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1. 发明申请

US20110229931A1 Engineering intracellular sialylation pathways 有权
标题翻译：工程细胞内唾液酸化途径
公开(公告)号：US20110229931A1
公开(公告)日：2011-09-22
申请号：US12806292
申请日：2010-08-09
申请人： Donald Jarvis , Michael J. Betenbaugh , Shawn Lawrence , Yuan C. Lee , Timothy A. Coleman
发明人： Donald Jarvis , Michael J. Betenbaugh , Shawn Lawrence , Yuan C. Lee , Timothy A. Coleman
IPC分类号： C12P21/00 , C12N15/85 , C12N15/80 , C12N15/82 , C12P19/30 , C12N5/10 , C07K2/00
CPC分类号： C12P21/005 , C12N15/52
摘要： Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
摘要翻译：提供了在感兴趣的细胞中操纵碳水化合物处理途径的方法。方法是通过使用重组DNA技术和底物进料方法来操纵唾液酸化反应所涉及的多个途径，以使得能够在感兴趣的细胞中产生唾液酸化糖蛋白。这些碳水化合物工程的努力包括实施新的碳水化合物生物测定，检查昆虫细胞系的选择以及使用生物信息学来鉴定关键加工酶的基因序列。组合物包含感兴趣的细胞产生唾液酸化糖蛋白。所述方法和组合物可用于糖蛋白的异源表达。

2. 发明授权

US08546105B2 Engineering intracellular sialylation pathways 有权
公开(公告)号：US08546105B2
公开(公告)日：2013-10-01
申请号：US12806292
申请日：2010-08-09
申请人： Donald Jarvis , Michael J. Betenbaugh , Shawn Lawrence , Yuan C. Lee , Timothy A. Coleman
发明人： Donald Jarvis , Michael J. Betenbaugh , Shawn Lawrence , Yuan C. Lee , Timothy A. Coleman
IPC分类号： C12N15/09 , C12N15/79 , C12N15/866 , C12P19/30
CPC分类号： C12P21/005 , C12N15/52
摘要： Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.

3. 发明授权

US07776565B2 Engineering intracellular sialylation pathways 有权
标题翻译：工程细胞内唾液酸化途径
公开(公告)号：US07776565B2
公开(公告)日：2010-08-17
申请号：US12394479
申请日：2009-02-27
申请人： Michael J. Betenbaugh , Shawn Lawrence , Yuan C. Lee , Timothy A. Coleman
发明人： Michael J. Betenbaugh , Shawn Lawrence , Yuan C. Lee , Timothy A. Coleman
IPC分类号： C12P19/26 , C12P21/00 , C12N15/00
CPC分类号： C12P21/005 , C12N15/52
摘要： Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
摘要翻译：提供了在感兴趣的细胞中操纵碳水化合物处理途径的方法。方法是通过使用重组DNA技术和底物进料方法来操纵唾液酸化反应所涉及的多个途径，以使得能够在感兴趣的细胞中产生唾液酸化糖蛋白。这些碳水化合物工程的努力包括实施新的碳水化合物生物测定，检查昆虫细胞系的选择以及使用生物信息学来鉴定临界加工酶的基因序列。组合物包含感兴趣的细胞产生唾液酸化糖蛋白。所述方法和组合物可用于糖蛋白的异源表达。

4. 发明授权

US06949372B2 Engineering intracellular sialylation pathways 有权
标题翻译：工程细胞内唾液酸化途径
公开(公告)号：US06949372B2
公开(公告)日：2005-09-27
申请号：US09930440
申请日：2001-08-16
申请人： Michael J. Betenbaugh , Shawn Lawrence , Yuan C. Lee , Timothy A. Coleman
发明人： Michael J. Betenbaugh , Shawn Lawrence , Yuan C. Lee , Timothy A. Coleman
IPC分类号： C12N15/52 , C12P21/00 , C12N9/00 , C07H21/04 , C12N9/10
CPC分类号： C12P21/005 , C12N15/52
摘要： Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
摘要翻译：提供了在感兴趣的细胞中操纵碳水化合物处理途径的方法。方法是通过使用重组DNA技术和底物进料方法来操纵唾液酸化反应所涉及的多个途径，以使得能够在感兴趣的细胞中产生唾液酸化糖蛋白。这些碳水化合物工程的努力包括实施新的碳水化合物生物测定，检查昆虫细胞系的选择以及使用生物信息学来鉴定关键加工酶的基因序列。组合物包含感兴趣的细胞产生唾液酸化糖蛋白。所述方法和组合物可用于糖蛋白的异源表达。

5. 发明授权

US5994517A Ligands to enhance cellular uptake of biomolecules 失效
标题翻译：配体增强生物分子的细胞摄取
公开(公告)号：US5994517A
公开(公告)日：1999-11-30
申请号：US755062
申请日：1996-11-22
申请人： Paul O. P. Ts'o , Jon J. Hangeland , Yuan C. Lee
发明人： Paul O. P. Ts'o , Jon J. Hangeland , Yuan C. Lee
IPC分类号： A61K31/00 , A61K31/70 , A61K31/7088 , A61K31/7125 , A61K47/48 , A61K48/00 , A61P1/00 , A61P1/16 , C07H15/18 , C07H21/00 , C07K16/00
CPC分类号： C07H21/00 , A61K47/48092 , A61K47/48276 , C07H15/18
摘要： Oligodeoxynucleoside methylphosphonate neoglycopeptide conjugates and related compounds for tissue specific delivery of biologically stable, nonionic oligodeoxynucleoside analogs into cells.
摘要翻译：寡脱氧核苷甲基膦酸盐新聚糖共轭物和相关化合物，用于组织特异性递送生物稳定的非离子寡脱氧核苷类似物进入细胞。

6. 发明申请

US20080207532A1 Use of Nordihydroguaiaretic Acid Derivatives in the Treatment of Drug Resistant Cancer, Viral and Microbial Infection 审中-公开
标题翻译：使用诺达氢愈创木酚酸衍生物治疗耐药性癌症，病毒和微生物感染
公开(公告)号：US20080207532A1
公开(公告)日：2008-08-28
申请号：US11664875
申请日：2005-10-06
申请人： Ru Chih Huang , Jih Ru Hwu , Ming-Hua Hsu , David E. Mold , Yuan C. Lee , Chih-Chuan Chang
发明人： Ru Chih Huang , Jih Ru Hwu , Ming-Hua Hsu , David E. Mold , Yuan C. Lee , Chih-Chuan Chang
IPC分类号： A61K31/7034 , C12N5/06 , C07H15/04 , C12Q1/02 , A61P35/00 , A61P31/00
CPC分类号： A61K31/192 , A61K31/09 , A61K31/24 , A61K31/337 , A61K31/475 , A61K31/7034 , A61K31/704 , A61K45/06 , C07H15/18 , A61K2300/00
摘要： Compositions and methods for using nordihydroguaiaretic acid (NDGA) derivatives for preventing the expression of MDR-1 gene and the synthesis of PgP protein or reversing multiple drug resistance in cells, and for using NDGA derivatives in combination with additional chemotherapeutic agents to treat drug resistant cancer and infections.
摘要翻译：使用去甲二氢愈创木酸（NDGA）衍生物预防MDR-1基因表达和合成PgP蛋白或逆转细胞中多重耐药性的组合物和方法，以及使用NDGA衍生物与其他化学治疗剂联合治疗耐药性癌症的组合物和方法和感染。

7. 发明授权

US5501841A Connection-type treatment system for micro solution and method of treatment 失效
标题翻译：微溶液连接式处理系统及其处理方法
公开(公告)号：US5501841A
公开(公告)日：1996-03-26
申请号：US282097
申请日：1994-07-27
申请人： Yuan C. Lee , Shinji Inoue , Akinori Tsujimoto , Akimasa Miwa
发明人： Yuan C. Lee , Shinji Inoue , Akinori Tsujimoto , Akimasa Miwa
IPC分类号： B01L3/00 , B01L3/14
CPC分类号： B01L3/565 , B01L3/502 , B01L3/5021 , Y10T436/25375
摘要： A connection type fluid transfer and treatment system apparatus and method for efficiently and continuously executing transfer and treatment of small or micro amounts of sample solutions without substantial transfer loss, which includes a first microsolution reaction microtube having one open end and a second closed end, a second microsolution target microtube having substantially the same shape as the first microtube also having one open end and one closed end, and a connector for connecting together the open ends of the first microtube to the open end of the second tube. The connector includes a foramenous membrane support, which removably receives chemically or biologically treated membranes for applying a predetermined treatment to a solution while passing the sample solution from the first microtube to the second tube. Alternately, a "single use only" connector having disposed therein a membrane pretreated with a quantitative amount of reagent may be used for certain treatment operations. The single use only connector is used once and then discarded. An appropriate color indicator in the connector or membrane would serve to indicate whether the connector had been used. The sample is typically filtered through the membrane by centrifugation. The assembly also includes special adapters for receivingly engaging the dual tube/connector transfer system during centrifugation. The system permits handling microliter quantities of reactive solutions in biochemical analyses, treatments and assays without use of micropipets, without the usual loss of solution. An enzyme microsolution test kit system and method of use comprising one or more micro solution microtubes containing a predetermined quantity of a known, preferably lyophilized, reagent pre-coated on the microtube walls is disclosed.
摘要翻译：一种用于有效且连续地执行小或微量样品溶液的转移和处理而没有实质的转移损失的连接型流体转移和处理系统装置和方法，其包括具有一个开放端和第二封闭端的第一微溶液反应微管，具有与具有一个开放端和一个封闭端的第一微管基本上相同形状的第二微溶液靶微管，以及用于将第一微管的开口端连接到第二管的开口端的连接器。连接器包括孔式膜支撑件，其可移除地接收化学或生物处理的膜，用于在将样品溶液从第一微管传递到第二管的同时对溶液施加预定的处理。或者，可以使用在其中设置有用定量量的试剂预处理的膜的“一次性使用”连接器用于某些处理操作。仅使用一次连接器一次，然后丢弃。连接器或膜中适当的颜色指示器将用于指示连接器是否已被使用。样品通常通过离心过滤通过膜。该组件还包括用于在离心过程中接收双管/连接器传送系统的特殊适配器。该系统允许在生物化学分析，处理和测定中处理微量数量的反应溶液而不使用微量元件，而无需通常的溶液损失。公开了一种酶微溶解测试试剂盒系统和使用方法，其包含一个或多个微溶液微管，其含有预定量的预先涂覆在微管壁上的已知优选冻干的试剂。

8. 发明授权

US5142031A Method for labelling sugars 失效
标题翻译：标签糖的方法
公开(公告)号：US5142031A
公开(公告)日：1992-08-25
申请号：US773325
申请日：1991-10-11
申请人： Yuan C. Lee , Susumu Honda , Kazuaki Kakehi
发明人： Yuan C. Lee , Susumu Honda , Kazuaki Kakehi
IPC分类号： G01N33/53 , C07H1/00 , C07H21/00 , G01N33/532 , G01N33/58 , G01N33/66
CPC分类号： C07H21/00 , C07H1/00 , G01N33/532 , Y10S435/81 , Y10S435/975 , Y10T436/10 , Y10T436/104165 , Y10T436/104998 , Y10T436/105831
摘要： Disclosed herein is a method of labelling a sugar at its reducing end by using 1-(p-methoxyphenyl)-3-methyl-5-pyrazolone, thereby analyzing said sugar with high sensitivity, and to a kit to be used for sugar labelling by this method.

9. 发明申请

US20090226968A1 Engineering Intracellular Sialylation Pathways 有权
标题翻译：工程细胞内唾液酸通路
公开(公告)号：US20090226968A1
公开(公告)日：2009-09-10
申请号：US12394479
申请日：2009-02-27
申请人： Michael J. Betenbaugh , Shawn Lawrence , Yuan C. Lee , Timothy A. Coleman
发明人： Michael J. Betenbaugh , Shawn Lawrence , Yuan C. Lee , Timothy A. Coleman
IPC分类号： C12P21/00 , C12N9/00
CPC分类号： C12P21/005 , C12N15/52
摘要： Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
摘要翻译：提供了在感兴趣的细胞中操纵碳水化合物处理途径的方法。方法是通过使用重组DNA技术和底物进料方法来操纵唾液酸化反应所涉及的多个途径，以使得能够在感兴趣的细胞中产生唾液酸化糖蛋白。这些碳水化合物工程的努力包括实施新的碳水化合物生物测定，检查昆虫细胞系的选择以及使用生物信息学来鉴定关键加工酶的基因序列。组合物包含感兴趣的细胞产生唾液酸化糖蛋白。所述方法和组合物可用于糖蛋白的异源表达。

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