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    • 1. 再颁专利
    • Process and arrangement for confocal microscopy
    • 共焦显微镜的工艺和布置
    • USRE41666E1
    • 2010-09-14
    • US10959949
    • 2004-10-07
    • Ulrich SimonSebastian TilleGunter MoehlerStefan WilhelmUlrich MeiselErnst Stelzer
    • Ulrich SimonSebastian TilleGunter MoehlerStefan WilhelmUlrich MeiselErnst Stelzer
    • G01N21/64G01J3/30
    • G02B21/0056G01N21/645G02B21/0064G02B21/0076G02B21/0084
    • A process for confocal microscopy is disclosed in which laser light is coupled into a microscope beam path, directed successively with respect to time onto different locations of a specimen, and an image of the scanned plane is generated from the light reflected and emitted by the irradiated locations. A change in the spectral composition and in the intensity of light is are carried out during the deflection of the laser beam from location to location, while the deflection continues in an uninterrupted manner. In this way , so that at least two adjacent locations of the specimen located next to one another are acted upon by light with different spectral characteristics and by laser radiation of different intensity. By periodically interrupting the coupling in of the laser light during the deflection of the microscope beam path, it is made possible that only selected portions of the image field are acted upon by the laser radiation. A laser scanning microscope for carrying out this process is also disclosed. A laser scanning microscope for carrying out this process is also disclosed.
    • 公开了一种用于共焦显微镜的方法,其中将激光耦合到相对于时间相对于样本的不同位置的显微镜光束路径中,并且从被照射的反射和发射的光产生扫描平面的图像 位置。 在激光束从位置到位置的偏转期间,进行光谱组成和光强度的变化,同时偏转以不间断的方式继续。 以这种方式,使得彼此相邻的样本的至少两个相邻位置由具有不同光谱特性的光和不同强度的激光辐射作用。 通过在显微镜光束路径的偏转期间周期性地中断激光的耦合,使得只有图像场的选定部分被激光辐射作用才有可能。 还公开了用于进行该方法的激光扫描显微镜。 还公开了用于进行该方法的激光扫描显微镜。
    • 4. 发明授权
    • Process and arrangement for confocal microscopy
    • 共焦显微镜的工艺和布置
    • US06462345B1
    • 2002-10-08
    • US09295556
    • 1999-04-21
    • Ulrich SimonSebastian TilleGunter MoehlerStefan WilhelmUlrich MeiselErnst Hans Karl Stelzer
    • Ulrich SimonSebastian TilleGunter MoehlerStefan WilhelmUlrich MeiselErnst Hans Karl Stelzer
    • G01N2164
    • G02B21/0056G01N21/645G02B21/0064G02B21/0076G02B21/0084
    • A process for confocal microscopy is disclosed in which laser light is coupled into a microscope beam path, directed successively with respect to time onto different locations of a specimen, and an image of the scanned plane is generated from the light reflected and emitted by the irradiated locations. A change in the spectral composition and in the intensity of light is carried out during the deflection of the laser beam from location to location, while the deflection continues in an uninterrupted manner. In this way, at least two locations of the specimen located next to one another are acted upon by light with different spectral characteristics and by laser radiation of different intensity. By periodically interrupting the coupling in of the laser light during the deflection of the microscope beam path, it is made possible that only selected portions of the image field are acted upon by the laser radiation. A laser scanning microscope for carrying out this process is also disclosed.
    • 公开了一种用于共焦显微镜的方法,其中将激光耦合到相对于时间相对于样本的不同位置的显微镜光束路径中,并且从被照射的反射和发射的光产生扫描平面的图像 位置。 在激光束从位置到位置的偏转期间进行光谱组成和光强度的变化,而偏转以不间断的方式继续。 以这种方式,彼此相邻的样本的至少两个位置由具有不同光谱特性的光和不同强度的激光辐射作用。 通过在显微镜光束路径的偏转期间周期性地中断激光的耦合,使得只有图像场的选定部分被激光辐射作用才有可能。 还公开了用于进行该方法的激光扫描显微镜。