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1. 发明申请

WO2013007820A1 SCREENING METHOD 审中-公开
标题翻译：筛选方法
公开(公告)号：WO2013007820A1
公开(公告)日：2013-01-17
申请号：PCT/EP2012/063802
申请日：2012-07-13
申请人： DSM IP Assets B.V. , VAN RIJ, Evert Tjeerd , RENNIERS, Antonius Cornelis Henrica Maria , PRAGAI, Zoltan , VAN PEIJ, Noël Nicolaas Maria Elisabeth
发明人： VAN RIJ, Evert Tjeerd , RENNIERS, Antonius Cornelis Henrica Maria , PRAGAI, Zoltan , VAN PEIJ, Noël Nicolaas Maria Elisabeth
IPC分类号： C12N15/62 , C12N9/24
CPC分类号： C12N15/625 , C07K2319/00 , C07K2319/036 , C07K2319/50 , C12N9/2414 , C12Y302/01133
摘要： The present invention relates to a method for constructing a nucleotide fusion construct encoding a fusion between a signal peptide and an amino acid sequence of interest, which allows for secretion of the amino acid sequence of interest without modifications to its amino acid sequence.
摘要翻译：本发明涉及构建编码信号肽与感兴趣的氨基酸序列之间的融合物的核苷酸融合构建体的方法，其允许在不修饰其氨基酸序列的情况下分泌目的氨基酸序列。

2. 发明申请

WO1997000964A1 NOVEL BETA-XYLOSIDASE, NUCLEOTIDE SEQUENCE ENCODING IT, AND USE THEREOF 审中-公开
标题翻译：新型β-XYLOSIDASE，核苷酸序列编码及其使用
公开(公告)号：WO1997000964A1
公开(公告)日：1997-01-09
申请号：PCT/NL1996000258
申请日：1996-06-24
申请人： RIJKSLANDBOUWUNIVERSITEIT WAGENINGEN , DE GRAAF, Leendert, Hendrik , VAN PEIJ, Noël, Nicolaas, Maria, Elisabeth , VAN DEN BROECK, Henriëtte, Catharina , VISSER, Jacob
发明人： RIJKSLANDBOUWUNIVERSITEIT WAGENINGEN
IPC分类号： C12N15/56
CPC分类号： C12Y302/01037 , C07K14/38 , C07K14/4705 , C12N9/0006 , C12N9/2437 , C12N9/248 , C12N9/2482 , C12N9/88 , C12N15/1027 , C12N15/63 , C12N15/65 , C12N15/80 , C12Q1/02 , C12Y302/01008 , C12Y302/01055
摘要： A nucleotide sequence is provided which encodes a peptide having beta-xylosidase activity and exhibits at least 30 % amino acid identity with the amino acid sequence shown in SEQ ID NO. 1 or hybridises under stringent conditions with a nucleotide sequence shown in SEQ ID NO. 1, or a part thereof having at least 15 nucleotides encoding an amino acid sequence shown in SEQ ID NO. 1. Also provided is a peptide having beta-xylosidase activity and exhibiting at least 30 % amino acid identity with the amino acid sequence shown in SEQ ID NO. 1 or a part thereof having at least 8 amino acids shown in SEQ ID NO. 1. SEQ ID NO. 1 is derived from Aspergillus niger.
摘要翻译：提供了编码具有β-木糖苷酶活性的肽并与SEQ ID NO：1所示的氨基酸序列呈现至少30％氨基酸同一性的核苷酸序列。 1或在严格条件下与SEQ ID NO：1所示的核苷酸序列杂交。 1或其部分具有编码SEQ ID NO：1所示氨基酸序列的至少15个核苷酸。还提供了具有β-木糖苷酶活性的肽，并且与SEQ ID NO：1所示的氨基酸序列呈现至少30％的氨基酸同一性。 1或其部分具有SEQ ID NO：1所示的至少8个氨基酸。 SEQ ID NO： 1衍生自黑曲霉（Aspergillus niger）。

3. 发明申请

WO2006077258A1 METHOD FOR PRODUCING A COMPOUND OF INTEREST IN A FILAMENTOUS FUNGAL CELL 审中-公开
标题翻译：用于生产在真菌真菌细胞中有利的化合物的方法
公开(公告)号：WO2006077258A1
公开(公告)日：2006-07-27
申请号：PCT/EP2006/050398
申请日：2006-01-24
申请人： DSM IP ASSETS B.V. , ROUBOS, Johannes, Andries , DONKERS, Serge, Petrus , STAM, Hein , VAN PEIJ, Noël, Nicolaas, Maria, Elisabeth
发明人： ROUBOS, Johannes, Andries , DONKERS, Serge, Petrus , STAM, Hein , VAN PEIJ, Noël, Nicolaas, Maria, Elisabeth
IPC分类号： C12N9/20 , C12N9/30 , C12N15/80
CPC分类号： C12N9/242 , C12N9/20 , C12N15/80
摘要： The invention relates to a nucleotide sequence comprising: a synonymous nucleotide coding sequence with optimized codon frequency such that a native codon has been exchanged with a synonymous codon, said synonymous codon encoding the same amino acid as the native codon and having a higher frequency in codon usage as defined in Table 1 than the native codon; and optionally said nucleotide sequence comprises control sequences such as: one translational termination sequence orientated in 5' towards 3' direction selected from the following list of sequences: TAAG, TAGA and TAAA, preferably TAAA, and/or one translational initiator coding sequence orientated in 5' towards 3' direction selected from the following list of sequences: gctnccyyc, using ambiguity codes for nucleotides: v (A/C/G); n (A/C/G/T), preferably 5'-GCT TCC TTC-3'. The invention further relates to a consensus translational initiator sequence: 5'-mwChkyCAmv-3', preferably the translational initiator sequence is selected from the list consisting of: 5'-mwChkyCAAA-3', 5'-mwChkyCACA-3', and 5'-mwChkyCAAG-3'.
摘要翻译：本发明涉及一种核苷酸序列，其包括：具有优化的密码子频率的同义核苷酸编码序列，使得本地密码子已经与同义密码子交换，所述同义密码子编码与天然密码子相同的氨基酸并且在密码子中具有较高的频率用法如表1所定义而不是本地密码子; 并且任选地所述核苷酸序列包括控制序列，例如：从5个朝向3'方向的5'朝向3个方向的一个翻译终止序列，其选自以下的序列：TAAG，TAGA和TAAA，优选TAAA，和/或一个定向于从以下序列列表中选择5'朝向3'方向：gctnccyyc，使用核苷酸的模糊码：v（A / C / G）; n（A / C / G / T），优选5'-GCT TCC TTC-3'。本发明还涉及一个共有的翻译启动子序列：5'-mwChkyCAmv-3'，优选的是翻译起始子序列选自：5'-mwChkyCAAA-3'，5'-mwChkyCACA-3'和5 '-mwChkyCAAG-3'。

4. 发明申请

WO2013007821A1 SCREENING METHOD 审中-公开
标题翻译：筛选方法
公开(公告)号：WO2013007821A1
公开(公告)日：2013-01-17
申请号：PCT/EP2012/063807
申请日：2012-07-13
申请人： DSM IP ASSETS B.V. , VAN RIJ, Evert, Tjeerd , RENNIERS, Antonius, Cornelis, Henrica, Maria , PRAGAI, Zoltan , VAN PEIJ, Noël, Nicolaas, Maria, Elisabeth
发明人： VAN RIJ, Evert, Tjeerd , RENNIERS, Antonius, Cornelis, Henrica, Maria , PRAGAI, Zoltan , VAN PEIJ, Noël, Nicolaas, Maria, Elisabeth
IPC分类号： C12N15/62 , C12N15/63
CPC分类号： C12N15/625 , C07K2319/00 , C07K2319/036 , C07K2319/50 , C12N9/2414 , C12Y302/01133
摘要： The present invention relates to a method for constructing a nucleotide fusion construct encoding a fusion between a signal peptide and an amino acid sequence of interest, which allows for secretion of the amino acid sequence of interest without modifications to its amino acid sequence. The collection of signal peptide encoding nucleotide sequences has been designed in such a way that the collection is widely applicable.
摘要翻译：本发明涉及构建编码信号肽与感兴趣的氨基酸序列之间的融合物的核苷酸融合构建体的方法，其允许在不修饰其氨基酸序列的情况下分泌目的氨基酸序列。已经设计了信号肽编码核苷酸序列的集合，使得该集合被广泛应用。

5. 发明申请

WO2008098933A1 A RECOMBINANT HOST CELL FOR THE PRODUCTION OF A COMPOUND OF INTEREST 审中-公开
标题翻译：用于生产化合物的重组主体细胞
公开(公告)号：WO2008098933A1
公开(公告)日：2008-08-21
申请号：PCT/EP2008/051680
申请日：2008-02-12
申请人： DSM IP ASSETS B.V. , SAGT, Cornelis, Maria, Jacobus , VAN PEIJ, Noël, Nicolaas, Maria, Elisabeth , WÖSTEN, Herman Abel Bernard , COSTA RODRIGUES ALVES, Alexandra Maria , DE VRIES, Ronald, Peter , LEVIN CHUCREL, Ana Marcela
发明人： SAGT, Cornelis, Maria, Jacobus , VAN PEIJ, Noël, Nicolaas, Maria, Elisabeth , WÖSTEN, Herman Abel Bernard , COSTA RODRIGUES ALVES, Alexandra Maria , DE VRIES, Ronald, Peter , LEVIN CHUCREL, Ana Marcela
IPC分类号： C12N15/67 , C12N15/80 , C12N1/14 , C12P1/02 , C12P21/00
CPC分类号： C12N15/80 , C12N9/0061 , C12N9/2428 , C12Y110/03002 , C12Y302/01003
摘要： The present invention relates to a recombinant host cell for the production of a compound of interest. The present invention also relates to isolated fungal promoter DNA sequences, to DNA constructs, vectors, and fungal host cells comprising these promoters in operative association with coding sequences encoding a compound of interest. The present invention also relates to methods for expressing a gene of interest and/or producing compounds of interest using a promoter according to the invention.
摘要翻译：本发明涉及用于生产感兴趣的化合物的重组宿主细胞。本发明还涉及包含这些启动子的DNA构建体，载体和真菌宿主细胞的分离的真菌启动子DNA序列，其与编码感兴趣的化合物的编码序列有效结合。本发明还涉及使用本发明的启动子表达感兴趣的基因和/或产生感兴趣的化合物的方法。

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