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1. 发明申请

US20120322676A1 COMPOSITIONS AND METHODS FOR DETECTION OF CRONOBACTER SPP. AND CRONOBACTER SPECIES AND STRAINS 审中-公开
标题翻译：用于检测CRONOBACTER SPP的组合物和方法。和克隆物种和菌株
公开(公告)号：US20120322676A1
公开(公告)日：2012-12-20
申请号：US13495748
申请日：2012-06-13
申请人： Yongmei JI , Sheung-Mei ("Rita") Shih , Lovorka Degoricija , Zhaohui Zhou , Craig Cummings , Manohar Furtado , Pius Brzoska , Angela Burrell , Harrison Leong , Xingwang Fang , Mangkey Bounpheng , Rohan Shah
发明人： Yongmei JI , Sheung-Mei ("Rita") Shih , Lovorka Degoricija , Zhaohui Zhou , Craig Cummings , Manohar Furtado , Pius Brzoska , Angela Burrell , Harrison Leong , Xingwang Fang , Mangkey Bounpheng , Rohan Shah
IPC分类号： C12Q1/68 , C40B30/04 , G01N21/76 , C07H21/04 , G01N27/62
CPC分类号： C12Q1/689 , C12R1/01
摘要： Disclosed are genomic sequences for nine strains of Cronobacter spp. (C. sakazakii—696, 701, 680; C. malonaticus—507, 681; C. turicensis—564; C. muytjensii—530; C. dublinensis—582; C. genomosp1—581) and compositions, methods, and kits for detecting, identifying and distinguishing Cronobacter spp. strains from each other and from non-Cronobacter spp. strains. Some embodiments describe isolated nucleic acid compositions unique to certain Cronobacter strains as well as compositions that are specific to all Cronobacter spp. Primer and probe compositions and methods of use of primers and probes are also provided. Kits for identification of Cronobacter spp. are also described. Some embodiments relate to computer software methods for setting a control based threshold for analysis of PCR data.
摘要翻译：公开了九个克罗杆菌属菌株的基因组序列。（C.sakazakii-696,701,680; C. malonaticus-507,681; C. turicensis-564; C. muytjensii-530; C.dublinensis-582; C.基因组sp1-581）和组合物，方法和试剂盒用于检测，识别和鉴别克罗杆菌属。菌株彼此和非克罗杆菌属。菌株。一些实施方案描述了某些克罗杆菌菌株特有的分离的核酸组合物以及对所有克罗杆菌属特异性的组合物。还提供引物和探针组合物以及引物和探针的使用方法。克隆杆菌属鉴定试剂盒也被描述。一些实施例涉及用于设置用于PCR数据分析的基于控制的阈值的计算机软件方法。

2. 发明申请

US20130157277A1 DEVICE FOR CAPTURE AND LYSIS OF MICROORGANISMS FROM LIQUIDS AND METHODS OF USE THEREOF 有权
公开(公告)号：US20130157277A1
公开(公告)日：2013-06-20
申请号：US13819240
申请日：2011-09-01
申请人： Kevin Hacker , Gregory Govoni , Nikolay Sergeev , Elena Bolchacova , Maxim Brevnov , Manohar Furtado , Johnie Young , Jim Nurse , Mareila Cuadras
发明人： Kevin Hacker , Gregory Govoni , Nikolay Sergeev , Elena Bolchacova , Maxim Brevnov , Manohar Furtado , Johnie Young , Jim Nurse , Mareila Cuadras
IPC分类号： B01D29/13 , C12Q1/68
CPC分类号： C12N15/1003 , B01D29/13 , C12N1/066 , C12Q1/68 , C12Q1/689 , C12Q1/6895 , G01N21/75
摘要： Devices and methods for detecting microbial contaminants, such as bacteria and fungi, in fluids such as drinking water, pharmaceutical solutions and tissue culture media are provided. More particularly, provided are filtration devices for capture and processing of microorganisms from fluids, and improved methods for recovery, lysis and detection of microorganisms based on a combination of physical disruption with small beads and lysis solutions.

3. 发明申请

US20130137589A2 ANALYSIS OF Y-CHROMOSOME STR MARKERS 审中-公开
公开(公告)号：US20130137589A2
公开(公告)日：2013-05-30
申请号：US12880040
申请日：2010-09-10
申请人： Rixun FANG , Manohar FURTADO , Manfred KAYSER , Kaye BALLANTYNE
发明人： Rixun FANG , Manohar FURTADO , Manfred KAYSER , Kaye BALLANTYNE
IPC分类号： C40B30/00 , G01N33/559 , H01J49/26 , G01N27/447 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6888 , C12Q2600/156 , C12Q2600/16 , C12Q2600/172
摘要： The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.

4. 发明授权

US08445209B2 Nucleic acid compositions and nucleic acid amplification based methods for detecting E. coli O157:H7 有权
标题翻译：用于检测大肠杆菌O157：H7的核酸组合物和基于核酸扩增的方法
公开(公告)号：US08445209B2
公开(公告)日：2013-05-21
申请号：US12780707
申请日：2010-05-14
申请人： Paolo Vatta , Olga Petrauskene , Manohar Furtado , Pius Brzoska , Lily Wong , Melissa Barker , Craig A. Cummings
发明人： Paolo Vatta , Olga Petrauskene , Manohar Furtado , Pius Brzoska , Lily Wong , Melissa Barker , Craig A. Cummings
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/689 , C12Q2600/142
摘要： Disclosed are primer and probe compositions, PCR assays, methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from a variety of samples. In some embodiments, methods for specifically detecting E. coli O157:H7 comprise: hybridizing at least a first pair of polynucleotide primers to at least a first target polynucleotide sequence, hybridizing at least a second pair of polynucleotide primers to at least a second target polynucleotide sequence, amplifying said at least first and said at least second target polynucleotide sequences, and detecting said at least first and said at least second amplified target polynucleotide sequence products, wherein the detection of both the first amplified target polynucleotide sequence product and the second amplified target polynucleotide sequence product is indicative of the presence of E. coli O157:H7 in a sample and not E. coli O55:H7.
摘要翻译：公开了用于特异性检测来自各种样品的大肠杆菌O157：H7而不是大肠杆菌O55：H7的引物和探针组合物，PCR测定，方法和试剂盒。在一些实施方案中，用于特异性检测大肠杆菌O157：H7的方法包括：使至少第一对多核苷酸引物与至少第一靶多核苷酸序列杂交，将至少第二对多核苷酸引物杂交至至少第二靶多核苷酸序列，扩增所述至少第一和所述至少第二个靶多核苷酸序列，以及检测所述至少第一和所述至少第二扩增的目标多核苷酸序列产物，其中检测第一扩增的靶多核苷酸序列产物和第二扩增的靶多核苷酸序列产物指示样品中大肠杆菌O157：H7的存在，而不是大肠杆菌O55：H7。

5. 发明申请

US20120015370A1 MULTIPLEX COMPOSITIONS AND METHODS FOR QUANTIFICATION OF HUMAN NUCLEAR DNA AND HUMAN MALE DNA AND DETECTION OF PCR INHIBITORS 有权
标题翻译：多重组合物和人核DNA定量鉴定方法及人类DNA的检测及PCR抑制剂的检测
公开(公告)号：US20120015370A1
公开(公告)日：2012-01-19
申请号：US13219400
申请日：2011-08-26
申请人： Jaiprakash G. SHEWALE , Manohar Furtado , Pius Brzoska , Maura Barbisin , Rixun Fang , Michael Malicdem , Cristin O'Shea
发明人： Jaiprakash G. SHEWALE , Manohar Furtado , Pius Brzoska , Maura Barbisin , Rixun Fang , Michael Malicdem , Cristin O'Shea
IPC分类号： C12Q1/68 , C12N15/63 , C07H21/04
CPC分类号： C12Q1/6879 , C12Q1/6851 , C12Q1/686 , C12Q1/6888 , C12Q2600/16 , C12Q2600/166 , C12Q2537/143
摘要： The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.
摘要翻译：本发明涉及一种用于同时定量生物样品中的人核DNA和人类雄性DNA的方法，同时还在单次反应中检测出PCR抑制剂的存在。多重定量方法还提供存在于生物样品中的人类核和雄性DNA的比例。这种样品表征对于在下游分子技术如基因分型中获得有效和准确的结果是有用的。

6. 发明申请

US20110263437A1 Analysis of Y-Chromosome STR Markers 审中-公开
标题翻译： Y染色体STR标记分析
公开(公告)号：US20110263437A1
公开(公告)日：2011-10-27
申请号：US12880040
申请日：2010-09-10
申请人： Rixun FANG , Manohar Furtado , Manfred Kayser , Kaye Ballantyne
发明人： Rixun FANG , Manohar Furtado , Manfred Kayser , Kaye Ballantyne
IPC分类号： C40B30/00 , G01N33/559 , H01J49/26 , G01N27/447 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6888 , C12Q2600/156 , C12Q2600/16 , C12Q2600/172
摘要： The methods and compositions provided herein relate to the discovery of 13 STR are markers found on the human Y chromosome with surprisingly high mutation rates when compared with 173 other Y-STR markers known today, including those that are in common use in forensics. In addition to theoretical expectations based on elevated mutation rates, these 13 rapidly-mutating (RM) Y-STRs proved to be suitable for differentiating between closely related males, as well as between more distant male relatives and much more so than the most-commonly used Y-STRs in forensics. Our new set of RM-Y-STRs is expected to overcome the current dilemma of Y-chromosome analysis in forensic applications from current male lineage identification towards male individual identification in many cases, due to their extraordinary mutation properties as discovered here. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.
摘要翻译：本文提供的方法和组合物涉及当与目前已知的173种其它Y-STR标记（包括在法医学中常用的那些）相比时，发现13 STR是在人Y染色体上发现的标记，具有惊人的高突变率。除了基于提高的突变率的理论预期之外，这13个快速突变（RM）Y-STR证明适用于区分密切相关的男性，以及更远的男性亲属之间的差异，并且比最常见的使用Y-STR进行取证。我们新推出的RM-Y-STR可以克服当前男性谱系识别对男性个体识别的法医应用中Y染色体分析目前的困境，因为在这里发现了非凡的突变特性。本发明的实施方案包括用于等位基因测定快速突变的Y-STR标记的方法，用于分析快速突变的Y-STR标记的扩增引物，用于分析快速突变的Y-STR标记的等位基因梯度和用于分析的试剂盒的快速突变的Y-STR标记。

7. 发明申请

US20110111403A1 MULTI-PRIMER ASSAY FOR MYCOPLASMA DETECTION 审中-公开
标题翻译：用于MYCOPLASMA检测的多重检测方法
公开(公告)号：US20110111403A1
公开(公告)日：2011-05-12
申请号：US12916420
申请日：2010-10-29
申请人： Olga PETRAUSKENE , Pius Brzoska , Somaya Bit , Jen-Kuei Liu , Robert Tebbs , Manohar Furtado
发明人： Olga PETRAUSKENE , Pius Brzoska , Somaya Bit , Jen-Kuei Liu , Robert Tebbs , Manohar Furtado
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.
摘要翻译：公开了一种多引物扩增测定法，用于利用在单个容器中与样品接触的多个寡核苷酸引物检测支原体物种和密切相关物种的检测扩增产物，其中扩增产物的存在表示支原体在样品中。

8. 发明授权

US08445657B2 Multiplex compositions and methods for quantification of human nuclear DNA and human male DNA and detection of PCR inhibitors 有权
标题翻译：用于定量人核DNA和人类雄性DNA的多重组合物和方法以及PCR抑制剂的检测
公开(公告)号：US08445657B2
公开(公告)日：2013-05-21
申请号：US13219400
申请日：2011-08-26
申请人： Jaiprakash Shewale , Manohar Furtado , Pius Brzoska , Maura Barbisin , Rixun Fang , Michael Malicdem , Cristin O'Shea
发明人： Jaiprakash Shewale , Manohar Furtado , Pius Brzoska , Maura Barbisin , Rixun Fang , Michael Malicdem , Cristin O'Shea
IPC分类号： C07H21/04
CPC分类号： C12Q1/6879 , C12Q1/6851 , C12Q1/686 , C12Q1/6888 , C12Q2600/16 , C12Q2600/166 , C12Q2537/143
摘要： The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.
摘要翻译：本发明涉及一种用于同时定量生物样品中的人核DNA和人类雄性DNA的方法，同时还在单次反应中检测出PCR抑制剂的存在。多重定量方法还提供存在于生物样品中的人类核和雄性DNA的比例。这种样品表征对于在下游分子技术如基因分型中获得有效和准确的结果是有用的。

9. 发明申请

US20130122505A1 COMPOSITIONS AND METHODS FOR DETECTION OF MULTIPLE MICROORGANISMS 审中-公开
标题翻译：用于检测多种微生物的组合物和方法
公开(公告)号：US20130122505A1
公开(公告)日：2013-05-16
申请号：US13594682
申请日：2012-08-24
申请人： Robert TEBBS , Craig Cummings , Pius Brzoska , Sharon Matheny , Catherine O'Connell , Manohar Furtado , Rixun Fang , Olga Petrauskene , Sueh-Ning Liew , Michael Schumaker
发明人： Robert TEBBS , Craig Cummings , Pius Brzoska , Sharon Matheny , Catherine O'Connell , Manohar Furtado , Rixun Fang , Olga Petrauskene , Sueh-Ning Liew , Michael Schumaker
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/689 , C12Q2600/16
摘要： The present teachings describe compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as a shiga toxin (stx) or an eae. In some embodiments, compositions, methods and kits can detect and identify individual strains and serotypes of shiga toxin producing microorganisms. Some embodiments describe compositions, methods and kits for detecting STEC microbes. Workflows for multiple microbe detection and identification are also described.
摘要翻译：本教导描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。一些实施方案涉及检测产生毒力因子（例如志贺毒素（stx）或eae）的一种或多种微生物。在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体菌株和血清型。一些实施方案描述了用于检测STEC微生物的组合物，方法和试剂盒。还描述了用于多个微生物检测和鉴定的工作流程。

10. 发明授权

US08012691B2 Multiplex compositions and methods for quantification of human nuclear DNA and human male DNA and detection of PCR inhibitors 有权
标题翻译：用于定量人核DNA和人类雄性DNA的多重组合物和方法以及PCR抑制剂的检测
公开(公告)号：US08012691B2
公开(公告)日：2011-09-06
申请号：US12034634
申请日：2008-02-20
申请人： Jaiprakash G. Shewale , Manohar Furtado , Pius Brzoska , Maura Barbisin , Rixun Fang , Michael Malicdem , Cristin O'Shea
发明人： Jaiprakash G. Shewale , Manohar Furtado , Pius Brzoska , Maura Barbisin , Rixun Fang , Michael Malicdem , Cristin O'Shea
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6879 , C12Q1/6851 , C12Q1/686 , C12Q1/6888 , C12Q2600/16 , C12Q2600/166 , C12Q2537/143
摘要： The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.
摘要翻译：本发明涉及一种用于同时定量生物样品中的人核DNA和人类雄性DNA的方法，同时还在单次反应中检测出PCR抑制剂的存在。多重定量方法还提供存在于生物样品中的人类核和雄性DNA的比例。这种样品表征对于在下游分子技术如基因分型中获得有效和准确的结果是有用的。

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