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    • 1. 发明公开
    • 올리고뉴클레오티드칩을 이용한 생태계 기능군집 미생물의분석방법, 올리고뉴클레오티드칩 및 올리고뉴클레오티드의염기서열
    • 使用寡核苷酸芯片测定生态系统中微生物功能群体的方法
    • KR1020030094519A
    • 2003-12-18
    • KR1020020027659
    • 2002-05-20
    • 제노프라 주식회사김성천이동훈이규호장덕진
    • 김성천이동훈장덕진이규호박정재김성민박경제임재규이현경김혜령안난희
    • C12Q1/68G06F19/20
    • PURPOSE: A method for assaying functional population of microorganisms in the ecosystem using an oligonucleotide chip are provided, thereby simply and accurately assaying the expression pattern of the functional gene of a specific microorganism, and analysing and removing pollution sources in the ecosystem. CONSTITUTION: A method for assaying functional population of microorganisms in the ecosystem using an oligonucleotide chip comprises the steps of: preparing oligonucleotides having the nucleotide sequences which complementary bind with substance degrading enzyme genes in microorganisms as capture probes; aligning and fixing the oligonucleotides on a slide to prepare the oligonucleotide chip; collecting a sample from the ecosystem, labeling RNA in microorganisms contained in the sample to prepare a capture probe solution, and treating the oligonucleotide chip with a mixture of the capture probe solution and a hybridization solution; and washing, centrifuging and drying the hybridized oligonucleotide chip, and detecting the labeled RNA, wherein the substance is pollution substances containing organic matters and heavy metals.
    • 目的:提供使用寡核苷酸芯片测定生态系统功能性群体的方法,从而简单而准确地测定特定微生物的功能基因的表达模式,分析和消除生态系统中的污染源。 构成:使用寡核苷酸芯片测定生态系统中微生物功能群体的方法包括以下步骤:制备具有与微生物中的物质降解酶基因互补结合的核苷酸序列作为捕获探针的寡核苷酸; 在载玻片上对准和固定寡核苷酸以制备寡核苷酸芯片; 从生态系统收集样品,在样品中包含的微生物中标记RNA以制备捕获探针溶液,并用捕获探针溶液和杂交溶液的混合物处理寡核苷酸芯片; 洗涤,离心和干燥杂交的寡核苷酸芯片,并检测标记的RNA,其中该物质是含有机物质和重金属的污染物质。
    • 2. 发明公开
    • 전기 구이기
    • 电动滚筒
    • KR1020030078383A
    • 2003-10-08
    • KR1020020017403
    • 2002-03-29
    • 장덕진길기순양상용
    • 장덕진양상용길기순
    • A47J37/06
    • PURPOSE: An electric roaster is provided to prevent food from being scorched by easily controlling the temperature of an electric heater, and to remove a bad smell from exhaust air by installing a deodorizing unit. CONSTITUTION: An electric roaster(1) is composed of a plurality of grills(110), a plurality of grill mount units(120), a heater(200), a power control unit for controlling the heater, a plurality of roasting spaces having the heater within, a body(500) including a door(530) for opening/closing the roasting space, a deodorizing unit(600), and a lighting unit. The deodorizing unit includes a filter and an exhaust fan, and removes a bad smell from air exhausted from the roasting space.
    • 目的:提供一种电烤炉,通过容易地控制电加热器的温度来防止食物被焦烧,并且通过安装除臭单元来排除排气中的恶臭。 构成:电烤炉(1)由多个格栅(110),多个格栅安装单元(120),加热器(200),用于控制加热器的功率控制单元,多个焙烧空间 内部的加热器,包括用于打开/关闭焙烧空间的门(530)的主体(500),除臭单元(600)和照明单元。 除臭单元包括过滤器和排气风扇,并且从从焙烧空间排出的空气中除去不良气味。
    • 3. 发明授权
    • 미생물의 동결건조방법
    • 미생물의동결건조방법
    • KR100390385B1
    • 2003-07-07
    • KR1020000037709
    • 2000-07-03
    • 장덕진김상종
    • 장덕진김상종이규호이동훈조장천박지은
    • C12N1/04
    • PURPOSE: A freeze-drying method of a microorganism is provided, thereby maintaining the survival rate of luminescent microorganism up to 50% or more and the rate of luminescence up to 45%. CONSTITUTION: The freeze-drying method of a microorganism is accomplished by adding 0.01M or more trehalose into a growth medium; inoculating a microorganism into the trehalose-containing growth medium; cultivating the microorganism with shacking; and freeze-drying the cultured medium, in which the microorganism is a luminescent microorganism; the cultivation is carried out for 1 to 30 minutes; and the trehalose is added to the cell during freeze-drying process.
    • 目的:提供一种微生物的冷冻干燥方法,由此将发光微生物的存活率保持在50%以上,发光率维持在45%以下。 组成:微生物的冷冻干燥方法通过将0.01M或更多的海藻糖加入生长培养基中完成; 将微生物接种到含海藻糖的生长培养基中; 摇晃培养微生物; 并冷冻干燥其中微生物是发光微生物的培养基; 培养进行1至30分钟; 并在冷冻干燥过程中将海藻糖加入到细胞中。
    • 5. 发明授权
    • 암모니아 산화 저해제 검출용 바이오센서
    • 用于检测氨氧化抑制剂的生物传感器
    • KR100555840B1
    • 2006-03-03
    • KR1020040039246
    • 2004-05-31
    • 장덕진
    • 장덕진춰이롱
    • C12Q1/02G01N33/569C12Q1/00
    • 본 발명은 암모니아 산화 저해제를 검출하기 위한 미생물 바이오센서에 관한 것으로, 더욱 상세하게는, 순수 배양된 니트로소모나스 유로파에아(
      Nitrosomonas europaea )의 세포를 MWCO 12,000~16,000 Da의 투석막에 진공 여과시키는 방법에 의하여 막에 고정하고, 상기 세포가 고정된 막을 DO 프로브(DO probe)에 부착한 다음, 고정된 미생물 세포의 산소 섭취율(oxygen uptake rate)을 측정함으로써 암모니아 산화 저해제를 검출하는 바이오센서에 관한 것이다.
      본 발명에 의한 바이오센서를 이용하여 암모니아 산화 저해제를 분석할 경우, CBOD 물질에 의한 분석 에러가 발생하지 않으므로 매우 특이성과 민감성이 높은 분석 결과를 제공하며, 온라인 모니터링이 가능하여 암모니아 산화에 대한 실시간 정보를 제공하므로 매우 신속하고 간편한 분석 결과를 제공한다.
      바이오센서, 니트로소모나스 유로파에아, 투석막, 순수 배양, 암모니아, 질산화
    • 6. 发明公开
    • 미생물의 동결건조방법
    • 微生物冷冻干燥方法
    • KR1020020004146A
    • 2002-01-16
    • KR1020000037709
    • 2000-07-03
    • 장덕진김상종
    • 장덕진김상종이규호이동훈조장천박지은
    • C12N1/04
    • PURPOSE: A freeze-drying method of a microorganism is provided, thereby maintaining the survival rate of luminescent microorganism up to 50% or more and the rate of luminescence up to 45%. CONSTITUTION: The freeze-drying method of a microorganism is accomplished by adding 0.01M or more trehalose into a growth medium; inoculating a microorganism into the trehalose-containing growth medium; cultivating the microorganism with shacking; and freeze-drying the cultured medium, in which the microorganism is a luminescent microorganism; the cultivation is carried out for 1 to 30 minutes; and the trehalose is added to the cell during freeze-drying process.
    • 目的:提供微生物的冷冻干燥方法,从而将发光微生物的存活率保持在50%以上,发光率高达45%。 构成:微生物的冷冻干燥方法是通过向生长培养基中加入0.01M以上的海藻糖而实现的; 将微生物接种到含海藻糖的生长培养基中; 用棚架培养微生物; 并冷冻干燥培养的培养基,其中微生物是发光微生物; 培养1〜30分钟; 并且在冷冻干燥过程中将海藻糖加入到细胞中。
    • 7. 发明公开
    • 아연분말 및 아연 폼을 이용한 수계의 질산성 질소와아질산성 질소의 제거방법
    • 使用锌粉和锌粉的硝酸和硝酸氮的脱氮方法
    • KR1020010069912A
    • 2001-07-25
    • KR1020010027249
    • 2001-05-18
    • 장덕진
    • 장덕진이규성
    • C02F1/58
    • PURPOSE: A removal method for nitrate nitrogen and nitrite nitrogen of river system using zinc powder and zinc foam is provided, which can denitrify nitrate nitrogen and nitrite nitrogen in river system more economically and effectively than the conventional method using aluminum, iron, tin, nickel and copper. The powder or foam of zinc can be reused by acid(2.5 % HCl) washing for 3-5 hours. CONSTITUTION: The method comprises as follows: (i) mix zinc powder of 1-53 micrometer in diameter with an additive such as ethyl alcohol and a binder selected from the group consisting of gum, wax, methyl cellulose, poly vinyl alcohol, hydroxy ethyl cellulose, polyvinyl butyral and polyethylene glycol; (ii) impregnate the mixture in a slurry state into sponge made of polyurethane; (iii) remove excess sludge in the sponge; (iv) dry at 60-120 deg.C; (v) burn the sponge at 200-250 deg.C for 2-4 hours; (vi) sinter the zinc powder at 400-420 deg.C for 10-15hours; and (vi) anneal the zinc powder to prepare foam.
    • 目的:提供使用锌粉和锌泡沫的河流系统硝酸氮和亚硝酸盐氮的去除方法,与常规方法相比,使用铝,铁,锡,镍,可以更经济有效地使硝酸盐氮和亚硝酸盐氮脱氮 和铜。 锌的粉末或泡沫可以通过酸(2.5%HCl)洗涤3-5小时来重复使用。 方法:该方法包括:(i)将直径为1-53微米的锌粉与乙醇和粘合剂等添加剂混合,所述粘合剂选自由树胶,蜡,甲基纤维素,聚乙烯醇,羟乙基 纤维素,聚乙烯醇缩丁醛和聚乙二醇; (ii)将淤浆状态的混合物浸渍在由聚氨酯制成的海绵中; (iii)去除海绵中多余的污泥; (iv)在60-120℃干燥; (v)将海绵在200-250摄氏度烧伤2-4小时; (vi)在400-420℃烧结锌粉10〜15小时; 和(vi)使锌粉末退火以制备泡沫。