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    • 8. 发明公开
    • 남세균 글리코겐 신타제를 이용한 식물체의 광합성과전분함량 증대 방법
    • 通过CYANOBACTICIAL GLYCOGEN SYNTHASE操作高效植物的光合作用和淀粉含量
    • KR1020070079408A
    • 2007-08-07
    • KR1020060010045
    • 2006-02-02
    • 한국생명공학연구원충남대학교산학협력단
    • 김한나정석원김현순전재흥정혁박연일
    • C12N15/05C12N15/09C12N15/10C12N1/12
    • A method for preparing a transformed plant is provided to obtain the transformed plant which is able to express glycogen synthetase and significantly increase the production amount and the size of tuber during in vitro culture, decreases the starch particle size of a potato tuber and has constant size distribution, and be able to increase the potato production amount per area in accordance with the starch concentration increase. The DNA coding glycogen syntase cglgA derived from Synechocystis sp. PCC6803 includes a sequence described as SEQ ID : NO. 1. The method for preparing a transformed plant expressing the cglgA comprises the steps of: (a) transforming plant cells using a polynucleotide sequence having (i) a nucleotide sequence coding the cglgA and described as SEQ ID : NO. 1, (ii) a promoter which is operably linked to the nucleotide sequence(i) and acts on the plant cells to form RNA molecules, and (iii) 3'-non-translation portion which acts on the plant cells to induce polyadenylation of 3'-terminal of the RNA molecules; (b) selecting the transformed plant cells using an Agrobacterium tumefaciens-binary vector system; and (c) after obtaining a redifferentiated shoot from the transformed plant cells, culturing the shoot in a culture medium to obtain a transformed plant.
    • 提供了一种制备转化植物的方法,以获得能够表达糖原合成酶的转化植物,并且在体外培养期间显着增加块茎的产量和大小,降低马铃薯块茎的淀粉粒度并具有恒定的大小 分布,并且能够根据淀粉浓度增加来增加每个面积的马铃薯产量。 来自集胞藻(Synechocystis sp。)的编码糖原合成酶cglgA的DNA PCC6803包括描述为SEQ ID NO: 1.用于制备表达cglgA的转化植物的方法包括以下步骤:(a)使用多核苷酸序列转化植物细胞,所述多核苷酸序列具有(i)编码cglgA并描述为SEQ ID NO: 1,(ii)与核苷酸序列(i)可操作地连接并作用于植物细胞以形成RNA分子的启动子,和(iii)作用于植物细胞诱导多聚腺苷酸化的3'-非翻译部分 RNA分子的3'末端; (b)使用根癌土壤杆菌二元载体系统选择转化的植物细胞; 和(c)在从转化的植物细胞获得再分化的芽后,在培养基中培养芽以获得转化的植物。