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1. 发明申请

WO0185925A2 METHOD FOR THE PRODUCTION OF PROTEINS IN A YEAST OF THE GENUS ARXULA AND SUITABLE PROMOTERS 审中-公开
标题翻译：方法在类的 Arxula 和合适启动子的酵母中生产蛋白质的

公开(公告)号：WO0185925A2

公开(公告)日：2001-11-15

申请号：PCT/EP0105225

申请日：2001-05-08

申请人： RHEIN BIOTECH PROZ & PROD GMBH , INST PFLANZENGENETIK & KULTUR , KUNZE GOTTHARD , WALTER INES , ROESEL HARALD , BOEER ERIK , STOLTENBURG REGINA , WARTMANN THOMAS , DUC BUI MINH

发明人： KUNZE GOTTHARD , WALTER INES , ROESEL HARALD , BOEER ERIK , STOLTENBURG REGINA , WARTMANN THOMAS , DUC BUI MINH

IPC分类号： C12N15/81 , C12N15/00

CPC分类号： C12N15/815

摘要： The invention relates to a method for the production of one or several proteins in a host cell of the yeast genus Arxula and a nucleic acid molecule, comprising a suitable promoter. The invention further relates to an expression vector, a host cell, a kit and the use thereof. The aim of the invention is a method for the production of a protein in a yeast of the genus Arxula , by means of which large amounts of protein may be obtained. Said aim is achieved by means of a method for the production of one or several proteins in a host cell of the yeast genus Arxula , comprising: (i) cloning of at least one nucleic acid, which codes for a heterologous protein in an expression vector, containing an inducible promoter from a yeast of the genus Arxula , or a promoter from a constitutively expressed gene, chosen from the ARFC3 gene and the AHSB4 gene, from a yeast of the genus Arxula , such that the cloned nucleic acid is under the transcription control of the promoter; (ii) introduction of the expression vector obtained in (i) into a host cell of the yeast genus Arxula , suitable for the production of the heterologous protein; (iii) cultivation of the host cells obtained in (ii) and, optionally, (iv) induction of the promoter; (v) harvesting the protein by a known method.

摘要翻译：本发明涉及一种方法，用于在酵母属的宿主细胞中产生的一种或多种蛋白质的 Arxula 和一个核酸分子，其包含适用于该目的的启动子。此外，本发明涉及一种表达载体，宿主细胞，和试剂盒以及它们的用途。本发明的目的是提供用于在属的酵母生产蛋白质的方法的 Arxula 的可以以更高的量的蛋白质而获得的。这个目的是通过一种用于在酵母属的宿主细胞中产生的一种或多种蛋白质来实现的 Arxula 的包括：（i）克隆的至少一种核酸编码异源蛋白质，在表达载体中，从该属的酵母诱导型启动子的 Arxula 的或组成型表达的基因的从 ARFC3 的基因和 AHSB4 的基因选择启动子从该属的酵母含有的 Arxula 的，以使克隆的核酸在启动子的转录控制下; （ii）将在适当的（i）中获得的表达载体用于生产异源蛋白质，酵母属的宿主细胞的 Arxula 的; （iii）培养在（ii）和任选的（ⅳ）诱导启动子中获得的宿主细胞; （V）收获以常规方式的蛋白。

2. 发明申请

WO9617068A3 PATHOGEN-RESISTANT PLANTS AND METHOD FOR PRODUCINGPATHOGEN-RESISTANT PLANTS AND METHOD FOR PRODUCING THEM THEM 审中-公开
标题翻译：耐病植物和耐病植物生产方法及其生产方法
公开(公告)号：WO9617068A3
公开(公告)日：1996-08-29
申请号：PCT/DE9501698
申请日：1995-11-21
申请人： INST PFLANZENGENETIK & KULTUR , SONNEWALD UWE , HERBERS KARIN
发明人： SONNEWALD UWE , HERBERS KARIN
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , A01H5/00 , A01N65/00
CPC分类号： C12N15/8283 , C07K14/415
摘要： The invention pertains to the isolation of new pathogen-induced cDNA clones as well as a method for producing pathogen-resistant plants, wherein a chimeric gene construct consisting of an active promoter, a region coding virus-induced proteins (VIP proteins) and a polyadenylation signal is introduced into the genetic material of the plant. The new VIP protein genes were isolated from transgenic tobacco plants that have an elevated resistance to virus. The VIP proteins represent a new class of PR proteins. The invention also pertains to new DNA sequences which code for the VIP proteins as well as plants and parts of plants that have genetic material altered using the claimed method. The new plants have a higher resistance to pathogens. The invention has industrial application in agriculture and genetic engineering.

3. 发明申请

WO0175128A3 METHOD FOR THE PRODUCTION OF LEGUMINOUS PLANTS WITH INCREASED PROTEIN CONTENT AND LONGER SEED FILLING DURATION 审中-公开
标题翻译：用于生产LEGUMES随着对长时间种子填充增加蛋白质
公开(公告)号：WO0175128A3
公开(公告)日：2002-04-04
申请号：PCT/DE0101208
申请日：2001-03-23
申请人： INST PFLANZENGENETIK & KULTUR , WEBER HANS , SAALBACH ISOLDE , GIERSBERG MARTIN , HOFFMEISTER PETRA
发明人： WEBER HANS , SAALBACH ISOLDE , GIERSBERG MARTIN , HOFFMEISTER PETRA
IPC分类号： C12N9/90 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8205 , C12N9/90 , C12N15/8245 , C12N15/8251
摘要： The invention relates to a method for the production of leguminous plants with increased protein content in the seeds and longer seed filling duration, by means of introduction of recombinant DNA molecules. Said recombinant DNA molecules are introduced into the plant, by means of a transformation system and comprise a DNA sequence from the plant, expressed in plants, the genetic product of which inhibits a protein in the seed with the enzymatic activity of an ADP glucose pyrophosphorylase (AGP) and/or a plastid phosphoglucomutase (pPGM) and, optionally, the regulatory sequence of a seed-specific promoter in leguminous plants. Furthermore, at least one selection marker gene is separately transferred, which is subsequently removed again. The plants which display an increased protein content and a lengthier seed-filling duration are chosen.
摘要翻译：本发明涉及一种用于与在种子填充期间通过导入重组DNA分子的延伸在种子提高的蛋白质含量豆类的制备方法。在植物，所述重组DNA分子通过转化系统，而引入其中它们包括一个特定的植物DNA序列，其被带入植物中表达，在具有ADP葡萄糖焦磷酸化酶（AGP）的酶活性的蛋白质的种子其基因产物和 /或质体葡萄糖磷酸（pPGM）抑制和在豆类种子特异性启动子的可能的调控序列。此外，可以单独地发送的至少一个选择标记基因，其随后被再次移除。该系统将选择具有增加的蛋白质含量和更长的种子填充期间的植物。

4. 发明申请

WO0026388A2 NOVEL EXPRESSION CASSETTE FOR EXPRESSING GENES IN PLANT SEED 审中-公开
标题翻译：新的表达盒用于表达植物种子中的任何基因
公开(公告)号：WO0026388A2
公开(公告)日：2000-05-11
申请号：PCT/DE9903432
申请日：1999-10-27
申请人： INST PFLANZENGENETIK & KULTUR , HEIM UTE , WEBER HANS
发明人： HEIM UTE , WEBER HANS
IPC分类号： A01H5/00 , C07K14/415 , C12N5/10 , C12N15/09 , C12N15/82 , C12N15/63
CPC分类号： C12N15/8234 , C07K14/415
摘要： The invention relates to an expression cassette for expressing genes in plant seed and to the plasmids containing said expression cassette. The invention includes the production of transgenic plant cells containing said expression cassette and the use of the plasmids in said expression cassette for producing transgenic plants. The invention can be applied in the field of biotechnology, pharmaceutics and plant production. The aim of the invention is to provide a means for the seed-specific expression in transgenic plants in such a manner that it is suitable for the production of the desired substances. Another aim of the invention is to construct an expression cassette which allows stable expression of genes of substances to be produced in plant seed at a high expression rate. The inventive expression cassette comprises the following essential components: the promoter of the gene of the seed protein which is analogous to the sucrose binding protein (SBP), optionally the DNA sequence of a signal peptide, preferably that of the SBP signal peptide, a gene to be expressed, 3' termination sequences.
摘要翻译：本发明涉及用于在植物种子中表达任何基因的表达盒和含有该表达盒的质粒。本发明包括含有该表达盒的转基因植物细胞的制备和该表达盒中质粒在生产转基因植物中的用途。本发明的应用领域是生物技术，制药和植物生产。本发明的目的是在适合物质生产的基础上在转基因植物中提供种子特异性表达。它基于构建表达盒的目的，该表达盒可以实现植物种子中待生产物质基因的高表达率的稳定表达。本发明的表达盒含有以下基本组分：蔗糖结合蛋白（SBP）样种子蛋白基因的启动子; 任选的信号肽的DNA序列，优选SBP信号肽; 待表达的基因; 3“终止序列。

5. 发明申请

WO9960107A2 NICOTIANAMINE SYNTHASE GENES, ISOLATION AND USE THEREOF 审中-公开
标题翻译： NICO安·阿明合酶基因及其分离及用途
公开(公告)号：WO9960107A2
公开(公告)日：1999-11-25
申请号：PCT/DE9901585
申请日：1999-05-18
申请人： INST PFLANZENGENETIK & KULTUR , BAEUMLEIN HELMUT , GANAL MARTIN , HERBIK ALEXANDRA , LING HONG QING , MOCK HANS PETER , STEPHAN UDO
发明人： BAEUMLEIN HELMUT , GANAL MARTIN , HERBIK ALEXANDRA , LING HONG-QING , MOCK HANS-PETER , STEPHAN UDO
IPC分类号： C12N9/10 , C12N15/60 , C12N15/82 , C12N15/00
CPC分类号： C12N9/1085 , C12N15/8242 , C12N15/8243 , C12N15/8261 , Y02A40/146
摘要： The invention relates to nicotianamine synthase genes, the isolation and use thereof. The invention can be used in agriculture and environmental protection. According to the invention, nicotianamine synthases and the genes coding for the latter are isolated and sequenced. Special embodiment examples are based on isolation from barley or tomatoes.
摘要翻译：本发明涉及烟酰胺合酶基因，它们的分离和它们的用途。本发明的应用是农业和环保。根据本发明，nicotianamin合酶和编码基因它们分离并测序。具体实施方式基于来自大麦或从番茄中分离。

6. 发明申请

WO9854304A3 CHITINASE 审中-公开
标题翻译：几丁质酶
公开(公告)号：WO9854304A3
公开(公告)日：1999-03-04
申请号：PCT/DE9801497
申请日：1998-05-27
申请人： INST PFLANZENGENETIK & KULTUR , SCHLESIER BERNHARD , WOLF ANNEGRET , KOCH GUDRUN , HORSTMANN CHRISTIAN , KOENIG SUSANNE , GILLANDT ROSE MARIE
发明人： SCHLESIER BERNHARD , WOLF ANNEGRET , KOCH GUDRUN , HORSTMANN CHRISTIAN , KOENIG SUSANNE , GILLANDT ROSE-MARIE
IPC分类号： C12N9/24 , C12P19/26
CPC分类号： C12Y302/01014 , C12N9/2442 , C12P19/26
摘要： The invention relates to a novel chitinase, its production and use. Areas of application of the invention are medicine and the food industry. The novel chitinase is produced from the seed of the giant bean Canavalia ensiformis (L.) DC.). It is characterized by a molecular weight of 26225, an isoelectric point pH 9.3 and an optimum pH for fission of chitin pH 6.0, as well as by the sequence (a).
摘要翻译：本发明涉及新几丁质酶，其生产和使用。本发明的应用领域是医药和食品工业。新颖的几丁质酶是由巨豆Canavalia ensiformis（L.）DC的种子生产的）。其特征在于分子量为26225，等电点pH 9.3，以及几丁质pH6.0裂解的最适pH以及序列（a）。

7. 发明申请

WO9821336A3 Method for genetic control of seed maturation 审中-公开
标题翻译：一种用于种子成熟的植物遗传控制过程
公开(公告)号：WO9821336A3
公开(公告)日：1998-07-16
申请号：PCT/DE9702617
申请日：1997-11-05
申请人： INST PFLANZENGENETIK & KULTUR , LUERSSEN HARTMUT , MISERA SIMON
发明人： LUERSSEN HARTMUT , MISERA SIMON
IPC分类号： A01H5/00 , C07K14/415 , C12N15/09 , C12N15/29 , C12N15/82 , A01H5/10
CPC分类号： C07K14/415 , C12N15/8293
摘要： The aim of the invention is to provide genetic control of seed maturation and seed dormancy by transferring regulating genes into plants. The method used to acheive this is characterized by: stable integration of DNA into the genome of a plant cell; said DNA coding for a protein which, in a region of 107 amino acids, corresponds to the FUS3 protein of arabidopsis thaliana in at least 50 % of the amino acids; constituent or inducible expression of DNA with suitable elements controlling transcription, and regeneration of plants from transgenic cells.

8. 发明申请

WO9701567A2 MICROSATELLITE MARKERS FOR PLANTS OF THE SPECIES TRITICUM AESTIVUM AND TRIBE TRITICEAE AND THE USE OF SAID MARKERS 审中-公开
标题翻译：微小卫星MARKER对植物物种小麦和小麦TRIBUS及其用途
公开(公告)号：WO9701567A2
公开(公告)日：1997-01-16
申请号：PCT/DE9601185
申请日：1996-06-27
申请人： INST PFLANZENGENETIK & KULTUR , ROEDER MARION , PLASCHKE JENS , GANAL MARTIN
发明人： ROEDER MARION , PLASCHKE JENS , GANAL MARTIN
IPC分类号： C12Q1/68 , C07H
CPC分类号： C12Q1/6876 , C12Q1/6858 , C12Q1/6895 , C12Q2600/156
摘要： The invention pertains to novel genetic markers for wheats (Triticum aestivumL.) and closely related species (tribe: Triticeae) and to the use of these markers. The aim of the invention is to develop novel microsatellite markers intended for use in the genetic analysis of plants of the species Triticum aestivum and characterised by a higher level of DNA polymorphism than other molecular probes hitherto developed for the wheat genome. The invention involves the preparation of 233 microsatellite markers, or a proportion thereof, which are defined as primer pairs with associated microsatellite sequences and amplify loci of various chromosomes of the wheat genome and are therefore suitable for use in genetic marking.
摘要翻译：本发明涉及一种用于小麦的新的遗传标记物（小麦aestivumL。）和密切相关的物种（部落：小麦）和它们的用途。本发明的目的是提供用于该物种小麦的植物，其特征在于较高程度的DNA多态性的遗传分析提供了新的微卫星标记作为其它分子探针开发迄今用于小麦基因组中。根据本发明的233个微卫星标记，或者其数量，提供，其被定义为引物对与相关联的微卫星序列和扩增小麦基因组的不同的染色体上的基因座，其和因此适合的基因标记。

9. 发明申请

WO2006002955A3 POLYPEPTIDES HAVING TANNASE AND/OR LIPASE ACTIVITY 审中-公开
标题翻译：多肽鞣酸和/或脂肪酶活性
公开(公告)号：WO2006002955A3
公开(公告)日：2006-04-20
申请号：PCT/EP2005007133
申请日：2005-07-01
申请人： INST PFLANZENGENETIK & KULTUR , BOER ERIK , KUNZE GOTTHARD
发明人： BOER ERIK , KUNZE GOTTHARD
IPC分类号： C12N9/18 , C12N9/20
CPC分类号： C12N9/18 , C12N9/20
摘要： The invention relates to nucleic acids and proteins coded therefrom which have a comparatively very high tannase activity and/or lipase activity. The tannases are provided with a broad substrate spectrum and crack both condensed and hydrolyzable tannins. The inventive tannases are easy to produce, thus allowing the expenses for using tannases in industrial applications to be reduced.
摘要翻译：本发明涉及一种机动车辆用的包括连续长条座椅至少一排和两部分的靠背。为了提供在其中一个舒适的座位设置在第三排座椅，其即使对于高个子的人提供了在处置的充分的空间的中间设置一个机动车辆，本发明提供使座椅（3a）的在其中央区域降低。

10. 发明申请

WO03004659A8 RECOMBINATION SYSTEMS AND A METHOD FOR REMOVING NUCLEIC ACID SEQUENCES FROM THE GENOME OF EUKARYOTIC ORGANISMS 审中-公开
标题翻译：重组和除去方法NUCLEIC序列从基因组真核生物
公开(公告)号：WO03004659A8
公开(公告)日：2004-04-22
申请号：PCT/EP0207281
申请日：2002-07-02
申请人： SUNGENE GMBH & CO KGAA , INST PFLANZENGENETIK & KULTUR , PUCHTA HOLGER , BIESGEN CHRISTIAN
发明人： PUCHTA HOLGER , BIESGEN CHRISTIAN
IPC分类号： A01H5/00 , A01K67/027 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/82 , C12N15/90 , C12N5/00
CPC分类号： C12N15/8213 , C12N15/90
摘要： The invention relates to recombination systems and to a method for removing nucleic acid sequences from the chromosomal DNA of eukaryotic organisms. The invention also relates to transgenic organisms (preferably plants), containing said systems or produced by said method.
摘要翻译：本发明涉及重组的系统和方法用于从真核生物，和转基因生物的染色体DNA中除去的核酸序列 - 优选植物 - 含有这些系统或采用该方法制备的。

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