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1. 发明申请

WO2008013874A1 METHODS FOR ASSAYING PERCENTAGE OF GLYCATED HEMOGLOBIN 审中-公开
标题翻译：测定糖化血红蛋白百分比的方法
公开(公告)号：WO2008013874A1
公开(公告)日：2008-01-31
申请号：PCT/US2007/016774
申请日：2007-07-25
申请人： GENERAL ATOMICS , YUAN, Chong-Sheng , LIU, Limin , DATTA, Abhijit
发明人： YUAN, Chong-Sheng , LIU, Limin , DATTA, Abhijit
IPC分类号： G01N33/72
CPC分类号： G01N33/723 , Y10T436/104998 , Y10T436/105831 , Y10T436/107497 , Y10T436/25375
摘要： The invention provides enzymatic methods for direct determination of percentage of glycated hemoglobin in blood samples without the need of a separated measurement of total hemoglobin content in blood samples. The methods utilizes one or two different types of oxidizing agents which selectively oxidize low-molecular weight reducing substances and high-molecular weight (mainly hemoglobin) reducing substances in blood samples, coupled with enzymatic reactions catalyzed by proteases, fructosyl amino acid oxidase, and peroxidase. The invention provides kits for performing the methods of the invention.
摘要翻译：本发明提供了用于直接测定血液样品中糖化血红蛋白百分比的酶法，而不需要分离测量血液样品中的总血红蛋白含量。该方法使用一种或两种不同类型的氧化剂，其选择性地氧化血液样品中的低分子量还原物质和高分子量（主要是血红蛋白）还原物质，加上由蛋白酶，果糖基氨基酸氧化酶和过氧化物酶催化的酶反应。本发明提供了用于实施本发明方法的试剂盒。

2. 发明申请

WO2009089106A1 METHODS AND COMPOSITIONS FOR ASSAYING HOMOCYSTEINE 审中-公开
标题翻译：用于测定半胱氨酸的方法和组合物
公开(公告)号：WO2009089106A1
公开(公告)日：2009-07-16
申请号：PCT/US2009/030041
申请日：2009-01-02
申请人： GENERAL ATOMICS , YUAN, Chong-Sheng , DATTA, Abhijit , DOU, Chao
发明人： YUAN, Chong-Sheng , DATTA, Abhijit , DOU, Chao
IPC分类号： C12Q1/00
CPC分类号： C12Q1/34 , C12Q1/48 , G01N33/6815 , G01N2333/91011
摘要： This invention relates generally to the field of homocysteine detection. In particular, the invention provides a method for determining homocysteine presence or concentration in samples, which method comprises: contacting a sample containing or suspected of containing Hcy with a Hcy co-substrate and a Hcy converting enzyme in a Hcy conversion reaction to form a Hcy conversion product and a Hcy co-substrate conversion product; and assessing the Hcy co-substrate conversion product to determine the presence, absence and/or amount of the Hcy in the sample. The Hcy co-substrate conversion product may be assessed directly, or it may be assessed by further conversion of the Hcy co-substrate conversion product into another material by the action of one or more additional enzymes. A kit for assaying homocysteine based on the same principle is also provided.
摘要翻译：本发明一般涉及同型半胱氨酸检测领域。特别地，本发明提供了一种测定样品中同型半胱氨酸存在或浓度的方法，该方法包括：在Hcy转化反应中使包含或怀疑含有Hcy的样品与Hcy共底物和Hcy转化酶接触以形成Hcy 转化产物和Hcy共底物转化产物; 并评估Hcy共底物转化产物以确定样品中Hcy的存在，不存在和/或量。可以直接评估Hcy共底物转化产物，或者可以通过一种或多种另外的酶的作用将Hcy共底物转化产物进一步转化成另一种物质来评估。还提供了用于基于相同原理测定同型半胱氨酸的试剂盒。

3. 发明申请

WO2006065967A3 ALLOSTERIC ENZYME COUPLED IMMUNOASSAY (AECIA) 审中-公开
标题翻译：异体酶联合免疫（AECIA）
公开(公告)号：WO2006065967A3
公开(公告)日：2006-11-16
申请号：PCT/US2005045374
申请日：2005-12-15
申请人： GEN ATOMICS , YUAN CHONG-SHENG
发明人： YUAN CHONG-SHENG
IPC分类号： C12Q1/48 , G01N33/58 , G01N33/78
CPC分类号： G01N33/78 , C12Q1/485 , G01N33/542 , G01N33/54393 , G01N33/581 , G01N2333/91215
摘要： The present invention is directed to methods and compositions for an allosteric enzyme coupled assay, and preferably to an allosteric enzyme coupled immunoassay (AECIA). The assay uses an allosteric enzyme to generate a readout signal. The assay is based on the competition between an analyte in a sample and an analyte or analyte analog conjugated to an allosteric regulator with a specific binding reagent for the analyte. In the absence of any analyte in the sample, an analyte or an analyte analog conjugated to an allosteric regulator binds to the specific binding reagent and such binding prevents or reduces the allosteric regulator's regulation, e.g., activation, on the allosteric enzyme. An analyte, if present in the sample, competes with the analyte or analyte analog conjugated to the allosteric regulator for binding with the specific binding reagent, reduces or prevents binding of the specific binding reagent to the analyte or analyte analog conjugated to the allosteric regulator, leading to increased regulation, e.g., activation, of the enzyme. 1-substituted-(3-D-fructofuranose 2, 6bisphosphate compounds and conjugates comprising the same are provided. Kits comprising the conjugates, and methods using the conjugates for assaying an analyte are further provided.
摘要翻译：本发明涉及用于变构酶偶联测定法的方法和组合物，并且优选涉及变构酶偶联免疫测定法（AECIA）。该测定使用变构酶来产生读出信号。该测定基于样品中的分析物与与变构调节剂缀合的分析物或分析物类似物与分析物的特定结合试剂之间的竞争。在样品中不存在任何分析物的情况下，与变构调节剂缀合的分析物或分析物类似物与特异性结合试剂结合，并且此类结合防止或减少变构调节剂对变构酶的调节，例如激活。分析物如果存在于样品中，则与缀合至变构调节剂的分析物或分析物类似物竞争与特异性结合试剂结合，减少或防止特异性结合试剂与缀合至变构调节剂的分析物或分析物类似物结合，导致酶的调节增加，例如激活。提供了1-取代的（3-D-呋喃果糖2,6-二磷酸酯化合物和包含它们的缀合物。还提供了包含缀合物的试剂盒和使用缀合物用于分析分析物的方法。

4. 发明申请

WO2006044633A1 ENZYME CYCLING BASED ASSAYS FOR ALPHA-METHYLACYL-CoA RACEMASE 审中-公开
标题翻译：用于ALPHA-METHYLACYL-CoA RACEMASE的基于酶的循环测定
公开(公告)号：WO2006044633A1
公开(公告)日：2006-04-27
申请号：PCT/US2005/036983
申请日：2005-10-14
申请人： GENERAL ATOMICS , YUAN, Chong-Sheng
发明人： YUAN, Chong-Sheng
IPC分类号： C12Q1/26 , C12Q1/00 , G01N33/53
CPC分类号： C12Q1/533 , G01N33/574
摘要： The present invention provides a method for assaying alpha-methylacyl-CoA racemase activity. In the assay, a sample containing an alpha-methylacyl-CoA racemase or suspected of containing an alpha-methylacyl-CoA racemase is contacted with (2R)-2-methylacyl-CoA. If alpha-methylacyl-CoA racemase is present in the sample (2R)-2-methylacyl-CoA is converted into (2S)-methylacyl-CoA. The method then utilizes a cycling reaction system between (2S)-methylacyl-CoA and trans-2,3-dehydroacyl-CoA to generate a detectable signal that corresponds to the alpha-methylacyl-CoA racemase activity. Kits for assaying alpha-methylacyl-CoA racemase based on the same principle are also provided.
摘要翻译：本发明提供了测定α-甲酰基-CoA消旋酶活性的方法。在该测定中，含有α-甲基酰基-CoA消旋酶或怀疑含有α-甲酰基-CoA消旋酶的样品与（2R）-2-甲酰基-CoA接触。如果α-甲酰基-CoA消旋酶存在于样品（2R）中，则将-2-甲酰基-CoA转化为（2S） - 甲酰基-CoA。该方法然后利用（2S） - 甲基酰基-CoA和反式-2,3-脱氢酰基-CoA之间的循环反应体系产生对应于α-甲酰基-CoA消旋酶活性的可检测信号。还提供了基于相同原理测定α-甲基酰基-CoA消旋酶的试剂盒

5. 发明申请

WO2006044573A2 REVERSIBLE INHIBITORS OF S-ADENOSYL-L-HOMOCYSTEINE HYDROLASE AND USES THEREOF 审中-公开
标题翻译： S-腺苷-L-高血糖素水解酶的可逆抑制剂及其应用
公开(公告)号：WO2006044573A2
公开(公告)日：2006-04-27
申请号：PCT/US2005036890
申请日：2005-10-13
申请人： GEN ATOMICS , YUAN CHONG-SHENG
发明人： YUAN CHONG-SHENG
IPC分类号： A61K31/52 , A61K31/4745 , A61K45/06 , C07D471/02 , C07D473/14 , C07D473/34 , G06F19/00
CPC分类号： C07D471/04 , A61K31/52 , A61K45/06 , C07D473/34 , G06F19/16 , G06F19/18 , A61K2300/00
摘要： The present invention provides compositions and methods for reversibly inhibiting Sadenosyl-L-homocysteine (SAH) hydrolase. The compounds of the present invention can be used in combination with an anti-hemorrhagic viral infection agent, an immunosuppressant, a homocysteine lowering agent, or an anti-neoplasm agent. The compositions and methods of the present invention can be used for the prevention and treatment of hemorrhagic virus infection, autoimmune diseases, autograft rejection, neoplasm, hyperhomocysteineuria, cardiovascular disease, stroke, Alzheimer's disease, or diabetes.
摘要翻译：本发明提供用于可逆性抑制Sadenosyl-L-高半胱氨酸（SAH）水解酶的组合物和方法。本发明的化合物可以与抗出血性病毒感染剂，免疫抑制剂，同型半胱氨酸降低剂或抗肿瘤剂组合使用。本发明的组合物和方法可用于预防和治疗出血性病毒感染，自身免疫性疾病，自体移植排斥，肿瘤，高同型半胱氨酸尿症，心血管疾病，中风，阿尔茨海默氏病或糖尿病。

6. 发明申请

WO2006020762A1 METHODS AND KITS FOR ASSAYING MYELOPEROXIDASE BY MEANS OF ENZYMATIC GLYCOLATE-GLYOXYLATE CYCLING REACTIONS 审中-公开
标题翻译：通过醋酸甘油酯 - 葡萄糖酸酯循环反应测定甲氧氯丙酸酶的方法和试剂盒
公开(公告)号：WO2006020762A1
公开(公告)日：2006-02-23
申请号：PCT/US2005/028540
申请日：2005-08-10
申请人： GENERAL ATOMICS , YUAN, Chong-Sheng , DOU, Chao
发明人： YUAN, Chong-Sheng , DOU, Chao
IPC分类号： C12Q1/28
CPC分类号： C12Q1/28 , C12Q1/26 , C12Q1/32
摘要： The present invention provides a method for assaying myeloperoxidase activity. In the assay, a sample containing myeloperoxidase or suspected of containing myeloperoxidase is contacted with substrate including serine, hydrogen peroxide, and a halide. If myeloperoxidase is present in the sample, serine is converted into glycolaldehyde, which is further converted into glycolate by a glycoaldehyde converting enzyme (e.g. glycolaldehyde dehydrogenase) (1.2.1.21). The method then utilizes a cycling reaction system between glycolate and glyoxylate to generate a detectable signal that corresponds to the myeloperoxidase activity. Kits for assaying myeloperoxidases based on the same principle are also provided.
摘要翻译：本发明提供了测定髓过氧化物酶活性的方法。在测定中，含有髓过氧化物酶或怀疑含有髓过氧化物酶的样品与包含丝氨酸，过氧化氢和卤化物的底物接触。如果样品中存在髓过氧化物酶，则将丝氨酸转化为乙二醇醛，通过乙醛醛转化酶（例如乙二醇脱氢酶）（1.2.1.21）进一步转化成乙醇酸。然后该方法利用乙醇酸和乙醛酸之间的循环反应系统产生对应于髓过氧化物酶活性的可检测信号。还提供了基于相同原理测定髓过氧化物酶的试剂盒。

7. 发明申请

WO0102600A9 DETECTION OF ANALYTES USING ATTENUATED ENZYMES 审中-公开
标题翻译：用衰减酶检测分析
公开(公告)号：WO0102600A9
公开(公告)日：2002-07-25
申请号：PCT/US0018057
申请日：2000-06-30
申请人： GEN ATOMICS , YUAN CHONG-SHENG
发明人： YUAN CHONG-SHENG
IPC分类号： C12Q1/25 , C12Q1/34 , G01N33/573 , G01N33/84 , C12Q1/37 , G01N33/68
CPC分类号： G01N33/573 , C12Q1/25 , C12Q1/34 , G01N33/84
摘要： Compositions and methods for assaying analytes, preferably, small molecule analytes are provided. Assay methods employ, in place of antibodies or molecules that bind to target analytes or substrates, modified enzymes, called substrate trapping enzymes. These modified enzymes retain binding affinity or have enhanced binding affinity for a target substrate or analyte, but have attenuated catalytic activity with respect to that substrate or analyte. The modified enzymes are provided. In particular, mutant S-adenosylhomocysteine (SAH) hydrolases, substantially retaining binding affinity or having enhanced binding affinity for homocysteine or S-adenosylhomocysteine but having attenuated catalytic activity, are provided. Conjugates of the modified enzymes and a facilitating agent, such as agents that aid in purification or linkage to a solid support are also provided.
摘要翻译：提供了用于测定分析物，优选小分子分析物的组合物和方法。测定方法使用代替与目标分析物或底物结合的抗体或分子的修饰酶，称为底物捕获酶。这些修饰的酶保留结合亲和力或对目标底物或分析物具有增强的结合亲和力，但相对于该底物或分析物具有减弱的催化活性。提供修饰的酶。具体而言，提供了基本上保持结合亲和力或对同型半胱氨酸或S-腺苷高半胱氨酸具有增强的结合亲和力但具有减弱的催化活性的突变体S-腺苷高半胱氨酸（SAH）水解酶。还提供了修饰酶和促进剂的缀合物，例如帮助纯化或连接至固体支持物的试剂。

8. 发明申请

WO2005027725A3 DETERMINATION OF IONS USING ION-SENSITIVE ENZYMES 审中-公开
标题翻译：使用敏感酶测定离子
公开(公告)号：WO2005027725A3
公开(公告)日：2006-08-24
申请号：PCT/US2004030522
申请日：2004-09-17
申请人： GEN ATOMICS , YUAN CHONG-SHENG
发明人： YUAN CHONG-SHENG
IPC分类号： C12N9/16 , A61B20060101 , C07K14/705 , C12N9/22 , C12N15/55 , C12P21/02 , G01N33/53 , G01N33/84
CPC分类号： G01N33/84
摘要： The invention relates generally to the field of sodium and lithium ion detection. In particular, the invention provides chimeric proteins, nucleic acids encoding chimeric proteins, methods and kits for assaying for sodium ions and for lithium ions in a sample, using inter alia, a 3'(2'),5'-bisphosphate nucleotidase.
摘要翻译：本发明一般涉及钠离子和锂离子检测领域。特别地，本发明提供嵌合蛋白质，编码嵌合蛋白质的核酸，用于测定样品中的钠离子和锂离子的方法和试剂盒，特别是使用3'（2'），5'-二磷酸核苷酸酶。

9. 发明申请

WO2006065967A2 ALLOSTERIC ENZYME COUPLED IMMUNOASSAY (AECIA) 审中-公开
标题翻译： ALLOSTERIC ENZYME偶联免疫（AECIA）
公开(公告)号：WO2006065967A2
公开(公告)日：2006-06-22
申请号：PCT/US2005/045374
申请日：2005-12-15
申请人： GENERAL ATOMICS , YUAN, Chong-Sheng
发明人： YUAN, Chong-Sheng
CPC分类号： G01N33/78 , C12Q1/485 , G01N33/542 , G01N33/54393 , G01N33/581 , G01N2333/91215
摘要： The present invention is directed to methods and compositions for an allosteric enzyme coupled assay, and preferably to an allosteric enzyme coupled immunoassay (AECIA). The assay uses an allosteric enzyme to generate a readout signal. The assay is based on the competition between an analyte in a sample and an analyte or analyte analog conjugated to an allosteric regulator with a specific binding reagent for the analyte. In the absence of any analyte in the sample, an analyte or an analyte analog conjugated to an allosteric regulator binds to the specific binding reagent and such binding prevents or reduces the allosteric regulator's regulation, e.g., activation, on the allosteric enzyme. An analyte, if present in the sample, competes with the analyte or analyte analog conjugated to the allosteric regulator for binding with the specific binding reagent, reduces or prevents binding of the specific binding reagent to the analyte or analyte analog conjugated to the allosteric regulator, leading to increased regulation, e.g., activation, of the enzyme. 1-substituted-(3-D-fructofuranose 2, 6bisphosphate compounds and conjugates comprising the same are provided. Kits comprising the conjugates, and methods using the conjugates for assaying an analyte are further provided.
摘要翻译：本发明涉及用于变构酶偶联测定法，优选变构酶偶联免疫测定法（AECIA）的方法和组合物。该测定使用变构酶来产生读出信号。该测定法基于样品中的分析物与与变构调节剂缀合的分析物或分析物类似物与分析物的特异性结合试剂之间的竞争。在样品中没有任何分析物的情况下，与变构调节物缀合的分析物或分析物类似物与特异性结合试剂结合，并且这种结合在变构酶上阻止或减少变构调节物的调节，例如活化。分析物（如果存在于样品中）与与变构调节剂缀合的分析物或分析物类似物与特异性结合试剂结合竞争，减少或防止特异性结合试剂与分子或与变构调节物结合的分析物类似物的结合，导致酶的调节，例如活化。还提供了1-取代的（3-D-呋喃果糖）2,6β-磷酸酯化合物和包含它们的缀合物。包含缀合物的试剂盒和使用共轭物用于分析分析物的方法进一步提供。

10. 发明申请

WO2006044573A3 REVERSIBLE INHIBITORS OF S-ADENOSYL-L-HOMOCYSTEINE HYDROLASE AND USES THEREOF 审中-公开
公开(公告)号：WO2006044573A3
公开(公告)日：2006-04-27
申请号：PCT/US2005/036890
申请日：2005-10-13
申请人： GENERAL ATOMICS , YUAN, Chong-Sheng
发明人： YUAN, Chong-Sheng
IPC分类号： C07D471/04 , C07D473/18 , C07D473/34 , C07D473/16 , C07D473/40 , A61K31/52 , A61K31/522 , A61K31/437 , A61P35/00 , A61P31/20 , A61P37/06 , A61P25/28 , A61P3/10
摘要： The present invention provides compositions and methods for reversibly inhibiting Sadenosyl-L-homocysteine (SAH) hydrolase. The compounds of the present invention can be used in combination with an anti-hemorrhagic viral infection agent, an immunosuppressant, a homocysteine lowering agent, or an anti-neoplasm agent. The compositions and methods of the present invention can be used for the prevention and treatment of hemorrhagic virus infection, autoimmune diseases, autograft rejection, neoplasm, hyperhomocysteineuria, cardiovascular disease, stroke, Alzheimer's disease, or diabetes.

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