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1. 发明授权

US09315874B2 Bacillus subtilis mutant strain and a fermentation method for producing acetoin using this organism 有权
标题翻译：枯草芽孢杆菌突变菌株和使用该生物体产生乙偶姻的发酵方法
公开(公告)号：US09315874B2
公开(公告)日：2016-04-19
申请号：US11970573
申请日：2008-01-08
申请人： Jianjun Liu , Xiangying Zhao , Jiaxiang Zhang , Yanjun Tian , Yanlei Han , Li Han
发明人： Jianjun Liu , Xiangying Zhao , Jiaxiang Zhang , Yanjun Tian , Yanlei Han , Li Han
IPC分类号： C12N1/20 , C12R1/125 , C12P7/26
CPC分类号： C12R1/125 , C12P7/26
摘要： The present invention provides a mutant strain of Bacillus subtilis SFA-H31 and includes an optimized method for producing of acetoin using this strain. The advantage of this method to produce acetoin has been defined by its high-yield without mixture with diacetyl or 2,3-butanediol, both of which are usually accompanied with acetoin in other strain. This Bacillus subtilis mutant strain has been deposited in China General Microbiological Culture Collection (CGMCC) and the accession number is CGMCC No. 1869, in which the process of fermentation for acetoin production is composed of (1) strain activation, (2) seed culture, (3) fermentation, (4) quantification of substrates and products. Based on current method, the concentration of acetoin in the ferment broth can reach to 35-55 g/L and the conversion rate of glucose to acetoin is in the range of 40-50%.
摘要翻译：本发明提供了枯草芽孢杆菌SFA-H31的突变菌株，并且包括使用该菌株产生乙偶姻的优化方法。该方法生产乙偶姻的优点是通过其高产率而不与二乙酰基或2,3-丁二醇混合来定义，两者通常在其它菌株中伴有乙偶姻。该枯草芽孢杆菌突变菌株已经保存在中国通用微生物培养物保藏中心（CGMCC），登录号为CGMCC No.1869，其中乙偶姻生产发酵过程由（1）菌株活化，（2）种子培养，（3）发酵，（4）定量底物和产品。基于目前的方法，发酵液中乙偶姻的浓度可达35-55g / L，葡萄糖转化成乙偶姻的转化率在40-50％的范围内。

2. 发明申请

US20080182306A1 BACILLUS SUBTILIS MUTANT STRAIN AND A FERMENTATION METHOD FOR PRODUCING ACETOIN USING THIS ORGANISM 有权
标题翻译：巴豆芽孢杆菌突变体菌株和使用该有机物生产乙草胺的发酵方法
公开(公告)号：US20080182306A1
公开(公告)日：2008-07-31
申请号：US11970573
申请日：2008-01-08
申请人： Jianjun LIU , Xiangying ZHAO , Jiaxiang ZHANG , Yanjun TIAN , Yanlei HAN , Li HAN
发明人： Jianjun LIU , Xiangying ZHAO , Jiaxiang ZHANG , Yanjun TIAN , Yanlei HAN , Li HAN
IPC分类号： C12P7/26 , C12N1/20
CPC分类号： C12R1/125 , C12P7/26
摘要： The present invention provides a mutant strain of Bacillus subtilis SFA-H31 and includes an optimized method for producing of acetoin using this strain. The advantage of this method to produce acetoin has been defined by its high-yield without mixture with diacetyl or 2,3 butanedinol, both of which are usually accompanied with acetoin in other strain. This Bacillus subtilis mutant strain has been deposited in China General Microbilogical Culture Collection (CGMCC) and the accession number is CGMCC No. 1869, in which the process of fermentation for acetoin production is composed of (1) strain activation, (2) seed culture, (3) fermentation, (4) quantification of substrates and products. Based on current method, the concentration of acetoin in the ferment broth can reach to 35-55 g/L and the conversion rate of glucose to acetoin is in the range of 40-50%.
摘要翻译：本发明提供了枯草芽孢杆菌SFA-H31的突变菌株，并且包括使用该菌株产生乙偶姻的优化方法。该方法产生乙偶姻的优点是通过其高产率而不与二乙酰基或2,3-丁二醇混合来定义，二者通常在其它菌株中伴随着乙酰丁胺。该枯草芽孢杆菌突变菌株已经存放于中国通用微生物培养物保藏中心（CGMCC），登录号为CGMCC No.1869，其中乙偶姻生产发酵过程由（1）菌株活化，（2）种子培养，（3）发酵，（4）定量底物和产品。基于目前的方法，发酵液中乙偶姻的浓度可达35-55g / L，葡萄糖转化成乙偶姻的转化率在40-50％的范围内。

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