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1. 发明申请

WO2006029538A1 SIALYLTRANSFERASES COMRISING CONSERVED SEQUENCE MOTIFS 审中-公开
标题翻译：综合考察保守序列运动
公开(公告)号：WO2006029538A1
公开(公告)日：2006-03-23
申请号：PCT/CA2005/001432
申请日：2005-09-16
申请人： NATIONAL RESEARCH COUNCIL OF CANADA , GILBERT, Michel , WAKARCHUK, Warren, W.
发明人： GILBERT, Michel , WAKARCHUK, Warren, W.
IPC分类号： C12N15/54 , C12N9/10 , C12P21/02 , C12P19/00
CPC分类号： C12P19/26 , C12N9/1081
摘要： The present invention provides, e.g ., sialyltransferase proteins comprising conserved sequence motifs, including α-2,3-sialyltransferase proteins from C. jejuni strains O:36 and O:19. The invention also provides methods of making sialylated products using those sialyltransferases.
摘要翻译：本发明提供了例如包含保守序列基序的唾液酸转移酶蛋白，包括空肠弯曲杆菌菌株O：36和O：19的α-2,3唾液酸转移酶蛋白。本发明还提供了使用这些唾液酸转移酶制备唾液酸化产物的方法。

2. 发明申请

WO2002074942A2 CAMPYLOBACTER GLYCOSYLTRANSFERASES FOR BIOSYNTHESIS OF GANGLIOSIDES AND GANGLIOSIDE MIMICS 审中-公开
标题翻译：姜黄素和甘油三酯的生物合成的果胶糖苷
公开(公告)号：WO2002074942A2
公开(公告)日：2002-09-26
申请号：PCT/CA2002/000229
申请日：2002-02-22
申请人： NATIONAL RESEARCH COUNCIL OF CANADA , GILBERT, Michel , WAKARCHUK, Warren, W.
发明人： GILBERT, Michel , WAKARCHUK, Warren, W.
IPC分类号： C12N9/00
CPC分类号： C12N9/1241 , C12N9/1029 , C12N9/1048 , C12N9/1051 , C12N9/1081 , C12P19/18
摘要： This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an α2,3- and an α2,8-activity. A β1,4-GalNAc transferase and a β1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni . In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.
摘要翻译：本发明提供了原核糖基转移酶，包括具有α2B和α2,8-活性的双官能唾液酸转移酶。本发明还提供了β1,4-GalNAc转移酶和β1,3-半乳糖基转移酶，以及参与脂寡糖（LOS）合成的其它糖基转移酶和酶也是如此。糖基转移酶可以从例如弯曲杆菌属物种获得，包括空肠弯曲杆菌。在另外的实施方案中，本发明提供编码糖基转移酶的核酸，以及用于表达糖基转移酶的表达载体和宿主细胞。

3. 发明申请

WO1994024270A3 STABLE MUTANTS OF A LOW MOLECULAR MASS XYLANASE 审中-公开
公开(公告)号：WO1994024270A3
公开(公告)日：1994-10-27
申请号：PCT/CA1994000180
申请日：1994-04-07
申请人： NATIONAL RESEARCH COUNCIL OF CANADA , CAMPBELL, Robert, L. , ROSE, David, R. , SUNG, Wing, L. , YAGUCHI, Makoto , WAKARCHUK, Warren, W. , ISHIKAWA, Kazuhiko
发明人： NATIONAL RESEARCH COUNCIL OF CANADA
IPC分类号： C12N09/24

4. 发明申请

WO9424270A2 STABLE MUTANTS OF A LOW MOLECULAR MASS XYLANASE 审中-公开
标题翻译：低分子量XYLANASE的稳定突变体
公开(公告)号：WO9424270A2
公开(公告)日：1994-10-27
申请号：PCT/CA9400180
申请日：1994-04-07
申请人： CA NAT RESEARCH COUNCIL , CAMPBELL ROBERT L , ROSE DAVID R , SUNG WING L , YAGUCHI MAKOTO , WAKARCHUK WARREN W , ISHIKAWA KAZUHIKO
发明人： CAMPBELL ROBERT L , ROSE DAVID R , SUNG WING L , YAGUCHI MAKOTO , WAKARCHUK WARREN W , ISHIKAWA KAZUHIKO
IPC分类号： C12N9/24 , D21C5/00
CPC分类号： C12Y302/01032 , C12N9/248 , C12Y302/01008 , D21C5/005
摘要： The stability of the 20,396 dalton Bacillus circulans xylanase was increased by site-directed mutagenesis. Increased stability was conferred by the presence of non-native disulfide bridges, and selected N-terminal mutations. The introduction of these non-native disulfide bridges was accomplished by the examination of the three-dimensional structure of the enzyme, and choosing sites where a favorable geometry for a bridge existed. The N-terminal mutations were constructed on the basis of primary sequence comparison with other family G xylanases. The mutant proteins were examined: for their ability to retain enzymatic activity after heating, as an indication of increased thermostability; for their ability to function at elevated temperatures and for their ability to function at a more basic pH. These more stable variants are useful as an alternative to chemical bleaching of Kraft pulp in a pre-bleaching step (bio-bleaching). The pre-bleaching step involves higher temperature and pH than that normally used for these enzymes, and accordingly these variants can be advantageously used at this step. These stable xylanases are also of use in the food processing industry.
摘要翻译：通过定点诱变增加了20396个达尔顿环状芽孢杆菌木聚糖酶的稳定性。非天然二硫键的存在和选择的N端突变赋予了稳定性的提高。这些非天然二硫键的引入是通过检查酶的三维结构，并选择存在桥的有利几何形状的位点来实现的。基于与其他家族G木聚糖酶的初级序列比较构建N末端突变。检测突变蛋白：加热后保留酶活性的能力，作为增加热稳定性的指标; 因为它们在升高的温度下起作用和在更碱性的pH下起作用的能力。这些更稳定的变体可用作在漂白前步骤（生物漂白）中牛皮纸浆的化学漂白的替代物。预漂白步骤涉及比通常用于这些酶的更高的温度和pH，因此这些变体可以有利地用于该步骤。这些稳定的木聚糖酶也可用于食品加工业。

5. 发明申请

WO02074942B1 CAMPYLOBACTER GLYCOSYLTRANSFERASES FOR BIOSYNTHESIS OF GANGLIOSIDES AND GANGLIOSIDE MIMICS 审中-公开
标题翻译：姜黄素和甘油三酯的生物合成的果胶糖苷
公开(公告)号：WO02074942B1
公开(公告)日：2003-07-03
申请号：PCT/CA0200229
申请日：2002-02-22
申请人： CA NAT RESEARCH COUNCIL , GILBERT MICHEL , WAKARCHUK WARREN W
发明人： GILBERT MICHEL , WAKARCHUK WARREN W
IPC分类号： C12N15/09 , C12N1/21 , C12N9/10 , C12N9/12 , C12N9/88 , C12N15/54 , C12P19/18 , C12N5/10 , C12Q1/68
CPC分类号： C12N9/1241 , C12N9/1029 , C12N9/1048 , C12N9/1051 , C12N9/1081 , C12P19/18
摘要： This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an alpha 2,3- and an alpha 2,8-activity. A beta 1,4-GalNAc transferase and a beta 1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.
摘要翻译：本发明提供了原核糖基转移酶，包括具有α2B和α2,8-活性的双官能唾液酸转移酶。本发明还提供了β1,4-GalNAc转移酶和β1,3-半乳糖基转移酶，以及参与脂寡糖（LOS）合成的其它糖基转移酶和酶也是如此。糖基转移酶可以从例如弯曲杆菌属物种获得，包括空肠弯曲杆菌。在另外的实施方案中，本发明提供编码糖基转移酶的核酸，以及用于表达糖基转移酶的表达载体和宿主细胞。

6. 发明申请

WO2006029538A8 SIALYLTRANSFERASES COMPRISING CONSERVED SEQUENCE MOTIFS 审中-公开
标题翻译：包含保守序列运动的SIALYLDRANSFERASES
公开(公告)号：WO2006029538A8
公开(公告)日：2006-06-01
申请号：PCT/CA2005001432
申请日：2005-09-16
申请人： CA NAT RESEARCH COUNCIL , GILBERT MICHEL , WAKARCHUK WARREN W
发明人： GILBERT MICHEL , WAKARCHUK WARREN W
IPC分类号： C12N15/54 , C12N9/10 , C12P19/00 , C12P21/02
CPC分类号： C12P19/26 , C12N9/1081
摘要： The present invention provides, e.g., sialyltransferase proteins comprising conserved sequence motifs, including a-2,3-sialyltransferase proteins from C. jejuni strains O:36 and O:19. The invention also provides methods of making sialylated products using those sialyltransferases.
摘要翻译：本发明提供了例如包含保守序列基序的唾液酸转移酶蛋白，包括空肠弯曲杆菌菌株O：36和O：19的2,3-唾液酸转移酶蛋白。本发明还提供使用这些唾液酸转移酶制备唾液酸化产物的方法。

7. 发明申请

WO0248320A3 CRYSTAL STRUCTURES OF RETAINING GLYCOSYLTRANSFERASES 审中-公开
标题翻译：保留谷氨酰转移酶的晶体结构
公开(公告)号：WO0248320A3
公开(公告)日：2002-12-12
申请号：PCT/CA0101793
申请日：2001-12-14
申请人： UNIV BRITISH COLUMBIA , WITHERS STEPHEN G , WAKARCHUK WARREN W , STRYNADKA NATALIE C J , DIECKELMANN MANUELA , LY HOA , PERSSON KARINA
发明人： WITHERS STEPHEN G , WAKARCHUK WARREN W , STRYNADKA NATALIE C J , DIECKELMANN MANUELA , LY HOA , PERSSON KARINA
IPC分类号： C12N9/10
CPC分类号： C12N9/104 , C07K2299/00 , C12N9/1051
摘要： The present invention relates to a crystal comprising the ligand binding pocket of a glycosyltransferase enzyme and optionally a donor molecule or analogue thereof and/or an acceptor molecule or analogue thereof. The present invention also relates to the use of such a crystal to identify ligands capable of modulating glycosyltransferase activity, and the use of such ligands in therapeutic applications.
摘要翻译：本发明涉及包含糖基转移酶的配体结合口袋和任选的供体分子或其类似物和/或受体分子或其类似物的晶体。本发明还涉及这种晶体鉴定能够调节糖基转移酶活性的配体的用途，以及这些配体在治疗应用中的用途。

8. 发明申请

WO2002074942A3 CAMPYLOBACTER GLYCOSYLTRANSFERASES FOR BIOSYNTHESIS OF GANGLIOSIDES AND GANGLIOSIDE MIMICS 审中-公开
公开(公告)号：WO2002074942A3
公开(公告)日：2002-09-26
申请号：PCT/CA2002/000229
申请日：2002-02-22
申请人： NATIONAL RESEARCH COUNCIL OF CANADA , GILBERT, Michel , WAKARCHUK, Warren, W.
发明人： GILBERT, Michel , WAKARCHUK, Warren, W.
IPC分类号： C12N9/10
摘要： This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an α2,3- and an α2,8-activity. A β1,4-GalNAc transferase and a β1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni . In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.

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