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1. 发明授权

KR100390385B1 미생물의 동결건조방법 失效
标题翻译： 미생물의동결건조방법
公开(公告)号：KR100390385B1
公开(公告)日：2003-07-07
申请号：KR1020000037709
申请日：2000-07-03
申请人： 장덕진 , 김상종
发明人： 장덕진 , 김상종 , 이규호 , 이동훈 , 조장천 , 박지은
IPC分类号： C12N1/04
摘要： PURPOSE: A freeze-drying method of a microorganism is provided, thereby maintaining the survival rate of luminescent microorganism up to 50% or more and the rate of luminescence up to 45%. CONSTITUTION: The freeze-drying method of a microorganism is accomplished by adding 0.01M or more trehalose into a growth medium; inoculating a microorganism into the trehalose-containing growth medium; cultivating the microorganism with shacking; and freeze-drying the cultured medium, in which the microorganism is a luminescent microorganism; the cultivation is carried out for 1 to 30 minutes; and the trehalose is added to the cell during freeze-drying process.
摘要翻译：目的：提供一种微生物的冷冻干燥方法，由此将发光微生物的存活率保持在50％以上，发光率维持在45％以下。组成：微生物的冷冻干燥方法通过将0.01M或更多的海藻糖加入生长培养基中完成; 将微生物接种到含海藻糖的生长培养基中; 摇晃培养微生物; 并冷冻干燥其中微生物是发光微生物的培养基; 培养进行1至30分钟; 并在冷冻干燥过程中将海藻糖加入到细胞中。

2. 发明公开

KR1020020004146A 미생물의 동결건조방법 失效
标题翻译：微生物冷冻干燥方法
公开(公告)号：KR1020020004146A
公开(公告)日：2002-01-16
申请号：KR1020000037709
申请日：2000-07-03
申请人： 장덕진 , 김상종
发明人： 장덕진 , 김상종 , 이규호 , 이동훈 , 조장천 , 박지은
IPC分类号： C12N1/04
摘要： PURPOSE: A freeze-drying method of a microorganism is provided, thereby maintaining the survival rate of luminescent microorganism up to 50% or more and the rate of luminescence up to 45%. CONSTITUTION: The freeze-drying method of a microorganism is accomplished by adding 0.01M or more trehalose into a growth medium; inoculating a microorganism into the trehalose-containing growth medium; cultivating the microorganism with shacking; and freeze-drying the cultured medium, in which the microorganism is a luminescent microorganism; the cultivation is carried out for 1 to 30 minutes; and the trehalose is added to the cell during freeze-drying process.
摘要翻译：目的：提供微生物的冷冻干燥方法，从而将发光微生物的存活率保持在50％以上，发光率高达45％。构成：微生物的冷冻干燥方法是通过向生长培养基中加入0.01M以上的海藻糖而实现的; 将微生物接种到含海藻糖的生长培养基中; 用棚架培养微生物; 并冷冻干燥培养的培养基，其中微生物是发光微生物; 培养1〜30分钟; 并且在冷冻干燥过程中将海藻糖加入到细胞中。

3. 发明公开

KR1020020007039A 수질 오염 원격 조기 경보 시스템 및 그 방법 无效
标题翻译：早期电力监测水污染的系统和方法
公开(公告)号：KR1020020007039A
公开(公告)日：2002-01-26
申请号：KR1020000040711
申请日：2000-07-14
申请人： 이동훈 , 김상종
发明人： 이동훈 , 김상종 , 이규호 , 장덕진 , 조장천
IPC分类号： G06Q50/00
摘要： PURPOSE: A tele-monitoring system and a method thereof are provided to process water pollution data in the central part at real time by securing a stabilized communication circuit at a low cost and constructing an unmanned data processing system. CONSTITUTION: The system comprises a water contamination measuring device(110) using a luminescence bacteria, an on-the-spot water contamination data analyzing, transmitting and receiving unit(120) loaded in a computer on the spot, a local data processing device(130), a CPU(Central Processing Unit)(140). The on-the-spot water contamination data analyzing and transmitting/receiving unit analyzes the measuring result by a pre-inputted warning standard, processes the result, generates a warming, transmits the warning to a local server via an air communication network and a modem, and notices the warning to a cellular phone of a manager The local data processing device processes the water contamination data by receiving the signals transmitted from the spots, stores the local data in a database, and informs the data to a central processing unit. The CPU generally processes the data informed by many local data processing units.
摘要翻译：目的：提供远程监控系统及其方法，通过以低成本确保稳定的通信电路，构建无人数据处理系统，实时处理中心部分的水污染数据。构成：该系统包括使用发光细菌的水污染测量装置（110），现场装载在计算机中的现场水污染数据分析，发送和接收单元（120），本地数据处理装置 130），CPU（中央处理单元）（140）。现场水污染数据分析和发送/接收单元通过预先输入的警告标准对测量结果进行分析，处理结果，生成升温，通过空中通信网络和调制解调器向本地服务器发送警告并注意到对管理员的蜂窝电话的警告本地数据处理设备通过接收从点发送的信号来处理水污染数据，将本地数据存储在数据库中，并将数据通知给中央处理单元。 CPU通常处理由许多本地数据处理单元通知的数据。

4. 发明公开

KR1020010107164A 폴리하이드록시알카노에이트를 생산하는 미생물 失效
标题翻译：生产微生物的聚羟基烷酸（PHA）和生产PHA的方法
公开(公告)号：KR1020010107164A
公开(公告)日：2001-12-07
申请号：KR1020000028422
申请日：2000-05-25
申请人： 김상종
发明人： 조영철 , 조장천 , 김상종
IPC分类号： C12N1/20
摘要： 본 발명은 폴리하이드록시알카노에이트(PHA : polyhydroxyalkanoate)를 생산하는 미생물에 관한 것으로, 퇴적토에서 분리한
Erwinia chrysanthemi DS-10(KCTC
0783BP)는 글루코스를 기질로 하여 배양하였을 때 총 건조 중량의 80 %이상의 PHA를 생산하여 생분해성 플라스틱을 효율적으로 생산할 수 있다.

5. 发明公开

KR1020010086843A 독성물질 감지용 발광균주 失效
标题翻译：用于检测有毒物质的荧光微生物
公开(公告)号：KR1020010086843A
公开(公告)日：2001-09-15
申请号：KR1020000010763
申请日：2000-03-03
申请人： 이규호 , 김상종
发明人： 이규호 , 박경제 , 김상종 , 이동훈 , 장덕진 , 조장천
IPC分类号： C12N1/20
CPC分类号： C12R1/01 , C12Q1/02 , G01N2520/00
摘要： PURPOSE: Provided is a novel microorganism strain which has detectability for very low level of toxic substances. And a method of detecting the toxic substances using the same and a kit therefor are provided. CONSTITUTION: The novel microorganism strain, YH9-RC (KCTC 0730BP), changes the luminous level of light by reacting with toxic substances. The method of detecting the toxic substances is characterized by using aldehyde substrate to give luminescence to the microorganism. The aldehyde level used in the detection method is 0.01 to 0.015 weight. The toxic substances are selected from the group consisting of phenol, Al, As, Cd, Co, Cr(VI), Fe, Hg, Mn, Se, Zn and their mixture. The microorganism is prepared by the steps of: isolating gram negative strains from clean underground water on R2A agar plate; selecting Janthinobacterium lividium YH9 having highest sensitivity against phenol; and then introducing a luminous gene into the YH9 strain. Plasmid DNA comprising lux gene(pUTluxAB) is used to introduce the luminous gene into the YH9 strain, and the plasmid DNA is introduced into a receptor cell via a triparental conjugation.
摘要翻译：目的：提供一种新的微生物菌株，其具有非常低水平有毒物质的可检测性。并提供了使用该有毒物质检测有毒物质的方法及其试剂盒。构成：新型微生物菌株YH9-RC（KCTC 0730BP）通过与有毒物质反应来改变发光水平。检测有毒物质的方法的特征在于使用醛底物给微生物发光。检测方法中使用的醛水平为0.01〜0.015重量。有毒物质选自苯酚，Al，As，Cd，Co，Cr（VI），Fe，Hg，Mn，Se，Zn及其混合物。通过以下步骤制备微生物：在R2A琼脂平板上从干净的地下水中分离革兰氏阴性菌株; 选择对苯酚具有最高敏感性的黄曲霉YH9; 然后将发光基因引入YH9菌株。使用包含lux基因（pUTluxAB）的质粒DNA将发光基因引入YH9菌株，并通过三亲共轭将质粒DNA引入受体细胞。

6. 发明授权

KR100353390B1 폴리하이드록시알카노에이트를 생산하는 미생물 失效
标题翻译：生产聚羟基链烷酸酯的微生物
公开(公告)号：KR100353390B1
公开(公告)日：2002-09-18
申请号：KR1020000028422
申请日：2000-05-25
申请人： 김상종
发明人： 조영철 , 조장천 , 김상종
IPC分类号： C12N1/20
摘要： 본 발명은 폴리하이드록시알카노에이트(PHA : polyhydroxyalkanoate)를 생산하는 미생물에 관한 것으로, 퇴적토에서 분리한
Erwinia chrysanthemi DS-10(KCTC
0783BP)는 글루코스를 기질로 하여 배양하였을 때 총 건조 중량의 80 %이상의 PHA를 생산하여 생분해성 플라스틱을 효율적으로 생산할 수 있다.

7. 发明授权

KR100353389B1 다핵방향족 탄화수소를 분해하는 균주 失效
标题翻译：分解多核芳香烃的菌株
公开(公告)号：KR100353389B1
公开(公告)日：2002-09-18
申请号：KR1020000028421
申请日：2000-05-25
申请人： 김상종
发明人： 조재창 , 조장천 , 김상종
IPC分类号： C12N1/20
摘要： 본 발명은 토양에서 분리한 신규의
Arthrobacter oxydans JS-15 균주에 관한 것으로, 다핵방향족 탄화수소(PHA : polyaromatic hydrocarbon)를 분해하여 환경오염 정화에 효과적으로 사용할 수 있는 생물 정화체
Arthrobacter oxydans JS-15를 제공한다.

8. 发明公开

KR1020010107163A 다핵방향족 탄화수소를 분해하는 균주 失效
标题翻译：聚氨酯分解剂AR-ROBACTER OXYDANS JS-15
公开(公告)号：KR1020010107163A
公开(公告)日：2001-12-07
申请号：KR1020000028421
申请日：2000-05-25
申请人： 김상종
发明人： 조재창 , 조장천 , 김상종
IPC分类号： C12N1/20
摘要： 본 발명은 토양에서 분리한 신규의
Arthrobacter oxydans JS-15 균주에 관한 것으로, 다핵방향족 탄화수소(PHA : polyaromatic hydrocarbon)를 분해하여 환경오염 정화에 효과적으로 사용할 수 있는 생물 정화체
Arthrobacter oxydans JS-15를 제공한다.

9. 发明授权

KR100357756B1 독성물질 감지용 발광균주 失效
标题翻译：用于检测有毒物质的发光菌株
公开(公告)号：KR100357756B1
公开(公告)日：2002-10-18
申请号：KR1020000010763
申请日：2000-03-03
申请人： 이규호 , 김상종
发明人： 이규호 , 박경제 , 김상종 , 이동훈 , 장덕진 , 조장천
IPC分类号： C12N1/20
摘要： 본 발명은 독성물질 감지용 발광균주에 관한 것으로, 보다 상세하게는 독성물질과 접촉시 발광정도의 변화를 나타내는 미생물인 잔티노박테리움 리비둠 YH9-RC과 상기 미생물의 발광정도의 변화를 이용하여 독성물질을 검사하는 방법 및 독성물질을 검사하는 기기에 관한 것이다. 본원 발명의 발광균주인 잔티노박테리움 리비둠 YH9-RC을 사용할 경우 매우 낮은 농도의 독성물질에 대하여도 감지가 가능하다.

10. 发明公开

KR1020020000280A 환경수에서 바이러스 오염 검출방법 无效
标题翻译：环境污染病毒感染的检测方法
公开(公告)号：KR1020020000280A
公开(公告)日：2002-01-05
申请号：KR1020000034564
申请日：2000-06-22
申请人： 김상종
发明人： 김상종 , 조장천 , 조홍백 , 이승훈
IPC分类号： C12Q1/68
摘要： PURPOSE: Provided is a detection method of Adenovirus and Enteric virus contamination from environment water by multiplex nested polymerase chain reaction PCR) analysis. This detection method can be applied to the detection of other generalized virus. CONSTITUTION: The detection method comprises: making two pairs of Adenovirus primers of 75nM-130nM, which are AV1, AV2, AV3 and AV4, containing SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6 and SEQ ID NO: 7 respectively and three enteric virus primer of 200nM-300nM, which are EV1, EV2 and EV3, containing SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3; carrying on the first PCR by a) substituting enteric virus RNA to DNA by reverse transcription PCR between enteric virus primer EV2 and reverse transcriptase, b) adding Adenovirus primer AV1/AV2 and enteric virus primer EV1 to the above reverse transcription PCR reactants; performing the second PCR by adding Adenovirus primer AV3/AV4 and enteric virus primer EV3; and identifying the expanded virus DNA on the agarose gel. The both PCR consist of mutation step at 94deg.C for 4 minutes, expansion step with repeating the cycles of 30 second at 95deg.C, 30 second at 55deg.C and one minute at 72deg.C for 35 times, and final expansion step for 7 minutes at 72deg.C.
摘要翻译：目的：提供通过多重巢式聚合酶链反应PCR分析环境水中腺病毒和肠道病毒污染的检测方法。该检测方法可应用于其他广义病毒的检测。构成：检测方法包括：将两对75nM-130nM的腺病毒引物分别为含有SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：6和SEQ ID NO的AV1，AV2，AV3和AV4 7个，分别为200nM-300nM的三个肠道病毒引物，其为包含SEQ ID NO：1，SEQ ID NO：2和SEQ ID NO：3的EV1，EV2和EV3; 进行第一次PCR，a）通过肠病毒引物EV2和逆转录酶之间的逆转录PCR将肠病毒RNA取代为DNA，b）向上述逆转录PCR反应物中加入腺病毒引物AV1 / AV2和肠毒素引物EV1; 通过添加腺病毒引物AV3 / AV4和肠道病毒引物EV3进行第二次PCR; 并在琼脂糖凝胶上鉴定扩增的病毒DNA。两个PCR均由94℃的突变步骤4分钟，膨胀步骤在95℃下重复30秒的循环，在55℃下重复30秒，在72℃重复1分钟35次，最后的扩展步骤在72℃下7分钟。

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