会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 8. 发明授权
    • 내열성을 갖는 포스포리파제 A1의 돌연변이체 및 그의제조방법
    • 내열성을갖는포스포리파제A1의돌연변이체및그의제조방
    • KR100380804B1
    • 2003-04-18
    • KR1020000054116
    • 2000-09-15
    • 한국과학기술원
    • 이준식송재광
    • C12N15/55
    • C12N9/18C12Y301/01032
    • The present invention provides thermostable phospholipase TA3 and TA13 which are mutants of phospholipase A1 involving the hydrolysis/synthesis of phospholipids, the mutated genes encoding the same, microorganisms transformed with recombinant expression vectors comprising the mutated genes and a process for preparing phospholipase A1 mutants therefrom. The process for preparing phospholipase A1 mutant of the invention comprises the steps of: culturing E. coli strain transformed with a recombinant expression vector comprising a gene coding for phospholipase A1 mutant derived from Serratia sp.; and, isolating and purifying phospholipase A1 mutant from the culture. Since phospholipase A1 mutants of the invention have been improved in terms of thermal stability and enzyme activity as well, they can be practically applied to various biological process, pharmaceutics, cosmetics and food industries.
    • 本发明提供了热稳定的磷脂酶TA3和TA13,它们是涉及磷脂水解/合成的磷脂酶A1突变体,编码其的突变基因,用包含突变基因的重组表达载体转化的微生物和由其制备磷脂酶A1突变体的方法。 制备本发明的磷脂酶A1突变体的方法包括以下步骤:培养用包含编码来自沙雷氏菌属的磷脂酶A1突变体的基因的重组表达载体转化的大肠杆菌菌株; 并从培养物中分离和纯化磷脂酶A1突变体。 由于本发明的磷脂酶A1突变体在热稳定性和酶活性方面也有所改进,它们可以实际应用于各种生物过程,药剂学,化妆品和食品工业。
    • 9. 发明公开
    • 내열성을 갖는 포스포리파제 A1의 돌연변이체 및 그의제조방법
    • 具有热稳定性的磷脂酶A1突变体及其制备方法
    • KR1020020021424A
    • 2002-03-21
    • KR1020000054116
    • 2000-09-15
    • 한국과학기술원
    • 이준식송재광
    • C12N15/55
    • C12N9/18C12Y301/01032
    • PURPOSE: Provided are phospholipase TA3 and TA13 which are mutants of phospholipase A1 decomposing or synthesizing phospholipid and has thermostability. Also, provided are their coding genes, a recombinant vector containing the genes and a microorganism transformed with the vector. Further provided is a manufacturing method of phospholipase A1 mutant therewith. CONSTITUTION: The phospholipase TA3 and TA13 are derived from Serratia sp. strain MK1, and have nucleotide sequences of the SEQ ID NO:4 and SEQ ID NO:5 respectively and amino acid sequences of SEQ ID NO:6 and SEQ ID NO:7 respectively. The preparation comprises culturing E. coli transformants (Escherichia coliXL-Blue/pTA3, KCTC-18037P, Escherichia coliXL-Blue/pTA13, KCTC-18038P) transformed with vectors, pTA3 and pTA13, containing mutant genes of phospholipase A1 derived from Serratia sp.; separating the above phospholipase A1 mutants from the culture medium and purifying them.
    • 目的:提供分解或合成磷脂的磷脂酶A1的突变体的磷脂酶TA3和TA13,并具有热稳定性。 此外,提供它们的编码基因,含有该基因的重组载体和用载体转化的微生物。 进一步提供了与其突变的磷脂酶A1的制造方法。 构成:磷脂酶TA3和TA13来源于沙雷氏菌属 分别具有SEQ ID NO:4和SEQ ID NO:5的核苷酸序列和SEQ ID NO:6和SEQ ID NO:7的氨基酸序列。 该制剂包括用载体pTA3和pTA13转化的大肠杆菌转化体(Escherichia coli XL-Blue / pTA3,KCTC-18037P,大肠杆菌XL-Blue / pTA13,KCTC-18038P),其含有源自沙雷氏菌属的磷脂酶A1的突变基因。 ; 将上述磷脂酶A1突变体从培养基中分离并纯化。
    • 10. 发明授权
    • 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자
    • 衍生自荧光假单胞菌的基因,促进微生物中外源蛋白的分泌
    • KR100312456B1
    • 2001-11-03
    • KR1019990008512
    • 1999-03-13
    • 한국과학기술원
    • 이준식반재구안정훈
    • C12N15/31C12N15/67
    • C07K14/21
    • 본발명은미생물에서외래단백질을발현시켜세포밖 배양배지로효율적으로분비시킬수 있도록하는단백질을코딩하는슈도모나스플루오레슨스에서유래한분비촉진유전자, 그를포함하는재조합발현벡터와, 전기발현벡터에의하여형질전환된형질전환체, 및전기발현벡터로형질전환된미생물을배양하고외래단백질의발현을유도하여, 발현된외래단백질이세포밖 배양액으로분비되도록유도하는단계를포함하는외래단백질의생산방법에관한것이다. 본발명에따라일반분비경로에비하여훨씬간단하고효과적으로외래단백질을세포밖으로분비하게할 수있는분비촉진유전자에의한분비시스템이새롭게개발되었으며, 특히슈도모나스플루오레슨스에서외래단백질을세포밖으로분비시키는기능을담당하는분비촉진유전자를규명함으로써, 이분비시스템을이용하여외래단백질을효율적으로발현시키고, 이를산업적으로다양하게이용하는목적에유용하게사용할수 있다.