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    • 5. 发明公开
    • 셀레늄 함유 배지를 이용한 양수 내 태아 유래 중간엽줄기세포의 제조방법
    • 使用含硒介质制造来自生物流体的子宫腔传导性干细胞的方法
    • KR1020130003159A
    • 2013-01-09
    • KR1020110064261
    • 2011-06-30
    • 주식회사 스템메디언스
    • 유승권이중한전은경윤병선문재희이장호이황희이종원조경식
    • C12N5/0775C12N1/38A61K8/98
    • PURPOSE: A cell proliferation promoting agent containing selenium and a method for preparing mesenchymal stem cells derived from a fetus in amniotic fluid are provided to lower the cost for preparing the mesenchymal stem cells and to obtain the mesenchymal stem cells with excellent effect on cell growth and skin regeneration ability. CONSTITUTION: A cell proliferation promoting agent contains selenium as an active ingredient. The cell is a mesenchymal stem cell. A method for preparing the mesenchymal stem cells derived from a fetus in amniotic fluid comprises a step of culturing amniotic fluid-derived cells in a medium containing 5-10% FBS and 1-20 ng/ml of selenium. The medium additionally contains bFGF(basic fibroblast growth factor). A method for preparing the medium for culturing the mesenchymal stem cells comprises: a step of sub-culturing the amniotic fluid-derived cells in the medium containing FBS and selenium and collecting the mesenchymal stem cells; and a step of culturing the mesenchymal stem cells in a serum-free medium for 1-10 days and preparing a conditioned medium.
    • 目的:提供含有硒的细胞增殖促进剂和用于制备源自羊水中胎儿的间充质干细胞的方法,以降低间充质干细胞的制备成本,并获得对细胞生长具有极好作用的间充质干细胞, 皮肤再生能力。 构成:细胞增殖促进剂含有硒作为活性成分。 细胞是间充质干细胞。 用于制备源自羊水的胎儿的间充质干细胞的方法包括在含有5-10%FBS和1-20ng / ml硒的培养基中培养羊水源细胞的步骤。 该培养基另外含有bFGF(碱性成纤维细胞生长因子)。 用于培养间充质干细胞的培养基的方法包括:在含有FBS和硒的培养基中培养来自羊水的细胞并收集间充质干细胞的步骤; 以及在无血清培养基中培养间充质干细胞1-10天并制备条件培养基的步骤。
    • 9. 发明公开
    • 양수 내 태아 유래 중간엽줄기세포를 이용한 성장인자의 대량생산 방법
    • 使用来自生物体液体的衍生的间充质干细胞生长促进因子的大规模生产
    • KR1020100105165A
    • 2010-09-29
    • KR1020090024054
    • 2009-03-20
    • 주식회사 스템메디언스
    • 유승권윤병선전은경문재희김종건정혜연이중한박을순맹이삭김준성이장호이황희이종원조경식
    • C12N5/071C12N5/02
    • C12N5/0605C12N5/0668C12N2501/115
    • PURPOSE: A mesenchymal stem cell derived from fetus in amniotic fluid by culturing is provided to ensure pluripotency and to massively produce growth factors. CONSTITUTION: A mesenchymal stem cell derived from fetus in amniotic fluid is prepared by culturing the fetus-derived cells in a medium containing 10% of FBS(fetal bovaine seru) and 1-20 ng/ml of bFGF(basic fibroblast growth factor). The medium is low glucose DMEM(Dulbecco's Modified Eagle's Medium) medium. The mesoderm-derived cell is adipocyte, osteocyte, or chondrocyte. A method for massively producing the human growth factor from mesenchymal stem cell comprises: a step of isolating fetus-derived cell from a lying-in women; a step of sub-culturing in a medium containing FBS and bFGF to obtain mesenchymal stem cells; and a step of culturing the mesenchymal stem cell in serum free medium for 2-7 days to prepare a conditioned medium.
    • 目的:提供通过培养从羊水中产生的间充质干细胞,以确保多能性并大量产生生长因子。 构成:通过在含有10%FBS(胎牛血清)和1-20ng / ml bFGF(碱性成纤维细胞生长因子)的培养基中培养来自胎儿的细胞来制备源自羊水中的胎儿的间充质干细胞。 培养基为低糖DMEM(Dulbecco's Modified Eagle's Medium)培养基。 中胚层衍生的细胞是脂肪细胞,骨细胞或软骨细胞。 从间充质干细胞大规模生产人生长因子的方法包括:从躺卧妇女中分离胎儿来源的细胞的步骤; 在含有FBS和bFGF的培养基中亚培养以获得间充质干细胞的步骤; 以及在无血清培养基中培养间充质干细胞2-7天以制备条件培养基的步骤。
    • 10. 发明公开
    • 3D-페이스 오아 바디 모델링에 사용자(회원)의 사진이미지를 입혀 인터넷 상으로 불러오는 방법과 이미지를객체로 나누어 그 객체위에 색조화장과 가발, 안경 등을입혀 보는 방법
    • 使用3D脸部或身体建模对客户照片图像进行各种对象的方法
    • KR1020000059236A
    • 2000-10-05
    • KR1020000042548
    • 2000-07-24
    • 조경식
    • 조경식
    • G06Q50/00
    • PURPOSE: A method for combining a customer photo image with various objects is provided to put the photo image on a 3D modelling, send the combined image over the internet, and put coloured makeup, glasses, a wig or other accessaries on the combined image in order to offer an environment similar to a real world. CONSTITUTION: A method for combining a customer photo image with various objects comprises the steps of accessing a site server over the internet at a user personal computer(S100), performing a member subscription process at the site server(S101), sending a photo image of a user face or a body(S102), putting the sent image on a 3D modelling, storing the image in a database by various object types such as an eye, a nose, a neck, a hair and so on, storing URLs of the objects in a data processor(S104), the server calling the user to send a necessary image if a sent photo does not satisfy(S105), the user trying to put various kinds of the commodities on the photo image(S106), selecting a commodity appropriate for the user himself or herself(S107) and purchasing the commodity(S108).
    • 目的:提供将客户照片图像与各种物体相结合的方法,将照片图像放在3D建模上,通过互联网发送组合图像,并将彩色化妆品,眼镜,假发或其他配件放在组合图像中 为了提供类似于现实世界的环境。 构成:将客户照片图像与各种对象组合的方法包括以下步骤:在用户个人计算机上通过因特网访问站点服务器(S100),在站点服务器执行成员订阅处理(S101),发送照片图像 的用户面部或身体(S102),将发送的图像放置在3D建模上,通过眼睛,鼻子,颈部,头发等各种对象类型将图像存储在数据库中,存储URL 数据处理器中的对象(S104),如果发送的照片不满足,则服务器呼叫用户发送必要的图像(S105),尝试将各种商品放在照片图像上的用户(S106),选择 适合用户自己的商品(S107)和购买商品(S108)。