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1. 发明授权

US08702950B2 Device for electrophoresis, device for transfer, device for electrophoresis and transfer, chip for electrophoresis and transfer, and method for electrophoresis, method for transfer, and method for electrophoresis and transfer 失效
标题翻译：电泳装置，转移装置，电泳和转印装置，电泳和转印用芯片，电泳方法，转印方法及电泳转印方法
公开(公告)号：US08702950B2
公开(公告)日：2014-04-22
申请号：US13607844
申请日：2012-09-10
申请人： Yuji Maruo , Yutaka Unuma , Atsunori Hiratsuka , Hideki Kinoshita , Kenji Yokoyama , Koji Sakairi , Satonari Akutsu
发明人： Yuji Maruo , Yutaka Unuma , Atsunori Hiratsuka , Hideki Kinoshita , Kenji Yokoyama , Koji Sakairi , Satonari Akutsu
IPC分类号： G01N27/26 , B01D57/02
CPC分类号： G01N27/44704 , B01L3/50273
摘要： A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.
摘要翻译：用于电泳的装置将电压施加到与多个电导体接触的介质，使得相邻导体的电位在一定范围内。这样可以防止电泳速度下降。用于电泳和转印的装置包括具有彼此绝缘并沿特定方向布置的多个电极区域的电极。这允许提供用于电泳和转移的实用且易于使用的装置。用于传送的装置将施加的电压或施加的电压持续时间改变到某个位置到另一位置。这样可以提高传输效率。

2. 发明申请

US20130015069A1 DEVICE FOR ELECTROPHORESIS, DEVICE FOR TRANSFER, DEVICE FOR ELECTROPHORESIS AND TRANSFER, CHIP FOR ELECTROPHORESIS AND TRANSFER, AND METHOD FOR ELECTROPHORESIS, METHOD FOR TRANSFER, AND METHOD FOR ELECTROPHORESIS AND TRANSFER 失效
标题翻译：用于电泳的装置，用于转印的装置，用于电泳和转印的装置，用于电泳和转印的芯片，以及用于电泳的方法，用于转移的方法，以及用于电泳和转移的方法
公开(公告)号：US20130015069A1
公开(公告)日：2013-01-17
申请号：US13607844
申请日：2012-09-10
申请人： Yuji MARUO , Yutaka Unuma , Atsunori Hiratsuka , Hideki Kinoshita , Kenji Yokoyama , Koji Sakairi , Satonari Akutsu
发明人： Yuji MARUO , Yutaka Unuma , Atsunori Hiratsuka , Hideki Kinoshita , Kenji Yokoyama , Koji Sakairi , Satonari Akutsu
IPC分类号： B01D57/02
CPC分类号： G01N27/44704 , B01L3/50273
摘要： A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.
摘要翻译：用于电泳的装置将电压施加到与多个电导体接触的介质，使得相邻导体的电位在一定范围内。这样可以防止电泳速度下降。用于电泳和转印的装置包括具有彼此绝缘并沿特定方向布置的多个电极区域的电极。这允许提供用于电泳和转移的实用且易于使用的装置。用于传送的装置将施加的电压或施加的电压持续时间改变到某个位置到另一位置。这样可以提高传输效率。

3. 发明申请

US20110233062A1 Biological Molecule Separation Apparatus 失效
标题翻译：生物分子分离装置
公开(公告)号：US20110233062A1
公开(公告)日：2011-09-29
申请号：US13073835
申请日：2011-03-28
申请人： Koji Sakairi , Satonari Akutsu , Atsunori Hiratsuka , Kenji Yokoyama , Yuji Maruo , Hiroshi Ohki
发明人： Koji Sakairi , Satonari Akutsu , Atsunori Hiratsuka , Kenji Yokoyama , Yuji Maruo , Hiroshi Ohki
IPC分类号： C25B7/00
CPC分类号： G01N27/44769
摘要： A biological molecule separation apparatus for separating a biological molecule of one embodiment of the present invention has a buffer solution chamber receiving a buffer solution, a separation electrode arranged in the buffer solution chamber, a biological molecule separation medium for separating a biological molecule, a biological molecule adsorption film for adsorbing a biological molecule, a pair of transfer electrodes having a plurality of line shaped conductors extending in a direction perpendicular to a first direction, the conductors being arranged in the direction perpendicular to the first direction, and a separation part which holds the transfer electrode, the biological molecule adsorption film, the medium and the transfer electrode, wherein the transfer electrode, the biological molecule adsorption film, the medium and the transfer electrode are stacked in this order.
摘要翻译：本发明的一个实施方案的分离生物分子的生物分子分离装置具有接收缓冲溶液的缓冲溶液室，配置在缓冲溶液室中的分离电极，分离生物分子的生物分子分离介质，生物分子分离装置用于吸附生物分子的分子吸附膜，具有沿与第一方向垂直的方向延伸的多个线状导体的一对转移电极，导体沿垂直于第一方向的方向排列，分离部保持转移电极，生物分子吸附膜，介质和转移电极，其中转移电极，生物分子吸附膜，介质和转移电极按此顺序堆叠。

4. 发明授权

US07964077B2 Automated two-dimensional electrophoresis apparatus and instrument constituting the apparatus 有权
标题翻译：构成装置的自动二维电泳装置和仪器
公开(公告)号：US07964077B2
公开(公告)日：2011-06-21
申请号：US11510838
申请日：2006-08-28
申请人： Yuji Maruo , Katsuyoshi Takahashi , Yutaka Unuma , Keisuke Usui , Hideki Kinoshita , Kisho Shiseki , Yoshio Suzuki , Atsunori Hiratsuka , Hiroyuki Fukui , Kenji Yokoyama , Ichiji Namatame , Kouhei Yodoya , Yasuhiro Ogawa , Koji Sakairi , Chie Hayashida , Satonari Akutsu , Kazuyoshi Yano , Isao Karube
发明人： Yuji Maruo , Katsuyoshi Takahashi , Yutaka Unuma , Keisuke Usui , Hideki Kinoshita , Kisho Shiseki , Yoshio Suzuki , Atsunori Hiratsuka , Hiroyuki Fukui , Kenji Yokoyama , Ichiji Namatame , Kouhei Yodoya , Yasuhiro Ogawa , Koji Sakairi , Chie Hayashida , Satonari Akutsu , Kazuyoshi Yano , Isao Karube
IPC分类号： G01N27/26 , G01N27/447
CPC分类号： G01N27/44778
摘要： The present invention is to provide a sample separation instrument used in a sample separation apparatus which includes: holding means for holding a first medium supporter which supports a first medium; and driving means for moving fixing means or the holding means in a direction parallel or perpendicular to a plane whose sides extend in the first direction and in the second directions. The sample separation instrument includes an insulator for storing a second medium which allows a sample separated in the first medium in the first direction to be further separated in the second direction different from the first direction, wherein: the insulator includes a first opening and a second opening each of which defines the second direction in which the second medium is electrified, and the second opening has a shape which allows the first medium containing the separated sample to be attached to the second medium along the first direction. The present invention realizes automation of the two-dimensional electrophoresis to enhance the convenience of the two-dimensional electrophoresis, to less depend on the operator's skill, and to enhance the reproducibility of the electrophoresis result.
摘要翻译：本发明提供一种在样品分离装置中使用的样品分离装置，其包括：保持装置，用于保持支撑第一介质的第一介质支持体; 以及用于在平行或垂直于其侧面沿第一方向和第二方向延伸的平面的方向上移动固定装置或保持装置的驱动装置。样品分离装置包括用于存储第二介质的绝缘体，其允许在第一方向上在第一介质中分离的样品在与第一方向不同的第二方向上进一步分离，其中：绝缘体包括第一开口和第二开口其中每个开口限定第二介质带电的第二方向，第二开口具有允许包含分离样品的第一介质沿着第一方向附着到第二介质的形状。本发明实现了二维电泳的自动化，以增强二维电泳的便利性，较少依赖于操作者的技能，并提高电泳结果的再现性。

5. 发明申请

US20070278102A1 Electrophoresis Apparatus and Device Therefor 有权
标题翻译：电泳装置及其装置
公开(公告)号：US20070278102A1
公开(公告)日：2007-12-06
申请号：US11663646
申请日：2006-09-05
申请人： Chie Hayashida , Koji Sakairi , Katsuyoshi Takahashi , Yuji Maruo , Michinobu Mieda , Yutaka Unuma
发明人： Chie Hayashida , Koji Sakairi , Katsuyoshi Takahashi , Yuji Maruo , Michinobu Mieda , Yutaka Unuma
IPC分类号： G01N27/447 , B01D57/02 , G01N21/64
CPC分类号： G01N27/44704 , B01D57/02 , G01N21/6428
摘要： There is provided an electrophoresis device (100) including an insulator (10) that includes: a first-separating-medium storing section (4′) for storing therein a first separating medium (4); a first opening (7) and a second opening (8) that are in communication with the first-separating-medium storing section (4′) and for defining a direction of separation on the first separating medium (4); and a third opening (9) that is covered with a penetrable insulating thin film (3), wherein the first separating medium storing section (4′) is in communication with outside via the first opening (7) and the second opening (8). There is also provided an electrophoresis apparatus including the electrophoresis device (100). This realizes an electrophoresis apparatus, and a device therefor, that enables an operator to sample separated proteins without ever making contact with the electrophoresed gel, and that can easily be used with various types of analyses, thereby improving convenience of electrophoresis.
摘要翻译：提供了一种包括绝缘体（10）的电泳装置（100），其包括：用于在其中存储第一分离介质（4）的第一分离介质存储部分（4'）; 第一开口（7）和第二开口（8），其与第一分离介质存储部分（4'）连通并且用于限定第一分离介质（4）上的分离方向; 和覆盖有可穿透绝缘薄膜（3）的第三开口（9），其中第一分离介质容纳部分（4'）经由第一开口（7）和第二开口（8）与外部连通，。还提供了包括电泳装置（100）的电泳装置。这实现了一种电泳装置及其装置，其使得操作者能够对分离的蛋白质进行取样，而不会与电泳凝胶接触，并且可以容易地与各种类型的分析一起使用，从而提高电泳的方便性。

6. 发明申请

US20140076727A1 ELECTROPHORESIS-GEL-FORMING MONOMER SOLUTION, SOLUTION EJECTING METHOD, METHOD FOR FORMING ELECTROPHORESIS GEL, ELECTROPHORESIS GEL, AND ELECTROPHORESIS REACTION INSTRUMENT 审中-公开
标题翻译：电泳 - 凝胶成型单体溶液，溶液喷射方法，形成电泳凝胶的方法，电泳凝胶和电泳反应仪
公开(公告)号：US20140076727A1
公开(公告)日：2014-03-20
申请号：US14007748
申请日：2012-03-27
申请人： Hiroshi Ohki , Hiroshi Yamaki , Yutaka Unuma , Yuji Maruo , Tsuyoshi Tanaka
发明人： Hiroshi Ohki , Hiroshi Yamaki , Yutaka Unuma , Yuji Maruo , Tsuyoshi Tanaka
IPC分类号： G01N27/447
CPC分类号： G01N27/447 , G01N27/44747
摘要： A solution ejecting method includes a first ejection step of ejecting an electrophoresis-gel-forming monomer solution containing at least a monomer that forms a gel structure and a gel polymerization accelerator that activates a gel polymerization initiator and a second ejection step of ejecting a gel polymerization initiating solution containing the gel polymerization initiator onto the electrophoresis-gel-forming monomer solution.
摘要翻译：溶液喷射方法包括：第一喷射步骤，喷射至少含有形成凝胶结构的单体的电泳 - 凝胶形成单体溶液和激活凝胶聚合引发剂的凝胶聚合促进剂和喷射凝胶聚合的第二喷射步骤将引发含有凝胶聚合引发剂的溶液引入电泳 - 凝胶形成单体溶液。

7. 发明申请

US20110114485A1 ELECTROPHORESIS APPARATUS AND ELECTROPHORESIS METHOD 有权
标题翻译：电泳装置和电泳方法
公开(公告)号：US20110114485A1
公开(公告)日：2011-05-19
申请号：US13054271
申请日：2009-07-15
申请人： Koji Sakairi , Satonari Akutsu , Yuji Maruo , Takateru Matsunaga , Yutaka Unuma , Atsunori Hiratsuka , Hideki Kinoshita , Nao Sakaguchi , Kenji Yokoyama
发明人： Koji Sakairi , Satonari Akutsu , Yuji Maruo , Takateru Matsunaga , Yutaka Unuma , Atsunori Hiratsuka , Hideki Kinoshita , Nao Sakaguchi , Kenji Yokoyama
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44773
摘要： An electrophoresis apparatus includes a sample separating section for containing a sample separating medium for separating a sample in a horizontal direction, the sample separating section containing the sample separating medium in such a manner that the sample separating medium has an exposed portion at least one end of a surface of the sample separating medium, the surface being in parallel with the horizontal direction; and medium connecting means for connecting a sample containing medium to the sample separating medium at a connecting region, the sample containing medium containing a sample, and the connecting region satisfying a particular equation. By this, an electrophoresis technique with better accuracy than conventional arts can be provided.
摘要翻译：电泳装置包括样品分离部分，用于容纳用于在水平方向上分离样品的样品分离介质，样品分离部分包含样品分离介质，使得样品分离介质具有暴露部分，至少一端所述样品分离介质的表面与所述水平方向平行; 以及介质连接装置，用于在连接区域连接包含样品的介质到样品分离介质，所述样品包含含有样品的介质和满足特定等式的连接区域。由此，可以提供比现有技术更好的精度的电泳技术。

8. 发明授权

US08419916B2 Biological molecule separation apparatus 失效
标题翻译：生物分子分离装置
公开(公告)号：US08419916B2
公开(公告)日：2013-04-16
申请号：US13073835
申请日：2011-03-28
申请人： Koji Sakairi , Satonari Akutsu , Atsunori Hiratsuka , Kenji Yokoyama , Yuji Maruo , Hiroshi Ohki
发明人： Koji Sakairi , Satonari Akutsu , Atsunori Hiratsuka , Kenji Yokoyama , Yuji Maruo , Hiroshi Ohki
IPC分类号： G01N27/26
CPC分类号： G01N27/44769
摘要： A biological molecule separation apparatus for separating a biological molecule of one embodiment of the present invention has a buffer solution chamber receiving a buffer solution, a separation electrode arranged in the buffer solution chamber, a biological molecule separation medium for separating a biological molecule, a biological molecule adsorption film for adsorbing a biological molecule, a pair of transfer electrodes having a plurality of line shaped conductors extending in a direction perpendicular to a first direction, the conductors being arranged in the direction perpendicular to the first direction, and a separation part which holds the transfer electrode, the biological molecule adsorption film, the medium and the transfer electrode, wherein the transfer electrode, the biological molecule adsorption film, the medium and the transfer electrode are stacked in this order.
摘要翻译：本发明的一个实施方案的分离生物分子的生物分子分离装置具有接收缓冲溶液的缓冲溶液室，配置在缓冲溶液室中的分离电极，分离生物分子的生物分子分离介质，生物分子分离装置用于吸附生物分子的分子吸附膜，具有沿与第一方向垂直的方向延伸的多个线状导体的一对转移电极，导体沿垂直于第一方向的方向排列，分离部保持转移电极，生物分子吸附膜，介质和转移电极，其中转移电极，生物分子吸附膜，介质和转移电极按此顺序堆叠。

9. 发明申请

US20120273360A1 INSTRUMENT FOR ELECTROPHORESIS AND ELECTROPHORESIS APPARATUS 审中-公开
标题翻译：电泳和电泳装置仪器
公开(公告)号：US20120273360A1
公开(公告)日：2012-11-01
申请号：US13518686
申请日：2010-12-21
申请人： Hideki Kinoshita , Yuji Maruo , Yutaka Unuma , Atsunori Hiratsuka , Koji Sakairi , Satonari Akutsu
发明人： Hideki Kinoshita , Yuji Maruo , Yutaka Unuma , Atsunori Hiratsuka , Koji Sakairi , Satonari Akutsu
IPC分类号： C25B9/00 , G01N27/447
CPC分类号： G01N27/44756 , G01N27/44743
摘要： An instrument for electrophoresis includes a sample-separating portion in which a slab sample-separating medium that separates a sample is provided. The instrument for electrophoresis includes a sample-transporting portion that transports a sample-containing medium containing the sample to the sample-separating portion. The instrument for electrophoresis includes a positioning portion that is provided to the sample-separating portion and/or the sample-transporting portion and used for connecting the sample-transporting portion to a predetermined position of the sample-separating portion.
摘要翻译：电子仪器包括样品分离部分，其中提供分离样品的板坯样品分离介质。用于电泳的仪器包括将含有样品的含样本培养基输送到样品分离部分的样品输送部分。电泳仪包括设置在样品分离部分和/或样品输送部分上的定位部分，用于将样品输送部分连接到样品分离部分的预定位置。

10. 发明授权

US07307261B2 Fluorescence detecting apparatus 失效
标题翻译：荧光检测装置
公开(公告)号：US07307261B2
公开(公告)日：2007-12-11
申请号：US11397893
申请日：2006-04-05
申请人： Yuji Maruo
发明人： Yuji Maruo
IPC分类号： G01N21/64
CPC分类号： G01N21/6458 , G01N21/645 , G01N21/6452 , G01N2021/6463
摘要： A fluorescence detecting apparatus includes: a light source for illuminating the surface of detection of a sample with excitation light; a CCD area image sensor for detecting fluorescence emitted from the sample; and a micromirror array device for irradiating excitation light in a 2-dimensional illumination pattern generated by an information processor based on the detected result from the CCD area image sensor. In this configuration, irradiation of weak excitation light on areas that contain large amounts of fluorescent dyes hence would emit strong fluorescence makes it possible to excite reduced amounts of fluorescence and thereby inhibit adverse effects such as blurring or spreading of light of fluorescent wavelengths around the areas as well as to prevent the high-sensitivity fluorescence detecting device from being saturated by excessive amounts of light. On the other hand, irradiation of strong excitation light on areas that contain lower amounts of fluorescent dyes hence would emit weak fluorescence makes it possible to obtain high enough amounts of fluorescence that can be detected by the fluorescence detecting device.
摘要翻译：荧光检测装置包括：用激发光照射样品的检测表面的光源; 用于检测从样品发出的荧光的CCD区域图像传感器; 以及微镜阵列装置，用于基于来自CCD区域图像传感器的检测结果，照射由信息处理器生成的二维照明图案中的激发光。在这种结构中，在含有大量荧光染料的区域上的弱激发光的照射因此会发出强烈的荧光，可以激发减少量的荧光，从而抑制不利影响，例如区域周围的荧光波长的光的模糊或扩展并且防止高灵敏度荧光检测装置被过量的光饱和。另一方面，在含有较少量的荧光染料的区域上的强激发光的照射因此会发出弱荧光使得可以获得足够高的荧光检测量的荧光量。

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