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1. 发明公开

EP1829968A4 MUTANT PROTEIN HAVING PEPTIDE-PRODUCTION ACTIVITY 审中-公开
标题翻译：用肽生产活动蛋白突变体
公开(公告)号：EP1829968A4
公开(公告)日：2008-09-10
申请号：EP05820283
申请日：2005-12-20
申请人： AJINOMOTO KK
发明人： ABE ISAO , TAKESHITA RIE , HARA SEIICHI , SUZUKI SONOKO , YOKOZEKI KENZO , SUGIYAMA MASAKAZU , SUZUKI SHUNICHI , WATANABE KUNIHIKO , SHIMBA NOBUHISA , NAKAMURA TAKEFUMI , TAGAMI UNO , KODAMA YUYA , ONOYE HIROMI , YUUJI REIKO , SUZUKI EIICHIRO , KASHIWAGI TATSUKI , XU NINGCHUN , KAI YUKO
IPC分类号： C12N15/09 , C12N9/00 , C12P13/00 , C12P21/02
CPC分类号： C07K14/00 , C07K14/195 , C12N9/93
摘要： Disclosed is an excellent peptide-producing protein. Also disclosed is a process for producing a peptide with high efficiency, or the like. The process comprises reacting an amine component with a carboxy component in the presence of at least one of the following proteins (I) and (II) to produce a peptide: (I) a mutant protein comprising an amino acid sequence having one or more of the mutations shown in mutation types 1 to 68, 239 to 290 and 324 to 377 in the amino acid sequence depicted in SEQ ID NO: 2; and (II) a mutant protein comprising an amino acid sequence having one or more of the mutations shown in mutation types L1 to L335 and M1 to M642 in the amino acid sequence depicted in SEQ ID NO: 208.

2. 发明公开

EP2479272A4 METHOD FOR PRODUCING MONATIN 审中-公开
标题翻译：工艺生产莫纳甜
公开(公告)号：EP2479272A4
公开(公告)日：2013-11-20
申请号：EP11820789
申请日：2011-10-12
申请人： AJINOMOTO KK
发明人： TAKAKURA YASUAKI , OGINO HIROOMI , SUGIYAMA MASAKAZU , MORI KENICHI , TABUCHI ERI , ISHIKAWA KOKI , TAGAMI UNO , FUJII HIDEMI
IPC分类号： C12P17/10 , C12N9/10 , C12P41/00
CPC分类号： C12P17/10 , C12N9/1096 , C12P41/006 , C12Y206/01 , C12Y206/01001 , C12Y401/01003
摘要： The present invention provides a methodology for improving a yield of 2R, 4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S, 4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S, 4R-Monatin to form the 2R, 4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.

3. 发明公开

EP3072967A4 MODIFIED ISOPRENE SYNTHASE 有权
标题翻译： MODIFIZIERTE ISOPRENSYNTHASE
公开(公告)号：EP3072967A4
公开(公告)日：2017-06-14
申请号：EP14864940
申请日：2014-11-21
申请人： AJINOMOTO KK
发明人： NAKATA KUNIO , TAJIMA YOSHINORI , TAGAMI UNO , OKU TAKASHI , KASHIMA YASUHIRO
IPC分类号： C12N9/88 , C08F36/08 , C12P5/02
CPC分类号： C12N9/88 , C08F36/08 , C08K3/04 , C08K3/22 , C08K2003/2296 , C12N15/09 , C12P5/007 , C12P5/026 , C12Y402/03007 , C12Y402/03027 , C08L9/00
摘要： The present invention provides an enzyme and the like useful for establishing an excellent isoprene monomer production system. Specifically, the present invention provides a modified isoprene synthase that has a mutation(s) of a given amino acid residue(s) in any amino acid sequence of (a) the amino acid sequence of SEQ ID NO:4, (b) an amino acid sequence having one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NO:4, or (c) an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:4, and has an isoprene synthetic activity; a polynucleotide encoding the modified isoprene synthase; a vector comprising the polynucleotide; a host cell comprising an expression unit comprising the polynucleotide; and a method for producing an isoprene monomer comprising producing the isoprene monomer from dimethylallyl diphosphate in the presence of the modified isoprene synthase.
摘要翻译：本发明提供了用于建立优异的异戊二烯单体生产体系的酶等。具体而言，本发明提供了在（a）氨基酸序列SEQ ID NO：4的任何氨基酸序列中具有给定氨基酸残基突变的修饰异戊二烯合酶，（b）在氨基酸序列SEQ ID NO：4中具有一个或几个氨基酸取代，缺失，插入或添加的氨基酸序列，或（c）与SEQ ID NO：4的氨基酸序列具有90％或更多同一性的氨基酸序列 NO：4，具有异戊二烯合成活性; 编码修饰的异戊二烯合酶的多核苷酸; 包含该多核苷酸的载体; 包含含有所述多核苷酸的表达单元的宿主细胞; 以及生产异戊二烯单体的方法，包括在改性异戊二烯合酶存在下由二甲基烯丙基二磷酸酯生产异戊二烯单体。

4. 发明公开

EP3101130A4 MUTANT GLUTAMATE-CYSTEINE LIGASE AND METHOD FOR MANUFACTURING GAMMA-GLUTAMYL-VALYL-GLYCINE 有权
公开(公告)号：EP3101130A4
公开(公告)日：2017-11-29
申请号：EP15743180
申请日：2015-01-30
申请人： AJINOMOTO KK
发明人： SASAHARA AYAKO , TABUCHI ERI , SUZUKI HIDEYUKI , TAGAMI UNO , KASHIWAGI TATSUKI , ITO TAKAYUKI , NOZAKI HIROYUKI , SUZUKI SHUNICHI
IPC分类号： C12N15/09 , C12N1/21 , C12N9/00 , C12P21/02
CPC分类号： C12N9/93 , C07K5/0215 , C12P21/02 , C12Y603/02002
摘要： A mutant glutamate-cysteine ligase (GSHA) suitable for generating ³-Glu-Val, and a method for producing ³-Glu-Val-Gly using the same are provided. ³-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and ³-Glu-Val-Gly is further produced by using ³-Glu-Val and Gly as raw materials. ³-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.

5. 发明专利

AU2018334768A1 Protein production method and disaccharide production method 未知
公开(公告)号：AU2018334768A1
公开(公告)日：2020-04-16
申请号：AU2018334768
申请日：2018-09-25
申请人： AJINOMOTO KK
发明人： YAHAGI DAIKI , YOSHIDA ERIKA , FUKADA HIROAKI , TOKURA MITSUNORI , TAGAMI UNO , SUGIKI MASAYUKI
IPC分类号： C12N15/09 , C12N9/42 , C12N15/55 , C12P19/12 , C12P21/02
摘要： Provided is a method for producing a target protein and a method for producing a disaccharide. A target protein is produced by culturing Talaromyces cellulolyticus in a medium containing an expression-inducing substance such as gentiobiose. A disaccharide is produced by enzymic conversion from a sugar material using a disaccharide synthesizing enzyme.

6. 发明专利

RU2505606C2 СПОСОБ ПОЛУЧЕНИЯ МОНАТИНА 未知
公开(公告)号：RU2505606C2
公开(公告)日：2014-01-27
申请号：RU2012110249
申请日：2011-10-12
申请人： AJINOMOTO KK
发明人： TAKAKURA JASUAKI , OGINO KHIROOMI , SUGIJAMA MASAKADZU , MORI KENITI , TABUTI EHRI , ISIKAVA KOKI , TAGAMI UNO , FUDZII KHIDEMI
IPC分类号： C12P13/00 , C12N15/09
摘要： Настоящееизобретениеотноситсяк областибиохимии, ипредставляетсобойспособполучения 2S,4R-монатинаилиегосоли, предусматривающийконтактирование 4R-IHOG саминотрансферазой L-аминокислотыв присутствии L-аминокислоты, испособполучения 2R,4R-монатинаилиегосоли, предусматривающийизомеризацию 2S,4R-монатина. Такженастоящееизобретениераскрываетаминотрансферазы L-аминокислотыдляосуществленияспособовполучениямонатинаи способполучениятаких L-аминотрансфераз, предусматривающийкультивированиебактериальнойклетки, вкоторуювведенвекторэкспрессии, содержащийполинуклеотид, кодирующийпредставленнуюаминотрансферазу. Настоящееизобретениепозволяетполучить 2S,4R-монатини 2R,4R-монатинс улучшеннымвыходомблагодаряиспользованиюраскрытыхаминотрансфераз L-аминокислоты. 9 н. и 18 з.п. ф-лы, 6 ил., 23 табл., 48 пр.

7. 发明专利

CA2769606A1 METHOD FOR PRODUCING MONATIN 未知
公开(公告)号：CA2769606A1
公开(公告)日：2012-04-14
申请号：CA2769606
申请日：2011-10-12
申请人： AJINOMOTO KK
发明人： TAKAKURA YASUAKI , OGINO HIROOMI , SUGIYAMA MASAKUZU , MORI KENICHI , TABUCHI ERI , ISHIKAWA KOKI , TAGAMI UNO , FUJII HIDEMI
IPC分类号： C12N15/54 , C12N9/10 , C12P13/04 , C12P13/22 , C12P17/08
摘要： The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.

8. 发明专利

CA2592309A1 MUTANT PROTEIN HAVING PEPTIDE-PRODUCTION ACTIVITY 未知
公开(公告)号：CA2592309A1
公开(公告)日：2006-07-20
申请号：CA2592309
申请日：2005-12-20
申请人： AJINOMOTO KK
发明人： SUGIYAMA MASAKAZU , ABE ISAO , SUZUKI EIICHIRO , YUUJI REIKO , KODAMA YUYA , SUZUKI SHUNICHI , KASHIWAGI TATSUKI , NAKAMURA TAKEFUMI , TAKESHITA RIE , SHIMBA NOBUHISA , XU NINGCHUN , KAI YUKO , HARA SEIICHI , WATANABE KUNIHIKO , TAGAMI UNO , ONOYE HIROMI , SUZUKI SONOKO , YOKOZEKI KENZO
IPC分类号： C12N15/09 , C12N9/00 , C12P13/00 , C12P21/02
摘要： The present invention aims at providing an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. The peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II). (I) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations 1 to 68, and the mutations 239 to 290 and 324 to 377 in an amino acid sequence of SEQ ID NO:2. (II) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations L1 to L335 and M1 to M642 in an amino acid sequence of SEQ ID NO:208

9. 发明专利

SG11201808590TA FIBROIN-LIKE PROTEIN VARIANT AND CELL CULTURE METHOD 未知
公开(公告)号：SG11201808590TA
公开(公告)日：2018-10-30
申请号：SG11201808590T
申请日：2017-03-31
申请人： AJINOMOTO KK
发明人： KITAZAWA MANABU , SUGIMOTO NAO , KODAMA RIHO , NAGAHIKO TAKESHI , ITO YOSHIHIRO , NAKASE KENTARO , TAKASHIMA YOSHINORI , MINE AYAKO , KUWABARA YOKO , TAGAMI UNO , SUGIKI MASAYUKI
IPC分类号： C12N15/09 , C07K14/435 , C07K19/00 , C12M3/04 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/0735 , C12N5/10
摘要： FIBROIN-LIKE PROTEIN VARIANT AND CELL CULTURE METHOD A technique for efficiently culturing cells such as stem cells is provided. Cells such as stem cells are cultured using a cell culture vessel coated with a fibroin-like protein inserted with a cell adhesion sequence containing RGD (Arg-Gly-Asp). FIG. 1

10. 发明专利

CA3019325A1 FIBROIN-LIKE PROTEIN VARIANT AND CELL CULTURE METHOD 未知
公开(公告)号：CA3019325A1
公开(公告)日：2017-10-05
申请号：CA3019325
申请日：2017-03-31
申请人： AJINOMOTO KK
发明人： KITAZAWA MANABU , SUGIMOTO NAO , KODAMA RIHO , NAGAHIKO TAKESHI , ITO YOSHIHIRO , NAKASE KENTARO , TAKASHIMA YOSHINORI , MINE AYAKO , KUWABARA YOKO , TAGAMI UNO , SUGIKI MASAYUKI
IPC分类号： C12N15/09 , C07K14/435 , C07K19/00 , C12M3/04 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/0735 , C12N5/10
摘要： Provided is a technique for efficiently culturing cells such as stem cells. Cells such as stem cells are cultured with the use of a cell culture container coated with a fibroin-like protein which carries an RGD (Arg-Gly-Asp)-containing cell adhesion sequence inserted thereinto.

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