing damage, minimizing predator growth, maximizing a capacity to grow in suboptimal media ( e.g. , polluted water, brackish water, or water having a pH outside of a preferable range), minimizing requirements for nutrients, and other features.
摘要翻译：各种方面提供将物质暴露于光。某些方面包括将光合生物悬浮液暴露于阳光下，并且可能包括优化暴露以改善光合作用条件。某些实施方案包括控制藻类和/或硅藻的悬浮液的不透明度或不透明度分布。优化暴露可以包括最大化生长速率，最大化光合作用效率，最大化脂质生产，最小化损伤，最小化捕食者生长，最大限度地提高在次最佳培养基（例如污水，微咸水或pH值在优选范围之外的水）），最小化营养需求和其他功能。

9. 发明申请

WO2010011335A1 GLYPHOSATE APPLICATIONS IN AQUACULTURE 审中-公开
标题翻译：水果中的胶体应用
公开(公告)号：WO2010011335A1
公开(公告)日：2010-01-28
申请号：PCT/US2009/004296
申请日：2009-07-24
申请人： AURORA BIOFUELS, INC. , VICK, Bertrand
发明人： VICK, Bertrand
IPC分类号： A01N55/08 , A01N57/00
CPC分类号： C12N1/12 , A01N57/20 , C12N9/1092
摘要： Methods for controlling a density of algae growing in an aquatic environment are provided. Exemplary methods include applying an effective amount of glyphosate to a density of algae growing in an aquatic environment. The algae may include genus Nannochloropsis and/or Dunaliella. The algae may also include a glyphosate resistant strain of genus Nannochloropsis. The effective amount may result in an approximate concentration of between 0.1 millimolar to 0.3 millimolar glyphosate in the aquatic environment. Additionally, the aquatic environment may include seawater. The glyphosate may be applied to the aquatic environment before and/or after the aquatic environment is inoculated with algae. Alternative methods include applying an effective amount of glufosinate to a density of algae growing in an aquatic environment.
摘要翻译：提供了控制在水生环境中生长的藻类密度的方法。示例性的方法包括将有效量的草甘膦施用于在水生环境中生长的藻类的密度。藻类可以包括Nannochloropsis属和/或Dunaliella。藻类还可以包括耐南芥的草甘膦抗性菌株。有效量可导致水生环境中约0.1毫摩尔至0.3毫摩尔草甘膦的近似浓度。此外，水生环境可能包括海水。在水生环境接种藻类之前和/或之后，草甘膦可以施用于水生环境。替代方法包括将有效量的草铵膦施用于在水生环境中生长的藻类的密度。

10. 发明申请

WO2009082696A1 METHODS FOR CONCENTRATING MICROALGAE 审中-公开
标题翻译：浓缩微藻的方法
公开(公告)号：WO2009082696A1
公开(公告)日：2009-07-02
申请号：PCT/US2008/087722
申请日：2008-12-19
申请人： AURORA BIOFUELS, INC. , RADAELLI, Guido , FLEISCHER, Daniel , VICK, Bertrand , CASPARI, Matthew , WEISSMAN, Joseph , RICE, David
发明人： RADAELLI, Guido , FLEISCHER, Daniel , VICK, Bertrand , CASPARI, Matthew , WEISSMAN, Joseph , RICE, David
IPC分类号： C12N1/02 , C02F1/52
CPC分类号： C12N1/02 , C02F1/5236 , C02F1/56 , C02F11/04
摘要： The present invention provides commercially viable, large-scale methods for concentrating microalgae with an average diameter of about 20 μm or less. The methods find use in concentrating microalgae with an average diameter of about 5 μm or less, for example, Nannochloropsis .
摘要翻译：本发明提供了用于浓缩平均直径为约20μm或更小的微藻的商业可行的大规模方法。该方法可用于浓缩平均直径约5μm或更小的微藻，例如，Nannochloropsis。

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