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1. 发明授权

US6004743A Method and apparatus for bulk enrichment of a population or subpopulation of cells 失效
标题翻译：用于大量富集细胞群体或亚群体的方法和装置
公开(公告)号：US6004743A
公开(公告)日：1999-12-21
申请号：US989124
申请日：1997-12-10
申请人： Norma S. Kenyon , Thomas R. Russell , Camillo Ricordi , Robert K. Zwerner
发明人： Norma S. Kenyon , Thomas R. Russell , Camillo Ricordi , Robert K. Zwerner
IPC分类号： G01N33/53 , C12N5/06 , G01N33/537 , G01N33/543 , G01N33/553 , G01N33/569 , A01N1/02 , G01N33/567
CPC分类号： G01N33/5375 , G01N33/54313 , G01N33/553 , G01N33/56966 , G01N33/56972
摘要： A cell population or subpopulation enrichment procedure for separating undesired populations or subpopulations from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles have one or more reactants bound thereto which are specific to and will bind with the selected population or subpopulation to be eliminated from the sample. The particles preferably are mixed with the sample by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population to be eliminated then are allowed to preferentially settle in the sample and the supernatant including the enriched population or subpopulation is separated from the particles with the population to be eliminated bound thereto. The enriched population supernatant can then be analyzed, utilized as is, have other populations or subpopulations removed or have additional amounts of remaining cells of the previously eliminated populations removed in additional steps.
摘要翻译：使用相对重的致密颗粒和重力沉降从生物样品中分离不想要的种群或亚群体的细胞群或亚群富集程序。颗粒具有一个或多个与其结合的反应物，其与所选择的群体或子群体特异并将与样品中的氨基酸结合。颗粒优选通过重复使颗粒沉淀通过样品的大部分与样品结合而与样品混合。将具有待排除的结合的选定群体的颗粒允许优先沉淀在样品中，并将包含富集群体或亚群体的上清液与要与其排除的群体结合的颗粒分离。然后可以分析富集的群体上清液，按其他方式使用，除去其他群体或亚群体，或者在额外的步骤中除去先前消除的群体的额外量的剩余细胞。

2. 发明授权

US09790539B2 Methods and reagents for improved selection of biological molecules 有权
公开(公告)号：US09790539B2
公开(公告)日：2017-10-17
申请号：US11159957
申请日：2005-06-23
申请人： Thomas R. Russell , Michael J. Ciocci , Michael Musick
发明人： Thomas R. Russell , Michael J. Ciocci , Michael Musick
IPC分类号： C12Q1/68 , G01N33/543
CPC分类号： C12Q1/6806 , C12Q1/6804 , G01N33/54313 , G01N33/54326 , G01N33/5434
摘要： Coated Ferromagnetic Density Particles or Density Particles with binding agents bound thereto capable of binding biological molecules and methods of use and apparatus for means are disclosed. Coated particles coupled to specific binding agents can be used for molecular biology and proteomic applications in research and diagnostics.

3. 发明授权

US5576185A Method of positive or negative selection of a population or subpopulation of a sample utilizing particles and gravity sedimentation 失效
标题翻译：使用颗粒和重力沉降的样品的群体或亚群的正或负选择方法
公开(公告)号：US5576185A
公开(公告)日：1996-11-19
申请号：US228791
申请日：1994-04-15
申请人： Wallace H. Coulter , Robert K. Zwerner , Robert J. Schmittling , Thomas R. Russell
发明人： Wallace H. Coulter , Robert K. Zwerner , Robert J. Schmittling , Thomas R. Russell
IPC分类号： B01D21/00 , G01N33/543 , G01N33/553 , G01N33/569 , G01N33/574
CPC分类号： G01N33/54333 , G01N33/553 , G01N33/56972 , G01N33/574 , Y10S436/824
摘要： A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including the non-selected population is separated from the particles with the selected population bound thereto. The particles can be heated for sterilization and endotoxin removal.
摘要翻译：用于使用相对重的，致密的颗粒和重力沉降从生物样品中分离所选择的所需或不需要的群体的分离程序。颗粒具有一个或多个与其结合的反应物，其特异于并将与所选群体结合。颗粒优选通过重复使颗粒沉淀通过样品的大部分与样品结合而与样品混合。允许具有结合的所选群体的颗粒优先沉降在样品中，并且将包含未选择群体的上清液与所选择的群体结合的颗粒分离。可以加热颗粒进行灭菌和去除内毒素。

4. 发明授权

US5437985A Conservative whole blood sample preparation technique 失效
标题翻译：保守的全血样品制备技术
公开(公告)号：US5437985A
公开(公告)日：1995-08-01
申请号：US726467
申请日：1991-07-08
申请人： Jorge A. Quintana , Thomas R. Russell , Ronald D. Paul , Timothy J. Fischer
发明人： Jorge A. Quintana , Thomas R. Russell , Ronald D. Paul , Timothy J. Fischer
IPC分类号： A01N1/02 , G01N1/30 , G01N33/48 , G01N33/50 , G01N33/53 , G01N33/543 , G01N33/569 , G01N33/58
CPC分类号： G01N33/56972 , G01N1/30 , G01N33/5094 , Y10S435/962 , Y10T436/25125 , Y10T436/2525
摘要： The method of this invention is directed to the rapid preparation of a whole blood sample for photooptical analysis. In the preferred embodiments of this method, a whole blood sample, lytic reagent system and immunological stain (optional) are contacted with the sample in a common reaction vessel (i.e. cuvette or test tube), with gentle asymmetric vortex mixing, so as to maintain the particulate matter of the sample at an essentially homogeneous concentration throughout the sample. An aliquot of the contents of the reaction vessel can, thereafter, be analyzed for identification and/or quantification of the analyte of interest. This process is conservative of the various endogenous constituents of the sample since virtually all of the manipulative steps involved in the preparation of the sample occur within a common vessel and any excess (unconsumed or unreacted) reagents and stain need not be separated from the sample during such preparation, thus, avoiding the multiple wash steps traditionally associated with this process. This process is uniquely applicable to the conservative preparation of aged whole blood samples (samples in which the endogenous nutrients have been essentially completely consumed) and whole blood samples from diseased state patients wherein the disease is manifest by changes in the morphology, total number and/or relative concentration of one or more of the lymphocyte sub-populations (i.e. B and/or T-cells).
摘要翻译：本发明的方法涉及用于光学光学分析的全血样品的快速制备。在该方法的优选实施方案中，将全血样品，溶解试剂系统和免疫染色剂（任选的）在常规反应容器（即比色杯或试管）中与样品接触，并进行温和的不对称涡旋混合，以保持在整个样品中样品的颗粒物质基本上均匀。然后分析反应容器的内容物的等分试样，以便鉴定和/或定量所关注的分析物。该过程对于样品的各种内生成分是保守的，因为实际上所有的样品制备中所涉及的所有操作步骤都发生在共同的容器内，任何过量的（未消耗的或未反应的）试剂和污渍都不需要从样品中分离出来因此，避免了与该过程相关的多次洗涤步骤。该方法独特地适用于老年全血样品（其中内源性营养素基本上完全消耗的样品）的保守制剂和来自患病状态患者的全血样品，其中疾病由形态学，总数和/ 或一个或多个淋巴细胞亚群（即B和/或T细胞）的相对浓度。

5. 发明授权

US5030554A Conservative whole blood sample preparation technique 失效
标题翻译：保守的全血样品制备技术
公开(公告)号：US5030554A
公开(公告)日：1991-07-09
申请号：US128668
申请日：1987-12-04
申请人： Jorge A. Quintana , Thomas R. Russell , Ronald D. Paul , Timothy J. Fischer
发明人： Jorge A. Quintana , Thomas R. Russell , Ronald D. Paul , Timothy J. Fischer
IPC分类号： A01N1/02 , G01N1/30 , G01N33/48 , G01N33/50 , G01N33/53 , G01N33/543 , G01N33/569 , G01N33/58
CPC分类号： G01N33/56972 , G01N1/30 , G01N33/5094 , Y10S435/962 , Y10T436/25125 , Y10T436/2525
摘要： The method of this invention is directed to the rapid preparation of a whole blood sample for photooptical analysis. In the preferred embodiments of this method, a whole blood sample, lytic reagent system and immunological stain (optional) are contacted with the sample in a common reaction vessel (i.e. cuvette or test tube), with gentle asymmetric vortex mixing, so as to maintain the particulate matter of the sample at an essentially homogeneous concentration throughout the sample. An aliquot of the contents of the reaction vessel can, thereafter, be analyzed for identification and/or quantification of the analyte of interest. This process is conservative of the various endogenous constituents of the sample since virtually all of the manipulative steps involved in the preparation of the sample occur within a common vessel and any excess (unconsumed or unreacted) reagents and stain need not be separated from the sample during such preparation, thus, avoiding the multiple wash steps traditionally associated with this process. This process is uniquely applicable to the conservative preparation of aged whole blood samples (samples in which the endogenous nutrients have been essentially completely consumed) and whole blood samples from diseased state patients wherein the disease is manifest by changes in the morphology, total number and/or relative concentration of one or more of the lymphocyte subpopulations (i.e. B and/or T-cells).
摘要翻译：本发明的方法涉及用于光学光学分析的全血样品的快速制备。在该方法的优选实施方案中，将全血样品，溶解试剂系统和免疫染色剂（任选的）在常规反应容器（即比色杯或试管）中与样品接触，并进行温和的不对称涡旋混合，以保持在整个样品中样品的颗粒物质基本上均匀。然后分析反应容器的内容物的等分试样，以便鉴定和/或定量所关注的分析物。该过程对于样品的各种内生成分是保守的，因为实际上所有的样品制备中所涉及的所有操作步骤都发生在共同的容器内，任何过量的（未消耗的或未反应的）试剂和污渍都不需要从样品中分离出来因此，避免了与该过程相关的多次洗涤步骤。该方法独特地适用于老年全血样品（其中内源性营养素基本上完全消耗的样品）的保守制剂和来自患病状态患者的全血样品，其中疾病由形态学，总数和/ 或相对浓度的一个或多个淋巴细胞亚群（即B和/或T细胞）。

6. 发明授权

US09435799B2 Methods and reagents for improved selection of biological materials 有权
标题翻译：改善生物材料选择的方法和试剂
公开(公告)号：US09435799B2
公开(公告)日：2016-09-06
申请号：US10208939
申请日：2002-07-31
申请人： Thomas R Russell , Pauline McGann , Mike Musick , Michael Ciocci
发明人： Thomas R Russell , Pauline McGann , Mike Musick , Michael Ciocci
IPC分类号： G01N33/543
CPC分类号： G01N33/5434 , G01N33/54326 , G01N33/54346 , G01N33/54353 , G01N33/553 , G01N33/577 , G01N2440/32
摘要： Methods, apparatus and compositions for separating a desired or undesired population or subpopulation from a biological sample are disclosed herein. The selection procedure is based on ferromagnetic, dense particles in a preferred size range from about 0.8 to about 1.2 microns. Specific binding agents are bound to the particles that recognize and bind to specific molecules on the targeted population or subpopulation, and the particles are mixed with the sample in such a way as to promote movement of the particles relative to the sample, promoting binding to the targeted population or subpopulation without non-specifically binding to non-targeted populations in the sample. Because of the large particle density, the bound population is separated from the fluid sample by gravity. Alternatively, the sample, including the bound, targeted population, is placed in a magnetic field such that the particles separate from the sample by evenly distributing over the vessel wall thus limiting non-specific trapping of the non-targeted population.
摘要翻译：本文公开了用于从生物样品中分离所需或不需要的群体或亚群体的方法，装置和组合物。选择程序基于约0.8至约1.2微米的优选尺寸范围内的铁磁致密颗粒。特异性结合剂与识别并结合目标群体或亚群体上的特定分子的颗粒结合，并且颗粒与样品混合以促进颗粒相对于样品的移动，促进与目标群体或亚群，而不特异性地结合样本中的非目标群体。由于粒子密度较大，结合的群体通过重力与流体样本分离。或者，将包括结合的目标群体的样品置于磁场中，使得颗粒通过均匀分布在血管壁上而与样品分离，从而限制非目标群体的非特异性捕获。

7. 发明授权

US06900029B1 Method of selection of a population or subpopulation of a sample utilizing particles and gravity sedimentation 失效
标题翻译：选择使用粒子和重力沉降的样品群体或亚群体的方法
公开(公告)号：US06900029B1
公开(公告)日：2005-05-31
申请号：US08556667
申请日：1995-11-13
申请人： Wallace H. Coulter , Robert K. Zwerner , Robert J. Schmittling , Thomas R. Russell
发明人： Wallace H. Coulter , Robert K. Zwerner , Robert J. Schmittling , Thomas R. Russell
IPC分类号： B01D21/00 , G01N33/543 , G01N33/553 , G01N33/569 , G01N33/574 , A01N1/02 , C12Q1/18 , G01N33/53
CPC分类号： G01N33/54333 , G01N33/553 , G01N33/56972 , G01N33/574 , Y10S436/824
摘要： A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including an enriched population is separated from the particles with the selected population bound thereto. The enriched populations in the biological sample supernatant can be further enriched by multiple removal steps.
摘要翻译：用于使用相对重的，致密的颗粒和重力沉降从生物样品中分离所选择的所需或不需要的群体的分离程序。颗粒具有一个或多个与其结合的反应物，其特异于并将与所选群体结合。颗粒优选通过重复使颗粒沉淀通过样品的大部分与样品结合而与样品混合。然后允许具有结合的所选群体的颗粒优先沉降在样品中，并且将包含富集群体的上清液从结合有所选群体的颗粒中分离。通过多次去除步骤，可以进一步富集生物样品上清液中的富集群体。

8. 发明授权

US5607565A Apparatus for measuring analytes in a fluid sample 失效
标题翻译：用于测量流体样品中分析物的装置
公开(公告)号：US5607565A
公开(公告)日：1997-03-04
申请号：US410997
申请日：1995-03-27
申请人： Roobik Azarnia , Susan M. Fuentes , Thomas R. Russell
发明人： Roobik Azarnia , Susan M. Fuentes , Thomas R. Russell
IPC分类号： G01N27/416 , C12M1/40 , C12Q1/00 , G01N27/28 , G01N27/26
CPC分类号： C12Q1/006 , C12Q1/00 , G01N27/3271 , Y10S435/817 , Y10T436/117497
摘要： A method and apparatus provide a system for measuring a measurable component in a sample. A sample is introduced into a chamber. A permeable membrane to the measurable component allows the component to pass into a second chamber. In the second chamber the component is measured directly or reacted in the presence of an enzyme to generate a detectable product detected by a detector therein. The second chamber is then flushed. The second chamber is only of a size sufficient to contain enough liquid to wet the detector therein.
摘要翻译：方法和装置提供用于测量样品中的可测量组分的系统。将样品引入室中。可测量部件的可渗透膜允许部件进入第二腔室。在第二室中，直接测量组分或在酶的存在下反应以产生由其中的检测器检测到的可检测产物。然后第二个房间被冲洗。第二室仅具有足以容纳足够的液体以使其中的检测器湿润的尺寸。

9. 发明授权

US4892292A Non-corrosive security railing 失效
标题翻译：无腐蚀性安全栏杆
公开(公告)号：US4892292A
公开(公告)日：1990-01-09
申请号：US862252
申请日：1986-05-12
申请人： Thomas R. Russell
发明人： Thomas R. Russell
IPC分类号： E04H17/14 , E04F11/18
CPC分类号： E04F11/181
摘要： A security railing formed from plastics material pipe which is lightweight, low cost, and non-corrosive. The top railing and preferably the posts of the security railing are formed of double pipe construction comprising a first pipe and a second pipe inside the first pipe, the second pipe having an outside diameter related to the inside diameter of the first pipe. The first pipe is of plastics material and the second pipe preferably also is of plastics material. A lower railing utilizes a plastic pipe having the same outside diameter as the laminated top railing. T connectors and cross connectors are used at the joints between the upper and lower railings and the posts, and these T connectors and cross connectors are of the same outer dimension as the first pipe to give the railing a smooth contour. A plurality of holes in the lower surface of the upper railing and the upper surface of the lower railing permit the insertion of smaller diameter plastics pipe sections at closely spaced intervals to provide vertical railing elements. The security railing meets requirements of national building codes and will not permanently deform under test loads. The plastics material used is preferably polyvinyl chloride.

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