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    • 6. 发明授权
    • 식물생장조절물질의 고효율 스크리닝 방법
    • 식물생장조절물질의고효율스크리닝방법
    • KR100463714B1
    • 2004-12-29
    • KR1020020036512
    • 2002-06-27
    • 한국생명공학연구원
    • 곽상수이행순권석윤김선일김창진이향범이상한최선미
    • C12Q1/02
    • G01N33/5097G01N2430/20G01N2500/00
    • The present invention relates to a method for high throughput screening of plant growth regulator, more particularly to the method comprising; 1) culturing phytomixotrophic cells and candidates of plant growth regulator which were added in a microwell plate, 2) treating 2,3,5-triphenyltetrazolium chloride thereto, 3) reacting thereof by adding ethanol after removing solutions from microwells, 4) transferring the reacting solution into the new microwell plate, and 5) measuring optical density with a high throughput screening reader. Since the method of the present invention can rapidly and conveniently screen many samples and can also evaluate in vivo activities of plant growth regulators, it can effectively be used as a screening method for plant growth inhibitors and activators.
    • 本发明涉及用于植物生长调节剂的高通量筛选的方法,更具体地涉及包括: 1)培养加入到微孔板中的植物营养细胞和植物生长调节剂的候选物,2)处理2,3,5-三苯基四唑氯化物,3)从微孔中去除溶液后加入乙醇使其反应,4)将反应 溶液加入到新的微孔板中,以及5)用高通量筛选读数器测量光密度。 由于本发明的方法可以快速方便地筛选许多样品,并且还可以评估植物生长调节剂的体内活性,因此它可以有效地用作植物生长抑制剂和活化剂的筛选方法。
    • 7. 发明公开
    • 류머티스 관절염 치료용 프로디지오신 조성물
    • 用于治疗感染性ARTHRITIS的原料药组合物
    • KR1020020081801A
    • 2002-10-30
    • KR1020010021191
    • 2001-04-19
    • 한국생명공학연구원
    • 김환묵한상배이창우이기훈박세형이상한김형진김영국
    • A61K31/4025
    • A61K31/4025
    • PURPOSE: A composition containing as an effective ingredient prodigiosin separated from Serratia marcescens is provided which can treat initial rheumatic arthritis and progressive rheumatic arthritis without side effects. CONSTITUTION: This prodigiosin composition for treatment of rheumatic arthritis contains prodigiosin of the formula 1 separated from Serratia marcescens B-1231, KCTC 0386BP. The pharmacological mechanism of the prodigiosin to rheumatic arthritis is identified by administration to a DBA/1 mouse as a model animal of collagen-induced rheumatic arthritis either at early onset of or after rheumatic arthritis, measuring a treatment effect and then investigating formation inhibition capacity of cytokine.
    • 目的:提供一种组合物,含有作为有效成分的从沙雷氏菌粘质沙雷菌分离出的雌激素,可以治疗初始风湿性关节炎和进行性风湿性关节炎,无副作用。 构成:用于治疗风湿性关节炎的这种生殖细胞因子组合物含有与粘质沙雷氏菌B-1231,KCTC 0386BP分离的式1的天蛾素。 通过给予DBA / 1小鼠作为胶原诱导的风湿性关节炎的模型动物,在风湿性关节炎的早期或之后,测定治疗效果,然后研究抑制生物体的形成抑制能力,鉴定了生殖细胞因子对风湿性关节炎的药理作用机制 细胞因子。