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    • 10. 发明公开
    • 신규 박테리아 용해 단백질 및 그의 용도
    • 新型细菌蛋白质及其用途
    • KR1020130060605A
    • 2013-06-10
    • KR1020110126747
    • 2011-11-30
    • 전남대학교산학협력단
    • 최현일민정준정재호유상렬임정아
    • C07K14/255C12N15/31C12N15/63A61P35/00
    • C07K14/255A61K47/42A61K47/62C12N15/63
    • PURPOSE: A novel polypeptide and a polynucleotide encoding the same are provided to properly control bacterial lysis and to effectively release a drug transferred by therapeutic bacteria to a lesion. CONSTITUTION: A polypeptide has holin non-dependent cell lysis activities with 70% or more homology with an amino acid sequence of sequence number 3. The polypeptide has 70% or more homology with an amino acid sequence of sequence number 1. A polynucleotide encodes the polypeptide. A vector contains the polynucleotide. A host cell is transformed with the vector. [Reference numerals] (AA) pLT1::ORF1, Control group; (BB) pLT1::ORF1, Induced; (CC,FF,II,LL,OO,RR) Time(hours); (DD) pLT35-1, Control group; (EE) pLT35-1, Induced; (GG) pLT1::ORF2, Control group; (HH) pLT1::ORF2, Induced; (JJ) pLT33, Control group; (KK) pLT33, Induced; (MM) pLT1::ORF3, Control group; (NN) pLT1::ORF3, Induced; (PP) pLT34, Control group; (QQ) pLT34, Induced
    • 目的:提供新的多肽和编码该多肽的多核苷酸以适当地控制细菌裂解并有效地将由治疗性细菌转移的药物释放到病变处。 构成:多肽具有与序列号3的氨基酸序列具有70%或更高同源性的完全非依赖性细胞裂解活性。多肽与序列号1的氨基酸序列具有70%或更高的同源性。多核苷酸编码 多肽。 载体含有多核苷酸。 用载体转化宿主细胞。 (标号)(AA)pLT1 :: ORF1,对照组; (BB)pLT1 :: ORF1,诱导; (CC,FF,II,LL,OO,RR)时间(小时); (DD)pLT35-1,对照组; (EE)pLT35-1,诱导; (GG)pLT1 :: ORF2,对照组; (HH)pLT1 :: ORF2,诱导; (JJ)pLT33,对照组; (KK)pLT33,诱导; (MM)pLT1 :: ORF3,对照组; (NN)pLT1 :: ORF3,诱导; (PP)pLT34,对照组; (QQ)pLT34,诱导