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1. 发明申请

WO2011053867A1 SYSTEMS AND METHODS FOR EXTRACTING LIPIDS FROM AND DEHYDRATING WET ALGAL BIOMASS 审中-公开
标题翻译：提取脂肪和去除水分生物量的系统和方法
公开(公告)号：WO2011053867A1
公开(公告)日：2011-05-05
申请号：PCT/US2010/054861
申请日：2010-10-29
申请人： AURORA ALGAE, INC. , FLEISCHER, Daniel , JUKIC, Marko , THOMPSON, Andrew , RADAELLI, Guido
发明人： FLEISCHER, Daniel , JUKIC, Marko , THOMPSON, Andrew , RADAELLI, Guido
IPC分类号： C12N1/12 , B01D12/00
CPC分类号： C11B1/10 , C11B1/106
摘要： Exemplary methods include centrifuging a wet algal biomass to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a centrifuged algal biomass, mixing the centrifuged algal biomass with an amphiphilic solvent to result in a mixture, heating the mixture to result in a dehydrated, defatted algal biomass, separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids, evaporating the amphiphilic solvent from the water and the lipids, and separating the water from the lipids. The amphiphilic solvent may be selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde. Other exemplary methods include filtering a wet algal biomass through a membrane to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a filtered algal biomass.
摘要翻译：示例性方法包括将湿藻类生物质离心以将湿藻类生物质的固体含量提高到约10％至40％之间，以产生离心的藻类生物质，将离心的藻类生物质与两亲溶剂混合以产生混合物，加热混合物导致脱水的脱脂藻类生物质，将两亲溶剂与脱水的脱脂藻类生物质分离以产生两亲溶剂，水和脂质，从水和脂质中蒸发出两亲溶剂，并将水从脂类。两亲溶剂可以选自丙酮，甲醇，乙醇，异丙醇，丁酮，二甲醚和丙醛。其他示例性方法包括通过膜过滤湿藻类生物质以将湿藻类生物质的固体含量增加至约10％至40％之间，以产生过滤的藻类生物质。

2. 发明申请

WO2009082696A1 METHODS FOR CONCENTRATING MICROALGAE 审中-公开
标题翻译：浓缩微藻的方法
公开(公告)号：WO2009082696A1
公开(公告)日：2009-07-02
申请号：PCT/US2008/087722
申请日：2008-12-19
申请人： AURORA BIOFUELS, INC. , RADAELLI, Guido , FLEISCHER, Daniel , VICK, Bertrand , CASPARI, Matthew , WEISSMAN, Joseph , RICE, David
发明人： RADAELLI, Guido , FLEISCHER, Daniel , VICK, Bertrand , CASPARI, Matthew , WEISSMAN, Joseph , RICE, David
IPC分类号： C12N1/02 , C02F1/52
CPC分类号： C12N1/02 , C02F1/5236 , C02F1/56 , C02F11/04
摘要： The present invention provides commercially viable, large-scale methods for concentrating microalgae with an average diameter of about 20 μm or less. The methods find use in concentrating microalgae with an average diameter of about 5 μm or less, for example, Nannochloropsis .
摘要翻译：本发明提供了用于浓缩平均直径为约20μm或更小的微藻的商业可行的大规模方法。该方法可用于浓缩平均直径约5μm或更小的微藻，例如，Nannochloropsis。

3. 发明申请

WO2010008490A1 THE USE OF 2-HYDROXY-5-OXOPROLINE IN CONJUNCTION WITH ALGAE 审中-公开
标题翻译：使用2-羟基-5-氧杂环戊烷与阿拉伯糖连接
公开(公告)号：WO2010008490A1
公开(公告)日：2010-01-21
申请号：PCT/US2009/003819
申请日：2009-06-25
申请人： AURORA BIOFUELS, INC. , VICK, Bertrand , FLEISCHER, Daniel
发明人： VICK, Bertrand , FLEISCHER, Daniel
IPC分类号： C07D207/28
CPC分类号： C12N1/12
摘要： Provided herein are exemplary methods for the use of 2-hydroxy-5-oxoproline in conjunction with algae. One exemplary method includes applying an effective amount of 2-hydroxy-5-oxoproline to algae in an aqueous environment to accelerate creation of a high-cell density of the algae. The effective amount of the 2-hydroxy-5-oxoproline may be approximately 0.1 grams per liter of the aqueous environment, or up to approximately 0.1 grams per liter of the aqueous environment. The effective amount of the 2-hydroxy-5-oxoproline may be applied to the aqueous environment at or near a same time, or applied to the aqueous environment over a period of time. Exemplary algae cultivation systems are also provided herein. One exemplary system includes an aqueous environment having a pale-green mutant Nannochloropsis, and an effective amount of 2-hydroxy-5-oxoproline to accelerate creation of a high-cell density of the pale-green mutant Nannochloropsis.
摘要翻译：本文提供了使用2-羟基-5-氧代脯氨酸与藻类结合使用的示例性方法。一种示例性方法包括在水性环境中向藻类施加有效量的2-羟基-5-氧代脯氨酸以加速藻类的高细胞密度的产生。 2-羟基-5-氧代脯氨酸的有效量可以为约0.1克/升的水性环境，或高达约0.1克/升的水性环境。 2-羟基-5-氧代脯氨酸的有效量可以在或同时施加到水性环境中，或者在一段时间内施加到水性环境中。本文还提供了典型的藻类培养系统。一个示例性系统包括具有淡绿色突变体Nannochloropsis的水性环境和有效量的2-羟基-5-氧代脯氨酸，以加速产生浅绿色突变体Nannochloropsis的高细胞密度。

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