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1. 发明申请

US20070134806A1 Quantitation method using isotope labeled internal standard substance, analysis system for executing the quantitation method, and program for the analysis 失效
标题翻译：使用同位素标记的内标物质的定量方法，用于执行定量方法的分析系统和分析程序
公开(公告)号：US20070134806A1
公开(公告)日：2007-06-14
申请号：US10579780
申请日：2004-11-22
申请人： Yoshiya Oda , Yasushi Ishihama , Tsuyoshi Tabata
发明人： Yoshiya Oda , Yasushi Ishihama , Tsuyoshi Tabata
IPC分类号： G01N24/00
CPC分类号： G01N33/6848 , Y10T436/10 , Y10T436/104165 , Y10T436/104998 , Y10T436/105831 , Y10T436/24
摘要： Problem: An object of the present invention is to quantitate with good accuracy, furthermore, quantitate absolutely, one or a plurality of biological molecules in a sample such as a tissue, a biological fluid, a cell, a cell organ or protein complex. Solution: By adding a metabolically isotope labeled biological molecule as an internal standard substance and measuring with a mass spectrometer, quantitating with good accuracy one or a plurality of target molecules in a sample has become possible. In addition, by performing waveform separation processing during mass analysis, a highly accurate quantitative analysis method of mass analysis is provided.
摘要翻译：问题：本发明的目的是准确定量，此外，在样品如组织，生物流体，细胞，细胞器官或蛋白质复合物中绝对地定量分析一种或多种生物分子。解决方案：通过添加代谢同位素标记的生物分子作为内标物质并用质谱仪进行测量，以高精度定量，样品中的一个或多个目标分子成为可能。此外，通过在质量分析中进行波形分离处理，提供了高精度的质量分析的定量分析方法。

2. 发明授权

US06929741B2 Column packaging for chromatography 有权
标题翻译：色谱柱色谱柱包装
公开(公告)号：US06929741B2
公开(公告)日：2005-08-16
申请号：US10181779
申请日：2001-02-09
申请人： Eiichi Yamamoto , Kaoru Murata , Yasushi Ishihama , Naoki Asakawa
发明人： Eiichi Yamamoto , Kaoru Murata , Yasushi Ishihama , Naoki Asakawa
IPC分类号： B01J20/32 , G01N30/08 , B01D15/08
CPC分类号： B01J20/286 , B01J20/3244 , B01J20/327 , B01J20/3272 , B01J20/3285 , B01J2220/54 , B01J2220/58 , G01N2030/085 , Y10T428/31663
摘要： The present invention provides a packing for chromatography. Specifically, it provides a packing for chromatography in which a hydrophilic polymeric material is bound to the surface of a porous carrier, and a carbon chain is chemically bound inside the pore. By the use of the packing according to the present invention for analysis of low molecular weight compounds in a biological sample, a pretreatment such as removal of proteins etc. becomes unnecessary, and the biological sample can be directly analyzed.
摘要翻译：本发明提供了一种色谱填料。具体地说，它提供了用于色谱法的填料，其中亲水性聚合物材料结合到多孔载体的表面，并且碳链化学结合在孔内。通过使用根据本发明的包装用于分析生物样品中的低分子量化合物，不需要诸如去除蛋白质等的预处理，并且可以直接分析生物样品。

3. 发明授权

US06586065B1 Capillary having coated inner wall 有权
标题翻译：毛细管涂有内壁
公开(公告)号：US06586065B1
公开(公告)日：2003-07-01
申请号：US09319043
申请日：1999-06-01
申请人： Hiroyuki Katayama , Yasushi Ishihama
发明人： Hiroyuki Katayama , Yasushi Ishihama
IPC分类号： G01N2726
CPC分类号： G01N27/44704 , Y10T428/1321 , Y10T428/139 , Y10T428/31536
摘要： It is to provide a capillary having adsorbed a polymer. A capillary having absorbed on an inner wall of the capillary, an ionic polymer having positive electric charge and an ionic polymer having negative electric charge alternately, and a process for producing the same.
摘要翻译：本发明提供吸附了聚合物的毛细管。毛细管吸收在毛细管内壁上的毛细管，具有正电荷的离子聚合物和交替负电荷的离子聚合物及其制造方法。

4. 发明授权

US06413698B1 Photohardenable resin composition providing heat-resistant photohardened product 有权
标题翻译：提供耐热光硬化产品的可光硬化树脂组合物
公开(公告)号：US06413698B1
公开(公告)日：2002-07-02
申请号：US09478462
申请日：2000-01-06
申请人： Yorikazu Tamura , Tsuneo Hagiwara , Makoto Ohtake , Minoru Shinoda , Toshiharu Suzuki , Tatsuhiko Ozaki , Yasushi Ishihama , Takayuki Nakamura
发明人： Yorikazu Tamura , Tsuneo Hagiwara , Makoto Ohtake , Minoru Shinoda , Toshiharu Suzuki , Tatsuhiko Ozaki , Yasushi Ishihama , Takayuki Nakamura
IPC分类号： G03F7028
CPC分类号： G03F7/0037 , B33Y70/00 , C08G18/675 , C08L75/16 , G03F7/027
摘要： A photohardenable resin composition comprising (A) a urethanated acrylic compound represented by formula (I): (A)a—C—(R)4−a wherein R is H or alkyl; a is 3 or 4; and A represents a group of formula (II): wherein D represents —CH2CH2O—, —CH2CH(CH3)O—, —CH2CH2COO— or —(CH2)6COO—; E represents —CH2CH2O—, —CH2CH(CH3)O—, —COCH2CH2O— or —CO(CH2)6O—; G represents a diol or triol residual group; J represents a divalent or trivalent and substituted or unsubstituted hydrocarbon group; L represents —(CH2CH2O)h— (h: integer of 1 to 4), —(CH2CH(CH3)O)j— (j: integer of 1 to 4), or —(CH2CH2O)k—(CH2CH(CH3)O)m— (k, m: integer of 1 to 3 (k+m=2, 3 or 4); b, c, and d are each 0 or 1 (b+c+d=1, 2 or 3); e is 0, 1 or 2; f is 0 or 1 (e+f=1 or 2); and g is 1 or 2, and (B) a radical polymerizable compound at a (A) : (B) ratio of 80:20 to 10:90 by weight.
摘要翻译：一种可光硬化树脂组合物，其包含（A）由式（I）表示的氨基甲酸酯化丙烯酸化合物：其中R是H或烷基; a是3或4; 并且A表示式（II）的基团：其中D表示-CH 2 CH 2 O-，-CH 2 CH（CH 3）O - ， - CH 2 CH 2 COO-或 - （CH 2）6 COO-; E表示-CH 2 CH 2 O-，-CH 2 CH（CH 3）O-，-COCH 2 CH 2 O-或-CO（CH 2）6 O-; G表示二醇或三醇残基; J表示二价或三价且取代或未取代的烃基; L表示 - （CH 2 CH 2 O）h - （h：1〜4的整数）， - （CH 2 CH（CH 3）O）j-（j：1〜4的整数）或 - （CH 2 CH 2 O）k-（CH 2 CH O）m-（k，m：1〜3的整数（k + m = 2,3或4）; b，c和d分别为0或1（b + c + d = 1,2或3） ; e为0,1或2; f为0或1（e + f = 1或2）; g为1或2，（B）自由基聚合性化合物的（A）：（B） 80:20至10:90（重量）

5. 发明授权

US06581442B1 Splitted tubing apparatus for gradient high performance liquid chromatography 失效
标题翻译：用于梯度高效液相色谱的分流管装置
公开(公告)号：US06581442B1
公开(公告)日：2003-06-24
申请号：US09979116
申请日：2001-11-19
申请人： Kaoru Murata , Yasushi Ishihama , Nariyasu Mano , Naoki Asakawa
发明人： Kaoru Murata , Yasushi Ishihama , Nariyasu Mano , Naoki Asakawa
IPC分类号： G01N100
CPC分类号： B01F5/064 , G01N30/34 , G01N30/6095 , G01N2030/347 , G01N30/10
摘要： The present invention provides a device capable of simply formulating an arbitrary gradient pattern in a low flow gradient high performance liquid chromatography. That is, the present invention provides a split tubing apparatus for a gradient high performance liquid chromatography, which comprises a solvent inlet part, a split tubing part split into two or more plural tubes, and a solvent outlet part.
摘要翻译：本发明提供能够简单地在低流动梯度高效液相色谱中配制任意梯度图案的装置。也就是说，本发明提供了一种用于梯度高效液相色谱的分离管装置，其包括溶剂入口部分，分成两个或更多个管的裂解管部分和溶剂出口部分。

6. 发明申请

US20110083962A1 ADSORPTION PREVENTION METHOD OF INNER-WALL-COATED CAPILLARY FOR CAPILLARY ELECTROPHORESIS, INNER-WALL-COATED CAPILLARY FOR CAPILLARY ELECTROPHORESIS, MANUFACTURING METHOD THEREOF, AND METHOD FOR SIMULTANEOUS ANALYSIS OF PHOSPHORYLATED COMPOUND AND ANION BY CAPILLARY ELECTROPHORESIS 审中-公开
标题翻译：用于毛细管电泳的内壁包裹毛细管的吸收预防方法，用于毛细管电泳的内壁包裹毛细管的制造方法，以及通过毛细管电泳同时分析磷酸化合物和阴离子的方法
公开(公告)号：US20110083962A1
公开(公告)日：2011-04-14
申请号：US12993343
申请日：2009-05-22
申请人： Yasushi Ishihama , Maria R. N. Monton
发明人： Yasushi Ishihama , Maria R. N. Monton
IPC分类号： G01N27/447 , B05D7/22
CPC分类号： G01N27/44704 , B01J20/28097 , B01J20/283 , B01J20/3272 , B01J20/3278 , B01J2220/84 , B01J2220/86 , G01N30/6078
摘要： A inner-wall-coated capillary in which a zwitterionic polymer including a phosphate group including, for example, a phosphorylcholine group, is fixed on a wall surface including, for example, silanol by ionic interaction, is obtained by flowing a polymer solution including a phospholipid polymer combining, for example, MPC and BMA or MPC and SMA, through a capillary that includes silanol on the wall surface, for example. Consequently, adsorption of phosphorylated compounds can be prevented simply and highly durably.
摘要翻译：通过使含有例如磷酸胆碱基团的磷酸酯基的两性离子聚合物通过离子相互作用固定在包括例如硅烷醇的壁表面上的内壁涂布毛细管，例如通过在壁表面上包括硅烷醇的毛细管结合例如MPC和BMA或MPC和SMA的磷脂聚合物。因此，可以简单耐久地防止磷酸化化合物的吸附。

7. 发明授权

US07910372B2 Absolute quantitation of protein contents based on exponentially modified protein abundance index by mass spectrometry 有权
标题翻译：基于指数变化的蛋白质丰度指数通过质谱法对蛋白质含量进行绝对定量
公开(公告)号：US07910372B2
公开(公告)日：2011-03-22
申请号：US11658592
申请日：2005-07-04
申请人： Yasushi Ishihama
发明人： Yasushi Ishihama
IPC分类号： G01N33/00 , G01N33/48
CPC分类号： G01N33/6803 , G01N33/6848
摘要： The present inventor has established protein abundance index (PAI, π) to determine the protein contents in a protein mixture solution using nanoLC-MSMS data. Digested peptides were analyzed by nanoLC-MS/MS and the obtained results were applied to a Mascot protein identification algorism based on tandem mass spectra. For absolute quantitation, PAI was converted to exponentially modified PAI (EMPAI, m π), which is proportional to protein contents in the protein mixture. EMPAI was successfully applied to comprehensive protein expression analysis and performed a comparison study between gene and protein expression in an HCT116 human cancer cells. Accordingly, the present invention provides a method and a computer program for quantifying the protein contents based on the protein abundance index.
摘要翻译：本发明人利用nanoLC-MSMS数据建立了蛋白质丰度指数（PAI，pgr），以确定蛋白质混合溶液中的蛋白质含量。通过纳米LC-MS / MS分析消化肽，将得到的结果应用于基于串联质谱的吉祥物蛋白鉴定算法。为了绝对定量，PAI被转化为指数变化的PAI（EMPAI，m＆pgr），其与蛋白质混合物中的蛋白质含量成比例。 EMPAI成功应用于综合蛋白表达分析，并在HCT116人类癌细胞中进行基因与蛋白质表达的比较研究。因此，本发明提供了一种用于基于蛋白质丰度指数量化蛋白质含量的方法和计算机程序。

8. 发明申请

US20110022326A1 Quantitation Method Using Isotope Labeled Internal Standard Substance, Analysis System for Executing the Quantitation Method, and Program for the Analysis 审中-公开
标题翻译：使用同位素标记的内标物质的定量方法，执行定量方法的分析系统和分析程序
公开(公告)号：US20110022326A1
公开(公告)日：2011-01-27
申请号：US12787712
申请日：2010-05-26
申请人： Yoshiya ODA , Yasushi Ishihama , Tsuyoshi Tabata
发明人： Yoshiya ODA , Yasushi Ishihama , Tsuyoshi Tabata
IPC分类号： G06F19/00 , C12N5/07 , C12Q1/02 , C07C229/08
CPC分类号： G01N33/6848 , Y10T436/10 , Y10T436/104165 , Y10T436/104998 , Y10T436/105831 , Y10T436/24
摘要： Problem: An object of the present invention is to quantitate with good accuracy, furthermore, quantitate absolutely, one or a plurality of biological molecules in a sample such as a tissue, a biological fluid, a cell, a cell organ or protein complex.Solution: By adding a metabolically isotope labeled biological molecule as an internal standard substance and measuring with a mass spectrometer, quantitating with good accuracy one or a plurality of target molecules in a sample has become possible. In addition, by performing waveform separation processing during mass analysis, a highly accurate quantitative analysis method of mass analysis is provided.
摘要翻译：问题：本发明的目的是准确定量，此外，在样品如组织，生物流体，细胞，细胞器官或蛋白质复合物中绝对地定量分析一种或多种生物分子。解决方案：通过添加代谢同位素标记的生物分子作为内标物质并用质谱仪进行测量，以高精度定量，样品中的一个或多个目标分子成为可能。此外，通过在质量分析中进行波形分离处理，提供了高精度的质量分析的定量分析方法。

9. 发明授权

US07759130B2 Mass spectrometric quantitation method for biomolecules based on metabolically labeled internal standards 失效
标题翻译：基于代谢标记内标的生物分子的质谱定量方法
公开(公告)号：US07759130B2
公开(公告)日：2010-07-20
申请号：US10579780
申请日：2004-11-22
申请人： Yoshiya Oda , Yasushi Ishihama , Tsuyoshi Tabata
发明人： Yoshiya Oda , Yasushi Ishihama , Tsuyoshi Tabata
IPC分类号： G01N24/00
CPC分类号： G01N33/6848 , Y10T436/10 , Y10T436/104165 , Y10T436/104998 , Y10T436/105831 , Y10T436/24
摘要： An object of the present invention is to quantitate with good accuracy, furthermore, quantitate absolutely, one or a plurality of biological molecules in a sample such as a tissue, a biological fluid, a cell, a cell organ or protein complex.By adding a metabolically isotope labeled biological molecule as an internal standard substance and measuring with a mass spectrometer, quantitating with good accuracy one or a plurality of target molecules in a sample has become possible. In addition, by performing waveform separation processing during mass analysis, a highly accurate quantitative analysis method of mass analysis is provided.
摘要翻译：本发明的目的是准确定量，另外，在样品如组织，生物流体，细胞，细胞器官或蛋白质复合物中绝对地定量分析一种或多种生物分子。通过添加代谢同位素标记的生物分子作为内标物质并用质谱仪进行测量，以高精度定量，样品中的一个或多个靶分子已成为可能。此外，通过在质量分析中进行波形分离处理，提供了高精度的质量分析的定量分析方法。

10. 发明申请

US20100012832A1 METHOD OF SEPARATING PHOSPHORYLATED PEPTIDE OR PHOSPHORYLATED PROTEIN 审中-公开
标题翻译：分离磷酸化肽或磷酸化蛋白的方法
公开(公告)号：US20100012832A1
公开(公告)日：2010-01-21
申请号：US12374966
申请日：2007-08-09
申请人： Yasushi Ishihama
发明人： Yasushi Ishihama
IPC分类号： B01D59/44 , C07K1/16 , C01G23/04
CPC分类号： C07K1/22 , B01D15/325 , B01D15/3828 , B01D2015/3838 , B01J20/0211 , B01J20/06 , B01J20/08 , B01J20/282 , B01J20/283 , B01J2220/54 , B01J2220/82 , C07K1/20 , C07K1/36 , G01N30/7233 , G01N2030/8831
摘要： According to the present invention, phosphorylated peptides and/or phosphorylated proteins are specifically separated. A sample containing a phosphorylated peptide and/or a phosphorylated protein is supplied to a separation unit filled with a metal oxide in the presence of an aliphatic hydroxycarboxylic acid. Upon separation of a phosphorylated peptide and/or a phosphorylated peptide with the use of a separation unit filled with a metal oxide, adsorption of carboxylic acid to an acidic peptide can be prevented in the presence of aliphatic hydroxycarboxylic acid. In addition, aliphatic hydroxycarboxylic acid does not inhibit adsorption of a phosphorylated peptide and a phosphoric acid group in the phosphorylated peptide to a metal oxide.
摘要翻译：根据本发明，磷酸化肽和/或磷酸化蛋白被特异性分离。将含有磷酸化肽和/或磷酸化蛋白质的样品在脂肪族羟基羧酸的存在下供给到填充有金属氧化物的分离单元。在使用填充有金属氧化物的分离单元分离磷酸化肽和/或磷酸化肽时，可以在脂族羟基羧酸的存在下防止羧酸向酸性肽的吸附。此外，脂肪族羟基羧酸不抑制磷酸化肽中的磷酸化肽和磷酸基团对金属氧化物的吸附。

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