专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08980579B2 Chromosomal landing pads and related uses 有权
标题翻译：染色体着陆垫及相关用途
公开(公告)号：US08980579B2
公开(公告)日：2015-03-17
申请号：US13440661
申请日：2012-04-05
申请人： Vincent P. Mauro , Wei Zhou , Bruce Cunningham , Gerald M. Edelman
发明人： Vincent P. Mauro , Wei Zhou , Bruce Cunningham , Gerald M. Edelman
IPC分类号： C12P21/06 , C12N15/90 , C12P21/04 , C12N5/07 , C12N5/10 , C12N15/00 , C12N15/87
CPC分类号： C12N15/907 , C12N2320/32 , C12N2800/107 , C12N2800/30
摘要： Provided herein are methods for stable integration and/or expression of one or more recombinant polynucleotides in a host cell. The recombinant polynucleotides are typically integrated into the host genome at some native chromosomal integration sites. The integration can be mediated by homologous recombination or by using a hybrid recombinase targeting the specific chromosomal locations. The native chromosomal integration sites in the host cells, which support stable integration and strong transcription activities of foreign genes, are present within or adjacent to specific genes in the CHO genome, the ankyrin 2 gene (Ank2), cleavage and polydenylation specific factor 4 gene (Cpsf4), C-Mos gene, and Nephrocystin-1/Mal gene. Also provided are methods and nucleic acid molecules for inserting site-specific recombination sequences (chromosomal landing pads) into these specific chromosomal locations, engineered host cells containing chromosomal landing pads, methods and compositions (e.g., kits) therefore.
摘要翻译：本文提供了用于在宿主细胞中稳定整合和/或表达一种或多种重组多核苷酸的方法。重组多核苷酸通常在某些天然染色体整合位点整合到宿主基因组中。整合可以通过同源重组或通过使用靶向特定染色体位置的杂交重组酶来介导。宿主细胞中的天然染色体整合位点，其支持外源基因的稳定整合和强的转录活性，存在于CHO基因组，锚蛋白2基因（Ank2），切割和聚乙二醇化特异性因子4基因中的特定基因内或附近（Cpsf4），C-Mos基因和Nephrocystin-1 / Mal基因。还提供了用于将位点特异性重组序列（染色体着陆垫）插入这些特定染色体位置的方法和核酸分子，因此含有染色体着陆垫的工程化宿主细胞，方法和组合物（例如，试剂盒）。

2. 发明申请

US20090093049A1 Methods of Identifying Synthetic Transcriptional and Translational Regulatory Elements, and Compositions Related to Same 审中-公开
标题翻译：识别合成转录调节元件及其相关组成的方法
公开(公告)号：US20090093049A1
公开(公告)日：2009-04-09
申请号：US12266103
申请日：2008-11-06
申请人： Vincent P. Mauro , Gerald M. Edelman , Stephen A. Chappell , Frederick S. Jones , Geoffrey Owens , Robyn Meech
发明人： Vincent P. Mauro , Gerald M. Edelman , Stephen A. Chappell , Frederick S. Jones , Geoffrey Owens , Robyn Meech
IPC分类号： C12N15/63 , C07H21/02
CPC分类号： C12N15/90 , C12N15/1048 , C12N15/1051 , C12N15/67 , C12N15/86 , C12N2740/13043 , C12N2800/108 , C12N2830/002 , C12N2830/15 , C12N2840/203
摘要： Provided are methods of identifying oligonucleotides having transcriptional or translational activity by integrating ilie oligonucleotide into a eukaryotic cell genome such that the oligonucleotide is operatively linked to an expressible polynucleotide, and detecting a change in expression of the expressible polynucleotide due to the operatively linked oligonucleotide. Also provided are vectors useful for identifying an oligonucleotide having transcriptional or translational regulatory activity according to a method of the invention. In addition, isolated synthetic transcriptional or translational regulatory elements identified according to a method of the invention are provided, as are kits, which contain a vector useful for identifying a transcriptional or translational regulatory element, or an isolated synthetic transcriptional or translational regulatory element or plurality of such elements. Also provided are isolated transcriptional regulatory elements.
摘要翻译：提供了通过将ilie寡核苷酸整合到真核细胞基因组中来鉴定具有转录或翻译活性的寡核苷酸的方法，使得寡核苷酸可操作地连接到可表达的多核苷酸，并且由于可操作地连接的寡核苷酸检测可表达的多核苷酸的表达变化。还提供了根据本发明的方法用于鉴定具有转录或翻译调节活性的寡核苷酸的载体。此外，提供了根据本发明的方法鉴定的分离的合成转录或翻译调节元件，试剂盒还包含含有用于鉴定转录或翻译调控元件的载体或分离的合成转录或翻译调控元件或多个的这些元素。还提供了分离的转录调控元件。

3. 发明授权

US07456273B2 Methods of identifying synthetic transcriptional and translational regulatory elements, and compositions relating to same 失效
标题翻译：识别合成转录和翻译调节元件的方法以及与其相关的组合物
公开(公告)号：US07456273B2
公开(公告)日：2008-11-25
申请号：US11607131
申请日：2006-11-29
申请人： Vincent P. Mauro , Gerald M. Edelman , Stephen A. Chappell , Frederick S. Jones , Geoffrey Owens , Robyn Meech
发明人： Vincent P. Mauro , Gerald M. Edelman , Stephen A. Chappell , Frederick S. Jones , Geoffrey Owens , Robyn Meech
IPC分类号： C07H21/04 , C07H21/02 , C12Q1/68
CPC分类号： C12N15/90 , C12N15/1048 , C12N15/1051 , C12N15/67 , C12N15/86 , C12N2740/13043 , C12N2800/108 , C12N2830/002 , C12N2830/15 , C12N2840/203
摘要： Provided are methods of identifying oligonucleotides having transcriptional or translational activity by integrating ilie oligonucleotide into a eukaryotic cell genome such that the oligonucleotide is operatively linked to an expressible polynucleotide, and detecting a change in expression of the expressible polynucleotide due to the operatively linked oligonucleotide. Also provided are vectors useful for identifying an oligonucleotide having transcriptional or translational regulatory activity according to a method of the invention. In addition, isolated synthetic transcriptional or translational regulatory elements identified according to a method of the invention are provided, as are kits, which contain a vector useful for identifying a transcriptional or translational regulatory element, or an isolated synthetic transcriptional or translational regulatory element or plurality of such elements. Also provided are isolated transcriptional regulatory elements.
摘要翻译：提供了通过将ilie寡核苷酸整合到真核细胞基因组中来鉴定具有转录或翻译活性的寡核苷酸的方法，使得寡核苷酸可操作地连接到可表达的多核苷酸，并且由于可操作地连接的寡核苷酸检测可表达的多核苷酸的表达变化。还提供了根据本发明的方法用于鉴定具有转录或翻译调节活性的寡核苷酸的载体。此外，提供了根据本发明的方法鉴定的分离的合成转录或翻译调节元件，试剂盒还包含含有用于鉴定转录或翻译调控元件的载体或分离的合成转录或翻译调控元件或多个的这些元素。还提供了分离的转录调控元件。

4. 发明授权

US08350020B2 Compositions and methods related to mRNA translational enhancer elements 有权
公开(公告)号：US08350020B2
公开(公告)日：2013-01-08
申请号：US12316581
申请日：2008-12-11
申请人： Vincent P. Mauro , Gerald M. Edelman , Wei Zhou
发明人： Vincent P. Mauro , Gerald M. Edelman , Wei Zhou
IPC分类号： C07H21/04 , C12N15/63 , C12N15/85 , C12P21/00
摘要： Provided are mRNA translational enhancer elements (TEEs), e.g., SEQ ID NOs:1-35. Also provided are translational enhancer polynucleotides that comprise one or more of the specific TEEs exemplified herein or their variants, homologs or functional derivatives. Further provided are expression vectors comprising such TEEs or translational enhancer polynucleotides, as well as host cells and expression systems that harbor such vectors.

5. 发明授权

US07468275B2 Synthetic internal ribosome entry sites and methods of identifying same 失效
标题翻译：合成内部核糖体进入位点及其鉴定方法
公开(公告)号：US07468275B2
公开(公告)日：2008-12-23
申请号：US10182327
申请日：2001-01-26
申请人： Vincent P. Mauro , Gerald M. Edelman , Stephen A. Chappell , Geoffrey Owens , Jason K. Pinkstaff , Leslie Krushel , Wei Zhou
发明人： Vincent P. Mauro , Gerald M. Edelman , Stephen A. Chappell , Geoffrey Owens , Jason K. Pinkstaff , Leslie Krushel , Wei Zhou
IPC分类号： C12N15/63 , C12N15/85 , C12N5/10 , C07H21/02 , C07H21/04 , A61K48/00
CPC分类号： C12N15/1034 , C12N15/79
摘要： Synthetic and isolated translational regulatory elements, including oligonucleotides that have translational enhancing activity, internal ribosome entry site (IRES) activity, or translational inhibitory activity, and multimers of such translational regulatory elements are provided. In addition, compositions that include such translational regulatory elements are provided, as are methods of using the translational regulatory elements.
摘要翻译：提供合成和分离的翻译调节元件，包括具有翻译增强活性的寡核苷酸，内部核糖体进入位点（IRES）活性或翻译抑制活性，以及这些翻译调节元件的多聚体。此外，提供包括这种翻译调控元件的组合物，以及使用翻译调节元件的方法。

6. 发明授权

US09074220B2 Translation enhancer-element dependent vector systems 有权
公开(公告)号：US09074220B2
公开(公告)日：2015-07-07
申请号：US12857286
申请日：2010-08-16
申请人： Vincent P. Mauro , Gerald M. Edelman , Wei Zhou
发明人： Vincent P. Mauro , Gerald M. Edelman , Wei Zhou
IPC分类号： C12N15/00 , C07H21/02 , C07H21/04 , C12N15/85 , C12N15/10 , C12N15/67
CPC分类号： C12N15/1051 , C12N15/1086 , C12N15/67 , C12N15/85 , C12N2800/107 , C12N2830/002 , C12N2840/105 , C12N2840/20 , C12N2840/203
摘要： A translation enhancer-driven positive feedback vector system is disclosed which is designed to facilitate identification of a Translational Enhancer Element (TEE) and to provide a means for overexpression of gene products. The system exploits both transcriptional and translational approaches to control the expression levels of genes and/or gene products. Methods are also disclosed for screening libraries of random nucleotide sequences to identify translational elements and for overproduction of proteins, which have uses in both research and industrial environments.

7. 发明授权

US08853179B2 Reengineering mRNA primary structure for enhanced protein production 有权
标题翻译：重组mRNA一级结构，增强蛋白质生产
公开(公告)号：US08853179B2
公开(公告)日：2014-10-07
申请号：US13203229
申请日：2010-02-24
申请人： Vincent P. Mauro , Stephen A. Chappell , Wei Zhou , Gerald M. Edelman
发明人： Vincent P. Mauro , Stephen A. Chappell , Wei Zhou , Gerald M. Edelman
IPC分类号： C12N15/11 , C12P21/02
CPC分类号： C12P21/00 , C12N15/111 , C12N15/67 , C12N2310/141 , C12N2320/50 , C12N2320/53 , C12P21/02
摘要： Described herein are rules to modify natural mRNAs or to engineer synthetic mRNAs to increase their translation efficiencies. These rules describe modifications to mRNA coding and 3′ UTR sequences intended to enhance protein synthesis by: 1) decreasing ribosomal diversion via AUG or non-canonical initiation codons in coding sequences, and/or 2) by evading miRNA-mediated down-regulation by eliminating one or more miRNA binding sites in coding sequences.
摘要翻译：本文描述的是修饰天然mRNA或修饰合成mRNA以提高其翻译效率的规则。这些规则描述了通过以下方式来改进mRNA编码和旨在增强蛋白质合成的3'UTR序列：1）通过编码序列中的AUG或非正常起始密码子减少核糖体转移，和/或2）通过逃避miRNA介导的下调消除编码序列中的一个或多个miRNA结合位点。

8. 发明申请

US20120053333A1 Reengineering MRNA Primary Structure For Enhanced Protein Production 有权
标题翻译：重新设计增强蛋白质生产的MRNA主要结构
公开(公告)号：US20120053333A1
公开(公告)日：2012-03-01
申请号：US13203229
申请日：2010-02-24
申请人： Vincent P. Mauro , Stephen A. Chappell , Wei Zhou , Gerald M. Edelman
发明人： Vincent P. Mauro , Stephen A. Chappell , Wei Zhou , Gerald M. Edelman
IPC分类号： C07H21/02 , C07H1/00
CPC分类号： C12P21/00 , C12N15/111 , C12N15/67 , C12N2310/141 , C12N2320/50 , C12N2320/53 , C12P21/02
摘要： Described herein are rules to modify natural mRNAs or to engineer synthetic mRNAs to increase their translation efficiencies. These rules describe modifications to mRNA coding and 3′ UTR sequences intended to enhance protein synthesis by: 1) decreasing ribosomal diversion via AUG or non-canonical initiation codons in coding sequences, and/or 2) by evading miRNA-mediated down-regulation by eliminating one or more miRNA binding sites in coding sequences.
摘要翻译：本文描述的是修饰天然mRNA或修饰合成mRNA以提高其翻译效率的规则。这些规则描述了通过以下方式来改进mRNA编码和旨在增强蛋白质合成的3'UTR序列：1）通过编码序列中的AUG或非正常起始密码子减少核糖体转移，和/或2）通过逃避miRNA介导的下调消除编码序列中的一个或多个miRNA结合位点。

9. 发明申请

US20110124100A1 TRANSLATION ENHANCER-ELEMENT DEPENDENT VECTOR SYSTEMS 有权
标题翻译：翻译增强元素依赖矢量系统
公开(公告)号：US20110124100A1
公开(公告)日：2011-05-26
申请号：US12857286
申请日：2010-08-16
申请人： Vincent P. Mauro , Gerald M. Edelman , Wei Zhou
发明人： Vincent P. Mauro , Gerald M. Edelman , Wei Zhou
IPC分类号： C12N15/63
CPC分类号： C12N15/1051 , C12N15/1086 , C12N15/67 , C12N15/85 , C12N2800/107 , C12N2830/002 , C12N2840/105 , C12N2840/20 , C12N2840/203
摘要： A translation enhancer-driven positive feedback vector system is disclosed which is designed to facilitate identification of a Translational Enhancer Element (TEE) and to provide a means for overexpression of gene products. The system exploits both transcriptional and translational approaches to control the expression levels of genes and/or gene products. Methods are also disclosed for screening libraries of random nucleotide sequences to identify translational elements and for overproduction of proteins, which have uses in both research and industrial environments.
摘要翻译：公开了翻译增强子驱动的正反馈向量系统，其被设计为便于识别翻译增强子元件（TEE）并提供用于过表达基因产物的手段。该系统利用转录和翻译方法来控制基因和/或基因产物的表达水平。还公开了用于筛选随机核苷酸序列文库的方法，以鉴定翻译元件和蛋白质的过量产生，其在研究和工业环境中都有用途。

10. 发明授权

US09068197B2 Translation enhancer-element dependent vector systems 有权
标题翻译：翻译增强子元素相关向量系统
公开(公告)号：US09068197B2
公开(公告)日：2015-06-30
申请号：US11509293
申请日：2006-08-23
申请人： Vincent P. Mauro , Gerald M. Edelman , Wei Zhou
发明人： Vincent P. Mauro , Gerald M. Edelman , Wei Zhou
IPC分类号： C12N15/00 , C07H21/02 , C07H21/04 , C12N15/85 , C12N15/10 , C12N15/67
CPC分类号： C12N15/1051 , C12N15/1086 , C12N15/67 , C12N15/85 , C12N2800/107 , C12N2830/002 , C12N2840/105 , C12N2840/20 , C12N2840/203
摘要： A translation enhancer-driven positive feedback vector system is disclosed which is designed to facilitate identification of a Translational Enhancer Element (TEE) and to provide a means for overexpression of gene products. The system exploits both transcriptional and translational approaches to control the expression levels of genes and/or gene products. Methods are also disclosed for screening libraries of random nucleotide sequences to identify translational elements and for overproduction of proteins, which have uses in both research and industrial environments.
摘要翻译：公开了翻译增强子驱动的正反馈向量系统，其被设计为便于识别翻译增强子元件（TEE）并提供用于过表达基因产物的手段。该系统利用转录和翻译方法来控制基因和/或基因产物的表达水平。还公开了用于筛选随机核苷酸序列文库的方法，以鉴定翻译元件和蛋白质的过量产生，其在研究和工业环境中都有用途。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式