专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2011049995A1 HOMOLOGOUS RECOMBINATION IN AN ALGAL NUCLEAR GENOME 审中-公开
标题翻译： ALGAL核基因组中的同源重组
公开(公告)号：WO2011049995A1
公开(公告)日：2011-04-28
申请号：PCT/US2010/053265
申请日：2010-10-19
申请人： AURORA BIOFUELS, INC. , KILIAN, Oliver , VICK, Bertrand
发明人： KILIAN, Oliver , VICK, Bertrand
IPC分类号： C12N15/74 , C12P19/34
CPC分类号： C12N15/79
摘要： Exemplary transformation methods are provided for introducing deoxyribonucleic acid (DNA) into the nucleus of an algal cell. A transformation construct may be prepared, with the transformation construct having a first sequence of DNA similar to a corresponding first sequence of nuclear DNA, a second sequence of DNA similar to a corresponding second sequence of the nuclear DNA, and a sequence of DNA of interest inserted between the first and second sequences of DNA of the transformation construct. A target sequence of DNA inserted between the first and second corresponding sequences of the nuclear DNA may be transformed, resulting in replacement of the target sequence of DNA with the sequence of DNA of interest. Also provided are exemplary transformation constructs, with some transformation constructs having a first sequence of DNA similar to a corresponding first sequence of nuclear DNA of an algal cell, a second sequence of DNA similar to a corresponding second sequence of nuclear DNA of the algal cell, and a sequence of DNA of interest inserted between the first and second sequences of the transformation construct.
摘要翻译：提供了将脱氧核糖核酸（DNA）引入藻细胞核的示例性转化方法。可以制备转化构建体，其中转化构建体具有与相应的核DNA序列相似的DNA的第一序列，与核DNA相应的第二序列类似的第二DNA序列，以及感兴趣的DNA序列插入转化构建体的第一和第二DNA序列之间。插入在核DNA的第一和第二相应序列之间的DNA的靶序列可能被转化，导致用目的DNA序列替换DNA的靶序列。还提供了示例性转化构建体，其中一些转化构建体具有类似于藻类细胞的核DNA的对应的第一序列的第一DNA序列，与藻类细胞的相应的第二核DNA序列相似的第二DNA序列，以及在转化构建体的第一和第二序列之间插入的目的DNA序列。

2. 发明申请

WO2010147648A1 SYSTEMS, METHODS, AND MEDIA FOR CIRCULATING FLUID IN AN ALGAE CULTIVATION POND 审中-公开
标题翻译：系统，方法和媒体，用于在ALGAE培养池中循环流体
公开(公告)号：WO2010147648A1
公开(公告)日：2010-12-23
申请号：PCT/US2010/001731
申请日：2010-06-15
申请人： AURORA BIOFUELS, INC. , PARSHEH, Mehran , SMITH, Jordan , STRUTNER, Stephen , RADAELLI, Guido
发明人： PARSHEH, Mehran , SMITH, Jordan , STRUTNER, Stephen , RADAELLI, Guido
IPC分类号： C12N1/12
CPC分类号： F04F5/54
摘要： Systems, methods and media for generating fluid flow in an algae cultivation pond are disclosed. Circulation of fluid in the algae cultivation pond is initiated via at least one jet. The circulation of fluid generates a velocity of fluid flow of at least ten centimeters per second in the algae cultivation pond. A head is provided to the at least one jet that overcomes a head loss associated with the velocity of fluid flow of at least ten centimeters per second in the algae cultivation pond.
摘要翻译：公开了在藻类养殖池中产生流体流动的系统，方法和介质。藻类养殖池中的流体循环通过至少一个射流开始。流体的循环在藻类养殖池中产生至少十厘米每秒的流体流速。头部被提供给至少一个射流，克服与藻类培养池中每秒至少十厘米的流体流速相关的头部损失。

3. 发明申请

WO2009149465A8 TRANSFORMATION OF ALGAL CELLS 审中-公开
标题翻译： ALGAL细胞的转化
公开(公告)号：WO2009149465A8
公开(公告)日：2010-07-29
申请号：PCT/US2009046650
申请日：2009-06-08
申请人： AURORA BIOFUELS INC , VICK BERTRAND , KILIAN OLIVER
发明人： VICK BERTRAND , KILIAN OLIVER
IPC分类号： C12N15/82
CPC分类号： C12N15/90 , C12N1/12 , C12N15/79 , C12N15/8206 , C12N15/8207 , C12N15/8237 , C12N15/8247
摘要： Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell.
摘要翻译：示例性方法包括通过制备转化构建体，制备用于轰击藻类细胞的颗粒，将转化构建体粘附到颗粒，用颗粒轰击藻类细胞并将藻细胞生长成菌落的方法来转化藻类细胞。转化构建体在藻细胞的核基因组内复制，并且藻细胞的生长在营养培养基中。另一个示例性方法可以包括通过在藻细胞悬浮在低电导率的溶液中的情况下向藻细胞中加入核酸来转基因修饰藻细胞的方法，通过施加电脉冲将核酸引入藻细胞在转化的藻类细胞中，并选择包含转化的藻类细胞的菌落。

4. 发明申请

WO2011011463A3 MANIPULATION OF AN ALTERNATIVE RESPIRATORY PATHWAY IN PHOTO-AUTOTROPHS 审中-公开
标题翻译：在照相机中操作替代呼吸道路
公开(公告)号：WO2011011463A3
公开(公告)日：2011-10-06
申请号：PCT/US2010042666
申请日：2010-07-20
申请人： AURORA BIOFUELS INC , BAILEY SHAUN , VICK BERTRAND , MOSELEY JEFF
发明人： BAILEY SHAUN , VICK BERTRAND , MOSELEY JEFF
IPC分类号： C12N15/09 , C07H21/00
CPC分类号： C12N1/12 , C12P7/6463
摘要： Exemplary methods for increasing TAG production in an algal cell during imbalanced growth conditions are provided. Some methods comprise knocking out an AOX gene, wherein the AOX gene produces an amino acid sequence having substantial similarity to the amino acid sequence of SEQ. ID. NO. 2. In further methods, the algal cell may be of genus Nannochloropsis. The AOX gene may be replaced by a construct having a nucleotide sequence having substantial similarity to SEQ ID. NOS. 3 through 5 (inclusive), wherein each of the sequences are next to or in close proximity to one another in a linear fashion. In some methods, the AOX gene may be replaced via homologous recombination. As a result, lipid production by the selected recombinant algal cell may be increased over that produced by a wild-type algal cell.
摘要翻译：提供了在不平衡生长条件下增加藻类细胞中TAG生成的示例性方法。一些方法包括敲除AOX基因，其中AOX基因产生与SEQ ID NO：1的氨基酸序列具有实质相似性的氨基酸序列。 ID。没有。在另外的方法中，藻类细胞可能是Nannochloropsis属。 AOX基因可以被具有与SEQ ID具有实质相似性的核苷酸序列的构建体代替。 NOS。 3至5（包括），其中每个序列以线性方式彼此相邻或彼此靠近。在一些方法中，AOX基因可以通过同源重组取代。结果，所选择的重组藻细胞的脂质生产可能比由野生型藻细胞产生的脂质产生增加。

5. 发明申请

WO2010129041A1 EFFICIENT LIGHT HARVESTING 审中-公开
标题翻译：高效收藏
公开(公告)号：WO2010129041A1
公开(公告)日：2010-11-11
申请号：PCT/US2010/001315
申请日：2010-05-04
申请人： AURORA BIOFUELS, INC. , BAILEY, Shaun , TAM, Yuen, Yee , VICK, Bertrand
发明人： BAILEY, Shaun , TAM, Yuen, Yee , VICK, Bertrand
IPC分类号： C12N15/01 , C12N1/12
CPC分类号： C12Q1/02 , A01H1/04 , A01H1/06 , C12N1/12 , C12N5/04 , C12N15/01 , G01N2333/405
摘要： Various aspects provide for genetically modifying photosynthetic cells. In some cases, an integrated light harvesting efficiency of photosynthetic cells may be increased by reducing the amount of incident light that is absorbed but not used for photosynthesis. In some cases, an increased transparency may be associated with an increased light harvesting efficiency when absorption due to non-photosynthetic processes is reduced. A reduced capacity of various light-harvesting antenna apparatus may increase transparency. In some cases, a capacity of an organism to adapt to varying light levels may be reduced, and in certain cases, a modified organism may have a reduced ability to acclimate to a low light irradiance.
摘要翻译：各种方面为转基因细胞提供遗传修饰。在某些情况下，通过减少被吸收但不用于光合作用的入射光的量，可以增加光合细胞的综合光收集效率。在一些情况下，由于非光合作用过程的吸收减少，增加的透明度可能与增加的光收集效率有关。各种光采收天线装置的容量降低可能增加透明度。在某些情况下，可以减少生物体适应不同光照水平的能力，并且在某些情况下，修饰的生物可能具有适应于低光照度的降低的能力。

6. 发明申请

WO2009149470A1 VCP-BASED VECTORS FOR ALGAL CELL TRANSFORMATION 审中-公开
标题翻译：用于ALGAL细胞转化的基于VCP的向量
公开(公告)号：WO2009149470A1
公开(公告)日：2009-12-10
申请号：PCT/US2009/046656
申请日：2009-06-08
申请人： AURORA BIOFUELS, INC. , VICK, Bertrand , KILIAN, Oliver
发明人： VICK, Bertrand , KILIAN, Oliver
IPC分类号： C12N15/63 , C12N15/82 , C12P21/00
CPC分类号： C12N15/90 , C12N1/12 , C12N15/79 , C12N15/8206 , C12N15/8207 , C12N15/8237 , C12N15/8247
摘要： Methods and compositions are provided herein for vector-transformed algal cells The vector comprises a Violaxanthinchlorophyll a binding protein (Vcp) promoter driving expression of an antibiotic resistance gene in an algal cell, and may be used to introduce a gene (or genes) into the alga Nannochloropsis, such that the gene(s) are expressed and functional This unprecedented ability to transform Nannochloropsis with high efficiency makes possible new developments in phycology, aquaculture and biofuels applications
摘要翻译：本文提供了用于载体转化的藻类细胞的方法和组合物。载体包含驱动藻类细胞中抗生素抗性基因表达的结合蛋白（Vcp）启动子的黄花黄绿叶绿素（Vcp）启动子，并且可用于将基因（或基因）引入到藻类Nannochloropsis，使得基因被表达和功能这种以高效率转化Nannochloropsis的前所未有的能力使得可能在真菌学，水产养殖和生物燃料应用中的新发展

7. 发明申请

WO2008060571A2 METHODS AND COMPOSITIONS FOR PRODUCTION AND PURIFICATION OF BIOFUEL FROM PLANTS AND MICROALGAE 审中-公开
标题翻译：用于从植物和微藻生产和纯化生物燃料的方法和组合物
公开(公告)号：WO2008060571A2
公开(公告)日：2008-05-22
申请号：PCT/US2007/023943
申请日：2007-11-13
申请人： AURORA BIOFUELS, INC. , CASPARI, Matthew , VICK, Bertrand , RADAELLI, Guido
发明人： CASPARI, Matthew , VICK, Bertrand , RADAELLI, Guido
IPC分类号： C11B1/12
CPC分类号： C11B1/10 , C11C3/003 , Y02E50/13 , Y10S977/894
摘要： Methods and compositions are provided for producing purified oil from an organism, whether these organisms are wild type, selectively bred or genetically modified, and are suitable for the large scale production of an oil product. The organism may be an animal, a plant or a microorganism such as yeast, bacteria or algae. The organism is processed to create a biomass which can be extracted to remove the lipids contained within the biomass. The extraction produces a crude extract rich in lipids and containing residual contaminants. These contaminants are removed by contacting the crude extract with a composition that comprises a nanomaterial. Subsequently an oil product is recovered which is substantially free from residual contaminants, such as pigments.
摘要翻译：提供了用于从生物体生产纯化油的方法和组合物，无论这些生物体是野生型的，选择性繁殖的还是转基因的，并且适用于大规模生产油品。生物体可以是动物，植物或微生物如酵母，细菌或藻类。处理生物体以产生可以提取以除去生物质中所含的脂质的生物质。提取产生富含脂质并含有残留污染物的粗提取物。通过使粗提取物与包含纳米材料的组合物接触来除去这些污染物。随后，回收基本没有残留污染物如颜料的油产物。

8. 发明申请

WO2011011463A2 MANIPULATION OF AN ALTERNATIVE RESPIRATORY PATHWAY IN PHOTO-AUTOTROPHS 审中-公开
标题翻译：操纵光动自由空间中的替代呼吸途径
公开(公告)号：WO2011011463A2
公开(公告)日：2011-01-27
申请号：PCT/US2010/042666
申请日：2010-07-20
申请人： AURORA BIOFUELS, INC. , BAILEY, Shaun , VICK, Bertrand , MOSELEY, Jeff
发明人： BAILEY, Shaun , VICK, Bertrand , MOSELEY, Jeff
IPC分类号： C12P7/64 , C12N1/12 , C12N15/63
CPC分类号： C12N1/12 , C12P7/6463
摘要： Exemplary methods for increasing TAG production in an algal cell during imbalanced growth conditions are provided. Some methods comprise knocking out an AOX gene, wherein the AOX gene produces an amino acid sequence having substantial similarity to the amino acid sequence of SEQ. ID. NO. 2. In further methods, the algal cell may be of genus Nannochloropsis. The AOX gene may be replaced by a construct having a nucleotide sequence having substantial similarity to SEQ ID. NOS. 3 through 5 (inclusive), wherein each of the sequences are next to or in close proximity to one another in a linear fashion. In some methods, the AOX gene may be replaced via homologous recombination. As a result, lipid production by the selected recombinant algal cell may be increased over that produced by a wild-type algal cell.
摘要翻译：提供了用于在不平衡的生长条件期间增加藻细胞中TAG产生的示例性方法。一些方法包括敲除AOX基因，其中AOX基因产生与SEQ.ID.NO.1的氨基酸序列具有基本相似性的氨基酸序列。 ID。没有。 2.在其他方法中，藻细胞可以是Nannochloropsis属。 AOX基因可以被具有与SEQ ID NO.1具有基本相似性的核苷酸序列的构建体置换。 NOS。 3至5（含），其中每个序列以线性方式彼此相邻或彼此靠近。在一些方法中，AOX基因可以通过同源重组置换。结果，所选重组藻细胞的脂质产量可能会比野生型藻细胞产生的脂质产量增加。

9. 发明申请

WO2011002487A1 SYSTEMS, METHODS AND MEDIA FOR CIRCULATING AND CARBONATING FLUID IN AN ALGAE CULTIVATION POND 审中-公开
标题翻译：用于循环和碳酸氢化酶的制备方法和介质
公开(公告)号：WO2011002487A1
公开(公告)日：2011-01-06
申请号：PCT/US2010/001755
申请日：2010-06-16
申请人： AURORA BIOFUELS, INC. , PARSHEH, Mehran , SMITH, Jordan , STRUTNER, Stephen , RADAELLI, Guido
发明人： PARSHEH, Mehran , SMITH, Jordan , STRUTNER, Stephen , RADAELLI, Guido
IPC分类号： C12M1/00
CPC分类号： A01G33/00 , Y02A40/88
摘要： Systems, methods and media for carbonation of fluid in an algae cultivation pond via the use of jets are disclosed. Carbon dioxide is provided to a pressurized fluid. A jet of carbonated fluid is generated from the pressurized fluid and the carbon dioxide. Circulation of the fluid in the algae cultivation pond is initiated via the jet of carbonated fluid.
摘要翻译：公开了通过使用喷射器在藻类养殖池中碳酸化的系统，方法和介质。向加压流体提供二氧化碳。从加压流体和二氧化碳产生碳酸流体射流。藻类培养池中的流体循环是通过碳酸流体喷射引发的。

10. 发明申请

WO2010090760A1 SYSTEMS AND METHODS FOR MAINTAINING THE DOMINANCE AND INCREASING THE BIOMASS PRODUCTION OF NANNOCHLOROPSIS IN AN ALGAE CULTIVATION SYSTEM 审中-公开
标题翻译：维持生态系统和增强生物量的系统和方法在阿尔法培养系统中生产NANNOCHLOROPSIS
公开(公告)号：WO2010090760A1
公开(公告)日：2010-08-12
申请号：PCT/US2010/000346
申请日：2010-02-04
申请人： AURORA BIOFUELS, INC. , WEISSMAN, Joseph , RADAELLI, Guido , RICE, David
发明人： WEISSMAN, Joseph , RADAELLI, Guido , RICE, David
IPC分类号： C12M1/00 , C12N1/12 , C12M3/00
CPC分类号： C12N1/12 , Y10S261/42
摘要： Systems and methods for maintaining the dominance and increasing the biomass production of Nannochloropsis in an algae cultivation system are provided. Exemplary methods include applying an effective amount of ozone to Nannochloropsis growing in an algae cultivation system. A further method may include applying a shock amount of ozone above 10 milligrams/liter or higher in the inlet stream flowing into the algae cultivation system. Various exemplary embodiments may include a system for maintaining dominance and increasing the biomass production of Nannochloropsis in an algae cultivation system. The system may comprise a processor, and a computer readable storage medium having instructions for execution by the processor. The instructions for execution by the processor cause the processor to maintain dominance and increase biomass production of the Nannochloropsis in the algae cultivation system.
摘要翻译：提供了保持藻类栽培系统中Nannochloropsis的优势和增加生物量生产的系统和方法。示例性的方法包括将有效量的臭氧应用于在藻类栽培系统中生长的Nannochloropsis。另外的方法可以包括在流入藻类培养系统的入口流中施加超过10毫克/升或更高的冲击量的臭氧。各种示例性实施方案可以包括用于在藻类培养系统中保持优势并增加南非氯菌的生物量产生的系统。该系统可以包括处理器和具有用于由处理器执行的指令的计算机可读存储介质。由处理器执行的指令使处理器保持藻类栽培系统中Nannochloropsis的优势并增加生物量的生产。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式