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1. 发明公开

EP1241246A4 PROCESS FOR PRODUCING L-AMINO ACID AND NOVEL GENE 有权
标题翻译： VERFAHREN ZUR HERSTELLUNG VON L-AMINOSÄUREUND NEUES GEN
公开(公告)号：EP1241246A4
公开(公告)日：2004-03-24
申请号：EP00985850
申请日：2000-12-22
申请人： AJINOMOTO KK
发明人： SUGIMOTO MASAKAZU , NAKAI YUTA , ITO HISAO , KURAHASHI OSAMU
IPC分类号： C12N1/21 , C12N9/12 , C12N15/54 , C12P13/08 , C12P13/14 , C12N1/20 , C12P13/04 , C12P13/06 , C12P13/18 , C12R1/13 , C12R1/15
CPC分类号： C12N9/1205 , C12P13/08 , C12P13/14
摘要： A gene encoding fructose phosphotransferase is transferred into a coryneform bacterium capable of producing an L-amino acid such as L-lysine or L-glutamic acid and thus the fructose phosphotransferase activity is potentiated, thereby improving the L-amino acid productivity.
摘要翻译：将编码果糖磷酸转移酶的基因导入具有产生L-赖氨酸或L-谷氨酸等L-氨基酸的能力的棒状细菌，以提高果糖磷酸转移酶活性，从而提高L-氨基酸产生能力。

2. 发明公开

EP1197555A4 DNA ENCODING SUCROSE PTS ENZYME II 有权
标题翻译： FÜRDAS SACCHAROSE-PTS-ENZYM KODIERENDE DNA
公开(公告)号：EP1197555A4
公开(公告)日：2003-07-09
申请号：EP00940903
申请日：2000-06-30
申请人： AJINOMOTO KK
发明人： IZUI MASAKO , SUGIMOTO MASAKAZU , NAKAMATSU TSUYOSHI , KURAHASHI OSAMU
IPC分类号： C12N9/12 , C12N15/54
CPC分类号： C12N9/1205 , C12Y207/01069
摘要： A gene encoding a protein constituting sucrose PTS of a coryneform bacterium provided by amplifying the downstream domain of a coryneform bacterium sucrase gene by the cassette-ligation mediated PCR method and thus obtaining a DNA encoding sucrose PTS enzyme II which is the protein (A) or (B) described below: (A) a protein having the amino acid sequence represented by SEQ ID NO:2 in Sequence Listing; and (B) a protein having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO:2 in Sequence Listing by substitution, deletion, insertion, addition or inversion of one or more amino acids and having an activity of binding to sucrose.
摘要翻译：通过盒式连接介导的PCR扩增棒状杆菌型细菌的蔗糖酶基因下游的区域，得到编码构成棒状细菌的蔗糖PTS的蛋白质的基因，得到编码下述定义的蛋白质的蔗糖PTS酶II的DNA（ A）或（B）：（A）序列表中具有SEQ ID NO：2的氨基酸序列的蛋白质。（B）序列表中具有SEQ ID NO：2的氨基酸序列的蛋白质，包括一个或几个氨基酸的取代，缺失，插入，添加或转化，以及与蔗糖结合的活性。

3. 发明公开

EP0699759A4 VARIANT ASPARTOKINASE GENE 失效
标题翻译： ASPERTOKINASEGENVARIANTEN
公开(公告)号：EP0699759A4
公开(公告)日：1999-09-22
申请号：EP94902116
申请日：1993-12-14
申请人： AJINOMOTO KK
发明人： SUGIMOTO MASAKAZU , OGAWA YURI , SUZUKI TOMOTO , TANAKA AKIKO , MATSUI HIROSHI
IPC分类号： C12N9/12 , C12N15/09 , C12N15/54 , C12P13/08 , C12R1/13 , C12R1/15 , C12N1/11
CPC分类号： C12N9/1217 , C12N9/12 , C12P13/08
摘要： A transformant with an increased rate of L-lysine production and secretion is obtained by introducing into a corynebacterium a recombinant DNA that can be replicated in a corynebacterium and contains either a DNA fragment originating in a corynebacterium and coding for an aspartokinase α-subunit protein whereby a synergistic feedback inhibition by L-lysine and L-threonine is released or a DNA fragment originating in a corynebacterium and coding for an aspartokinase β-subunit protein whereby a synergistic feedback inhibition by L-lysine and L-threonine is released. The obtained transformant is cultured in a suitable medium and the formed L-lysine is separated.
摘要翻译：具有增加的L-赖氨酸产生和分泌速率的转化体通过将能够在棒状杆菌中复制并包含来自棒状杆菌的DNA片段和编码天冬氨酸激酶α-亚基蛋白的重组DNA导入棒状杆菌获得，由此释放L-赖氨酸和L-苏氨酸的协同反馈抑制作用或源自棒状杆菌并编码天冬氨酸激酶β-亚基蛋白的DNA片段，由此释放L-赖氨酸和L-苏氨酸的协同反馈抑制。将获得的转化体在合适的培养基中培养，分离形成的L-赖氨酸。

4. 发明公开

EP0754756A4 PROCESS FOR PRODUCING L-LYSINE 失效
标题翻译： PROCESS用于L-赖氨酸生产
公开(公告)号：EP0754756A4
公开(公告)日：1997-12-03
申请号：EP95909969
申请日：1995-02-23
申请人： AJINOMOTO KK
发明人： SUGIMOTO MASAKAZU , USUDA YOSHIHIRO , SUZUKI TOMOKO , TANAKA AKIKO , MATSUI HIROSHI
IPC分类号： C12N9/02 , C12N9/12 , C12N15/53 , C12P13/08
CPC分类号： C12N9/0004 , C12N9/1217 , C12P13/08
摘要： A coryneform bacterium having a high L-lysine productivity is provided by integrating a gene coding for a coryneform-origin aspartokinase released of feedback inhibition caused by L-lysine and L-threonine into a DNA of a chromosome of a coryneform bacterium carrying attenuated homoserine dehydrogenase or a coryneform bacterium deficient in a homoserine dehydrogenase gene.

5. 发明公开

EP0723011A4 VARIANT PHOSPHOENOLPYRUVATE CARBOXYLASE, GENE THEREOF, AND PROCESS FOR PRODUCING AMINO ACID 失效
标题翻译：磷酸烯醇式丙酮酸CARBOXYLASEVARIANTE您的GEN及其制造方法氨基
公开(公告)号：EP0723011A4
公开(公告)日：1996-05-21
申请号：EP94924384
申请日：1994-08-17
申请人： AJINOMOTO KK
发明人： SUGIMOTO MASAKAZU , SUZUKI TOMOKO , MATSUI HIROSHI , IZUI KATSURA
IPC分类号： C12N9/88 , C12P13/04 , C12P13/06 , C12P13/08 , C12P13/14 , C12P13/20
CPC分类号： C12P13/08 , C12N9/88 , C12P13/04 , C12P13/06 , C12P13/14 , C12P13/20
摘要： A variant phosphoenolpyruvate carboxylase that is not substantially inhibited by aspartic acid is produced by introducing a variant phosphoenolpyruvate carboxylase gene, such as one wherein the 625th glutamic acid residue from the N-terminus of the carboxylase has been replaced by a lysine residue or one wherein the 438th arginine residue has been replaced by a cysteine residue, into Escherichia coli or a coryneform bacterium. An amino acid can efficiently be produced by using this carboxylase.

6. 发明公开

EP0841395A4 PROCESS FOR PRODUCING L-LYSINE 失效
标题翻译： PROCESS用于L-赖氨酸生产
公开(公告)号：EP0841395A4
公开(公告)日：2005-07-27
申请号：EP96916305
申请日：1996-06-05
申请人： AJINOMOTO KK
发明人： OTSUNA SEIKO , SUGIMOTO MASAKAZU , IZUI MASAKO , HAYAKAWA ATSUSHI , NAKANO EIICHI , KOBAYASHI MASAKI , YOSHIHARA YASUHIKO , NAKAMATSU TSUYOSHI
IPC分类号： C12N1/21 , C12N9/06 , C12N9/12 , C12N9/88 , C12N15/31 , C12N15/52 , C12N15/77 , C12P13/08 , C12P19/34 , C12R1/15
CPC分类号： C12N9/0016 , C12N9/1217 , C12N9/88 , C12N15/52 , C12N15/77 , C12P13/08
摘要： L-Lysine productivity and production rate are improved by sustaining in corynebacteria the aspartokinase wherein the feedback inhibition by L-lysine and L-threonine has been substantially remitted and successively potentiating a DNA encoding dihydrodipicolinate reductase, a DNA encoding dihydrodipicolinate synthetase, a DNA encoding diaminopimelate decarboxylase and a DNA encoding diaminopimelate dehydrogenase.

7. 发明公开

EP1158043A4 PROCESS FOR PRODUCING L-LYSINE 有权
标题翻译： VERFAHREN ZUR HERSTELLUNG VON L-LYSIN
公开(公告)号：EP1158043A4
公开(公告)日：2002-05-22
申请号：EP00907974
申请日：2000-03-09
申请人： AJINOMOTO KK
发明人： ARAKI MASAYUKI , SUGIMOTO MASAKAZU , KIMURA EIICHIRO , NAKAMATSU TSUYOSHI
IPC分类号： C12N15/09 , A23K1/16 , C12N1/20 , C12N1/21 , C12N9/06 , C12P13/08 , C12N15/00
CPC分类号： C12N9/0016 , C12P13/08 , C12R1/15
摘要： A gene encoding glutamate dehydrogenase is transferred into a coryneform bacterium having an L-lysine productivity and thus the glutamate dehydrogenase activity in the cells is potentiated, thereby elevating the L-lysine productivity.
摘要翻译：将编码谷氨酸脱氢酶的基因导入具有L-赖氨酸生产力的棒状细菌以增加谷氨酸脱氢酶的细胞内活性，从而提高产生L-赖氨酸的能力。

8. 发明公开

EP0811682A3 Method of producing L-lysine 失效
标题翻译： Verfahren zur Herstellung von L-Lysin
公开(公告)号：EP0811682A3
公开(公告)日：1998-06-10
申请号：EP97108764
申请日：1997-06-02
申请人： AJINOMOTO KK
发明人： HIRANO SEIKO , SUGIMOTO MASAKAZU , NAKANO EIICHI , IZUI MASAKO , HAYAKAWA ATSUSHI , YOSHIHARA YASUHIKO , NAKAMATSU TSUYOSHI
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N9/06 , C12N9/88 , C12N15/77 , C12P13/08 , C12R1/13
CPC分类号： C12N9/0016 , C12N9/88 , C12N15/77 , C12P13/08
摘要： A coryneform bacterium in which a DNA coding for a diaminopimelate decarboxylase and a DNA coding for a diaminopimelate dehydrogenase are enhanced is cultivated in a medium to allow L-lysine to be produced and accumulated in a culture, and L-lysine is collected from the culture.
摘要翻译：将编码二氨庚二酸脱羧酶的DNA和编码二氨基庚二酸脱氢酶的DNA增强的棒杆菌在培养基中培养，使得L-赖氨酸在培养物中生成并积累，从培养物中收集L-赖氨酸。

9. 发明专利

DE69736699D1 未知
公开(公告)号：DE69736699D1
公开(公告)日：2006-11-02
申请号：DE69736699
申请日：1997-12-05
申请人： AJINOMOTO KK
发明人： HAYAKAWA ATSUSHI , SUGIMOTO MASAKAZU , YOSHIHARA YASUHIKO , NAKAMATSU TSUYOSHI
IPC分类号： C12N15/09 , C12N15/54 , C07H21/04 , C12N1/21 , C12N9/12 , C12N9/88 , C12N15/00 , C12N15/60 , C12N15/77 , C12P13/08 , C12R1/15
摘要： A recombinant DNA autonomously replicable in cells of coryneform bacteria, comprising a DNA sequence coding for an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and a DNA sequence coding for a diaminopimelate decarboxylase; a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a diaminopimelate decarboxylase; and a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture.

10. 发明专利

DE69929264T2 未知
公开(公告)号：DE69929264T2
公开(公告)日：2006-08-31
申请号：DE69929264
申请日：1999-01-12
申请人： AJINOMOTO KK
发明人： SUGA MIKIKO , SUGIMOTO MASAKAZU , OSUMI TSUYOSHI , NAKAMATSU TSUYOSHI , HIBINO WATARU , ITO MIKA
IPC分类号： C12N15/09 , C12N15/53 , C12N1/20 , C12N1/21 , C12N9/04 , C12P13/06 , C12R1/13 , C12R1/15
摘要： Disclosed is a coryneform bacterium having resistance to azaserine or beta -(2-thienyl)-DL-alanine and having L-serine productivity. Also, disclosed are D-3-phosphoglycerate dehydrogenase derived from a coryneform bacterium, in which feedback inhibition by L-serine is desensitized; the D-3-phosphoglycerate dehydrogenase, which is obtainable from a coryneform bacterium having resistance to azaserine or beta -(2-thienyl)-DL-alanine and having L-serine productivity; the D-3-phosphoglycerate dehydrogenase having an amino acid sequence depicted in SEQ ID NO : 12 in Sequence Listing or the sequence including substitution, addition or deletion of one or more amino acids, wherein an amino acid residue corresponding to the 325th glutamic acid residue of the amino acid sequence in the SEQ ID NO : 12 is replaced with an amino acid other than glutamic acid; the D-3-phosphoglycerate dehydrogenase that has an amino acid sequence depicted in SEQ ID NO : 11 in Sequence Listing; a DNA coding for the D-3-phosphoglycerate dehydrogenase described above; the DNA that has a base sequence depicted in SEQ ID NO : 13 in Sequence Listing; a coryneform bacterium which harbors a recombinant DNA containing the DNA; and a method of producing L-serine, comprising the steps of cultivating the bacterium described above in a medium to allow accumulation of L-serine in the medium, and collecting the L-serine from the medium.

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