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    • 2. 发明授权
    • 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자
    • 衍生自荧光假单胞菌的基因,促进微生物中外源蛋白的分泌
    • KR100312456B1
    • 2001-11-03
    • KR1019990008512
    • 1999-03-13
    • 한국과학기술원
    • 이준식반재구안정훈
    • C12N15/31C12N15/67
    • C07K14/21
    • 본발명은미생물에서외래단백질을발현시켜세포밖 배양배지로효율적으로분비시킬수 있도록하는단백질을코딩하는슈도모나스플루오레슨스에서유래한분비촉진유전자, 그를포함하는재조합발현벡터와, 전기발현벡터에의하여형질전환된형질전환체, 및전기발현벡터로형질전환된미생물을배양하고외래단백질의발현을유도하여, 발현된외래단백질이세포밖 배양액으로분비되도록유도하는단계를포함하는외래단백질의생산방법에관한것이다. 본발명에따라일반분비경로에비하여훨씬간단하고효과적으로외래단백질을세포밖으로분비하게할 수있는분비촉진유전자에의한분비시스템이새롭게개발되었으며, 특히슈도모나스플루오레슨스에서외래단백질을세포밖으로분비시키는기능을담당하는분비촉진유전자를규명함으로써, 이분비시스템을이용하여외래단백질을효율적으로발현시키고, 이를산업적으로다양하게이용하는목적에유용하게사용할수 있다.
    • 3. 发明公开
    • 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자
    • PSEUDOMONAS荧光衍生基因促进出口外源蛋白
    • KR1020000060322A
    • 2000-10-16
    • KR1019990008512
    • 1999-03-13
    • 한국과학기술원
    • 이준식반재구안정훈
    • C12N15/31C12N15/67
    • C07K14/21
    • PURPOSE: A novel method for producing a pseudomonas fluorescence derived gene which promotes exporting of exogenous protein, is provided to give convenience in secreting the protein effectively out of a cell. CONSTITUTION: The gene promoting exporting of exogenous protein is characterized by being derived from pseudomonas fluorescence, and comprising nucleotides of sequence number 4, number 5 and number 6 orderly. In particular, the method thereof comprises identifying the gene which promotes exporting of exogenous protein from pseudomonas fluorescence SIK W1(KCTC 2689P) outwardly to environment rather than the subject cell. Also, an expression vector of the gene, pABC-ACYC, is characterized by having specific gene map, as shown in figure 1. Further, provided is E.coli XL1-Blue/pABC-ACYC (KCTC 0586BP) transformed by the expression vector pABC-ACYC.
    • 目的:提供促进外源蛋白质输出的假单胞菌荧光衍生基因的新方法,以便有效地从细胞中分泌蛋白质。 构成:促进外源蛋白质输出的基因的特征在于衍生自假单胞菌荧光,其序列号为4,5和6的核苷酸有序。 特别地,其方法包括鉴定促进外源蛋白质从假单胞菌荧光SIK W1(KCTC 2689P)向外输出到环境而不是主体细胞的基因。 此外,基因表达载体pABC-ACYC的特征在于具有特异性基因图,如图1所示。此外,提供了通过表达载体转化的大肠杆菌XL1-Blue / pABC-ACYC(KCTC 0586BP) PABC-ACYC。