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3. 发明申请

WO0185925A2 METHOD FOR THE PRODUCTION OF PROTEINS IN A YEAST OF THE GENUS ARXULA AND SUITABLE PROMOTERS 审中-公开
标题翻译：方法在类的 Arxula 和合适启动子的酵母中生产蛋白质的

公开(公告)号：WO0185925A2

公开(公告)日：2001-11-15

申请号：PCT/EP0105225

申请日：2001-05-08

申请人： RHEIN BIOTECH PROZ & PROD GMBH , INST PFLANZENGENETIK & KULTUR , KUNZE GOTTHARD , WALTER INES , ROESEL HARALD , BOEER ERIK , STOLTENBURG REGINA , WARTMANN THOMAS , DUC BUI MINH

发明人： KUNZE GOTTHARD , WALTER INES , ROESEL HARALD , BOEER ERIK , STOLTENBURG REGINA , WARTMANN THOMAS , DUC BUI MINH

IPC分类号： C12N15/81 , C12N15/00

CPC分类号： C12N15/815

摘要： The invention relates to a method for the production of one or several proteins in a host cell of the yeast genus Arxula and a nucleic acid molecule, comprising a suitable promoter. The invention further relates to an expression vector, a host cell, a kit and the use thereof. The aim of the invention is a method for the production of a protein in a yeast of the genus Arxula , by means of which large amounts of protein may be obtained. Said aim is achieved by means of a method for the production of one or several proteins in a host cell of the yeast genus Arxula , comprising: (i) cloning of at least one nucleic acid, which codes for a heterologous protein in an expression vector, containing an inducible promoter from a yeast of the genus Arxula , or a promoter from a constitutively expressed gene, chosen from the ARFC3 gene and the AHSB4 gene, from a yeast of the genus Arxula , such that the cloned nucleic acid is under the transcription control of the promoter; (ii) introduction of the expression vector obtained in (i) into a host cell of the yeast genus Arxula , suitable for the production of the heterologous protein; (iii) cultivation of the host cells obtained in (ii) and, optionally, (iv) induction of the promoter; (v) harvesting the protein by a known method.

摘要翻译：本发明涉及一种方法，用于在酵母属的宿主细胞中产生的一种或多种蛋白质的 Arxula 和一个核酸分子，其包含适用于该目的的启动子。此外，本发明涉及一种表达载体，宿主细胞，和试剂盒以及它们的用途。本发明的目的是提供用于在属的酵母生产蛋白质的方法的 Arxula 的可以以更高的量的蛋白质而获得的。这个目的是通过一种用于在酵母属的宿主细胞中产生的一种或多种蛋白质来实现的 Arxula 的包括：（i）克隆的至少一种核酸编码异源蛋白质，在表达载体中，从该属的酵母诱导型启动子的 Arxula 的或组成型表达的基因的从 ARFC3 的基因和 AHSB4 的基因选择启动子从该属的酵母含有的 Arxula 的，以使克隆的核酸在启动子的转录控制下; （ii）将在适当的（i）中获得的表达载体用于生产异源蛋白质，酵母属的宿主细胞的 Arxula 的; （iii）培养在（ii）和任选的（ⅳ）诱导启动子中获得的宿主细胞; （V）收获以常规方式的蛋白。

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