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1. 发明申请

WO1997000964A1 NOVEL BETA-XYLOSIDASE, NUCLEOTIDE SEQUENCE ENCODING IT, AND USE THEREOF 审中-公开
标题翻译：新型β-XYLOSIDASE，核苷酸序列编码及其使用
公开(公告)号：WO1997000964A1
公开(公告)日：1997-01-09
申请号：PCT/NL1996000258
申请日：1996-06-24
申请人： RIJKSLANDBOUWUNIVERSITEIT WAGENINGEN , DE GRAAF, Leendert, Hendrik , VAN PEIJ, Noël, Nicolaas, Maria, Elisabeth , VAN DEN BROECK, Henriëtte, Catharina , VISSER, Jacob
发明人： RIJKSLANDBOUWUNIVERSITEIT WAGENINGEN
IPC分类号： C12N15/56
CPC分类号： C12Y302/01037 , C07K14/38 , C07K14/4705 , C12N9/0006 , C12N9/2437 , C12N9/248 , C12N9/2482 , C12N9/88 , C12N15/1027 , C12N15/63 , C12N15/65 , C12N15/80 , C12Q1/02 , C12Y302/01008 , C12Y302/01055
摘要： A nucleotide sequence is provided which encodes a peptide having beta-xylosidase activity and exhibits at least 30 % amino acid identity with the amino acid sequence shown in SEQ ID NO. 1 or hybridises under stringent conditions with a nucleotide sequence shown in SEQ ID NO. 1, or a part thereof having at least 15 nucleotides encoding an amino acid sequence shown in SEQ ID NO. 1. Also provided is a peptide having beta-xylosidase activity and exhibiting at least 30 % amino acid identity with the amino acid sequence shown in SEQ ID NO. 1 or a part thereof having at least 8 amino acids shown in SEQ ID NO. 1. SEQ ID NO. 1 is derived from Aspergillus niger.
摘要翻译：提供了编码具有β-木糖苷酶活性的肽并与SEQ ID NO：1所示的氨基酸序列呈现至少30％氨基酸同一性的核苷酸序列。 1或在严格条件下与SEQ ID NO：1所示的核苷酸序列杂交。 1或其部分具有编码SEQ ID NO：1所示氨基酸序列的至少15个核苷酸。还提供了具有β-木糖苷酶活性的肽，并且与SEQ ID NO：1所示的氨基酸序列呈现至少30％的氨基酸同一性。 1或其部分具有SEQ ID NO：1所示的至少8个氨基酸。 SEQ ID NO： 1衍生自黑曲霉（Aspergillus niger）。

2. 发明申请

WO1997013862A1 FUNGAL CELLULASES 审中-公开
标题翻译：真菌细胞
公开(公告)号：WO1997013862A1
公开(公告)日：1997-04-17
申请号：PCT/EP1996004511
申请日：1996-10-14
申请人： GIST-BROCADES B.V. , VAN DEN BROECK, Henriëtte, Catharina , DE GRAAFF, Leendert, Hendrik , VISSER, Jacob , VAN OOYEN, Albert, Johannes, Joseph
发明人： GIST-BROCADES B.V.
IPC分类号： C12N15/56
CPC分类号： C12N9/2437 , C12Y302/01004
摘要： The present invention provides polypeptides with cellulase activity, corresponding DNA sequences, vectors, transformed hosts and a method for the production of these polypeptides, which are obtainable from Aspergillus niger var. niger or Aspergillus niger var. tubigensis.
摘要翻译：本发明提供具有纤维素酶活性的多肽，相应的DNA序列，载体，转化的宿主和用于制备这些多肽的方法，其可从黑曲霉变种（Aspergillus niger var。黑曲霉或黑曲霉变种塔宾。

3. 发明申请

WO1997000962A1 A NOVEL METHOD TO ISOLATE MUTANTS AND TO CLONE THE COMPLEMENTING GENE 审中-公开
标题翻译：一种分离突变体并克隆完整基因的新方法
公开(公告)号：WO1997000962A1
公开(公告)日：1997-01-09
申请号：PCT/NL1996000259
申请日：1996-06-24
申请人： RIJKSLANDBOUWUNIVERSITEIT WAGENINGEN , DE GRAAFF, Leendert, Hendrik , VAN DEN BROECK, Henriëtte, Catharina , VISSER, Jacob
发明人： RIJKSLANDBOUWUNIVERSITEIT WAGENINGEN
IPC分类号： C12N15/31
CPC分类号： C12N15/635 , C07K14/38 , C07K14/4705 , C12N9/0006 , C12N9/2434 , C12N9/248 , C12N9/88 , C12N15/1027 , C12N15/63 , C12N15/65 , C12N15/80 , C12Q1/02 , C12Y302/01003 , C12Y302/01008 , C12Y302/01015 , C12Y302/01037 , C12Y302/01055 , C12Y302/01099
摘要： The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. A nucleic acid cassette comprising a nucleic acid sequence encoding a bidirectional marker, said nucleic acid cassette further comprising a basic transcriptional unit operatively linked to the nucleic acid sequence encoding the bidirectional marker and said nucleic acid cassette further comprising an inducible enhancer or activator sequence linked to the basic transcription unit in such a manner that upon induction of the enhancer or activator sequence the bidirectional marker encoding nucleic acid sequence is expressed, said inducible enhancer or activator sequence being derived from a gene associated with activity of part of metabolism, said inducible enhancer or activator sequence being derived from a gene associated with metabolism is claimed as is application thereof in a selection method for mutants. In addition a regulator gene x1nR encoding an activating regulator of an inducible enhancer or activator sequence and application of said gene and/or its expression product in overexpression of homologous or heterologous protein or peptide is described. Knockout mutants wherein said gene is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.
摘要翻译：本发明在于微生物突变和突变体的选择领域。特别地，本发明涉及以简单，直接和具体的方式获得代谢突变体。在优选的实施方案中，由于较短的立法程序，也可以获得不包含重组DNA的所需突变体，从而便于将其并入用于人类消费或应用的产品中。根据本发明的方法涉及随机突变和所需代谢突变体的特异性选择。一种核酸盒，其包含编码双向标记的核酸序列，所述核酸盒还包含与编码双向标记的核酸序列可操作地连接的碱性转录单位，并且所述核酸盒还包含可诱导的增强子或活化剂序列所述基本转录单元以这样的方式，即在诱导增强子或活化剂序列时，表达编码双向标记核酸序列的核酸序列，所述诱导型增强子或活化子序列衍生自与部分代谢活性相关的基因，所述诱导型增强子或要求从与代谢相关的基因衍生的活化剂序列作为其在突变体的选择方法中的应用。此外，还描述了编码诱导型增强子或激活物序列的活化调节物的调节基因x1nR以及所述基因和/或其表达产物在同源或异源蛋白质或肽的过表达中的应用。其中所述基因不存在或失活的敲除突变体以及DNA结合能力增加或降低的突变体也被要求保护。

4. 发明申请

WO1997013853A2 PROTEIN DETECTION 审中-公开
标题翻译：蛋白质检测
公开(公告)号：WO1997013853A2
公开(公告)日：1997-04-17
申请号：PCT/EP1996004510
申请日：1996-10-14
申请人： GIST-BROCADES B.V. , VAN DEN BROECK, Henriëtte, Catharina , DE GRAAFF, Leendert, Hendrik , VISSER, Jacob , VAN OOYEN, Albert, Johannes, Joseph
发明人： GIST-BROCADES B.V.
IPC分类号： C12N15/10
CPC分类号： C12N15/1086 , C12N9/2437 , C12N9/248 , C12Y302/01004 , C12Y302/01008 , C12Y302/01032 , C12Y302/01055
摘要： The present invention provides a method for identifying a DNA fragment encoding a protein of interest, wherein a cDNA library in the form of bacterial host cells transformed with DNA obtainable from an eukaryotic organism capable of producing said protein is screened for expression of said protein using a test which is indicative for its presence, characterised in that screening of the host cells is performed after the transforming DNA has become part of a plasmid. The cDNA library is preferably from an eukaryotic organism. The eukaryotic organism is preferably a fungus or a yeast cell, more preferably an Aspergillus species, most preferred is a member of the Aspergillus niger group. The enzymes screened for are preferably enzymes which show cellulase, endoxylanase or arabinoxylan degrading activity.
摘要翻译：本发明提供了一种鉴定编码目的蛋白质的DNA片段的方法，其中筛选以能从产生所述蛋白质的真核生物获得的DNA转化的细菌宿主细胞形式的cDNA文库，以使用测试其指示其存在，其特征在于在转化DNA已经成为质粒的一部分之后进行宿主细胞的筛选。 cDNA文库优选来自真核生物。真核生物优选为真菌或酵母细胞，更优选为曲霉菌种，最优选为黑曲霉组的成员。筛选的酶优选是显示纤维素酶，内切木聚糖酶或阿拉伯木聚糖降解活性的酶。

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