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1. 发明专利

CA2126299A1 Parathyroid Hormone Analogues for the Treatment of Osteoporosis 未知
公开(公告)号：CA2126299A1
公开(公告)日：1995-12-21
申请号：CA2126299
申请日：1994-06-20
申请人： WILLICK GORDON E , WHITFIELD JAMES F , SUREWICZ WITOLD , SUNG WING L , NEUGEBAUER WITOLD
发明人： WILLICK GORDON E , WHITFIELD JAMES F , SUREWICZ WITOLD , SUNG WING L , NEUGEBAUER WITOLD
IPC分类号： A61K38/00 , C07K14/635 , C07K7/54
摘要： Certain analogues of human parathyroid hormone (hPTH) have been found to be effective for the treatment of osteoporosis, while showing decreased side effects. Analogues showing this effect include all sequences from hPTH-(1-28)-NH2 to hPTH-(1-31)-NH2 and all sequences from [Leu27]-hPTH-(1-28)-NH2 to [Leu27]-hPTH-(1-33)-NH2. Also included are cyclic analogues cyclo(Lys26-Asp30) [Leu27]-hPTH-(1-34)NH2 and cyclo (Lys27-Asp30 )-hPTH-(1-34)-NH2. Analogues in the form of the carboxyl terminal amide are particularly effective.

2. 发明专利

CA2126299C PARATHYROID HORMONE ANALOGUES FOR THE TREATMENT OF OSTEOPOROSIS 未知
公开(公告)号：CA2126299C
公开(公告)日：2000-12-12
申请号：CA2126299
申请日：1994-06-20
申请人： WILLICK GORDON E , WHITFIELD JAMES F , NEUGEBAUER WITOLD , SUREWICZ WITOLD , RIXON RAYMOND H , SUNG WING L
发明人： WILLICK GORDON E , WHITFIELD JAMES F , NEUGEBAUER WITOLD , SUREWICZ WITOLD , RIXON RAYMOND H , SUNG WING L
IPC分类号： A61K38/00 , C07K14/635 , C07K7/54
摘要： Certain analogues of human parathyroid hormone (hPTH) have been found to be effective for the treatment of osteoporosis, while showing decreased side effects. Analogues showing this effect include all sequences from hPTH-(1-28)-NH2 to hPTH (1-31)-NH2 and all sequences from ¢Leu27!-hPTH-(1-28)-NH2 to ¢Leu27!-hPTH-(1-34)-NH2. Also included are cyclic analogues cyclo(Lys26-Asp30)¢Leu27!-hPTH-(1-34)NH2 and cyclo (Lys27-Asp30)-hPTH-(1-34)-NH2. Analogues in the form of the carboxyl terminal amide are particularly effective.

3. 发明申请

WO2010081213A1 ENZYMATIC PREPARATION OF PLANT FIBERS 审中-公开
标题翻译：植物纤维的酶化制备
公开(公告)号：WO2010081213A1
公开(公告)日：2010-07-22
申请号：PCT/CA2009/001886
申请日：2009-12-23
申请人： NATIONAL RESEARCH COUNCIL OF CANADA , SUNG, Wing L. , WOOD, Mark , HUANG, Fang
发明人： SUNG, Wing L. , WOOD, Mark , HUANG, Fang
IPC分类号： D01C1/02
CPC分类号： D01C1/02
摘要： A method of extracting fibers from decorticated plant bast skin involves pre-treating decorticated plant bast skin of a fiber plant with an aqueous solution containing trisodium citrate having a pH in a range of about 8-14 at a temperature of about 9O°C or less; and subsequently treating recovered fibers with a protease at alkaline pH.
摘要翻译：从脱胶的植物皮肤皮肤提取纤维的方法包括用含约柠檬酸三钠的水溶液预处理纤维植物的脱皮植物皮肤，所述柠檬酸三钠在约90℃或更低的温度下pH约为8-14 ; 并随后在碱性pH下用蛋白酶处理回收的纤维。

4. 发明申请

WO2007115407A1 MODIFICATION OF XYLANASES TO INCREASE THERMOPHILICITY, THERMOSTABILITY AND ALKALOPHILICITY 审中-公开
标题翻译：修改木聚糖酶以增加热亲和性，热稳定性和碱性亲和性
公开(公告)号：WO2007115407A1
公开(公告)日：2007-10-18
申请号：PCT/CA2007/000590
申请日：2007-04-10
申请人： NATIONAL RESEARCH COUNCIL OF CANADA , SUNG, Wing, L.
发明人： SUNG, Wing, L.
IPC分类号： C12N9/24 , C12N15/31 , C12N15/56 , C12Q1/34
CPC分类号： C12N9/2482 , C12Y302/01008 , D21C5/005 , D21C9/1036 , D21H17/005
摘要： A modified Family 11 xylanase enzyme comprising cysteine residues at positions 99 and 118 to form an intramolecular disulfide bond is provided. The modified xylanase is produced by substitution of an amino acid at position 99, 118 or both positions 99 and 118 with a cysteine to produce the intramolecular disulfide bond. Xylanases of the invention display improved thermophilicity, alkalophilicity or thermostability relative to wild-type xylanases. Such xylanases find use in a variety of applications in industry that require enzyme activities at temperatures and/or pH values above that of the native enzyme.
摘要翻译：提供了包含位置99和118上的半胱氨酸残基以形成分子内二硫键的经修饰的家族11木聚糖酶。通过用半胱氨酸取代位置99,118或位置99和118的氨基酸以产生分子内二硫键来生产修饰的木聚糖酶。相对于野生型木聚糖酶，本发明的木聚糖酶显示出改善的嗜热性，嗜碱性或热稳定性。这种木聚糖酶可用于工业中需要在天然酶温度和/或pH值以上的温度下进行酶活性的各种应用。

5. 发明申请

WO0192487A3 MODIFIED XYLANASES EXHIBITING INCREASED THERMOPHILICITY AND ALKALOPHILICITY 审中-公开
标题翻译：改良的XYLANASES展现出增加的热力和碱性
公开(公告)号：WO0192487A3
公开(公告)日：2002-09-26
申请号：PCT/CA0100769
申请日：2001-05-31
申请人： CA NAT RESEARCH COUNCIL , SUNG WING L
发明人： SUNG WING L
IPC分类号： C12N20060101 , C12N9/24 , C12N15/56
CPC分类号： C12N9/2482 , C12Y302/01008
摘要： The present invention pertains to modified xylanase enzymes that exhibit increased thermostability and alkalophilicity, when compared with their native counterparts. Several modified xylanases exhibiting these properties are disclosed including xylanases with at least one modification at amino acid position (10, 27, 29, 75, 104, 105, 125, 129, 132, 135, 144, 157, 161, 162, 165) or a combination thereof. Also included within the present invention is a modified xylanase that comprise at least one substituted amino acid residue and that may be characterized as having a maximum effective temperature (MET) between about 69 DEG C to about 78 DEG C, wherein the modified xylanase is a Family 11 xylanase obtained from a Trichoderma sp.. The present invention also includes a modified Family 11 xylanase obtained from a Trichoderma sp. characterized as having a maximum effective pH (MEP) between about 5.8 to about 7.6. Modified xylanases characterized as having a MET between about 69 DEG C to about 78 DEG C and a MEP between about 5.8 to about 7.6 are also disclosed.
摘要翻译：本发明涉及与其天然对应物相比显示增加的热稳定性和嗜碱性的改性木聚糖酶。公开了显示这些性质的几种改性的木聚糖酶，其包括在氨基酸位置（10,27,29，75,104,105,125,129,132,135,144,157,161,162,165）具有至少一个修饰的木聚糖酶。或其组合。本发明还包括修饰的木聚糖酶，其包含至少一个取代的氨基酸残基并且可以被表征为具有在约69℃至约78℃之间的最大有效温度（MET），其中所述改性的木聚糖酶是从木霉属菌种获得的家族11木聚糖酶。本发明还包括从木霉属（Strichoderma）sp。获得的经修饰的家族11木聚糖酶。其特征在于具有在约5.8至约7.6之间的最大有效pH（MEP）。还公开了特征在于具有在约69℃至约78℃之间的MET和约5.8至约7.6之间的MEP的改性木聚糖酶。

6. 发明申请

WO1991005050A1 SYNTHESIS OF MATURE HUMAN PARATHYROID HORMONE 审中-公开
标题翻译：成人人体PARAMYROID HORMONE的合成
公开(公告)号：WO1991005050A1
公开(公告)日：1991-04-18
申请号：PCT/CA1990000335
申请日：1990-10-01
申请人： (HER) MAJESTY IN RIGHT OF CANADA, as represented ... , SUNG, Wing, L.
发明人： (HER) MAJESTY IN RIGHT OF CANADA, as represented ...
IPC分类号： C12N15/16
CPC分类号： C07K14/635 , C12N15/67
摘要： A nucleotide coding sequence for human parathyroid hormone has been synthesized, which sequence results in a substantially higher yield of mature PTH than hitherto could be obtained. This is achieved by providing a synthesized nucleotide sequence coding for mature human PTH or a biologically active analog, wherein the amino terminal coding sequence is adenine-rich. A sequence wherein the degenerate codons for some or all of amino acids 1 through 5 are adenine-rich is preferred.
摘要翻译：已经合成了人甲状旁腺激素的核苷酸编码序列，该序列导致比迄今为止可获得的成熟PTH的产量显着更高。这通过提供编码成熟人PTH或生物活性类似物的合成核苷酸序列来实现，其中氨基末端编码序列是富含腺嘌呤的。一些序列，其中一些或全部氨基酸1至5的简并密码子是富含腺嘌呤的序列。

7. 发明申请

WO1994024270A3 STABLE MUTANTS OF A LOW MOLECULAR MASS XYLANASE 审中-公开
公开(公告)号：WO1994024270A3
公开(公告)日：1994-10-27
申请号：PCT/CA1994000180
申请日：1994-04-07
申请人： NATIONAL RESEARCH COUNCIL OF CANADA , CAMPBELL, Robert, L. , ROSE, David, R. , SUNG, Wing, L. , YAGUCHI, Makoto , WAKARCHUK, Warren, W. , ISHIKAWA, Kazuhiko
发明人： NATIONAL RESEARCH COUNCIL OF CANADA
IPC分类号： C12N09/24

8. 发明申请

WO9424270A2 STABLE MUTANTS OF A LOW MOLECULAR MASS XYLANASE 审中-公开
标题翻译：低分子量XYLANASE的稳定突变体
公开(公告)号：WO9424270A2
公开(公告)日：1994-10-27
申请号：PCT/CA9400180
申请日：1994-04-07
申请人： CA NAT RESEARCH COUNCIL , CAMPBELL ROBERT L , ROSE DAVID R , SUNG WING L , YAGUCHI MAKOTO , WAKARCHUK WARREN W , ISHIKAWA KAZUHIKO
发明人： CAMPBELL ROBERT L , ROSE DAVID R , SUNG WING L , YAGUCHI MAKOTO , WAKARCHUK WARREN W , ISHIKAWA KAZUHIKO
IPC分类号： C12N9/24 , D21C5/00
CPC分类号： C12Y302/01032 , C12N9/248 , C12Y302/01008 , D21C5/005
摘要： The stability of the 20,396 dalton Bacillus circulans xylanase was increased by site-directed mutagenesis. Increased stability was conferred by the presence of non-native disulfide bridges, and selected N-terminal mutations. The introduction of these non-native disulfide bridges was accomplished by the examination of the three-dimensional structure of the enzyme, and choosing sites where a favorable geometry for a bridge existed. The N-terminal mutations were constructed on the basis of primary sequence comparison with other family G xylanases. The mutant proteins were examined: for their ability to retain enzymatic activity after heating, as an indication of increased thermostability; for their ability to function at elevated temperatures and for their ability to function at a more basic pH. These more stable variants are useful as an alternative to chemical bleaching of Kraft pulp in a pre-bleaching step (bio-bleaching). The pre-bleaching step involves higher temperature and pH than that normally used for these enzymes, and accordingly these variants can be advantageously used at this step. These stable xylanases are also of use in the food processing industry.
摘要翻译：通过定点诱变增加了20396个达尔顿环状芽孢杆菌木聚糖酶的稳定性。非天然二硫键的存在和选择的N端突变赋予了稳定性的提高。这些非天然二硫键的引入是通过检查酶的三维结构，并选择存在桥的有利几何形状的位点来实现的。基于与其他家族G木聚糖酶的初级序列比较构建N末端突变。检测突变蛋白：加热后保留酶活性的能力，作为增加热稳定性的指标; 因为它们在升高的温度下起作用和在更碱性的pH下起作用的能力。这些更稳定的变体可用作在漂白前步骤（生物漂白）中牛皮纸浆的化学漂白的替代物。预漂白步骤涉及比通常用于这些酶的更高的温度和pH，因此这些变体可以有利地用于该步骤。这些稳定的木聚糖酶也可用于食品加工业。

9. 发明申请

WO2007115391A1 MODIFICATION OF XYLANASES TO INCREASE THERMOPHILICITY, THERMOSTABILITY AND ALKALOPHILICITY 审中-公开
标题翻译：改善XYLANASES以提高热稳定性，热稳定性和碱性
公开(公告)号：WO2007115391A1
公开(公告)日：2007-10-18
申请号：PCT/CA2006/001192
申请日：2006-07-19
申请人： NATIONAL RESEARCH COUNCIL OF CANANDA , SUNG, Wing, L.
发明人： SUNG, Wing, L.
IPC分类号： C12N9/24 , D21C9/10 , D21H17/22 , D21H25/02 , C12H1/15 , C12G1/022 , C12N15/56
CPC分类号： C12N9/2482 , C12Y302/01008 , D21C5/005 , D21C9/1036 , D21H17/005
摘要： A modified Family (11) xylanase enzyme comprising cysteine residues at positions (99) and (118) to form an intramolecular disulfide bond is provided. The modified xylanase is produced by substitution of an amino acid at position (99, 118) or both positions (99) and (118) with a cysteine to produce the intramolecular disulfide bond. Xylanases of the invention display improved thermophilicity, alkalophilicity or thermostability relative to wild-type xylanases. Such xylanases find use in a variety of applications in industry that require enzyme activities at temperatures and/or pH values above that of the native enzyme.
摘要翻译：提供了在位置（99）和（118）处包含半胱氨酸残基以形成分子内二硫键的经修饰的家族（11）木聚糖酶。通过用位置（99,118）或两个位置（99）和（118）的氨基酸与半胱氨酸取代来产生修饰的木聚糖酶以产生分子内二硫键。本发明的木聚糖酶相对于野生型木聚糖酶显示出改善的嗜热性，嗜碱性或热稳定性。这种木聚糖酶可用于工业中需要酶活性的各种应用，温度和/或pH值高于天然酶的温度和/或pH值。

10. 发明申请

WO2007115407B1 MODIFICATION OF XYLANASES TO INCREASE THERMOPHILICITY, THERMOSTABILITY AND ALKALOPHILICITY 审中-公开
标题翻译：改善XYLANASES以提高热稳定性，热稳定性和碱性
公开(公告)号：WO2007115407B1
公开(公告)日：2007-12-21
申请号：PCT/CA2007000590
申请日：2007-04-10
申请人： CA NAT RESEARCH COUNCIL , SUNG WING L
发明人： SUNG WING L
IPC分类号： C12N9/24 , C12N15/31 , C12N15/56 , C12Q1/34
CPC分类号： C12N9/2482 , C12Y302/01008 , D21C5/005 , D21C9/1036 , D21H17/005
摘要： A modified Family 11 xylanase enzyme comprising cysteine residues at positions 99 and 118 to form an intramolecular disulfide bond is provided. The modified xylanase is produced by substitution of an amino acid at position 99, 118 or both positions 99 and 118 with a cysteine to produce the intramolecular disulfide bond. Xylanases of the invention display improved thermophilicity, alkalophilicity or thermostability relative to wild-type xylanases. Such xylanases find use in a variety of applications in industry that require enzyme activities at temperatures and/or pH values above that of the native enzyme.
摘要翻译：提供了修饰的家族11木聚糖酶，其包含位置99和118处的半胱氨酸残基以形成分子内二硫键。经修饰的木聚糖酶是通过用半胱氨酸取代位置99,118或位置99和118的氨基酸产生分子内二硫键而产生的。本发明的木聚糖酶相对于野生型木聚糖酶显示出改善的嗜热性，嗜碱性或热稳定性。这种木聚糖酶可用于工业中需要酶活性的各种应用，温度和/或pH值高于天然酶的温度和/或pH值。

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