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1. 发明公开

KR1020070112553A 천연 식물 추출물을 이용한 수생곰팡이 억제제 失效
标题翻译：包括天然植物提取物的抗真菌药物
公开(公告)号：KR1020070112553A
公开(公告)日：2007-11-27
申请号：KR1020060045588
申请日：2006-05-22
申请人： 주식회사 바이넥스
发明人： 정창화 , 전경동 , 양문휴 , 김원석 , 안경출 , 이백천
IPC分类号： A61K36/888 , A61P31/10
CPC分类号： A61K36/882
摘要： An agent for inhibiting fungal diseases of fish is provided to inhibit the growth of fungi, selectively control and prevent the fungi and replace chemotherapeutic agents and antibiotics such as Malachite green, thereby being used as an environmental-friendly natural therapeutic agent. A therapeutic composition for fungal diseases of fish having anti-fungal activity on Saprolegnia parasitica, the fungal diseases-causing bacteria of fish, comprises an extract of sweet flag as an effective ingredient, wherein the extract is prepared by using a solvent such as water, hydrophilic organic solvent or a mixture solvent thereof.
摘要翻译：提供了抑制鱼类真菌病的药剂，以抑制真菌的生长，选择性地控制和预防真菌，替代化学治疗剂和抗生素如孔雀石绿，从而被用作环保型天然治疗剂。一种用于真菌性真菌活性的鱼的真菌性疾病的治疗组合物，所述鱼类的真菌性疾病引起的细菌为珊瑚菌，其中包括甜味提取物作为有效成分，其中所述提取物是通过使用溶剂如水，亲水性有机溶剂或其混合溶剂。

2. 发明授权

KR100765357B1 고스트 비브리오 앵귈라룸 및 이를 함유하는 비브리오증의예방용 백신 失效
标题翻译： GHOST VIBRIO ANGUILLARUM和VACCINE用于预防含有其的VIBRIOSIS
公开(公告)号：KR100765357B1
公开(公告)日：2007-10-10
申请号：KR1020060099244
申请日：2006-10-12
申请人： 부경대학교 산학협력단 , 주식회사 바이넥스
发明人： 김기홍 , 권세련 , 남윤권 , 김성구 , 정창화 , 박소진 , 김원석 , 이백천
IPC分类号： C12N15/63 , C12N1/21
摘要： A microorganism ghost Vibrio anguillarum and a vaccine for preventing vibriosis containing the same microorganism are provided to supply a new vibriosis-preventing vaccine having surface antigenicity similar to live Vibrio anguillarum and no pathogenicity without treatment with toxic chemicals such as formalin, so that productivity of cultured fishes is improved. A microorganism ghost Vibrio anguillarum PRKG(KFCC 11377P) is prepared by (a) transforming Vibrio anguillarum with a recombinant vector pRK-lambdaPR-cI-Elysis containing lambdaPR promoter/cI repressor and lysis E gene and culturing the transformed Vibrio anguillarum at 25-30 deg.C, (b) increasing culture temperature to 40-43 deg.C when cell density(OD600) of culture medium reaches at 0.2-0.25 and culturing the microorganism, and (c) obtaining the transformed Vibrio anguillarum when cell density reduction of culture medium is stopped.
摘要翻译：提供微生物幽灵弧菌弧菌和用于预防含有相同微生物的弧菌病的疫苗，以提供具有类似于活鳗弧菌的表面抗原性的新型弧菌预防疫苗，并且不用有毒的化学物质如福尔马林处理而无致病性，从而培养鱼改善了。通过（a）用含有λPR启动子/ cI阻遏物和裂解E基因的重组载体pRK-λPR-cI-Elysis转化鳗弧菌来制备微生物幽门弧菌PRKG（KFCC 11377P），并在25-30℃下培养转化的鳗弧菌（b）当培养基的细胞密度（OD600）达到0.2-0.25时，培养温度升至40-43℃，培养微生物，（c）当细胞密度降低时获得转化的鳗弧菌培养基停止。

3. 发明公开

KR1020090067489A 비브리오증 유발 세균에 대한 천연항균제 无效
标题翻译：抗生素的自然抗生素
公开(公告)号：KR1020090067489A
公开(公告)日：2009-06-25
申请号：KR1020070135166
申请日：2007-12-21
申请人： 주식회사 바이넥스
发明人： 정창화 , 김윤임 , 김성미
IPC分类号： A01N65/24 , A01N65/22
CPC分类号： A01N65/24 , A01K61/13 , A01N65/22 , A01N65/28 , A01N65/36 , A01N2300/00 , A61K36/53 , A61K36/61 , A61K36/75
摘要： A natural antibacterial agent against a bacteria which causes vibriosis is provided to have excellent antibacterial activity and suppress antibacterial agent resistant bacteria. A natural antibacterial agent to a bacteria which causes vibriosis comprises cinnamon leaf extract, Bergamot extract, lavender french extract, or clove bud extract as an active ingredient. A method for producing the natural antibacterial agent comprises: a step of heating water over 100°C in a plant extraction evaporator and generating steam; a step of reacting steam with pulverized natural plant to extract essential oil; and a step of passing the mixture of stream and essential oil through cooler and isolating steam and essential oil to obtain pure essential oil.
摘要翻译：本发明提供了抗菌活性高的抗细菌抗菌药物。引起弧菌病的细菌的天然抗菌剂包括肉桂叶提取物，香柠檬提取物，薰衣草法国提取物或丁香芽提取物作为活性成分。一种生产天然抗菌剂的方法包括：在植物提取蒸发器中加热100℃以上的水并产生蒸汽的步骤; 将蒸汽与粉碎的天然植物反应以提取精油的步骤; 以及通过冷却器和精油的混合物使蒸汽和精油分离以获得纯精油的步骤。

4. 发明公开

KR1020080037457A 바실러스 폴리퍼멘티쿠스, 바실러스 리케니포르미스 및사카로마이세스 세르비지에를 포함하는 어류사료첨가용조성물 失效
标题翻译：用于饲喂含有巴豆酵母聚合酶，巴氏酵母菌和细菌菌丝体的鱼的组合物
公开(公告)号：KR1020080037457A
公开(公告)日：2008-04-30
申请号：KR1020060104653
申请日：2006-10-26
申请人： 주식회사 바이넥스
发明人： 정창화 , 전경동 , 김원석 , 이백천
IPC分类号： A23K1/18 , A23K1/16 , A23K1/17 , A23K1/00
CPC分类号： A23K20/195 , A23K50/80
摘要： A fish feed additive composition contains Bacillus polyfermenticus, Bacillus licheniformis and Saccharomyces cerevisiae is provided to increase the growth and nonspecific immunoreactivity of fish and feed efficiency when added to fish feed. A fish feed additive composition contains at least one of Bacillus polyfermenticus, Bacillus licheniformis and Saccharomyces cerevisiae. The Bacillus polyfermenticus, Bacillus licheniformis and Saccharomyces cerevisiae contain 1x10^8 to 10^10 CFU/kg, 1x10^8 to 10^10 CFU/kg and 1x10^7 to 10^9 CFU/kg respectively. The composition is added to feed for olive founder and Sebastes schlegeli.
摘要翻译：鱼饲料添加剂组合物含有多发性芽孢杆菌（Bacillus polyfermenticus），地衣芽孢杆菌（Bacillus licheniformis）和酿酒酵母（Saccharomyces cerevisiae），以增加鱼类的生长和非特异性免疫反应性，并加入鱼饲料时的饲料效率。鱼饲料添加剂组合物含有多发芽孢杆菌，地衣芽孢杆菌和酿酒酵母中的至少一种。芽孢杆菌（Bacillus polyfermenticus），地衣芽孢杆菌（Bacillus licheniformis）和酿酒酵母（Saccharomyces cerevisiae）分别含有1×10 8至10 10 CFU / kg，1×10 8至10 10 CFU / kg和1×10 7至10 9 CFU / kg。该组合物添加到橄榄创始人和Sebastes schlegeli的饲料中。

5. 发明授权

KR101006556B1 어류 세균성 병원균에 대한 항균용 조성물 失效
标题翻译：针对链球菌的抗菌组合物
公开(公告)号：KR101006556B1
公开(公告)日：2011-01-07
申请号：KR1020070134699
申请日：2007-12-20
申请人： 주식회사 바이넥스
发明人： 정창화 , 김선정 , 김윤임
IPC分类号： A01N65/20 , A01N65/22 , A01N65/08
摘要： 본 발명은 고삼 추출물, 관중 추출물 및 타임 추출물로 이루어진 군으로부터 선택된 1종 이상의 식물 추출물을 포함하는 항균용 조성물에 관한 것이다.
본 발명에 따르면, 상기 항균용 조성물을 이용하여 우리나라의 담수 및 해수 양식에 막대한 경제적 손실을 입히고 있는 세균성 질병 원인 중 하나인 스트렙토균의 어류에 대한 감염을 예방 및 치료 할 수 있다. 또한 한약재로 널리 사용되는 고삼, 관중 추출물과 허브 식물인 타임정유 추출물을 이용하여 궁극적으로는 장기간에 걸친 사용으로 내성균의 출현이 우려되는 항생제를 대체할 수 있으며, 기존 항생제와 동등 또는 그 이상의 효과를 가지면서 환경 친화적이고, 부가가치가 높은 천연항균제를 제공할 수 있다.
항생제, 항균제, 연쇄구균, 고삼(Sophora flavescens), 관중(Dryopteris crassirhizoma), 타임(Thymus vulgaris)

6. 发明公开

KR1020090066949A 어류 세균성 병원균에 대한 항균용 조성물 失效
标题翻译：抗菌素组合物
公开(公告)号：KR1020090066949A
公开(公告)日：2009-06-24
申请号：KR1020070134699
申请日：2007-12-20
申请人： 주식회사 바이넥스
发明人： 정창화 , 김선정 , 김윤임
IPC分类号： A01N65/20 , A01N65/22 , A01N65/08
CPC分类号： A01N65/20 , A01K61/13 , A01N65/04 , A01N65/22 , A01N2300/00 , A61K36/11 , A61K36/48 , A61K36/53
摘要： A composition for antibacterial, which contains plant extract is provided to prevent and treat the infection to fishes of streptococcus sp. and obtain eco-friendly and natural antibacterial agent. An antibacterial composition against bacterial pathogen of fishes comprises a plant extract containing Sophora flavescens extract, Dryopteris crassirhizoma extract, and Thymus vulgaris extract. The bacterial pathogen is Streptococcus sp. The plant extract is isolated using water, ethanol, isopropyl alcohol, methanol, propanol, butanol, glycerine, butylene glycol or their mixture. The extracts of Thymus vulgaris and Sophora flavescens is mixed in a weight ratio of 1:0.2-1:5. The extracts of Thymus vulgaris and Dryopteris crassirhizoma is mixed in a weight ratio of 1:0.2-1:5.
摘要翻译：提供含有植物提取物的抗菌组合物，用于预防和治疗链球菌鱼的感染。并获得环保和天然抗菌剂。针对鱼类细菌病原体的抗菌组合物包含含有苦参皂甙提取物，，，提取物和寻常aris草提取物的植物提取物。细菌病原体是链球菌属使用水，乙醇，异丙醇，甲醇，丙醇，丁醇，甘油，丁二醇或其混合物分离植物提取物。将寻常百里香和苦参的提取物以1:0.2-1:5的重量比混合。将寻常Th草和Dry The草的提取物以1：0.2-1：5的重量比混合。

7. 发明公开

KR1020070049382A 천연식물 추출물을 이용한 어류 기생충 구충용 조성물 失效
标题翻译：使用天然植物提取物的鱼类寄生虫的安全性组合物
公开(公告)号：KR1020070049382A
公开(公告)日：2007-05-11
申请号：KR1020050106489
申请日：2005-11-08
申请人： 주식회사 바이넥스
发明人： 정창화 , 김원석 , 이백천
IPC分类号： A61K36/58 , A61K36/53 , A61K36/899
CPC分类号： Y02A40/81 , Y02A40/814 , A61K36/58 , A61K36/53 , A61K36/899
摘要： PROBLEM TO BE SOLVED: To develop a fish parasite-controlling substance from a vegetable natural substance as a raw material to control parasitic diseases occurred on culture of fishes, in more detail, to provide a method for applying an extract of Azadirachta indica, Thymus vulgaris or Cymbopogon citrates to an aquaculture industry to use the extract as a parasitic infection medicine. SOLUTION: This fish parasite-controlling composition can solve the limits and side effects of fish parasite control, occurred on the employments of conventional chemical preparations having been used for fish parasite infection diseases, do not affect cultured fishes themselves, has excellent effects for controlling fish parasites, and simultaneously promotes the physiological activities of fishes to quickly recover the fishes from their diseases. COPYRIGHT: (C)2007,JPO&INPIT

8. 发明公开

KR1020120104803A 가자, 오배자 및 레몬그라스 추출물을 함유하는 어류 병원성 세균에 대한 항균용 조성물 无效
标题翻译：抗真菌组合物，包含真菌病原菌细菌，其中包括终末胃肠外注射液，GALLA RHOIS和LEMONGRASS
公开(公告)号：KR1020120104803A
公开(公告)日：2012-09-24
申请号：KR1020110022427
申请日：2011-03-14
申请人： 주식회사 바이넥스
发明人： 정창화 , 김무상 , 김성미
IPC分类号： A61K36/185 , A61K36/22 , A61K36/752 , A61P31/04
摘要： PURPOSE: An antibacterial composition containing Terminalia chebula Retzius, Galla Rhois, or Cymbopogon citratus extract is provided to prevent and treat infection in fishes and to replace antibiotics. CONSTITUTION: An antibacterial composition contains Terminalia chebula Retzius, Galla Rhois, or Cymbopogon citratus extract as an active ingredient. The composition has an antibacterial activity against Vibrio anguillarum, Edwardsiella tarda, and Streptococcus iniae. A method for preparing the composition comprises: a step of extracting Terminalia chebula Retzius or Galla Rhois using organic solvent; a step of extracting Cymbopogon citrates by steam distillation; and a step of mixing the extract or mixture extract with veterinarily acceptable excipient and diluents.
摘要翻译：目的：提供含有Terminalia chebula Retzius，Galla Rhois或Cymbopogon citratus提取物的抗菌组合物，用于预防和治疗鱼类感染和替代抗生素。构成：抗菌组合物含有Terminalia chebula Retzius，Galla Rhois或Cymbopogon citratus提取物作为活性成分。该组合物对鳗弧菌，Edwardsiella tarda和链球菌有抗菌活性。一种制备该组合物的方法，包括：使用有机溶剂萃取终浓度悬铃木Retzius或Galla Rhois的步骤; 通过蒸汽蒸馏提取柠檬酸Cymbopogon的一个步骤; 以及将提取物或混合物提取物与兽医学可接受的赋形剂和稀释剂混合的步骤。

9. 发明授权

KR100857771B1 바실러스 폴리퍼멘티쿠스, 바실러스 리케니포르미스 및사카로마이세스 세르비지에를 포함하는 어류사료첨가용조성물 失效
标题翻译：用于添加饲料的组合物，其包含芽孢杆菌多发性筋膜炎（Bacillus polyfermenticus），地衣芽孢杆菌（Bacillus licheniformis）和酿酒酵母（Saccharomyces serevisiae）
公开(公告)号：KR100857771B1
公开(公告)日：2008-09-09
申请号：KR1020060104653
申请日：2006-10-26
申请人： 주식회사 바이넥스
发明人： 정창화 , 전경동 , 김원석 , 이백천
IPC分类号： A23K1/18 , A23K1/16 , A23K1/17 , A23K1/00
摘要： 본 발명은 바실러스 폴리퍼멘티쿠스 (
Bacillus

polyfermenticus ), 바실러스 리케니포르미스 (
Bacillus

licheniformis ) 및 사카로마이세스 세르비지에 (
Saccharomyces
cerevisiae ) 중 어느 하나이상을 포함하는 것을 특징으로 하는 어류사료 첨가용 조성물에 관한 것이다.
본 발명에 따르면, 상기 세 균주가 단독 또는 혼합되어 있는 사료첨가제는 어류의 사료에 첨가하여 어류의 성장, 사료효율 및 비특이적 면역반응을 증가시킬 수 있다. 특히, 세 균주가 혼합 조성된 사료첨가제의 경우는 각 균주들이 상호보완적인 작용을 나타냄으로써, 더욱 효과적인 사료첨가제로서 사용이 기대된다.
바실러스 폴리퍼멘티쿠스, 바실러스 리케니포르미스, 사카로마이세스 세르비지에, 사료첨가제, 사료효율, 비특이적 면역반응

10. 发明授权

KR100801143B1 바실러스 폴리퍼멘티쿠스와 사카로마이세스 세르비지에의혼합배양 방법 失效
标题翻译：酿酒酵母混合物和沙门氏菌混合物的方法
公开(公告)号：KR100801143B1
公开(公告)日：2008-02-05
申请号：KR1020060077872
申请日：2006-08-17
申请人： 주식회사 바이넥스
发明人： 정창화 , 전경동 , 김원석 , 안경출 , 이백천
IPC分类号： C12N1/20 , C12N1/00
摘要： A method for mixed-culturing Bacillus polyfermenticus and Saccharomyces cerevisiae is provided to be able to produce a feed additive showing excellent effects such as mortality decrease and body weight increase due to interaction between the Bacillus polyfermenticus and Saccharomyces cerevisiae. A method for mixed-culturing Bacillus polyfermenticus and Saccharomyces cerevisiae comprises the steps of: (a) inoculating Saccharomyces cerevisiae into a producing culture medium and then culturing it; and (b) inoculating Bacillus polyfermenticus into the producing culture medium after 9-15 hours are passed from the step(a) and then culturing it at a temperature of 30-37 deg.C under the pH of 6.0-8.0 with the air flow amount of 0.5-1.0 vvm. A culture medium composition for mixed-culturing Bacillus polyfermenticus and Saccharomyces cerevisiae comprises an ingredient consisting of 1-13 wt.% of glucose, 1-14 wt.% of corn starch, 1-3 wt.% of bacto-tryptone, and 1-3 wt.% of an yeast extract; and 0.05-0.3 wt.% of calcium chloride dihydrate, 0.05-0.5 wt.% of ammonium sulfate, 0.5-3 wt.% of potassium dihydrogen phosphate, 0.5-3 wt.% of potassium phosphate dibasic; 0.001-0.01 wt.% of FeSO4.7H2O, and 0.001-0.01 wt.% of ZnSO4.7H2O as trace ingredients.
摘要翻译：提供了用于混合培养芽孢杆菌多糖和酿酒酵母的方法，以能够产生显示出优异效果的饲料添加剂，例如由于芽孢杆菌（Bacillus polyfermenticus）和酿酒酵母（Saccharomyces cerevisiae）之间的相互作用导致的死亡率降低和体重增加。混合培养芽孢杆菌多糖和酿酒酵母的方法包括以下步骤：（a）将酿酒酵母接种到产生培养基中，然后培养; 和（b）从步骤（a）经过9-15小时后将芽孢杆菌接种到生产培养基中，然后在温度为30-37℃，pH 6.0-8.0的条件下，用空气流用量为0.5-1.0vvm。用于混合培养芽孢杆菌多糖和酿酒酵母的培养基组合物包含由1-13重量％的葡萄糖，1-14重量％的玉米淀粉，1-3重量％的细菌胰蛋白胨和1 -3重量％的酵母提取物; 和0.05-0.3重量％的氯化钙二水合物，0.05-0.5重量％的硫酸铵，0.5-3重量％的磷酸二氢钾，0.5-3重量％的磷酸氢二钾; 0.001-0.01重量％的FeSO 4·7H 2 O和0.001-0.01重量％的ZnSO 4·7H 2 O作为痕量成分。

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